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Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi

Year 2018, Volume: 1 Issue: 1, 5 - 8, 22.12.2018

Tuğrul Ertuğrul Şerife Tütüncü

Abstract

Kulak zarının temel fonksiyonu ses titreşimlerini dış ortamdan alarak kulak kemikçiklerine iletmektir. Yara iyileşmesinde mast hücrelerinin sayılarının arttığı bildirilmektedir. Bu çalışmada, deneysel pars tensa perforasyonunda mast hücrelerinin sayı ve heterojenitesi incelenmiştir. Pars tensa %10 formol’de tespit edilip yıkandıktan sonra rutin histolojik doku takibi prosedürlerinden geçirilerek parafinde bloklandı. Alınan kesitler toluidin blue ve alcian blue/safranin O kombine boya metodu ile boyandı. Tüm gruplarda, pars tensada mast hücreleri metakromatik, alcian blue (+) ve safranin O (+) boyandı. İyileşme sürecinde en fazla sayıda mast hücresine nar ekstratı verilmiş grupta rastlandı. 

Keywords

Pars tensa perforasyon nar ekstratı mast hücresi

References

  • Araújo MM, Murashima AA, Alves VM, Jamur MC, Hyppolito MA. 2014. Spontaneous healing of the tympanic membrane after traumatic perforation in rats. Brazilian Journal of Otorhinolaryngology, 80(4): 330-8.
  • Shen Y, Guo Y, Du C, Wilczynska M, Hellström S, Ny T. 2012 Mice deficient in urokinase-type plasminogen activator have delayed healing of tympanic membrane perforations. PLoS One, 7(12): 51303.
  • Santa Maria PL, Atlas MD, Ghassemifar R. 2007. Chronic tympanic membrane perforation: a better animal model is needed. Wound Repair and Regeneration, 15(4): 450-8.
  • Stenfeldt K, Johansson C, Hellström S. 2006. The collagen structure of the tympanic membrane: collagen types I, II, and III in the healthy tympanic membrane, during healing of a perforation, and during infection. Otolaryngology – Head & Neck Surgery, 132(3) :293-8.
  • Santa Maria PL, Redmond SL, Atlas MD, Ghassemifar R. 2010. Histology of the healing tympanic membrane following perforation in rats. Laryngoscope, 120(10): 206170.
  • Stenfors LE, Bloom GD, Hellström S. 1984. The tympanic membrane. Acta oto-laryngologica, 414: 28-30.
  • Krystel-Whittemore M, Dileepan KN, Wood JG. 2016. Mast cell: a multi- functional master cell. Frontiers in Immunology, 6, 620.
  • Wernersson S, Pejler G. 2014. Mast cell secretory granules: armed for battle. Nature Reviews Immunology, 14(7): 478-94.
  • Lın T, Befus AD. 2002. Mast cells in mucosal defensis and pathogenesis. ın: mucosal ımmunity volume 1, Ed.: J. Mestecky, M. E. Lamm, W. Strober, J. Bienestock, J. R. McGhee, L. Mayer. Elsevier Acedemic Press, USA. 703710p.
  • Enerback L. 1966. Mast cells in rat gastrointestinal mucosa: 1. effects of fixation. Acta pathologica et microbiologica Scandinavica, 66: 289-302.
  • Cayé-Thomasen P, Tos M. 1996. Mast cell clusters in pars tensa membranae tympani in acute otitis media: a possible role in perforation healing. Acta Oto-Laryngologica, 116(6): 845-9.
  • Wulff BC, Parent AE, Meleski MA, DiPietro LA, Schrementi ME, Wilgus TA. 2012. Mast cells contribute to scar formation during fetal wound healing. Journal of Investigative Dermatology, 132(2): 458-65.
  • Crossmon O. 1937. A modifi cation of mallory’s connective tissue stain with a discussion of the principles ınvolued. The Anatomical Record, 69: 31-38.
  • Enerback L. 1966. Mast Cells in rat gastrointestinal mucosa: 2. dye-binding and metachromatic properties. Acta pathologica et microbiologica Scandinavica, 66, 303-312.
  • Böck P. 1989. Romeis Mikropische Technik. Urban und Schwarzenberg, Aufl, München, Wien, Baltimore.
  • Freld A. 2009. Discovering statistics using SPSS, 3rd edition, Sage, London.
  • Ertuğrul T, Çiftci G, Tütüncü Ş. 2017. Formaldehite maruz kalmış ratlarda böbrek dokusunda mast hücrelerinin dağılımı ve heterojenitesi. Veteriner Hekimler Derneği Dergisi, 88(2): 26-37.
  • Özen A, Bayraktaroğlu AG, Ertuğrul T, Özcan Z, Ceylan A, Özen D. 2014. Domuz oviduktunda mast hücreleri üzerinde ışık ve elektron mikroskobik çalışmalar. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 61, 9–14.
  • Mattsson C, Johansson C, Hellström S. 1999. Myringosclerosis develops within 9h of myringotomy. Otorhinolaryngol Relat Spec, 61 (1): 31-6.
  • Eriksson PO, Mattsson C, Hellstrom S. 2003. First forty-eight hours of developing otitis media: An experimental study. Annals of Otology, Rhinology & Laryngology, 112, 558-66.
  • Spratley JE, Hellström SO, Mattsson CK, Pais-Clemente M. 2001. Topical ascorbic acid reduces myringosclerosis in perforated tympanic membranes. A study in the rat. Annals of Otology, Rhinology & Laryngology, 110 (6): 585-91.
  • Spratley J, Hellström S, Eriksson PO, Pais-Clemente M. 2002. Myringotomy delays the tympanic membrane recovery in acute otitis media: a study in the rat model. Laryngoscope, 112: 1474-81.
  • Santa Maria PL, Redmond SL, Atlas M.D, Ghassemifar R. 2010. Histology of the healing tympanic membrane following perforation in rats. Laryngoscope, 120 (10): 2061-70.
  • Schmidt SH, Hellström S, Carlsöö B. 1984. Shortterm effect of local anaesthetic agents on the structure of the rat tympanic membrane. Arch Otorhinolaryngology, 240: 159-166.
  • Spandow O, Hellström S, Schmid SH. 1990. Hydrocortisone delay of tissue repair of experimental tympanic membrane perforations. Annals of Otology, Rhinology & Laryngology, 99 (8): 647-53.
  • Cayé-Thomasen P, Tos M. 1996. Mast cell clusters in pars tensa membranae tympani in acute otitis media: a possible role in perforation healing. Acta Oto-Laryngologica, 116(6): 845-9.
  • Watanabe T, Kawauchi H, Fujiyoshi T, Mogi G. 1991. Distribution of mast cells in the tubotympanum of guinea pigs. Annals of Otology, Rhinology & Laryngology, 100: 407-12.
  • Wulff BC, Parent AE, Meleski MA, DiPietro LA, Schrementi ME, Wilgus TA. 2012. Mast cells contribute to scar formation during fetal wound healing. Journal of Investigative Dermatology, 132(2) :458-65.
  • Eren U, Kum S, Sandıkcı M, Kara E. 2006. Effects of long-term passive smoking on the mast cells in rat lungs. Revue de Médecine Vétérinaire, 157, 6, 319-322.

