Research Article
BibTex RIS Cite

Kınalı Keklik (Alectoris Chukar) Dalağının Morfolojik ve Histolojik Yapısının İncelenmesi

Year 2021, Volume: 16 Issue: 1, 57 - 62, 26.04.2021

Abstract

Dalak; ventriculus ve proventriculus’un birleştiği yerin sağında yer alan, bazı kan hücrelerinin depolanması, üretilmesi ve yıkımı gibi birçok görevi bulunan lenfoid bir organdır. Çalışmada kınalı keklik (Alectoris chukar)'lerde dalağın morfolojik ve histolojik özelliklerinin araştırılması amaçlanmıştır. Yapılan bu çalışmada 8 adet Kınalı keklik (Alectoris chukar) kullanıldı. Kekliklerden diseke edilen dalak dokularının morfolojik yönden incelenmek üzere ağırlığı, eni, uzunluğu, yüksekliği ölçülerek %10'luk formaldehit çözeltisinde 72 saat süreyle tespit edildi. Tespit olan bu dokular histolojik incelemeler için dehidratasyon ve şeffaflaştırma işlemlerine tabii tutuldu. Daha sonra parafin bloklara gömülerek 5μm kalınlığında kesitler alındı. Alınan kesitler Crossman’ın modifiye üçlü boyası ile boyandı ve histolojik yapısı incelendi. Morfolojik ölçümlerde ise dalağın boyunun 10.32±0.78 mm, eninin 6.60±0.50 mm, yüksekliğinin 5.55±0.26 mm, ağırlığının 0.26±0.06 gr değerleri arasında olduğu belirlendi. Kınalı keklik dalağının kalın bir kapsül ile sarılmış olduğu gözlenip, içerisinde küçük trabeküllerin bulunduğu belirlendi. Kırmızı pulpa ve beyaz pulpanın paranşim içerisinde dağınık halde olduğu, beyaz pulpa'nın retiküler hücreler, retiküler lifler ve lenfositlerden oluştuğu gözlendi. Histolojik incelemede, kırmızı pulpa'nın çok sayıda kan damarı, lenfosit, makrofaj, venöz sinüsler ile retiküler hücrelerin anastomozlarından oluştuğu belirlendi. Elde edilen veriler doğrultusunda kınalı keklik dalağının morfolojik yönden diğer kuş türleriyle farklılıklar gösterdiği bununla beraber histolojik olarak ise tavuk ve bıldırcın dalağıyla benzer olduğu gözlemlendi.

References

  • 1. Yamak US., 2015. Artificial breeding of wild birds in Turkey: Partridge breeding case. Indian J Anim Res, 49, 258-261.
  • 2. Karabag K., Alkan S., Mendes M., 2010. Classification tree method for determining factors that affecting hatchability in chukar partridge (Alectoris chukar) eggs. Kafkas Univ Vet Fak Derg, 16, 723-727.
  • 3. Nasu T., Shimizu K., Nakai M., 1992. Morphological study of the dove spleen. Poultry Sci, 71, 1527-1530.
  • 4. Rautenfeld V., Berens D., Budras KD., 1982. The bursa cloacae (Fabricii) of struthioniforms in comparison with the bursa of other birds. J Morphol, 172, 123-138.
  • 5. Kozlu T., Sari EK., Bozkurt YA., Kurtdede N., 2019. Immunohestochemical staaning of cd3, cd79αcy and s-100 on bursa fabricius, thymus and spleen of turkeys (Meleagris gallapavo). Indian J Anim Res, 53, 1450-1454.
  • 6. Powers LV., 2000. The Avian Spleen: Anatomy, Physiology and Diagnostics Compendium on Continuing Education for the Practising. Veterinarian-North American Edition. 22, 838-843.
  • 7. Islam MN., Khan MZI., Jahan MR., 2017. Shinoda K., Developmental trajectory of the prenatal lymphoid organs in native chickens: a macro anatomical study. Asian J Med Biol Res, 3, 432-436.
  • 8. Liman N., Bayram GK., 2011. Structure of the quail (Coturnix coturnix japonica) spleen during pre-and post-hatching periods. Rev Med Vet, 162, 25-33.
  • 9. Song H., Peng KSM., Li SH., Wang Y., Wei L., Tang L., 2012. Morphological characterization of the immune organs in ostrich chicks. Turk J Vet Anim Sci, 36, 89-100.
  • 10. Özüdoğru Z., Balkaya H., Kara H., Özdemir D., 2019. Morphologic and Histologic Observation of Red-Legged Partridge's (Alectoris Chukar) Liver. Van Vet J, 30, 159-161.
  • 11. Rahman ML., Islam MR., Asaduzzaman M., Khan MZI., 2003. Lymphoid tissues in the digestive tract of Deshi Chicken (Gallus domesticus) in Bangladesh. Pak J Biol Sci, 6, 1145-1150.
  • 12. Bejdic P., Avdic R., Amidzic L., Cutahija V., Tandir F., Hadziomerovic N., Mlaco N., 2018. Ultrastucture of plasma cells in harderian gland of laying hen. Anat Histol Embryol, 47, 46-50. 13. Matsuda Y., Fujii T., Suzuki T., Yamahatsu K., Kawahara K., Teduka K., Kawamoto Y., Yamamoto T., Ishiwata T., Naito Z., 2011. Comparison of fixation methods for preservation of morphology, RNAs, and proteins from paraffin-embedded human cancer cellimplanted mouse models. J Histochem Cytochem. 59, 68-75.
  • 14. Millan J., Gortazar C., Villafuerte R., 2001. Marked differences in the splanchnometry of farm-bred and wild red-legged partridges (Alectoris rufa L.). Poultry Sci, 80, 972-975.
  • 15. Kwak H., Austin RE., Dietert RR., 1999. Influence of dietary arginine concentration on lymphoid organ growthin chickens. Poultry Sci, 78, 1536- 1541.
  • 16. Puvadolpirod S., Thaxton JP., 2000. Model of physiological stress in chickens 3. Temporal patterns of response. Poultry Sci, 79, 377-382.
  • 17. Akter S., Khan MZI., Jahan MR., Karim MR., Islam MR., 2006. Histomorphological study of the lymphoid tissues of broiler chickens. Bangladesh J Vet Med, 4, 87-92.
  • 18. Sultana N., Khan MZI., Wares MA., Masum MA., 2012. Histomorphological study of the major lymphoid tissues in indigenous ducklings of Bangladesh. Bangladesh J Vet Med, 9, 53-58.
  • 19. Khan MZI., Masum M., Khan MZI., Aziz AR., Nasrin M., Siddique MNH., 2014. Histomorphology of the lymphoid tissues of broiler chickens in Kelantan, Malaysia (Histomorfologi Tisu Limfa Ayam Pedaging di Kelantan, Malaysia). Sains Malays, 43, 1175- 1179.
  • 20. Mast J., Goddeeris BM., 1997. CD57, a marker for B-cell activation and splenic ellipsoid-associated reticular cells of the chicken. Cell Tissue Res, 291, 107-115.
  • 21. Olah I., Kupper A., Ducz P., 1993. Schweigger- Seidel sheath or ellipsoid in the spleen of guinea hen. Acta Biol Hung, 45, 375-386.

Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen

Year 2021, Volume: 16 Issue: 1, 57 - 62, 26.04.2021

Abstract

The spleen of birds is located in the right side of the junction between the ventriculus and proventriculus, which is a lymphoid organ and responsible for many functions such as storing, production, and destruction of some blood cells. The aim of this study was to investigate the morphologic and histologic structures of the spleen in red-legged partridge (Alectoris chukar). In the present study, eight red-legged partridges were used. The spleens were removed from red-legged partridges and were measured in point of width, length, and weight for morphologic examination. The spleens were fixed into 10% formaldehyde solution for 72 hours. The fixed tissues were dehydrated and cleaned for histologic examinations. Then, these tissues were embedded in paraffin blocks and sectioned in a thickness of 5μm. Obtained sections were stained with Crossman Modified Triple staining and examined for their histologic structure. In the morphologic analysis, the average length of spleens was found as 10.32 ± 0.78 mm, the average width of spleens was found as 6.60 ± 0.50 mm, the average height of spleens was found as 5.55 ± 0.26 mm, and the average weight of spleens was found as 0.26 ± 0.06 g; in histologic analysis, spleen of red-legged partridge was surrounded with a thick capsule, and a few trabeculae were determined. Red and white pulps were scattered in the parenchyma; white pulps consisted of reticular cells, fibers, and lymphocytes. Red pulps consisted of macrophages, lymphocytes, venues sinuses, and reticular cells. In conclusion, morphologic features of the spleen in red-legged partridge were different from other bird species, but histologic structures were similar to chicken and quail spleens.

