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Akrilamidin Koryoallantoik Membran Modelinde Oksidatif ve Anti-Anjiogenik Etkileri

Year 2023, Volume: 9 Issue: 2, 162 - 168, 01.05.2023

Mehmet Enes Sözen Özgür Akkaya Hasan Basri Savaş Oğuz Karahan

https://doi.org/10.53394/akd.1015590
Cited By: 2

Abstract

Amaç: Akrilamid (ACR), karbonhidrat içeren gıdaların yüksek ısıya maruz bırakılarak hazırlanması sırasında oluşur ve patates cipsi, bisküvi, kraker ve ekmek gibi işlenmiş hazır gıdalarda çok miktarda bulunur. ACR toksik bir maddedir ve oksidatif stresi arttırır. Bu çalışmanın amacı, farklı dozlarda akrilamid maruziyetinin korioallantoik membran modelinde anjiyogenez ve oksidan-antioksidan dengesi üzerindeki etkilerini göstermektir.
Yöntemler: İki farklı konsantrasyonda akrilamid hazırlandı (10-3 M, 10-4 M). Yumurtaların korioallantoik membranına peletler yerleştirildi. Deney öncesi ve sonrasında döllenmiş tavuk yumurtalarından sıvı numuneler alındı.
Bulgular: ACR, doza bağlı antianjiyogenik etkiye sahiptir. 10-3 M ACR ve 10-4 M ACR gruplarında kontrol grubuna göre Total oksidan kapasite (TOK) ve Oksidatif stres indeksi (OSI) değerlerinde anlamlı artış var. 10-3 M ACR ve 10-4 M ACR grupları arasında TOK ve OSİ değerlerinde sayısal bir artış olsa da bu artış istatistiksel olarak anlamlı düzeyde değildir.
Sonuç: Bu araştırma, ACR'nin antianjiyogenik etkilerini araştıran ilk çalışmadır ve CAM modelinde oksidatif stresi araştıran ilk çalışmalardan biridir. ACR maruziyeti CAM modelinde oksidatif stresi artırmış ve antianjiogenik etki göstermiştir.

Keywords

Koryoallantoik membran modeli akrilamid oksidan antioksidan anjiyogenez.

