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Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics

Year 2022, Volume: 12 Issue: 2, 418 - 422, 30.06.2022

Murat Doğan

https://doi.org/10.33808/clinexphealthsci.972758
Cited By: 1

Abstract

ABSTRACT
Objective: In this study, it was aimed to make in vitro characterization of the formulations by preparing hydrogel formulations using chitosan, a biocompatible and natural polymer, and PVP, a synthetic polymer. In addition, the effects of hydrogels containing FGF on the proliferation of keratinocyte cells were investigated.
Methods: Within the scope of the study, hydrogels with different properties were prepared and their water absorption capacity, and viscosities were examined. In addition, the hardness, adhesiveness, cohesiveness, and elasticity properties of hydrogels were investigated. The 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl tetrazolium bromide (MTT) test was applied to evaluate the toxicity of hydrogels on keratinocyte cell lines.
Results: It was observed that hydrogel formulations have high water absorption capacity and suitable viscosity values. In addition, the mechanical characterization results showed that the hydrogels have suitable mechanical properties. According to the results of in vitro cell culture studies, it has been observed that hydrogels stimulate the proliferation of keratinocyte cells.
Conclusion: Results showed that the mechanical properties of hydrogels containing FGF are suitable for application and according to the results of in vitro cell culture studies, hydrogels can be used in wound healing studies because they increase keratinocyte cell proliferation.

Keywords

Chitosan In vitro characterization Hydrogel Cell proliferation

Thanks

In this study, the facilities and devices of Cumhuriyet University Faculty of Pharmacy, Pharmaceutical Technology Department Research Laboratory and Medicine Faculty Cancer Research Center were used.

References

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  • [2] Camara FV, Ferreira LJ. Hydrogel synthesis, characterization and applications. Nova Science Publishers. Inc; 2012.
  • [3] Cretu A, Gattin R, Brachais L, Barbier-Baudry D. Synthesis and degradation of poly (2-hydroxyethyl methacrylate)-graftpoly(ε-caprolactone) copolymers. Polym Degrad Stab. 2004; 83: 399-403.
  • [4] Hajimiri M, Shahverdi S, Kamalinia G, Dinarvand R. Growth factor conjugation: Strategies and applications. J Biomed Mater Res. 2015; 103A (2): 819-838.
  • [5] Hamedi H, Moradi S, Hudson SM, Tonelli AE. Chitosan based hydrogels and their applications for drug delivery in wound dressings: A review. Carbohydr Polym. 2018; 199: 445-460.
  • [6] Huang S, Fu X. Naturally derived material-based cell and drug delivery systems in skin regeneration. J Control Release. 2010; 142: 149-159.
  • [7] Kim TH, Lee H, Park TG. Pegylated recombinant human epidermal growth factor (rh EGF) for sustained release from biodegradable PLGA microspheres. Biomaterials. 2002; 23: 2311–2317.
  • [8] Michael J, Majcher H, Todd H. Functional Biopolymers, Polymers and Polymeric Composites: A Reference Series (1st ed). Applications of Hydrogels. 2019; 453-491.
  • [9] Minoura N, Koyano T, Koshizaki N, Umehara H, Nagura M, Kobayashi K. Preparation, properties, and cell attachment/ growth behavior of PVA/chitosan-blended hydrogels. Mater Sci Eng. 1998; 6: 275-280.
  • [10] Mogoşanu DG, Grumezescu AM. Natural and synthetic polymers for wounds and burns dressing. International Journal of Pharmaceutics. 2014; 463: 127-136.
  • [11] Streubel A, Siepmann J, Bodmeier R. Gastroretentive drug delivery systems. Expert Opin. Drug Deliv. 2006; 3 (2): 217-233.
  • [12] Zhou HY, Chen XG, Kong M, Liu CS, Cha DS, Kennedy JF. Effect of molecular weight and degree of chitosan deacetylation on the preparation and characteristics of chitosan thermosensitive hydrogel as a delivery system. Carbohydr Polym. 2008; 73: 265-273.
  • [13] Andrews GP, Laverty TP, Jones DS. Mucoadhesive polymeric platforms for controlled drug delivery. Eur. J. Pharm. Biopharm. 2009; 71: 505-518.
  • [14] Wolf NB, Kuchler S, Radowski MR, Blaschke T, Kramer KD, Weindi G, Influences of opioids and nanoparticles on in vitro wound healing models. European Journal of Pharmaceutics and Biopharmaceutics. 2009; 73: 34–42.
  • [15] Singh S, Sharma B, Kanwar SS, Kumar A, Lead Phytochemicals for Anticancer Drug Development. Front. Plant Sci. 2016; 7: 8973-8985.
  • [16] Benamer S, Mahlous M, Boukrif A, Mansouri B, Youcef SL. Synthesis and characterisation of hydrogels based on poly(vinyl pyrrolidone). Nucl Instrum Methods Phys Res. 2006; 248: 284-290.
  • [17] Berger J, Reist M, Mayer JM, Fel O, Peppas NA, Gurny R. Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications, European Journal of Pharmaceutics and Biopharmaceutics. 2004; 57, 19-34.
  • [18] Cheung RCF, Bun Ng T, Wong JH, Chan WY. Chitosan: An Update on Potential Biomedical and Pharmaceutical Applications. Mar. Drugs. 2015; 13: 5156-5186.
  • [19] Croisier F, Jérôme C. Chitosan-based biomaterials for tissue engineering. Eur Polym J. 2013; 49: 780-792.
  • [20] Das N, Bera T, Mukherjee A. Biomaterial Hydrogels for Different Biomedical Applications. Int J Pharm Bio Sci. 2012; 3(3): 586-597.
  • [21] Fan L, Yang H, Yang J, Peng M, Hu J. Preparation and characterization of chitosan/gelatin/PVA hydrogel for wound dressings. Carbohydr Polym. 2016; 146: 427–434.
  • [22] Jayakumar R, Prabaharan M, Kumar TS, Nair SV. Novel Chitin and Chitosan Materials in Wound Dressing. Biomed. Eng. 2009; 1-18.
  • [23] 23. Kean T, Thanou M. Biodegradation, biodistribution and toxicity of chitosan. Adv Drug Deliv Rev. 2010; 62: 3-11.
  • [24] 24. Mohamed RR, Abu Elella MH, Sabaa MW. Synthesis, characterization and applications of N-quaternized chitosan/ poly(vinyl alcohol) hydrogels. Int J Biol Macromol. 2015; 80: 149-161