Year 2018, Volume: 1 Issue: 1, 5 - 8, 22.12.2018

Tuğrul Ertuğrul Şerife Tütüncü

Abstract

References

  • Araújo MM, Murashima AA, Alves VM, Jamur MC, Hyppolito MA. 2014. Spontaneous healing of the tympanic membrane after traumatic perforation in rats. Brazilian Journal of Otorhinolaryngology, 80(4): 330-8.
  • Shen Y, Guo Y, Du C, Wilczynska M, Hellström S, Ny T. 2012 Mice deficient in urokinase-type plasminogen activator have delayed healing of tympanic membrane perforations. PLoS One, 7(12): 51303.
  • Santa Maria PL, Atlas MD, Ghassemifar R. 2007. Chronic tympanic membrane perforation: a better animal model is needed. Wound Repair and Regeneration, 15(4): 450-8.
  • Stenfeldt K, Johansson C, Hellström S. 2006. The collagen structure of the tympanic membrane: collagen types I, II, and III in the healthy tympanic membrane, during healing of a perforation, and during infection. Otolaryngology – Head & Neck Surgery, 132(3) :293-8.
  • Santa Maria PL, Redmond SL, Atlas MD, Ghassemifar R. 2010. Histology of the healing tympanic membrane following perforation in rats. Laryngoscope, 120(10): 206170.
  • Stenfors LE, Bloom GD, Hellström S. 1984. The tympanic membrane. Acta oto-laryngologica, 414: 28-30.
  • Krystel-Whittemore M, Dileepan KN, Wood JG. 2016. Mast cell: a multi- functional master cell. Frontiers in Immunology, 6, 620.
  • Wernersson S, Pejler G. 2014. Mast cell secretory granules: armed for battle. Nature Reviews Immunology, 14(7): 478-94.
  • Lın T, Befus AD. 2002. Mast cells in mucosal defensis and pathogenesis. ın: mucosal ımmunity volume 1, Ed.: J. Mestecky, M. E. Lamm, W. Strober, J. Bienestock, J. R. McGhee, L. Mayer. Elsevier Acedemic Press, USA. 703710p.
  • Enerback L. 1966. Mast cells in rat gastrointestinal mucosa: 1. effects of fixation. Acta pathologica et microbiologica Scandinavica, 66: 289-302.
  • Cayé-Thomasen P, Tos M. 1996. Mast cell clusters in pars tensa membranae tympani in acute otitis media: a possible role in perforation healing. Acta Oto-Laryngologica, 116(6): 845-9.
  • Wulff BC, Parent AE, Meleski MA, DiPietro LA, Schrementi ME, Wilgus TA. 2012. Mast cells contribute to scar formation during fetal wound healing. Journal of Investigative Dermatology, 132(2): 458-65.
  • Crossmon O. 1937. A modifi cation of mallory’s connective tissue stain with a discussion of the principles ınvolued. The Anatomical Record, 69: 31-38.
  • Enerback L. 1966. Mast Cells in rat gastrointestinal mucosa: 2. dye-binding and metachromatic properties. Acta pathologica et microbiologica Scandinavica, 66, 303-312.
  • Böck P. 1989. Romeis Mikropische Technik. Urban und Schwarzenberg, Aufl, München, Wien, Baltimore.
  • Freld A. 2009. Discovering statistics using SPSS, 3rd edition, Sage, London.
  • Ertuğrul T, Çiftci G, Tütüncü Ş. 2017. Formaldehite maruz kalmış ratlarda böbrek dokusunda mast hücrelerinin dağılımı ve heterojenitesi. Veteriner Hekimler Derneği Dergisi, 88(2): 26-37.
  • Özen A, Bayraktaroğlu AG, Ertuğrul T, Özcan Z, Ceylan A, Özen D. 2014. Domuz oviduktunda mast hücreleri üzerinde ışık ve elektron mikroskobik çalışmalar. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 61, 9–14.
  • Mattsson C, Johansson C, Hellström S. 1999. Myringosclerosis develops within 9h of myringotomy. Otorhinolaryngol Relat Spec, 61 (1): 31-6.