References

  • Semla M, Goc Z, Martiniaková M, Omelka R, Formicki G. Acrylamide: a common food toxin related to physiological functions and health. Physiol Res. 2017 May 4;66(2):205-217. doi: 10.33549/physiolres.933381. Epub 2016 Dec 16. PMID: 27982682.
  • Smith CJ, Perfetti TA, Rumple MA, Rodgman A, Doolittle DJ. "IARC group 2A Carcinogens" reported in cigarette mainstream smoke. Food Chem Toxicol. 2000 Apr;38(4):371-83. doi: 10.1016/s0278-6915(99)00156-8. PMID: 10722891.
  • Hong Y, Nan B, Wu X, Yan H, Yuan Y. Allicin alleviates acrylamide-induced oxidative stress in BRL-3A cells. Life Sci. 2019;15;231:116550. doi: 10.1016/j.lfs.2019.116550.
  • Ibrahim MA, Ibrahem MD. Acrylamide-induced hematotoxicity, oxidative stress, and DNA damage in liver, kidney, and brain of catfish (Clarias gariepinus). Environ Toxicol. 2020;35(2):300-308. doi: 10.1002/tox.22863.
  • Das AB, Srivastav PP. Acrylamide in snack foods. Toxicol Mech Methods. 2012 Apr;22(3):163-9. doi: 10.3109/15376516.2011.623329. Epub 2011 Oct 24. PMID: 22023505.
  • Santhanasabapathy R, Vasudevan S, Anupriya K, Pabitha R, Sudhandiran G. Farnesol quells oxidative stress, reactive gliosis and inflammation during acrylamide-induced neurotoxicity: Behavioral and biochemical evidence. Neuroscience. 2015 Nov 12;308:212-27. doi: 10.1016/j.neuroscience.2015.08.067. Epub 2015 Sep 1. PMID: 26341906.
  • Sumizawa T, Igisu H. Apoptosis induced by acrylamide in SH-SY5Y cells. Arch Toxicol. 2007 Apr;81(4):279-82. doi: 10.1007/s00204-006-0145-6. Epub 2006 Aug 25. PMID: 16932918.
  • Gumral N, Saygin M, Asci H, Uguz AC, Celik O, Doguc DK, Savas HB, Comlekci S. The effects of electromagnetic radiation (2450 MHz wireless devices) on the heart and blood tissue: role of melatonin. Bratisl Med J 2016;117(11):665–671. DOI: 10.4149/BLL_2016_128.
  • Savas HB, Sayar E. Oxidant Antioxidant Balance and Trace Elements In Children with Functional Dyspepsia. Turkiye Klinikleri J Med Sci. 2021;41(1):70-9. doi: 10.5336/medsci.2020-79596
  • Savas HB, Sayar E, Kara T. Thiol Disulfide Balance Oxidative Stress and Paraoxonase 1 Activities in Children and Adolescents Aged 6-16 Years with Specific Learning Disorders. Electron J Gen Med. 2021;18(3):em290. https://doi.org/10.29333/ejgm/10837
  • Savran M, Asci H, Ozmen O, Erzurumlu Y, Savas HB, Sonmez Y, Sahin Y. Melatonin protects the heart and endothelium against high fructose corn syrup consumption- induced cardiovascular toxicity via SIRT-1 signaling. Hum Exp Toxicol. 2019;38(10):1212-1223. doi: 10.1177/0960327119860188.
  • Savran M, Aslankoc R, Ozmen O, Erzurumlu Y, Savas HB, Temel EN, Kosar PA, Boztepe S. Agomelatine could prevent brain and cerebellum injury against LPS- induced neuroinflammation in rats. Cytokine. 2020;20;127:154957. doi: 10.1016/j.cyto.2019.154957.
  • Asci H, Ozmen O, Erzurumlu Y, Savas HB, Temel EN, Icten P, Hasseyid N. Ameliorative effects of pregabalin on LPS induced endothelial and cardiac toxicity. Biotech Histochem. 2020;14:1-12. doi: 10.1080/10520295.2020.1810315.
  • D. Ribatti, “The chick embryo chorioallantoic membrane (CAM) assay,” Reprod. Toxicol., vol. 70, pp. 97–101, 2017, doi: 10.1016/j.reprotox.2016.11.004.
  • Nowak-Sliwinska P, Segura T, Iruela-Arispe ML. The chicken chorioallantoic membrane model in biology, medicine and bioengineering. Angiogenesis. 2014;17(4):779-804. doi: 10.1007/s10456-014-9440-7.
  • Kadawathagedara M, Tong ACH, Heude B, Forhan A, Charles MA, Sirot V, Botton J, The Eden Mother-Child Cohort Study Group. Dietary acrylamide intake during pregnancy and anthropometry at birth in the French EDEN mother-child cohort study. Environ Res. 2016;149:189-196. doi: 10.1016/j.envres.2016.05.019. Epub 2016 May 19. PMID: 27208470.
