Research Article
Year 2020,
Volume: 3 Issue: 3, 111 - 116, 31.12.2020
Abstract
AMAÇ: Stronsiyum (Sr), osteoporoz tedavisinde uzun yıllardır kullanılan bir ilaçtır. Son dönemlerde Sr’nin pro-inflamatuvar sitokinleri inhibe ederek anti-inflamatuvar etkiler yaptığı gösterilmiştir. Bu çalışmanın amacı, Sr kloridin anti-inflamatuvar etkilerini ratlarda Freund’s Complete Adjuvan (FCA) kullanılarak oluşturulan deneysel artrit modelinde klinik, histopatolojik, immunohistokimyasal TNF-α ve TNF-α, IL-1 β, IL-6 serum seviyeleri ile değerlendirmektir.
YÖNTEM: Çalışmaya 48 adet wistar-albino cinsi dişi rat alınmıştır. Ratlar randomize olarak her grupta 8 rat olacak biçimde toplam 6 gruba ayrıldı. Grup I kontrol grubudur, diğer gruplarda deneysel artrit modeli oluşturulmuştur. Grup II artrit kontrol olarak ayrılmıştır. Grup III, Grup IV ve Grup V’e Sr klorid oral olarak verilmiştir. Grup VI’ya diklofenak sodyum oral olarak verilmiştir. Deney sürecinde aralıklı olarak grupların ağırlık ölçümleri, sağ pençe çevresi ölçümleri ve klinik artrit skorları takip edilmiştir. Deney sonunda deneklerden alınan kan örneklerinden serum TNF-α, IL-1 β, IL-6 çalışılmıştır. Alınan doku örneklerinden histopatolojik olarak inflamasyon skoru, sinovyal hiperplazi, kıkırdak erozyonu ve kemik erozyonu değerlendirilmiştir. Alınan doku örneklerinden immunohistokimyasal TNF-α değerlendirilmiştir.
BULGULAR: Sr kloridin anti-inflamatuvar etkileri değerlendirmelerde gösterilmiştir. Serum örneklerinden çalışılan TNF-α, IL-1 β, IL-6 seviyelerinde gruplar arasında anlamlı farklılık yoktu. İmmunohistokimyasal TNF-α değerlendirmesinde kontrol grubu hariç diğer gruplarda anlamlı farklılık yoktu.
SONUÇ: Çalışmamızda Sr kloridin anti-inflamatuvar etkileri klinik ve histopatolojik değerlendirmeler ile gösterilmiştir. Bu sonuçlar Romatoid Artrit tedavisinde Sr kloridin kullanılabileceğini düşündürmektedir.
Supporting Institution
Cumhuriyet Üniversitesi Bilimsel Araştırma Projeleri tarafından 2018, T-780 numaralı proje ile desteklenmiştir.
Project Number
T-780
Thanks
Cumhuriyet Üniversitesi Bilimsel Araştırma Projeleri'ne çalışmaıza verdikleri destek için teşekkür ederiz.
References
- Weissmann G. The pathogenesis of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2006;64(1-2):12-15.
- Ergin S. Romatoid Artrit. In: Beyazova, M; Gokce Kutsal Y (ed). Fiziksel Tıp ve Rehabilitasyon. 2. ed. Ankara: Güneş Tıp Kitapevleri; 2011: 2199-2220.
- Gravallese, EM; Monach P. Pathogenesis and pathology of rheumatoid arthritis. In: Hochberg MC, editor. Rheumatology (Hochberg). seventh ed. Elsevier Ltd; 2019:811-31.
- Chen G. Immunotherapy of rheumatoid arthritis targeting inflammatory cytokines and autoreactive T cells. Arch Immunol Ther Exp (Warsz). 2010;58(1):27-36.
- Burska A, Boissinot M, Ponchel F. Cytokines as Biomarkers in Rheumatoid Arthritis. Mediators Inflamm. 2014;2014:1-24.
- Burmester GR, Pope JE. Novel treatment strategies in rheumatoid arthritis. Lancet. 2017;389(10086):2338–48.
- Boehncke, A; Koennecker, G; Mangelsdorf, I; Wibbertmann A. Concise international chemical assessment document 6. IPCS Concise Int Chem Assess Doc. 1999;(6):1–37.
- Reginster JY, Seeman E, De Vernejoul MC, Adami S, Compston J, Phenekos C, et al. Strontium ranelate reduces the risk of nonvertebral fractures in postmenopausal women with osteoporosis: Treatment of Peripheral Osteoporosis (TROPOS) study. J Clin Endocrinol Metab. 2005;90(5):2816–22.
- Rizzoli R, Chapurlat RD, Laroche JM, Krieg MA, Thomas T, Frieling I, et al. Effects of strontium ranelate and alendronate on bone microstructure in women with osteoporosis results of a 2-year study. Osteoporos Int. 2012;23(1):305–15.
