Year 2012,
Volume: 34 Issue: 4, 386 - 392, 28.08.2012
Abstract
References
- Kumar V, Cotran R, Robbins S. Temel Patoloji (Çeviri). Çev. ed. Çevikbaş U. İstanbul, Nobel Tıp Kitabevi, 2000; pp: 133-74.
- Başaran N. Tıbbı Genetik (6. Baskı). Bilim ve Teknik Yayınevi, İstanbul, 1996.
- Çolak A, Pınarbaşı E. Tıbbı Biyoloji. Cumhuriyet Üniversitesi Yayınları. Sivas, 1994; 57: 185-8.
- Kayaalp O. Tıbbi Farmakaloji, Güneş Kitabevi, Ankara, 1994; I: 303-85.
- Yenerman M. ed. Genel Patoloji. Nobel Tıp Kitabevi, İstanbul, 1994; 1362-77.
- Brown LM, Malkinson AM, Rannels DE, Rannels SR. Compensatory lung growth after partial pneumonectomy enhances lung tumorigenesis induced by 3- methylcholanthrene. Canter Res 1999; 59: 5089-92.
- Yenerman M. ed. Genel Patoloji. Nobel Tıp Kitabevi, İstanbul 1994; 1362-77.
- Sezgin İ. Klinik Genetik, Cumhuriyet Üniversitesi Yayınları, Sivas 1998; 70:
- Shimokado K, Watanabe H, Sumii M, Miyagawa K, Kamiya K, Dohi K, Niwa O. p53 gene mutation and loss of heterozygosity of chromosome 11 in methylcholanthrene-induced mouse sarcomas. Jpn J Cancer Res 1998; 89: 269- 77.
- Macdonald F, Ford CHJ. Oncogenes and Tumor Supressor Genes. BIOS Scientific Publishers Limited, Printed by İnformation Pres Ltd, Oxford, U.K., 1991.
- Ruddon RW. Cancer Biology. Oxford University Press. 236, Newyork, Oxford 1995.
- Vähäkangas KH, Bennett WP, Castrén K, Welsh JA, Khan MA, Blömeke B, Alavanja MC, Harris CC. p53 and K-ras mutations in lung cancers from former and never-smoking women. Cancer Res 2001; 61: 4350-6.
- DeMarini DM, Landi S, Tian D, Hanley NM, Li X, Hu F, Roop BC, Mass MJ, Keohavong P, Gao W, Olivier M, Hainaut P, Mumford JL Lung tumor KRAS and TP53 mutations in nonsmokers reflect exposure to PAH-rich coal combustion emissions. Cancer Res 2001; 61: 6679-81.
- Malory M, Nelkin B, Baylin S. Euolutionary Model of lung Cancer Lung Cancer: Principles and Practice, Philadelphia, 1996.
- Rollins LA, Leone-Kabler S, O'Sullivan MG, Miller MS. Role of tumor suppressor genes in transplacental lung carcinogenesis. Mol Carcinog 1998; 21: 177-84.
- Horio Y, Chen A, Rice P, Roth JA, Malkinson AM, Schrump DS. Ki-ras and p53 mutations are early and late events, respectively, in urethane-induced pulmonary carcinogenesis in A/J mice. Mol Carcinog 1996; 17: 217-23.
- Yuspa SH. Overview of carcinogenesis: past, present and future. Carcinogenesis 2000; 21: 341-4.
- Li ZH, Zheng J, Weiss LM, Shibata D. c-k-ras and p53 mutations occur very early in adenocarcinoma of the lung. Am J of Pethol 1994; 144: 303-9.
- Malkinson AM, Koski KM, Evans WA, Festing MF. Butylated hydroxytoluene exposure is necessary to induce lung tumors in BALB mice treated with 3- methylcholanthrene. Cancer Res 1997; 57: 2832-4.
- Balmain A, Harris CC. Carcinogenesis in mouse and human cells: parallels and paradoxes. Carcinogenesis 2000; 21: 371-7.
- Rihn BH, Bottin MC, Coulais C, Rouget R, Monhoven N, Baranowski W, Edorh A, Keith G. Genotoxicity of 3-methylcholanthrene in liver of transgenic big Blue mice. Environ Mol Mutagen 2000; 36: 266-73.
- Niwa O, Kamiya K, Furihata C, Nitta Y, Wang Z, Fan YJ, Ninomiya Y, Kotomura N, Numoto M, Kominami R. Association of minisatellite instability with c-myc amplification and K-ras mutation in methylcholanthrene-induced mouse sarcomas. Cancer Res 1995; 55: 5670-6.
- Gressani KM, Leone-Kabler S, O'Sullivan MG, Case LD, Malkinson AM, Miller MS. Strain-dependent lung tumor formation in mice transplacentally exposed to 3-methylcholanthrene and post-natally exposed to butylated hydroxytoluene. Carcinogenesis 1999; 20: 2159-65.
- Lee C, Riddick DS. Transcriptional suppression of cytochrome P450 2C11 gene expression by 3-methylcholanthrene. Biochem Pharmacol 2000; 59: 1417-23.