  • Karahan O, Yavuz C, Demirtas S, Caliskan A, Atahan E. The investigation of the antiangiogenic potential of amiodarone HCl in the chick embryo chorioallantoic membrane model. Biomedical Research 2013;24:131-4.
  • Demirtas S, Caliskan A, Guclu O, Yazici S, Karahan O, Yavuz C, Mavitas B. Can calcium dobesilate be used safely for peripheral microvasculopathies that require neoangiogenesis? Med Sci Monit Basic Res. 2013;19:253-7. doi: 10.12659/MSMBR.889427.
  • Aksoy F, Aydın Bas H, A Bagcı, Savas HB. Predictive value of oxidant and antioxidant status for contrast-induced nephropathy after percutaneous coronary intervention for ST-segment elevation myocardial infarction. Rev Port Cardiol. 2020;30:S0870-2551(20)30494-7. doi: 10.1016/j.repc.2020.08.010.
  • Savas HB, Gultekin F, Ciris İM. Positive effects of meal frequency and calorie restriction on antioxidant systems in rats. North Clin Istanbul. 2017;4(2):109–116. doi: 10.14744/nci.2017.21548.
  • Cankara FN, Özmen Ö, Savaş HB, Şahin Y, Günaydın C. Gastroprotective effect of tarantula cubensis extract in the indomethacin-induced peptic ulcer model in rats. Acta Med. Alanya 2020;4(3):278-284. doi:10.30565/medalanya.797406
  • Ralevic V, Aberdeen JA, Burnstock G. Acrylamide-induced autonomic neuropathy of rat mesenteric vessels: histological and pharmacological studies. J Auton Nerv Syst. 1991 Jun 1;34(1):77-87. doi: 10.1016/0165-1838(91)90010-z. PMID: 1940019.
  • Nurullahoğlu-Atalık E, Okudan N, Belviranlı M, Esen H, Yener Y, Oznurlu Y. Acrylamide-treatment and responses to phenylephrine and potassium in rat aorta. Acta Physiol Hung. 2012 Dec;99(4):420-9. doi: 10.1556/APhysiol.99.2012.4.6. PMID: 23238544.
  • Lin WD, Ou CC, Hsiao SH, Chang CH, Tsai FJ, Liao JW, Chen YT. Effects of Acrylamide-Induced Vasorelaxation and Neuromuscular Blockage: A Rodent Study. Toxics. 2021 May 24;9(6):117. doi: 10.3390/toxics9060117. PMID: 34074029; PMCID: PMC8225216.
  • Yu D, Xie X, Qiao B, Ge W, Gong L, Luo D, Zhang D, Li Y, Yang B, Kuang H. Gestational exposure to acrylamide inhibits mouse placental development in vivo. J Hazard Mater. 2019 Apr 5;367:160-170. doi: 10.1016/j.jhazmat.2018.12.061. Epub 2018 Dec 18. PMID: 30594716.
  • Huang M, Zhu F, Jiao J, Wang J, Zhang Y. Exposure to acrylamide disrupts cardiomyocyte interactions during ventricular morphogenesis in zebrafish embryos. Sci Total Environ. 2019 Mar 15;656:1337-1345. doi: 10.1016/j.scitotenv.2018.11.216. Epub 2018 Nov 16. PMID: 30625662.
  • El-Sayyad HI, El-Gammal HL, Habak LA, Abdel-Galil HM, Fernando A, Gaur RL, Ouhtit A. Structural and ultrastructural evidence of neurotoxic effects of fried potato chips on rat postnatal development. Nutrition. 2011 Oct;27(10):1066-75. doi: 10.1016/j.nut.2011.06.008. PMID: 21907898.
  • Zhou C, Shi Q, Liu J, Huang S, Yang C, Xiong B. Effect of Inhibiting Tumor Angiogenesis After Embolization in the Treatment of HCC with Apatinib-Loaded p(N-Isopropyl-Acrylamide-co-Butyl Methyl Acrylate) Temperature-Sensitive Nanogel. J Hepatocell Carcinoma. 2020;7:447-456. doi: 10.2147/JHC.S282209.
  • Ahmadvand D, Rahbarizadeh F, Jafari Iri-Sofla F, Namazi G, Khaleghi S, Geramizadeh B, Pasalar P, Karimi H, Aghaee Bakhtiari SH. Inhibition of angiogenesis by recombinant VEGF receptor fragments. Lab Med 2010;41:417–422
  • Li C, Shan Y, Sun Y, Si R, Liang L, Pan X, Wang B, Zhang J. Discovery of novel anti-angiogenesis agents. Part 7: Multitarget inhibitors of VEGFR-2, TIE-2 and EphB4. Eur J Med Chem. 2017;141:506-518. doi: 10.1016/j.ejmech.2017.10.030.