- de Melo Nunes R, Martins MR, da Silva Junior FS, de Melo Leite ACR, Girão VCC, de Queiroz Cunha F, et al. Strontium ranelate analgesia in arthritis models is associated to decreased cytokine release and opioid-dependent mechanisms. Inflamm Res. 2015;64(10):781–7.
- Zhu S, Hu X, Tao Y, Ping Z, Wang L, Shi J, et al. Strontium inhibits titanium particle-induced osteoclast activation and chronic inflammation via suppression of NF-κB pathway. Sci Rep. 2016;6:1–11.
- Pelletier JP, Kapoor M, Fahmi H, Lajeunesse D, Blesius A, Maillet J, et al. Strontium ranelate reduces the progression of experimental dog osteoarthritis by inhibiting the expression of key proteases in cartilage and of IL-1β in the synovium. Ann Rheum Dis. 2013;72(2):250–7.
- Lafeber FPJG, van Laar JM. Strontium ranelate: ready for clinical use as disease-modifying osteoarthritis drug? Ann Rheum Dis. 2013;72(2):157–61.
- Rodrigues TA, Freire AO, Bonfim BF, Cartágenes MSS, Garcia JBS. Strontium ranelate as a possible disease-modifying osteoarthritis drug: a systematic review. Brazilian J Med Biol Res. 2018;51(8):1-9.
- Reginster JY, Beaudart C, Neuprez A, Bruyère O. Strontium ranelate in the treatment of knee osteoarthritis: New insights and emerging clinical evidence. Ther Adv Musculoskelet Dis. 2013;5(5):268–76.
- Kumar VL, Guruprasad B, Wahane VD. Atorvastatin exhibits anti-inflammatory and anti-oxidant properties in adjuvant-induced monoarthritis. Inflammopharmacology. 2010;18(6):303–8.
- Talwar S, Nandakumar K, Nayak PG, Bansal P, Mudgal J, Mor V, et al. Anti-inflammatory activity of Terminalia paniculata bark extract against acute and chronic inflammation in rats. J Ethnopharmacol. 2011;134(2):323–8.
- Trentham BYDE, Townes AS, Kang AH. Autoimmunity to type II collagen: An experimental model of arthritis. 1978;146:10–1.
- Ferro F, Elefante E, Luciano N, Talarico R, Todoerti M, Elefante E, et al. Review One year in review 2017 : novelties in the treatment of rheumatoid arthritis. Clin Exp Rheumatol. 2017;35:721–34.
- Buache E, Velard F, Bauden E, Guillaume C, Jallot E, Nedelec JM, et al. Effect of strontium-substituted biphasic calcium phosphate on inflammatory mediators production by human monocytes. Acta Biomater. 2012;8(8):3113–9.
- Topal F, Yonem O, Tuzcu N, Tuzcu M, Ataseven H, Akyol M. Strontium chloride: Can it be a new treatment option for ulcerative colitis? Biomed Res Int. 2014;2014.
- Liu X, Zhu S, Cui J, Shao H, Zhang W, Yang H, et al. Strontium ranelate inhibits titanium-particle-induced osteolysis by restraining inflammatory osteoclastogenesis in vivo. Acta Biomater. 2014;10(11):4912–8.
- Mierzwa AGH, Campos JF, Jesus MF, Nader HB, Lazaretti-Castro M, Reginato RD. Different doses of strontium ranelate and mechanical vibration modulate distinct responses in the articular cartilage of ovariectomized rats. Osteoarthr Cartil. 2017;25(7):1179–88.
Year 2020,
Volume: 3 Issue: 3, 111 - 116, 31.12.2020
Abstract
Project Number
T-780
References
- Weissmann G. The pathogenesis of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2006;64(1-2):12-15.
- Ergin S. Romatoid Artrit. In: Beyazova, M; Gokce Kutsal Y (ed). Fiziksel Tıp ve Rehabilitasyon. 2. ed. Ankara: Güneş Tıp Kitapevleri; 2011: 2199-2220.
- Gravallese, EM; Monach P. Pathogenesis and pathology of rheumatoid arthritis. In: Hochberg MC, editor. Rheumatology (Hochberg). seventh ed. Elsevier Ltd; 2019:811-31.
- Chen G. Immunotherapy of rheumatoid arthritis targeting inflammatory cytokines and autoreactive T cells. Arch Immunol Ther Exp (Warsz). 2010;58(1):27-36.
- Burska A, Boissinot M, Ponchel F. Cytokines as Biomarkers in Rheumatoid Arthritis. Mediators Inflamm. 2014;2014:1-24.
