Abstract
Amaç: Bu çalışmanın amacı, resveratrolün sıçan böbreğinde cisplatin kaynaklı hasar üzerine koruyucu etkisini değerlendirmektir.
Gereç ve Yöntem: 30 adet dişi Wistar-Albino sıçan üç gruba ayrıldı: Grup 1'de (kontrol grubu) 3 gün boyunca 1 mL %0.9 NaCl (salin) intraperitoneal olarak uygulandı. Grup 2'de (cisplatin grubu) 3 gün süreyle 7.5 mg/kg intraperitoneal sisplatin verildi. Grup 3'te (cisplatin + resveratrol grubu) 7,5 mg/kg cisplatin ve 10 mg/kg resveratrol intraperitoneal olarak verildi. Sağ böbrekler tüm gruplarda cerrahi olarak ekstirpe edildi. Hem kanda hem de dokularda malondialdehit (MDA) seviyeleri ve katalaz (CAT) ve süperoksit dismutaz (SOD) aktiviteleri ölçüldü. Ayrıca böbrek dokusundan hazırlanan slaytlar ışık mikroskobu ve immünohistokimya ile incelenerek damar tıkanıklığı, kanama, tübül dejenerasyonu ve glomerüler hasar gibi toksisite belirteçleri değerlendirildi.
Bulgular: Grup 2'de doku hasarı diğer gruplara göre anlamlı derecede yüksekti. Grup 2'de diğer gruplara göre MDA düzeyleri anlamlı olarak yüksek, SOD ve CAT aktiviteleri daha düşüktü.
Sonuç: Kısa dönem bulgularımıza göre resveratrol sıçan böbreğinde cisplatinin zararlı etkisini önlemede etkili bir molekül olabilir.
Keywords
References
- 1. Fillipski KK, Loos WJ, Verweij J, Sparreboom A. Interaction of cisplatin with the human organic cation transporter 2. Clin Cancer Res 2008;14(12):3875-80.1.
- 2. R.P. Miller, R.K. Tadagavadi, G. Ramesh, W.B. Reeves. Mechanisms of cisplatin nephrotoxicity, Toxins 2010;2(11):2490-518.
- 3. Xin Yao, Kessarin Panichpisal, Neil Kurtzman, Kenneth Nugent. Cisplatin nephrotoxicity: a review. Am J Med Sci 2007;334(2):115-24.
- 4. Boulikas T, Vougiouka M. Cisplatin and platinum drugs at the molecular level (review). Oncol Rep 2003;10(6):1663-82.
- 5. Jiang M, Yi X, Hsu S, Wang CY, Dong Z. Role of p53 in cisplatin-induced tubular cell apoptosis: dependence on p53 transcriptional activity. American J Physiol 2004;287(6):1140-7.
- 6. Den Hartogh DJ, Tsiani, E. Health Benefits of Resveratrol in Kidney Disease: Evidence from In Vitro and In Vivo Studies. Nutrients 2019;11(7):1624.
- 7. Osman, AM, Bayoumi HM, AI-Harthi SE, Damanhouri ZA, Elshal MF. Modulation of doxorubicin cytotoxicity by resveratrol in a human breast cancer cell line. Cancer Cell Int 2012;12(1):47.
- 8. Mansour HH, Hafez HF, Fahmy NM. Silymarin modulates cisplatin-induced oxidative stress and hepatotoxicity in rats. J Biochem Mol Biol 2006;39(6):656-1.
- 9. Bao L, Yao XS, Tsi D. Protective effects of Bilberry (Vaccinium myrtillus L.) extract on KBrO3-induced kidney damage in mice. J Agric Food Chem 2008;56(2):420-5.
- 10. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid rection. Anal Biochem 1979;95(2):351-8.
- 11. Marklund S, Marklund G. Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47(3):469-74.