References

  • 1. Yamak US., 2015. Artificial breeding of wild birds in Turkey: Partridge breeding case. Indian J Anim Res, 49, 258-261.
  • 2. Karabag K., Alkan S., Mendes M., 2010. Classification tree method for determining factors that affecting hatchability in chukar partridge (Alectoris chukar) eggs. Kafkas Univ Vet Fak Derg, 16, 723-727.
  • 3. Nasu T., Shimizu K., Nakai M., 1992. Morphological study of the dove spleen. Poultry Sci, 71, 1527-1530.
  • 4. Rautenfeld V., Berens D., Budras KD., 1982. The bursa cloacae (Fabricii) of struthioniforms in comparison with the bursa of other birds. J Morphol, 172, 123-138.
  • 5. Kozlu T., Sari EK., Bozkurt YA., Kurtdede N., 2019. Immunohestochemical staaning of cd3, cd79αcy and s-100 on bursa fabricius, thymus and spleen of turkeys (Meleagris gallapavo). Indian J Anim Res, 53, 1450-1454.
  • 6. Powers LV., 2000. The Avian Spleen: Anatomy, Physiology and Diagnostics Compendium on Continuing Education for the Practising. Veterinarian-North American Edition. 22, 838-843.
  • 7. Islam MN., Khan MZI., Jahan MR., 2017. Shinoda K., Developmental trajectory of the prenatal lymphoid organs in native chickens: a macro anatomical study. Asian J Med Biol Res, 3, 432-436.
  • 8. Liman N., Bayram GK., 2011. Structure of the quail (Coturnix coturnix japonica) spleen during pre-and post-hatching periods. Rev Med Vet, 162, 25-33.
  • 9. Song H., Peng KSM., Li SH., Wang Y., Wei L., Tang L., 2012. Morphological characterization of the immune organs in ostrich chicks. Turk J Vet Anim Sci, 36, 89-100.
  • 10. Özüdoğru Z., Balkaya H., Kara H., Özdemir D., 2019. Morphologic and Histologic Observation of Red-Legged Partridge's (Alectoris Chukar) Liver. Van Vet J, 30, 159-161.
  • 11. Rahman ML., Islam MR., Asaduzzaman M., Khan MZI., 2003. Lymphoid tissues in the digestive tract of Deshi Chicken (Gallus domesticus) in Bangladesh. Pak J Biol Sci, 6, 1145-1150.
  • 12. Bejdic P., Avdic R., Amidzic L., Cutahija V., Tandir F., Hadziomerovic N., Mlaco N., 2018. Ultrastucture of plasma cells in harderian gland of laying hen. Anat Histol Embryol, 47, 46-50. 13. Matsuda Y., Fujii T., Suzuki T., Yamahatsu K., Kawahara K., Teduka K., Kawamoto Y., Yamamoto T., Ishiwata T., Naito Z., 2011. Comparison of fixation methods for preservation of morphology, RNAs, and proteins from paraffin-embedded human cancer cellimplanted mouse models. J Histochem Cytochem. 59, 68-75.
  • 14. Millan J., Gortazar C., Villafuerte R., 2001. Marked differences in the splanchnometry of farm-bred and wild red-legged partridges (Alectoris rufa L.). Poultry Sci, 80, 972-975.
  • 15. Kwak H., Austin RE., Dietert RR., 1999. Influence of dietary arginine concentration on lymphoid organ growthin chickens. Poultry Sci, 78, 1536- 1541.
  • 16. Puvadolpirod S., Thaxton JP., 2000. Model of physiological stress in chickens 3. Temporal patterns of response. Poultry Sci, 79, 377-382.
  • 17. Akter S., Khan MZI., Jahan MR., Karim MR., Islam MR., 2006. Histomorphological study of the lymphoid tissues of broiler chickens. Bangladesh J Vet Med, 4, 87-92.
  • 18. Sultana N., Khan MZI., Wares MA., Masum MA., 2012. Histomorphological study of the major lymphoid tissues in indigenous ducklings of Bangladesh. Bangladesh J Vet Med, 9, 53-58.
  • 19. Khan MZI., Masum M., Khan MZI., Aziz AR., Nasrin M., Siddique MNH., 2014. Histomorphology of the lymphoid tissues of broiler chickens in Kelantan, Malaysia (Histomorfologi Tisu Limfa Ayam Pedaging di Kelantan, Malaysia). Sains Malays, 43, 1175- 1179.
  • 20. Mast J., Goddeeris BM., 1997. CD57, a marker for B-cell activation and splenic ellipsoid-associated reticular cells of the chicken. Cell Tissue Res, 291, 107-115.
  • 21. Olah I., Kupper A., Ducz P., 1993. Schweigger- Seidel sheath or ellipsoid in the spleen of guinea hen. Acta Biol Hung, 45, 375-386.
There are 20 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Araştırma Makaleleri
Authors

Adem Kara

Derviş Özdemir 0000-0002-6038-0485

Hülya Balkaya

Hülya Kara

Zekeriya Özüdoğru

Publication Date April 26, 2021
Published in Issue Year 2021 Volume: 16 Issue: 1

Cite

APA Kara, A., Özdemir, D., Balkaya, H., Kara, H., et al. (2021). Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 16(1), 57-62.
AMA Kara A, Özdemir D, Balkaya H, Kara H, Özüdoğru Z. Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. April 2021;16(1):57-62.
Chicago Kara, Adem, Derviş Özdemir, Hülya Balkaya, Hülya Kara, and Zekeriya Özüdoğru. “Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16, no. 1 (April 2021): 57-62.
EndNote Kara A, Özdemir D, Balkaya H, Kara H, Özüdoğru Z (April 1, 2021) Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16 1 57–62.
IEEE A. Kara, D. Özdemir, H. Balkaya, H. Kara, and Z. Özüdoğru, “Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen”, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol. 16, no. 1, pp. 57–62, 2021.
ISNAD Kara, Adem et al. “Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16/1 (April 2021), 57-62.
JAMA Kara A, Özdemir D, Balkaya H, Kara H, Özüdoğru Z. Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021;16:57–62.
MLA Kara, Adem et al. “Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol. 16, no. 1, 2021, pp. 57-62.
Vancouver Kara A, Özdemir D, Balkaya H, Kara H, Özüdoğru Z. Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021;16(1):57-62.