The Oxidative and Anti-Angiogenic Effects of Acrylamide in Chorioallantoic Membrane Model

Year 2023, Volume: 9 Issue: 2, 162 - 168, 01.05.2023

Mehmet Enes Sözen Özgür Akkaya Hasan Basri Savaş Oğuz Karahan

https://doi.org/10.53394/akd.1015590
Cited By: 2

Abstract

Objective: Acrylamide (ACR) formed spontaneously during the preparation of carbohydrate-containing foods by exposure to high heat and can be found in large amounts in processed ready-made foods like potato crisps, biscuits and crackers and bread. ACR is a toxic substance and increase oxidative stress. The aim of the study is to show the effects of acrylamide exposure at different doses on angiogenesis and oxidant-antioxidant balance in the chorioallantoic membrane model (CAM).
Methods: Two different concentrations of acrylamide is prepared (10-3 M, 10-4 M). Pellets were placed on the chorioallantoic membrane of the eggs. Before and after the experiment, liquid samples taken from the fertilized chicken eggs.
Results: ACR has an antiangiogenic effect which was dose-dependent. There is a significant increase in Total oxidant capacity (TOC) and oxidative stress index (OSI) values in 10-3 M ACR ve 10-4 M ACR groups, compared to the control group. Although there was a numerical increase in TOC and OSI values between the 10-3 M ACR and 10-4 M ACR groups, this increase was not statistically significant.
Conclusion: This is the first study that investigates the antiangiogenic effects of ACR and one of the first studies that investigates oxidative stress on the CAM model. ACR exposure increased oxidative stress and showed antiangiogenic effect in the CAM model.

Keywords

Chorioallantoic membrane model acrylamide oxidant antioxidant angiogenesis.