- 12. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
- 13. Dillioglugil MO, Maral Kir H, Gulkac MD, Özon Kanli A, Ozdogan HK, Acar O et al. Protective effects of increasing vitamin E and A doses on cisplatin-induced oxidative damage to kidney tissue in rats. Urol Int 2005;(4)75:340-4.
- 14. Naziroglu M, Karaoglu A, Aksoy AO. Selenium and high dose vitamin E administration protects cisplatin-induced oxidative damage to renal, liver and lens tissues in rats. Toxicol 2004;15(2):221-30.
- 15. Satoh M, Kashihara N, Fujimoto S, Horike H, Tokura T, Namikoshi T, Sasaki T, Makino H. A novel free radical scavenger, edarabone, protects against cisplatin-induced acute renal damage in vitro and in vivo. J Pharmacol Exp Ther 2003;305(3):1183-90.
- 16. Behling EB, Sendão MC, Francescato HD, Antunes LM, Costa RS, Bianchi ML. Comparative study of multiple dosage of quercetin against cisplatin-induced nephrotoxicity and oxidative stress in rat kidneys. Pharmacol Rep 2006; 58(4):526-32.
- 17. Antunes LMG, Darin JDC, Bianchi MLP. Effects of the antioxidants curcumin or selenium on cisplatin-induced nephrotoxicity and lipid peroxidation in rats. Pharmacol Res 2001;43(2):145-50.
- 18. Hascalik S, Celik O, Turkoz Y, Hascalik M, Cigremis Y, Mizrak B. Resveratrol, a red wine constituent polyphenol, protects from ischemia–reperfusion damage of the ovaries. Gynecol Obstet Invest 2004;57(4):218-23.
- 19. Gocmen AY, Burgucu D, Gumuslu S. Effect of resveratrol on platelet activation in hypercholesterolemic rats: CD40–CD40L system as a potential target. Applied Physiology, Nutrition, and Metabolism 2011;36(3): 323-30.
- 20. Singh AP, Singh R, Verma SS, Rai V, Kaschula CH, Maiti P, et al. Health benefits of resveratrol: Evidence from clinical studies. Med Res Rev 2019;39(5):1851-91.
Abstract
Purpose: The aim of this study was to evaluate the protective effect of resveratrol on cisplatin induced damage in rat kidney.
Materials and Methods: 30 female Wistar-Albino rats were allocated to form three groups: In group 1 (control group), 1 mL of 0.9% NaCl (saline) was administered intraperitoneally for 3 days. In group 2 (cisplatin group), 7.5 mg / kg intraperitoneal cisplatin was given for 3 days. In group 3 (cisplatin + resveratrol group) 7.5 mg / kg cisplatin and 10 mg / kg resveratrol were given via intraperitoneal route. Right kidneys were surgically extirpated in all groups. Malondialdehyde (MDA) levels and activities of catalase (CAT) and superoxide dismutase (SOD) were measured in both blood and tissues. Also, toxicity markers such as vascular congestion, hemorrhage, tubule degeneration and glomerular damage were assessed by examining the slides prepared from kidney tissue with microscopy.
Results: Tissue damage was significantly higher in group 2 than other groups. The MDA levels were significantly higher and the activities of SOD, and CAT were lower in group 2 than other groups.
Conclusion: According to our short term findings, resveratrol might be an effective molecule to prevent the harmful effect of cisplatin in rat kidney.
Keywords
References
- 1. Fillipski KK, Loos WJ, Verweij J, Sparreboom A. Interaction of cisplatin with the human organic cation transporter 2. Clin Cancer Res 2008;14(12):3875-80.1.
- 2. R.P. Miller, R.K. Tadagavadi, G. Ramesh, W.B. Reeves. Mechanisms of cisplatin nephrotoxicity, Toxins 2010;2(11):2490-518.
- 3. Xin Yao, Kessarin Panichpisal, Neil Kurtzman, Kenneth Nugent. Cisplatin nephrotoxicity: a review. Am J Med Sci 2007;334(2):115-24.
