Research Article
Year 2019,
Volume: 46 Issue: 2, 269 - 276, 11.06.2019
Abstract
Objective:
To find out the prevalence of thalassemias and various other hemoglobinopathies
in state of Haryana and to assess the role of High performance liquid
chromatography (HPLC) for accurate and quick diagnosis of various hemoglobin
disorders.
Methods:
This study was conducted in Pt. B. D. Sharma, PGIMS, Rohtak from July 2010 to
October 2016 for hemoglobin variant screening by HPLC of total of 4275
patients, selected through multistage random sampling technique.
Results:
Among 4275 total cases surveyed, normal hemoglobin pattern was observed in 3862
(90.3%) and abnormal hemoglobin fractions on HPLC were detected in 413 (9.7%).
Βeta (β) thalassemia trait was predominant abnormality found in a total of 318
cases (7.4%). There were 19 cases of β-thalassemia major and 24 of
β-thalassemia intermedia. Other abnormal patterns found were HbE (trait in 11
and HbE disease in 6 cases), Hb S (2 cases each of sickle cell disease and
sickle cell trait), 2 cases of high Hb F (1 case each of heterozygous and
homozygous HPFH), 11 cases of Hb D Punjab along with 2 cases of Hb D Iran, 1
case of Hb C and 2 cases each of double heterozygotes for Hb E/ β-thalassemia
and Hb S/ β-thalassemia.
Conclusion:
There is high prevalence of hemoglobinopathies in this region and HPLC forms a
rapid and accurate tool in early detection and management of various
hemoglobinopathies.
Keywords
References
- 1. Edward J. Benz Jr. Disorders of Hemoglobin Chapter 104, in Harrision’s Principle of Internal Medicine, 18th edition, 2011; 1: 852-61.
- 2. Tyagi S, Saxena R, Choudhry VP. HPLC--how necessary is it forhaemoglobinopathy diagnosis in India? Indian J Pathol Microbiol 2003; 46: 390-3.
- 3. WHO- EXECUTIVE BOARD EB118/5, 118th Session Report by the Secretariat on Thalassemia and other hemoglobinopathies: Prevalence of Hemoglobinopathies. 11 May 2006,p1-8.
- 4. Balgir RS. The genetic burden of hemoglobinopathies with special reference to community health in India and the challenges ahead. Indian Journal of Hematology and Blood Transfusion. 2002; 20: 2-7.
- 5. Joutovsky A, Hadzi-Nesic J, Nardi M. HPLC retention time as diagnostic tool for hemoglobin variants and hemoglobinopathies: A study of 60,000 samples in clinical diagnostic laboratory. Clinical chemistry. 2004; 50: 1736-47.
- 6. Alamiry AAN, Ali TH, Majeed MN. Detection of hemoglobinopathies in hypochromic, microcytic and sickeled cell blood films by haemoglobin electrophoresis. Thi-Qar medical journal (TQMJ): 2011; 5: 139-48.
- 7. Bravo-Urquiola M, Arends A, Montilla S, et all. Advantages in the use of high performance chromatography technique for screening hemoglobinopathies in Venezuela. Invest Clin2004; 45: 309-15.
- 8. Verma P, Ghildiyal A, Verma D, et all. Prevalence of hemoglobinopathies in different regions and castes of Uttar Pradesh, India- A hospital based study. Asian J Med Sci 2015; 6: 21-5.
- 9. Mondal SK, Dasgupta S, Mondal S, Das N. Spectrum of thalassemias and hemoglobinopathies in West Bengal: A study of 90,210 cases by cation exchange high performance liquid chromatography method over a period of 8 years. J Applied Hemat 2014; 5: 91-5.
- 10. Jain BB, Roy RN, Ghosh S, et all. Screening for thalassemia and other hemoglobinopathies in tertiary care hospital of West Bengal: Implications for population screening. Indian J Public Health 2012; 56: 297-300.
- 11. Patel U, Shrivastav A, Joshi JR, et all. Detection of hemoglobinopathies and thalassemias in population of Gujarat State using HPLC: analysis of 2022 cases. Pathology and laboratory medicine 2012; 4: 80-4.
- 12. Madan N, Sharma S, Sood SK, Colah R, Bhatia LH. Frequency of Beta thalassemia trait and other hemoglobinopathies in northern and western India. Indian J Hum Genet 2010; 16: 16-25.
- 13. Sachdev R, Dam AR, Tyagi G. Detection of Hb variants and hemoglobinopathies in indian population using HPLC: report of 2600 cases. Indian J Path Micro 2010; 53: 57-62.
- 14. Bhalodia JN, Oza HV, Modi PJ, et all. Study of hemoglobinopathies in patients of anemia using high performance liqiuid chromatography (HPLC) in western India. Nat J Community Med 2015; 6: 35-40.
- 15. Mondal SK, Mandal S. Prevalence of thalassemia and hemoglobinopathies in eastern India: A 10 year high performance liquid chromatography study of 119,336 cases. Asian J Transfs Sci 2016; 10: 105-10.