Year 2022, Volume: 12 Issue: 2, 418 - 422, 30.06.2022

Murat Doğan

https://doi.org/10.33808/clinexphealthsci.972758
Cited By: 1

Abstract

References

  • [1] Alsarra IA. Chitosan topical gel formulation in the management of burn wounds. Int J Biol Macromol. 2009; 45: 16-21
  • [2] Camara FV, Ferreira LJ. Hydrogel synthesis, characterization and applications. Nova Science Publishers. Inc; 2012.
  • [3] Cretu A, Gattin R, Brachais L, Barbier-Baudry D. Synthesis and degradation of poly (2-hydroxyethyl methacrylate)-graftpoly(ε-caprolactone) copolymers. Polym Degrad Stab. 2004; 83: 399-403.
  • [4] Hajimiri M, Shahverdi S, Kamalinia G, Dinarvand R. Growth factor conjugation: Strategies and applications. J Biomed Mater Res. 2015; 103A (2): 819-838.
  • [5] Hamedi H, Moradi S, Hudson SM, Tonelli AE. Chitosan based hydrogels and their applications for drug delivery in wound dressings: A review. Carbohydr Polym. 2018; 199: 445-460.
  • [6] Huang S, Fu X. Naturally derived material-based cell and drug delivery systems in skin regeneration. J Control Release. 2010; 142: 149-159.
  • [7] Kim TH, Lee H, Park TG. Pegylated recombinant human epidermal growth factor (rh EGF) for sustained release from biodegradable PLGA microspheres. Biomaterials. 2002; 23: 2311–2317.
  • [8] Michael J, Majcher H, Todd H. Functional Biopolymers, Polymers and Polymeric Composites: A Reference Series (1st ed). Applications of Hydrogels. 2019; 453-491.
  • [9] Minoura N, Koyano T, Koshizaki N, Umehara H, Nagura M, Kobayashi K. Preparation, properties, and cell attachment/ growth behavior of PVA/chitosan-blended hydrogels. Mater Sci Eng. 1998; 6: 275-280.
  • [10] Mogoşanu DG, Grumezescu AM. Natural and synthetic polymers for wounds and burns dressing. International Journal of Pharmaceutics. 2014; 463: 127-136.
  • [11] Streubel A, Siepmann J, Bodmeier R. Gastroretentive drug delivery systems. Expert Opin. Drug Deliv. 2006; 3 (2): 217-233.
  • [12] Zhou HY, Chen XG, Kong M, Liu CS, Cha DS, Kennedy JF. Effect of molecular weight and degree of chitosan deacetylation on the preparation and characteristics of chitosan thermosensitive hydrogel as a delivery system. Carbohydr Polym. 2008; 73: 265-273.
  • [13] Andrews GP, Laverty TP, Jones DS. Mucoadhesive polymeric platforms for controlled drug delivery. Eur. J. Pharm. Biopharm. 2009; 71: 505-518.
  • [14] Wolf NB, Kuchler S, Radowski MR, Blaschke T, Kramer KD, Weindi G, Influences of opioids and nanoparticles on in vitro wound healing models. European Journal of Pharmaceutics and Biopharmaceutics. 2009; 73: 34–42.
  • [15] Singh S, Sharma B, Kanwar SS, Kumar A, Lead Phytochemicals for Anticancer Drug Development. Front. Plant Sci. 2016; 7: 8973-8985.
  • [16] Benamer S, Mahlous M, Boukrif A, Mansouri B, Youcef SL. Synthesis and characterisation of hydrogels based on poly(vinyl pyrrolidone). Nucl Instrum Methods Phys Res. 2006; 248: 284-290.
  • [17] Berger J, Reist M, Mayer JM, Fel O, Peppas NA, Gurny R. Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications, European Journal of Pharmaceutics and Biopharmaceutics. 2004; 57, 19-34.