- 4. Boulikas T, Vougiouka M. Cisplatin and platinum drugs at the molecular level (review). Oncol Rep 2003;10(6):1663-82.
- 5. Jiang M, Yi X, Hsu S, Wang CY, Dong Z. Role of p53 in cisplatin-induced tubular cell apoptosis: dependence on p53 transcriptional activity. American J Physiol 2004;287(6):1140-7.
- 6. Den Hartogh DJ, Tsiani, E. Health Benefits of Resveratrol in Kidney Disease: Evidence from In Vitro and In Vivo Studies. Nutrients 2019;11(7):1624.
- 7. Osman, AM, Bayoumi HM, AI-Harthi SE, Damanhouri ZA, Elshal MF. Modulation of doxorubicin cytotoxicity by resveratrol in a human breast cancer cell line. Cancer Cell Int 2012;12(1):47.
- 8. Mansour HH, Hafez HF, Fahmy NM. Silymarin modulates cisplatin-induced oxidative stress and hepatotoxicity in rats. J Biochem Mol Biol 2006;39(6):656-1.
- 9. Bao L, Yao XS, Tsi D. Protective effects of Bilberry (Vaccinium myrtillus L.) extract on KBrO3-induced kidney damage in mice. J Agric Food Chem 2008;56(2):420-5.
- 10. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid rection. Anal Biochem 1979;95(2):351-8.
- 11. Marklund S, Marklund G. Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47(3):469-74.
- 12. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
- 13. Dillioglugil MO, Maral Kir H, Gulkac MD, Özon Kanli A, Ozdogan HK, Acar O et al. Protective effects of increasing vitamin E and A doses on cisplatin-induced oxidative damage to kidney tissue in rats. Urol Int 2005;(4)75:340-4.
- 14. Naziroglu M, Karaoglu A, Aksoy AO. Selenium and high dose vitamin E administration protects cisplatin-induced oxidative damage to renal, liver and lens tissues in rats. Toxicol 2004;15(2):221-30.
- 15. Satoh M, Kashihara N, Fujimoto S, Horike H, Tokura T, Namikoshi T, Sasaki T, Makino H. A novel free radical scavenger, edarabone, protects against cisplatin-induced acute renal damage in vitro and in vivo. J Pharmacol Exp Ther 2003;305(3):1183-90.
- 16. Behling EB, Sendão MC, Francescato HD, Antunes LM, Costa RS, Bianchi ML. Comparative study of multiple dosage of quercetin against cisplatin-induced nephrotoxicity and oxidative stress in rat kidneys. Pharmacol Rep 2006; 58(4):526-32.
- 17. Antunes LMG, Darin JDC, Bianchi MLP. Effects of the antioxidants curcumin or selenium on cisplatin-induced nephrotoxicity and lipid peroxidation in rats. Pharmacol Res 2001;43(2):145-50.
- 18. Hascalik S, Celik O, Turkoz Y, Hascalik M, Cigremis Y, Mizrak B. Resveratrol, a red wine constituent polyphenol, protects from ischemia–reperfusion damage of the ovaries. Gynecol Obstet Invest 2004;57(4):218-23.
- 19. Gocmen AY, Burgucu D, Gumuslu S. Effect of resveratrol on platelet activation in hypercholesterolemic rats: CD40–CD40L system as a potential target. Applied Physiology, Nutrition, and Metabolism 2011;36(3): 323-30.
- 20. Singh AP, Singh R, Verma SS, Rai V, Kaschula CH, Maiti P, et al. Health benefits of resveratrol: Evidence from clinical studies. Med Res Rev 2019;39(5):1851-91.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research |
| Authors | |
| Publication Date | September 30, 2022 |
| Acceptance Date | July 17, 2022 |
| Published in Issue | Year 2022 Volume: 47 Issue: 3 |
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