Isolation and morphotyping of Acanthamoeba spp. and Vermamoeba spp. from hospital air-conditioning systems
Abstract
Aim: The aim of the study was to investigate the presence of free-living amoebae (FLA) such as Acanthamoeba and Vermamoeba in air conditioning systems of Cumhuriyet University Research and Application Hospital (CURAH).
Methods: For this purpose, twenty-four dust samples were collected using sterile cotton swaps from grilles and filters of five different unit air-conditioners of CURAH located in Sivas, Turkey. Samples were transferred in a sterile tubes to the laboratory and spread onto non-nutrient agar (NNA) plates with a lawn of inactive Escherichia coli. Culture plates were incubated at 30oC. FLA genera was identified according to morphotyping keys.
Results: FLA were found in 14 (58.3%) of examined 24 air-conditioner. Ten (41.6%)were identified as Vermamoeba spp, 4 (16.7%) as Acanthamoeba spp., after microscobic examination.
Conclusion: These findings should alert us on the risk of Acanthamoeba and Vermamoeba infections. In addition, these amoebas could be host for bacteria, fungi and viruses and indirectly become agents of transmission for these pathogens. For decrease the risk both Acanthamoeba and their endosymbionts, regularly cleaning of air-conditioner filters and grilles using effective disinfectants are essential.
Keywords
Details
Primary Language
English
Subjects
Health Care Administration
Journal Section
-
Publication Date
March 28, 2017
Submission Date
June 27, 2016
Acceptance Date
-
Published in Issue
Year 2017 Volume: 39 Number: 1
Cited By
Amoebicidal activity of benzothiazole on Acanthamoeba castellanii cysts and trophozoites and its cytotoxic potentials
Acta Tropica
https://doi.org/10.1016/j.actatropica.2019.105322Genotyping by Sequencing of Acanthamoeba and Naegleria Isolates from the Thermal Pool Distributed Throughout Turkey
Acta Parasitologica
https://doi.org/10.2478/s11686-019-00148-3Isolation and Identification of Pathogenic <i>Acanthamoeba</i> Species from Air Conditioning Systems, Egypt
Japanese Journal of Infectious Diseases
https://doi.org/10.7883/yoken.JJID.2020.049The amoebicidal activity of three substances derived from benzothiazole on Acanthamoeba castellanii cysts and trophozoites and its cytotoxic potentials
Acta Tropica
https://doi.org/10.1016/j.actatropica.2021.105981Molecular Identification, Isolation, Morphologic and Serologic of Acanthamoeba sp. Throw the Ovary of Sprague-Dawley Rats
Pakistan Journal of Biological Sciences
https://doi.org/10.3923/pjbs.2023.124.130The amoebicidal effect of New Bioactive o-Phenylenediamine Surface Functionalized Poly (Maleic Anhydride-alt-Vinyl Acetate) and Citric Acid Derived Carbon Quantum Nanodot-Based Conjugates on Acanthamoeba castellanii cysts and trophozoites and its cytotoxic potentials
Middle Black Sea Journal of Health Science
https://doi.org/10.19127/mbsjohs.1787320