- 16. Haritha P, Lakshmi V, Veerraju P, Sarkar BN, RaoVR. Prevalence of hemoglobinopathies among Konda Kammaras of Visakhapatnam district, Andhra Pradesh. J Pharm Bio Sci 2012; 2: 06-8.
- 17. Ghosh N, Chakrabarti I, Chakraborty M, Goswami BK. A community based pilot study on prevalence of hemoglobinopathies among the antenatal women in a rural area of Darjeeling district, West Bengal. Inter J Med and Pub Health 2013; 3: 107-10.
Year 2019,
Volume: 46 Issue: 2, 269 - 276, 11.06.2019
Abstract
References
- 1. Edward J. Benz Jr. Disorders of Hemoglobin Chapter 104, in Harrision’s Principle of Internal Medicine, 18th edition, 2011; 1: 852-61.
- 2. Tyagi S, Saxena R, Choudhry VP. HPLC--how necessary is it forhaemoglobinopathy diagnosis in India? Indian J Pathol Microbiol 2003; 46: 390-3.
- 3. WHO- EXECUTIVE BOARD EB118/5, 118th Session Report by the Secretariat on Thalassemia and other hemoglobinopathies: Prevalence of Hemoglobinopathies. 11 May 2006,p1-8.
- 4. Balgir RS. The genetic burden of hemoglobinopathies with special reference to community health in India and the challenges ahead. Indian Journal of Hematology and Blood Transfusion. 2002; 20: 2-7.
- 5. Joutovsky A, Hadzi-Nesic J, Nardi M. HPLC retention time as diagnostic tool for hemoglobin variants and hemoglobinopathies: A study of 60,000 samples in clinical diagnostic laboratory. Clinical chemistry. 2004; 50: 1736-47.
- 6. Alamiry AAN, Ali TH, Majeed MN. Detection of hemoglobinopathies in hypochromic, microcytic and sickeled cell blood films by haemoglobin electrophoresis. Thi-Qar medical journal (TQMJ): 2011; 5: 139-48.
- 7. Bravo-Urquiola M, Arends A, Montilla S, et all. Advantages in the use of high performance chromatography technique for screening hemoglobinopathies in Venezuela. Invest Clin2004; 45: 309-15.
- 8. Verma P, Ghildiyal A, Verma D, et all. Prevalence of hemoglobinopathies in different regions and castes of Uttar Pradesh, India- A hospital based study. Asian J Med Sci 2015; 6: 21-5.
- 9. Mondal SK, Dasgupta S, Mondal S, Das N. Spectrum of thalassemias and hemoglobinopathies in West Bengal: A study of 90,210 cases by cation exchange high performance liquid chromatography method over a period of 8 years. J Applied Hemat 2014; 5: 91-5.
- 10. Jain BB, Roy RN, Ghosh S, et all. Screening for thalassemia and other hemoglobinopathies in tertiary care hospital of West Bengal: Implications for population screening. Indian J Public Health 2012; 56: 297-300.
- 11. Patel U, Shrivastav A, Joshi JR, et all. Detection of hemoglobinopathies and thalassemias in population of Gujarat State using HPLC: analysis of 2022 cases. Pathology and laboratory medicine 2012; 4: 80-4.
- 12. Madan N, Sharma S, Sood SK, Colah R, Bhatia LH. Frequency of Beta thalassemia trait and other hemoglobinopathies in northern and western India. Indian J Hum Genet 2010; 16: 16-25.
- 13. Sachdev R, Dam AR, Tyagi G. Detection of Hb variants and hemoglobinopathies in indian population using HPLC: report of 2600 cases. Indian J Path Micro 2010; 53: 57-62.
- 14. Bhalodia JN, Oza HV, Modi PJ, et all. Study of hemoglobinopathies in patients of anemia using high performance liqiuid chromatography (HPLC) in western India. Nat J Community Med 2015; 6: 35-40.
- 15. Mondal SK, Mandal S. Prevalence of thalassemia and hemoglobinopathies in eastern India: A 10 year high performance liquid chromatography study of 119,336 cases. Asian J Transfs Sci 2016; 10: 105-10.
- 16. Haritha P, Lakshmi V, Veerraju P, Sarkar BN, RaoVR. Prevalence of hemoglobinopathies among Konda Kammaras of Visakhapatnam district, Andhra Pradesh. J Pharm Bio Sci 2012; 2: 06-8.
- 17. Ghosh N, Chakrabarti I, Chakraborty M, Goswami BK. A community based pilot study on prevalence of hemoglobinopathies among the antenatal women in a rural area of Darjeeling district, West Bengal. Inter J Med and Pub Health 2013; 3: 107-10.
There are 17 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Original Articles |
| Authors | |
| Publication Date | June 11, 2019 |
| Submission Date | September 8, 2017 |
| Published in Issue | Year 2019 Volume: 46 Issue: 2 |