Examination of Oxidative Stress Level in Adolescents with Polycystic Ovary Syndrome by Biochemical Parameters
Abstract
Polycystic ovary syndrome (PCOS) is one of the common endocrine disorders among women. Its prevalence increases up to 15% based on the Rotterdam criteria. It has been extensively studied for over 10 years that, among many factors, oxidative stress (OS) is a potential factor in the etiology of PCOS. This study aims to investigate the role of OS in the pathogenesis of PCOS. An examination was made on 30 patients with PCOS and 30 healthy adolescent and young adult women aged between 15-22 years. The diagnosis of PCOS was established considering the recommendations of the last Amsterdam ESHRE/ASRM conference. Basal hormone levels in the early follicular phase, fasting glucose and insulin values, serum lipid profiles, serum albumin, ischemia-modified albumin (IMA), total thiol, native thiol, and disulfide levels were recorded as the patients' main parameters. Albumin, IMA, thiol, and disulfide levels were compared between the PCOS and control groups. The albumin level was statistically significantly higher in the PCOS group than in the control group. A negative correlation was detected between serum CRP levels and serum albumin, native thiol, and total thiol levels. Total and native thiol values were statistically significantly lower in the BMI overweight group. In conclusion, in the present study in which OS markers in 30 PCOS patients and 30 healthy adolescent and young adult women were examined, the serum albumin level was statistically significantly higher in the PCOS group compared to the control group, and IMA, total and native thiol and disulfide levels did not differ between the groups. For a clear understanding of the place of OS in the diagnosis of PCOS, it should be supported by studies involving larger patient groups and adult women of reproductive age.
References
- Fauser, B.C, Tarlatzis, B.C, Rebar, R.W. et al. (2012). “Consensus on Women's Health Aspects of Polycystic Ovary Syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group, 97(1), 28-38
- Hart, R. and Doherty, D.A. (2015). “The Potential Implications of A PCOS Diagnosis on A Woman's Long-Term Health Using Data Linkage. The Journal of Clinical Endocrinology and Metabolism, 100 (3), 911-9.
- Peña, A.S, Witchel, S.F, Hoeger, K.M. et al. (2020). “Adolescent Polycystic Ovary Syndrome According To The International Evidence-Based Guideline”. BMC Medicine, 18 (1), 72.
- Ajmal, N, Khan, S.Z. and Shaikh, R. (2019). “Polycystic Ovary Syndrome (PCOS) and Genetic Predisposition: A Review Article”. European Journal Of Obstetrics & Gynecology and Reproductive Biology, X, 3, 100060.
- Barber, T.M, Dimitriadis, G.K, Andreou, A, et al. (2015). “Polycystic Ovary Syndrome: Insight Into Pathogenesis and A Common Association With Insulin Resistance”. Clin Med (Lond), 15 (6), 72-76.
- Repaci, A, Gambineri, A. and Pasquali, R. (2011). “The Role of Low-Grade Inflammation in The Polycystic Ovary Syndrome”. Mol Cell Endocrinol. 335 (1), 30-41.
- Visser, J.A. (2021). “The Importance of Metabolic Dysfunction in Polycystic Ovary Syndrome”. Nature reviews Endocrinology, 17 (2), 77-78.
- McCord, J.M. (1993). “Human Disease, Free Radicals, and The Oxidant/Antioxidant Balance.” Clin Biochem, 26 (5), 351-357.
- Gergerlioglu, H.S, Savas, H.A, Bulbul, F. et al. (2007). “Changes in Nitric Oxide Level and Superoxide Dismutase Activity during Antimanic Treatment”. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 31 (3), 697-702.
- Macut, D, Bjekic-Macut, J. and Savic-Radojevic, A. (2013). “Dyslipidemia and Oxidative Stress in PCOS”. Front Horm Res, 40, 51-63.
- Bar-Or, D, Rael, L.T, Bar-Or, R. et al. (2008). “The Cobalt-Albumin Binding Assay: Insights into Its Mode of Action”. Clin Chim Acta, 387 (1-2), 120-127.
- Ustun, Y, Engin-Ustun, Y, Ozturk, O. et al. (2011). “Ischemia-Modified Albumin As An Oxidative Stress Marker In Preeclampsia”. The journal of Maternal-Fetal & Neonatal Medicine: The Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstet, 24 (3), 418-21.
- Ukinc, K, Eminagaoglu, S, Ersoz, H.O. et al. (2009). “A Novel Indicator of Widespread Endothelial Damage and Ischemia in Diabetic Patients: Ischemia-Modified Albumin”. Endocrine, 36 (3), 425-32.
- Seshadri, Reddy, V, Bukke, S. and Munikumar, M. (2018). “Elevated Levels of The Circulatory Ischemia-Modified Albumin in Patients with Polycystic Ovary Syndrome: A Meta-Analysis.” Gynecol Endocrinol. 34 (10), 868-874.