- Burmester GR, Pope JE. Novel treatment strategies in rheumatoid arthritis. Lancet. 2017;389(10086):2338–48.
- Boehncke, A; Koennecker, G; Mangelsdorf, I; Wibbertmann A. Concise international chemical assessment document 6. IPCS Concise Int Chem Assess Doc. 1999;(6):1–37.
- Reginster JY, Seeman E, De Vernejoul MC, Adami S, Compston J, Phenekos C, et al. Strontium ranelate reduces the risk of nonvertebral fractures in postmenopausal women with osteoporosis: Treatment of Peripheral Osteoporosis (TROPOS) study. J Clin Endocrinol Metab. 2005;90(5):2816–22.
- Rizzoli R, Chapurlat RD, Laroche JM, Krieg MA, Thomas T, Frieling I, et al. Effects of strontium ranelate and alendronate on bone microstructure in women with osteoporosis results of a 2-year study. Osteoporos Int. 2012;23(1):305–15.
- de Melo Nunes R, Martins MR, da Silva Junior FS, de Melo Leite ACR, Girão VCC, de Queiroz Cunha F, et al. Strontium ranelate analgesia in arthritis models is associated to decreased cytokine release and opioid-dependent mechanisms. Inflamm Res. 2015;64(10):781–7.
- Zhu S, Hu X, Tao Y, Ping Z, Wang L, Shi J, et al. Strontium inhibits titanium particle-induced osteoclast activation and chronic inflammation via suppression of NF-κB pathway. Sci Rep. 2016;6:1–11.
- Pelletier JP, Kapoor M, Fahmi H, Lajeunesse D, Blesius A, Maillet J, et al. Strontium ranelate reduces the progression of experimental dog osteoarthritis by inhibiting the expression of key proteases in cartilage and of IL-1β in the synovium. Ann Rheum Dis. 2013;72(2):250–7.
- Lafeber FPJG, van Laar JM. Strontium ranelate: ready for clinical use as disease-modifying osteoarthritis drug? Ann Rheum Dis. 2013;72(2):157–61.
- Rodrigues TA, Freire AO, Bonfim BF, Cartágenes MSS, Garcia JBS. Strontium ranelate as a possible disease-modifying osteoarthritis drug: a systematic review. Brazilian J Med Biol Res. 2018;51(8):1-9.
- Reginster JY, Beaudart C, Neuprez A, Bruyère O. Strontium ranelate in the treatment of knee osteoarthritis: New insights and emerging clinical evidence. Ther Adv Musculoskelet Dis. 2013;5(5):268–76.
- Kumar VL, Guruprasad B, Wahane VD. Atorvastatin exhibits anti-inflammatory and anti-oxidant properties in adjuvant-induced monoarthritis. Inflammopharmacology. 2010;18(6):303–8.
- Talwar S, Nandakumar K, Nayak PG, Bansal P, Mudgal J, Mor V, et al. Anti-inflammatory activity of Terminalia paniculata bark extract against acute and chronic inflammation in rats. J Ethnopharmacol. 2011;134(2):323–8.
- Trentham BYDE, Townes AS, Kang AH. Autoimmunity to type II collagen: An experimental model of arthritis. 1978;146:10–1.
- Ferro F, Elefante E, Luciano N, Talarico R, Todoerti M, Elefante E, et al. Review One year in review 2017 : novelties in the treatment of rheumatoid arthritis. Clin Exp Rheumatol. 2017;35:721–34.
- Buache E, Velard F, Bauden E, Guillaume C, Jallot E, Nedelec JM, et al. Effect of strontium-substituted biphasic calcium phosphate on inflammatory mediators production by human monocytes. Acta Biomater. 2012;8(8):3113–9.
- Topal F, Yonem O, Tuzcu N, Tuzcu M, Ataseven H, Akyol M. Strontium chloride: Can it be a new treatment option for ulcerative colitis? Biomed Res Int. 2014;2014.
- Liu X, Zhu S, Cui J, Shao H, Zhang W, Yang H, et al. Strontium ranelate inhibits titanium-particle-induced osteolysis by restraining inflammatory osteoclastogenesis in vivo. Acta Biomater. 2014;10(11):4912–8.
- Mierzwa AGH, Campos JF, Jesus MF, Nader HB, Lazaretti-Castro M, Reginato RD. Different doses of strontium ranelate and mechanical vibration modulate distinct responses in the articular cartilage of ovariectomized rats. Osteoarthr Cartil. 2017;25(7):1179–88.
There are 23 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Internal Diseases |
| Journal Section | Original Investigation |
| Authors | |
| Project Number | T-780 |
| Publication Date | December 31, 2020 |
| Acceptance Date | December 3, 2020 |
| Published in Issue | Year 2020 Volume: 3 Issue: 3 |