  • [18] Cheung RCF, Bun Ng T, Wong JH, Chan WY. Chitosan: An Update on Potential Biomedical and Pharmaceutical Applications. Mar. Drugs. 2015; 13: 5156-5186.
  • [19] Croisier F, Jérôme C. Chitosan-based biomaterials for tissue engineering. Eur Polym J. 2013; 49: 780-792.
  • [20] Das N, Bera T, Mukherjee A. Biomaterial Hydrogels for Different Biomedical Applications. Int J Pharm Bio Sci. 2012; 3(3): 586-597.
  • [21] Fan L, Yang H, Yang J, Peng M, Hu J. Preparation and characterization of chitosan/gelatin/PVA hydrogel for wound dressings. Carbohydr Polym. 2016; 146: 427–434.
  • [22] Jayakumar R, Prabaharan M, Kumar TS, Nair SV. Novel Chitin and Chitosan Materials in Wound Dressing. Biomed. Eng. 2009; 1-18.
  • [23] 23. Kean T, Thanou M. Biodegradation, biodistribution and toxicity of chitosan. Adv Drug Deliv Rev. 2010; 62: 3-11.
  • [24] 24. Mohamed RR, Abu Elella MH, Sabaa MW. Synthesis, characterization and applications of N-quaternized chitosan/ poly(vinyl alcohol) hydrogels. Int J Biol Macromol. 2015; 80: 149-161
There are 24 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Murat Doğan 0000-0003-2794-0177

Publication Date June 30, 2022
Submission Date July 17, 2021
Published in Issue Year 2022 Volume: 12 Issue: 2

Cite

APA Doğan, M. (2022). Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics. Clinical and Experimental Health Sciences, 12(2), 418-422. https://doi.org/10.33808/clinexphealthsci.972758
AMA Doğan M. Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics. Clinical and Experimental Health Sciences. June 2022;12(2):418-422. doi:10.33808/clinexphealthsci.972758
Chicago Doğan, Murat. “Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels With Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics”. Clinical and Experimental Health Sciences 12, no. 2 (June 2022): 418-22. https://doi.org/10.33808/clinexphealthsci.972758.
EndNote Doğan M (June 1, 2022) Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics. Clinical and Experimental Health Sciences 12 2 418–422.
IEEE M. Doğan, “Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics”, Clinical and Experimental Health Sciences, vol. 12, no. 2, pp. 418–422, 2022, doi: 10.33808/clinexphealthsci.972758.
ISNAD Doğan, Murat. “Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels With Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics”. Clinical and Experimental Health Sciences 12/2 (June 2022), 418-422. https://doi.org/10.33808/clinexphealthsci.972758.
JAMA Doğan M. Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics. Clinical and Experimental Health Sciences. 2022;12:418–422.
MLA Doğan, Murat. “Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels With Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics”. Clinical and Experimental Health Sciences, vol. 12, no. 2, 2022, pp. 418-22, doi:10.33808/clinexphealthsci.972758.
Vancouver Doğan M. Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics. Clinical and Experimental Health Sciences. 2022;12(2):418-22.

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