References

  • Semla M, Goc Z, Martiniaková M, Omelka R, Formicki G. Acrylamide: a common food toxin related to physiological functions and health. Physiol Res. 2017 May 4;66(2):205-217. doi: 10.33549/physiolres.933381. Epub 2016 Dec 16. PMID: 27982682.
  • Smith CJ, Perfetti TA, Rumple MA, Rodgman A, Doolittle DJ. "IARC group 2A Carcinogens" reported in cigarette mainstream smoke. Food Chem Toxicol. 2000 Apr;38(4):371-83. doi: 10.1016/s0278-6915(99)00156-8. PMID: 10722891.
  • Hong Y, Nan B, Wu X, Yan H, Yuan Y. Allicin alleviates acrylamide-induced oxidative stress in BRL-3A cells. Life Sci. 2019;15;231:116550. doi: 10.1016/j.lfs.2019.116550.
  • Ibrahim MA, Ibrahem MD. Acrylamide-induced hematotoxicity, oxidative stress, and DNA damage in liver, kidney, and brain of catfish (Clarias gariepinus). Environ Toxicol. 2020;35(2):300-308. doi: 10.1002/tox.22863.
  • Das AB, Srivastav PP. Acrylamide in snack foods. Toxicol Mech Methods. 2012 Apr;22(3):163-9. doi: 10.3109/15376516.2011.623329. Epub 2011 Oct 24. PMID: 22023505.
  • Santhanasabapathy R, Vasudevan S, Anupriya K, Pabitha R, Sudhandiran G. Farnesol quells oxidative stress, reactive gliosis and inflammation during acrylamide-induced neurotoxicity: Behavioral and biochemical evidence. Neuroscience. 2015 Nov 12;308:212-27. doi: 10.1016/j.neuroscience.2015.08.067. Epub 2015 Sep 1. PMID: 26341906.
  • Sumizawa T, Igisu H. Apoptosis induced by acrylamide in SH-SY5Y cells. Arch Toxicol. 2007 Apr;81(4):279-82. doi: 10.1007/s00204-006-0145-6. Epub 2006 Aug 25. PMID: 16932918.
  • Gumral N, Saygin M, Asci H, Uguz AC, Celik O, Doguc DK, Savas HB, Comlekci S. The effects of electromagnetic radiation (2450 MHz wireless devices) on the heart and blood tissue: role of melatonin. Bratisl Med J 2016;117(11):665–671. DOI: 10.4149/BLL_2016_128.
  • Savas HB, Sayar E. Oxidant Antioxidant Balance and Trace Elements In Children with Functional Dyspepsia. Turkiye Klinikleri J Med Sci. 2021;41(1):70-9. doi: 10.5336/medsci.2020-79596
  • Savas HB, Sayar E, Kara T. Thiol Disulfide Balance Oxidative Stress and Paraoxonase 1 Activities in Children and Adolescents Aged 6-16 Years with Specific Learning Disorders. Electron J Gen Med. 2021;18(3):em290. https://doi.org/10.29333/ejgm/10837
  • Savran M, Asci H, Ozmen O, Erzurumlu Y, Savas HB, Sonmez Y, Sahin Y. Melatonin protects the heart and endothelium against high fructose corn syrup consumption- induced cardiovascular toxicity via SIRT-1 signaling. Hum Exp Toxicol. 2019;38(10):1212-1223. doi: 10.1177/0960327119860188.
  • Savran M, Aslankoc R, Ozmen O, Erzurumlu Y, Savas HB, Temel EN, Kosar PA, Boztepe S. Agomelatine could prevent brain and cerebellum injury against LPS- induced neuroinflammation in rats. Cytokine. 2020;20;127:154957. doi: 10.1016/j.cyto.2019.154957.
  • Asci H, Ozmen O, Erzurumlu Y, Savas HB, Temel EN, Icten P, Hasseyid N. Ameliorative effects of pregabalin on LPS induced endothelial and cardiac toxicity. Biotech Histochem. 2020;14:1-12. doi: 10.1080/10520295.2020.1810315.
  • D. Ribatti, “The chick embryo chorioallantoic membrane (CAM) assay,” Reprod. Toxicol., vol. 70, pp. 97–101, 2017, doi: 10.1016/j.reprotox.2016.11.004.
  • Nowak-Sliwinska P, Segura T, Iruela-Arispe ML. The chicken chorioallantoic membrane model in biology, medicine and bioengineering. Angiogenesis. 2014;17(4):779-804. doi: 10.1007/s10456-014-9440-7.
  • Kadawathagedara M, Tong ACH, Heude B, Forhan A, Charles MA, Sirot V, Botton J, The Eden Mother-Child Cohort Study Group. Dietary acrylamide intake during pregnancy and anthropometry at birth in the French EDEN mother-child cohort study. Environ Res. 2016;149:189-196. doi: 10.1016/j.envres.2016.05.019. Epub 2016 May 19. PMID: 27208470.
  • Karahan O, Yavuz C, Demirtas S, Caliskan A, Atahan E. The investigation of the antiangiogenic potential of amiodarone HCl in the chick embryo chorioallantoic membrane model. Biomedical Research 2013;24:131-4.