- Erel, O. and Neselioglu, S. (2014). “A Novel and Automated Assay for Thiol/Disulphide Homeostasis”. Clin Biochem. 47 (18), 326-32.
- Dean, R.T, Fu, S, Stocker, R. et al. (1997). “Biochemistry and Pathology of Radical-Mediated Protein Oxidation.” The Biochemical Journal, 324 (1), 1-18.
- Murri, M, Luque-Ramirez, M, Insenser, M. et al. (2013). “Circulating Markers of Oxidative Stress and Polycystic Ovary Syndrome (PCOS): A Systematic Review and Meta-Analysis”. Hum Reprod Update, 19 (3), 268-88.
- Kanafchian, M, Esmaeilzadeh, S, Mahjoub, S, et al. (2020). “Status of Serum Copper, Magnesium, and Total Antioxidant Capacity in Patients with Polycystic Ovary Syndrome.” Biological Trace Element Research, 193 (1), 111-117.
- Turan, V, Sezer, E.D, Zeybek, B, et al. (2015). “Infertility and The Presence of Insulin Resistance Are Associated with Increased Oxidative Stress in Young, Non-Obese Turkish Women with Polycystic Ovary Syndrome”. Journal of Pediatric and Adolescent Gynecology, 28 (2), 119-23.
- Nasiri, N, Moini, A, Eftekhari-Yazdi, P. et al. (2015). “Abdominal Obesity Can Induce both Systemic and Follicular Fluid Oxidative Stress Independent from Polycystic Ovary Syndrome”. Eur J Obstet Gynecol Reprod Biol, 184, 112-126.
- Suresh, S. and Vijayakumar, T. (2015). “Correlations of Insulin Resistance and Serum Testosterone Levels with LH:FSH Ratio and Oxidative Stress in Women with Functional Ovarian Hyperandrogenism.” Indian Journal of Clinical Biochemistry : IJCBİ, 30 (3), 345-50.
- Ozler, S, Oztas, E. and Tokmak, A. et al. (2016). “The Association of thiol/disulphide Homeostasis and Lipid Accumulation Index with Cardiovascular Risk Factors in Overweight Adolescents with Polycystic Ovary Syndrome”. Clin Endocrinol (Oxf), 84 (4), 516-23.
- Baskol, G, Aygen, E, Erdem, F. et al. (2012). “Assessment of Paraoxonase 1, Xanthine Oxidase and Glutathione Peroxidase Activities, Nitric Oxide and Thiol Levels in Women with Polycystic Ovary Syndrome”. Acta Obstet Gynecol Scand, 91 (3), 326-30.
- Beyazit, F, Yilmaz, N, Balci, O. et al. (2016). “Evaluation of Oxidative Stress in Women with Polycystic Ovarian Syndrome as Represented by Serum Ischemia Modified Albumin and Its Correlation with Testosterone and Insulin Resistance”. Internal Medicine (Tokyo, Japan), 55 (17), 2359-64.
- Samy, N, Hashim, M, Sayed, M, et al. (2009). “Clinical Significance of Inflammatory Markers in Polycystic Ovary Syndrome: Their Relationship to Insulin Resistance and Body Mass Index”. Disease Markers, 26 (4), 163-70.
- Caglar, G.S, Oztas, E, Karadag, D. et al. (2011). “Ischemia-Modified Albumin and Cardiovascular Risk Markers in Polycystic Ovary Syndrome with or Without Insulin Resistance.” Fertility and Sterility, 95 (1), 310-313.
- Cakir, E, Ozbek, M, Ozkaya, E. et al. (2011). “Oxidative Stress Markers are Not Valuable Markers in Lean and Early Age of Polycystic Ovary Syndrome Patients.” J Endocrinol Invest, 34 (7), e178-82.
- Franks, S, Gharani, N, Waterworth, D. et al. (1997). “The Genetic Basis of Polycystic Ovary Syndrome.” Hum Reprod, 12 (12), 2641-2648.
- Shang, Y, Zhou, H, Hu, M, et al. (2020). “Effect of Diet on Insulin Resistance in Polycystic Ovary Syndrome”. The Journal of Clinical Endocrinology and Metabolism, 105 (10), 425.