  • Eriksson PO, Mattsson C, Hellstrom S. 2003. First forty-eight hours of developing otitis media: An experimental study. Annals of Otology, Rhinology & Laryngology, 112, 558-66.
  • Spratley JE, Hellström SO, Mattsson CK, Pais-Clemente M. 2001. Topical ascorbic acid reduces myringosclerosis in perforated tympanic membranes. A study in the rat. Annals of Otology, Rhinology & Laryngology, 110 (6): 585-91.
  • Spratley J, Hellström S, Eriksson PO, Pais-Clemente M. 2002. Myringotomy delays the tympanic membrane recovery in acute otitis media: a study in the rat model. Laryngoscope, 112: 1474-81.
  • Santa Maria PL, Redmond SL, Atlas M.D, Ghassemifar R. 2010. Histology of the healing tympanic membrane following perforation in rats. Laryngoscope, 120 (10): 2061-70.
  • Schmidt SH, Hellström S, Carlsöö B. 1984. Shortterm effect of local anaesthetic agents on the structure of the rat tympanic membrane. Arch Otorhinolaryngology, 240: 159-166.
  • Spandow O, Hellström S, Schmid SH. 1990. Hydrocortisone delay of tissue repair of experimental tympanic membrane perforations. Annals of Otology, Rhinology & Laryngology, 99 (8): 647-53.
  • Cayé-Thomasen P, Tos M. 1996. Mast cell clusters in pars tensa membranae tympani in acute otitis media: a possible role in perforation healing. Acta Oto-Laryngologica, 116(6): 845-9.
  • Watanabe T, Kawauchi H, Fujiyoshi T, Mogi G. 1991. Distribution of mast cells in the tubotympanum of guinea pigs. Annals of Otology, Rhinology & Laryngology, 100: 407-12.
  • Wulff BC, Parent AE, Meleski MA, DiPietro LA, Schrementi ME, Wilgus TA. 2012. Mast cells contribute to scar formation during fetal wound healing. Journal of Investigative Dermatology, 132(2) :458-65.
  • Eren U, Kum S, Sandıkcı M, Kara E. 2006. Effects of long-term passive smoking on the mast cells in rat lungs. Revue de Médecine Vétérinaire, 157, 6, 319-322.
There are 29 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Articles
Authors

Tuğrul Ertuğrul

Şerife Tütüncü This is me

Publication Date December 22, 2018
Published in Issue Year 2018 Volume: 1 Issue: 1

Cite

APA Ertuğrul, T., & Tütüncü, Ş. (2018). Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi. International Journal of Veterinary and Animal Research, 1(1), 5-8.
AMA Ertuğrul T, Tütüncü Ş. Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi. IJVAR. December 2018;1(1):5-8.
Chicago Ertuğrul, Tuğrul, and Şerife Tütüncü. “Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi”. International Journal of Veterinary and Animal Research 1, no. 1 (December 2018): 5-8.
EndNote Ertuğrul T, Tütüncü Ş (December 1, 2018) Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi. International Journal of Veterinary and Animal Research 1 1 5–8.
IEEE T. Ertuğrul and Ş. Tütüncü, “Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi”, IJVAR, vol. 1, no. 1, pp. 5–8, 2018.
ISNAD Ertuğrul, Tuğrul - Tütüncü, Şerife. “Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi”. International Journal of Veterinary and Animal Research 1/1 (December 2018), 5-8.
JAMA Ertuğrul T, Tütüncü Ş. Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi. IJVAR. 2018;1:5–8.
MLA Ertuğrul, Tuğrul and Şerife Tütüncü. “Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi”. International Journal of Veterinary and Animal Research, vol. 1, no. 1, 2018, pp. 5-8.
Vancouver Ertuğrul T, Tütüncü Ş. Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi. IJVAR. 2018;1(1):5-8.