  • Demirtas S, Caliskan A, Guclu O, Yazici S, Karahan O, Yavuz C, Mavitas B. Can calcium dobesilate be used safely for peripheral microvasculopathies that require neoangiogenesis? Med Sci Monit Basic Res. 2013;19:253-7. doi: 10.12659/MSMBR.889427.
  • Aksoy F, Aydın Bas H, A Bagcı, Savas HB. Predictive value of oxidant and antioxidant status for contrast-induced nephropathy after percutaneous coronary intervention for ST-segment elevation myocardial infarction. Rev Port Cardiol. 2020;30:S0870-2551(20)30494-7. doi: 10.1016/j.repc.2020.08.010.
  • Savas HB, Gultekin F, Ciris İM. Positive effects of meal frequency and calorie restriction on antioxidant systems in rats. North Clin Istanbul. 2017;4(2):109–116. doi: 10.14744/nci.2017.21548.
  • Cankara FN, Özmen Ö, Savaş HB, Şahin Y, Günaydın C. Gastroprotective effect of tarantula cubensis extract in the indomethacin-induced peptic ulcer model in rats. Acta Med. Alanya 2020;4(3):278-284. doi:10.30565/medalanya.797406
  • Ralevic V, Aberdeen JA, Burnstock G. Acrylamide-induced autonomic neuropathy of rat mesenteric vessels: histological and pharmacological studies. J Auton Nerv Syst. 1991 Jun 1;34(1):77-87. doi: 10.1016/0165-1838(91)90010-z. PMID: 1940019.
  • Nurullahoğlu-Atalık E, Okudan N, Belviranlı M, Esen H, Yener Y, Oznurlu Y. Acrylamide-treatment and responses to phenylephrine and potassium in rat aorta. Acta Physiol Hung. 2012 Dec;99(4):420-9. doi: 10.1556/APhysiol.99.2012.4.6. PMID: 23238544.
  • Lin WD, Ou CC, Hsiao SH, Chang CH, Tsai FJ, Liao JW, Chen YT. Effects of Acrylamide-Induced Vasorelaxation and Neuromuscular Blockage: A Rodent Study. Toxics. 2021 May 24;9(6):117. doi: 10.3390/toxics9060117. PMID: 34074029; PMCID: PMC8225216.
  • Yu D, Xie X, Qiao B, Ge W, Gong L, Luo D, Zhang D, Li Y, Yang B, Kuang H. Gestational exposure to acrylamide inhibits mouse placental development in vivo. J Hazard Mater. 2019 Apr 5;367:160-170. doi: 10.1016/j.jhazmat.2018.12.061. Epub 2018 Dec 18. PMID: 30594716.
  • Huang M, Zhu F, Jiao J, Wang J, Zhang Y. Exposure to acrylamide disrupts cardiomyocyte interactions during ventricular morphogenesis in zebrafish embryos. Sci Total Environ. 2019 Mar 15;656:1337-1345. doi: 10.1016/j.scitotenv.2018.11.216. Epub 2018 Nov 16. PMID: 30625662.
  • El-Sayyad HI, El-Gammal HL, Habak LA, Abdel-Galil HM, Fernando A, Gaur RL, Ouhtit A. Structural and ultrastructural evidence of neurotoxic effects of fried potato chips on rat postnatal development. Nutrition. 2011 Oct;27(10):1066-75. doi: 10.1016/j.nut.2011.06.008. PMID: 21907898.
  • Zhou C, Shi Q, Liu J, Huang S, Yang C, Xiong B. Effect of Inhibiting Tumor Angiogenesis After Embolization in the Treatment of HCC with Apatinib-Loaded p(N-Isopropyl-Acrylamide-co-Butyl Methyl Acrylate) Temperature-Sensitive Nanogel. J Hepatocell Carcinoma. 2020;7:447-456. doi: 10.2147/JHC.S282209.
  • Ahmadvand D, Rahbarizadeh F, Jafari Iri-Sofla F, Namazi G, Khaleghi S, Geramizadeh B, Pasalar P, Karimi H, Aghaee Bakhtiari SH. Inhibition of angiogenesis by recombinant VEGF receptor fragments. Lab Med 2010;41:417–422
  • Li C, Shan Y, Sun Y, Si R, Liang L, Pan X, Wang B, Zhang J. Discovery of novel anti-angiogenesis agents. Part 7: Multitarget inhibitors of VEGFR-2, TIE-2 and EphB4. Eur J Med Chem. 2017;141:506-518. doi: 10.1016/j.ejmech.2017.10.030.
There are 30 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Article
Authors

Mehmet Enes Sözen 0000-0002-5007-6631

Özgür Akkaya 0000-0001-6460-5066

Hasan Basri Savaş 0000-0001-8759-4507

Oğuz Karahan 0000-0003-0044-9476

Early Pub Date April 28, 2023
Publication Date May 1, 2023
Submission Date October 27, 2021
Published in Issue Year 2023 Volume: 9 Issue: 2

Cite

APA Sözen, M. E., Akkaya, Ö., Savaş, H. B., Karahan, O. (2023). The Oxidative and Anti-Angiogenic Effects of Acrylamide in Chorioallantoic Membrane Model. Akdeniz Tıp Dergisi, 9(2), 162-168. https://doi.org/10.53394/akd.1015590

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