Polikistik Over Sendromlu Adolesanlarda Oksidatif Stres Düzeyinin Biyokimyasal Parametrelerle İncelenmesi
Abstract
Polikistik over sendromu (PCOS), kadınlar arasında görülen yaygın endokrin bozukluklardan biridir. Prevalansı Rotterdam kriterleri baz alındığında %15’e kadar çıkmaktadır. Birçok etken arasında oksidatif stresin (OS), PCOS etiyolojisinde yer alan potansiyel bir faktör olduğu 10 yılı aşkın bir süredir yoğun bir şekilde çalışılmaktadır. Bu çalışmadaki amaç, PCOS patogenezinde OS’nin rolünün daha iyi anlaşılmasını sağlamaktır. Çalışmada yaşları 15-22 arasında değişen 30 PCOS’lu hasta ve 30 sağlıklı adolesan ve genç erişkin kadınlar yer almıştır. PCOS tanısı son Amsterdam ESHRE/ASRM konferansı önerileri dikkate alınarak koyuldu. Erken folliküler fazdaki bazal hormon düzeyleri, açlık glukoz ve insülin değerleri, serum lipid profilleri, serum albümin, iskemi modifiye albümin (İMA), total tiyol, native tiyol, disülfid düzeyleri hastaların ana parametreleri olarak kaydedildi. PCOS ve kontrol grubu arasında albümin, İMA, tiyol, disülfid düzeyleri karşılaştırılmıştır. Albümin seviyesi PCOS grubunda kontrol grubuna göre istatistiksel olarak anlamlı yüksek bulunmuştur. Serum CRP düzeyleri ile serum albümin, native tiyol, total tiyol düzeyleri arasında negatif korelasyon saptanmıştır. Total ve native tiyol değerleri VKİ aşırı kilolu olan grupta istatistiksel olarak anlamlı derecede düşük çıkmıştır. Sonuç olarak 30 PCOS, 30 sağlıklı adolesan ve genç erişkin dönemdeki kadınlardaki OS belirteçlerinin incelendiği çalışmada, serum albümin düzeyi PCOS grubunda kontrol grubuna göre istatistiksel olarak anlamlı yüksek bulundu. İMA, total ve native tiyol, disülfid düzeyleri gruplar arasında farklılık tespit edilmedi. OS’in PCOS tanısındaki yerinin net olarak anlaşılması için daha geniş hasta gruplarının ve reprodüktif dönemdeki erişkin kadınların dahil edildiği çalışmalarla desteklenmesi gerekmektedir.
References
- Fauser, B.C, Tarlatzis, B.C, Rebar, R.W. et al. (2012). “Consensus on Women's Health Aspects of Polycystic Ovary Syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group, 97(1), 28-38
- Hart, R. and Doherty, D.A. (2015). “The Potential Implications of A PCOS Diagnosis on A Woman's Long-Term Health Using Data Linkage. The Journal of Clinical Endocrinology and Metabolism, 100 (3), 911-9.
- Peña, A.S, Witchel, S.F, Hoeger, K.M. et al. (2020). “Adolescent Polycystic Ovary Syndrome According To The International Evidence-Based Guideline”. BMC Medicine, 18 (1), 72.
- Ajmal, N, Khan, S.Z. and Shaikh, R. (2019). “Polycystic Ovary Syndrome (PCOS) and Genetic Predisposition: A Review Article”. European Journal Of Obstetrics & Gynecology and Reproductive Biology, X, 3, 100060.
- Barber, T.M, Dimitriadis, G.K, Andreou, A, et al. (2015). “Polycystic Ovary Syndrome: Insight Into Pathogenesis and A Common Association With Insulin Resistance”. Clin Med (Lond), 15 (6), 72-76.
- Repaci, A, Gambineri, A. and Pasquali, R. (2011). “The Role of Low-Grade Inflammation in The Polycystic Ovary Syndrome”. Mol Cell Endocrinol. 335 (1), 30-41.
- Visser, J.A. (2021). “The Importance of Metabolic Dysfunction in Polycystic Ovary Syndrome”. Nature reviews Endocrinology, 17 (2), 77-78.
- McCord, J.M. (1993). “Human Disease, Free Radicals, and The Oxidant/Antioxidant Balance.” Clin Biochem, 26 (5), 351-357.
- Gergerlioglu, H.S, Savas, H.A, Bulbul, F. et al. (2007). “Changes in Nitric Oxide Level and Superoxide Dismutase Activity during Antimanic Treatment”. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 31 (3), 697-702.
- Macut, D, Bjekic-Macut, J. and Savic-Radojevic, A. (2013). “Dyslipidemia and Oxidative Stress in PCOS”. Front Horm Res, 40, 51-63.
- Bar-Or, D, Rael, L.T, Bar-Or, R. et al. (2008). “The Cobalt-Albumin Binding Assay: Insights into Its Mode of Action”. Clin Chim Acta, 387 (1-2), 120-127.
- Ustun, Y, Engin-Ustun, Y, Ozturk, O. et al. (2011). “Ischemia-Modified Albumin As An Oxidative Stress Marker In Preeclampsia”. The journal of Maternal-Fetal & Neonatal Medicine: The Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstet, 24 (3), 418-21.
- Ukinc, K, Eminagaoglu, S, Ersoz, H.O. et al. (2009). “A Novel Indicator of Widespread Endothelial Damage and Ischemia in Diabetic Patients: Ischemia-Modified Albumin”. Endocrine, 36 (3), 425-32.
- Seshadri, Reddy, V, Bukke, S. and Munikumar, M. (2018). “Elevated Levels of The Circulatory Ischemia-Modified Albumin in Patients with Polycystic Ovary Syndrome: A Meta-Analysis.” Gynecol Endocrinol. 34 (10), 868-874.
- Erel, O. and Neselioglu, S. (2014). “A Novel and Automated Assay for Thiol/Disulphide Homeostasis”. Clin Biochem. 47 (18), 326-32.
- Dean, R.T, Fu, S, Stocker, R. et al. (1997). “Biochemistry and Pathology of Radical-Mediated Protein Oxidation.” The Biochemical Journal, 324 (1), 1-18.
- Murri, M, Luque-Ramirez, M, Insenser, M. et al. (2013). “Circulating Markers of Oxidative Stress and Polycystic Ovary Syndrome (PCOS): A Systematic Review and Meta-Analysis”. Hum Reprod Update, 19 (3), 268-88.
- Kanafchian, M, Esmaeilzadeh, S, Mahjoub, S, et al. (2020). “Status of Serum Copper, Magnesium, and Total Antioxidant Capacity in Patients with Polycystic Ovary Syndrome.” Biological Trace Element Research, 193 (1), 111-117.
- Turan, V, Sezer, E.D, Zeybek, B, et al. (2015). “Infertility and The Presence of Insulin Resistance Are Associated with Increased Oxidative Stress in Young, Non-Obese Turkish Women with Polycystic Ovary Syndrome”. Journal of Pediatric and Adolescent Gynecology, 28 (2), 119-23.
- Nasiri, N, Moini, A, Eftekhari-Yazdi, P. et al. (2015). “Abdominal Obesity Can Induce both Systemic and Follicular Fluid Oxidative Stress Independent from Polycystic Ovary Syndrome”. Eur J Obstet Gynecol Reprod Biol, 184, 112-126.
- Suresh, S. and Vijayakumar, T. (2015). “Correlations of Insulin Resistance and Serum Testosterone Levels with LH:FSH Ratio and Oxidative Stress in Women with Functional Ovarian Hyperandrogenism.” Indian Journal of Clinical Biochemistry : IJCBİ, 30 (3), 345-50.
- Ozler, S, Oztas, E. and Tokmak, A. et al. (2016). “The Association of thiol/disulphide Homeostasis and Lipid Accumulation Index with Cardiovascular Risk Factors in Overweight Adolescents with Polycystic Ovary Syndrome”. Clin Endocrinol (Oxf), 84 (4), 516-23.
- Baskol, G, Aygen, E, Erdem, F. et al. (2012). “Assessment of Paraoxonase 1, Xanthine Oxidase and Glutathione Peroxidase Activities, Nitric Oxide and Thiol Levels in Women with Polycystic Ovary Syndrome”. Acta Obstet Gynecol Scand, 91 (3), 326-30.
- Beyazit, F, Yilmaz, N, Balci, O. et al. (2016). “Evaluation of Oxidative Stress in Women with Polycystic Ovarian Syndrome as Represented by Serum Ischemia Modified Albumin and Its Correlation with Testosterone and Insulin Resistance”. Internal Medicine (Tokyo, Japan), 55 (17), 2359-64.
- Samy, N, Hashim, M, Sayed, M, et al. (2009). “Clinical Significance of Inflammatory Markers in Polycystic Ovary Syndrome: Their Relationship to Insulin Resistance and Body Mass Index”. Disease Markers, 26 (4), 163-70.
- Caglar, G.S, Oztas, E, Karadag, D. et al. (2011). “Ischemia-Modified Albumin and Cardiovascular Risk Markers in Polycystic Ovary Syndrome with or Without Insulin Resistance.” Fertility and Sterility, 95 (1), 310-313.
- Cakir, E, Ozbek, M, Ozkaya, E. et al. (2011). “Oxidative Stress Markers are Not Valuable Markers in Lean and Early Age of Polycystic Ovary Syndrome Patients.” J Endocrinol Invest, 34 (7), e178-82.
- Franks, S, Gharani, N, Waterworth, D. et al. (1997). “The Genetic Basis of Polycystic Ovary Syndrome.” Hum Reprod, 12 (12), 2641-2648.
- Shang, Y, Zhou, H, Hu, M, et al. (2020). “Effect of Diet on Insulin Resistance in Polycystic Ovary Syndrome”. The Journal of Clinical Endocrinology and Metabolism, 105 (10), 425.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Articles |
| Authors | |
| Publication Date | December 29, 2021 |
| Published in Issue | Year 2021 Volume: 10 Issue: 4 |