- Watanabe H, Shimokado K, Asahara T, Dohi K, Niwa O. Analysis of the c-myc, K-ras and p53 genes in methylcholanthrene-induced mouse sarcomas. Jpn J Cancer Res 1999; 90: 40-7.
- Williams GM, Iatropoulos MJ, Whysner J. Safety assessment of butylated hydroxyanisole and butylated hydroxytoluene as antioxidant food additives. Food Chem Toxicol 1999; 37: 1027-38.
- Witschi HP, Morse CC. Cell kinetics in mouse lung following administration of carcinogens and butylated hydroxytoluene. Toxicol Appl Pharmacol 1985; 78:
- Malkinson AM, Belinsky SA. Lung Canner, Lippincott-Raven Publishers, Philadelphia 1996; 273-83.
3-Metilkolantren ve bütil hidroksitoluenle uyarılmış rat akciğer dokularında p-53 ekson 8 geni mutasyon analizi
Abstract
Amaç. Bu çalışmada, gıda katkı maddesi olarak kullanılan Bütillenmiş hidroksitoluen (BHT)’in sadece kendisinin ve ayrıca BHT’nin bir kimyasal karsinojen olan 3-Metilkolantren (3-MC) ile birlikte uyğulandığında rat akciğerlerindeki karsinojen potansiyelleri ve p53 ekzon 8 geni üzerindeki mutasyon etkisinin incelenmesi amaçlanmıştır. Yöntem. Bu çalışmada 52 erkek rat kullanılmıştır. Deneye alınan birinci gruptaki (D1 grubu) 15 rata, 6 kez 3-MC (40 mg/kg/hafa dozda ip yolla) uygulanmıştır. Deneye alınan ikinci gruptaki (D2 grubu) 15 rata tek doz 40 mg/kg 3-MC ve ardından 6 kez BHT (200 mg/kg/hafta dozda ip yolla) uygulanmıştır. Deneye alınan üçüncü gruptaki (D3 grubu) 12 rata, sadece 6 kez BHT (200 mg/kg/hafta ip yolla) uygulanmıştır. Ayrıca 10 rat’tan oluşan bir kontrol grubu kullanılmıştır. Ratlar 26 hafta beklenildikten sonra servikal dislokasyon ile öldürülmüştür. Akciğerler sintigrafik, radyolojik ve morfolojik olarak değerlendirilmiştir. Bütün grupların akciğer dokusundan DNA izolasyonu yapılmış ve bu DNA’lardan, P53 geni ekson 8 amplifkasyonu Polimeraz Zincir Reaksiyonu (PCR) ürünlerinde Single-Strand Conformation Polimorphism (SSCP) analizi yapılarak, nokta mutasyonu taraması yapılmıştır. Bulgular. Bu çalışmaların sonucunda radyolojik, sintigrafik, morfolojik ve genetik düzeyde ratların akciğerinde kanser oluşumu tespit edilememiştir. Fakat ratlara kimyasal maddelerin uygulanım yerinde yumuşak doku kaynaklı sarkom gelişimi tespit edilmiştir. Yapılan mutasyon taramasında P53 geni ekson 8’de bir mutasyon bulunamamıştır. Sonuç. Her ne kadar çalışmamızda ratlarda kanser oluşumu tespit edemesekte, uygun doz ve zaman uyumlu 3-MC ve BHT uygulayarak, özellikle tümör oluşumuna duyarlı fare türleri kullanarak çalışmanın uygun olacağı düşünülmüştür.
References
- Kumar V, Cotran R, Robbins S. Temel Patoloji (Çeviri). Çev. ed. Çevikbaş U. İstanbul, Nobel Tıp Kitabevi, 2000; pp: 133-74.
- Başaran N. Tıbbı Genetik (6. Baskı). Bilim ve Teknik Yayınevi, İstanbul, 1996.
- Çolak A, Pınarbaşı E. Tıbbı Biyoloji. Cumhuriyet Üniversitesi Yayınları. Sivas, 1994; 57: 185-8.
- Kayaalp O. Tıbbi Farmakaloji, Güneş Kitabevi, Ankara, 1994; I: 303-85.
- Yenerman M. ed. Genel Patoloji. Nobel Tıp Kitabevi, İstanbul, 1994; 1362-77.
- Brown LM, Malkinson AM, Rannels DE, Rannels SR. Compensatory lung growth after partial pneumonectomy enhances lung tumorigenesis induced by 3- methylcholanthrene. Canter Res 1999; 59: 5089-92.
- Yenerman M. ed. Genel Patoloji. Nobel Tıp Kitabevi, İstanbul 1994; 1362-77.
- Sezgin İ. Klinik Genetik, Cumhuriyet Üniversitesi Yayınları, Sivas 1998; 70:
- Shimokado K, Watanabe H, Sumii M, Miyagawa K, Kamiya K, Dohi K, Niwa O. p53 gene mutation and loss of heterozygosity of chromosome 11 in methylcholanthrene-induced mouse sarcomas. Jpn J Cancer Res 1998; 89: 269- 77.
- Macdonald F, Ford CHJ. Oncogenes and Tumor Supressor Genes. BIOS Scientific Publishers Limited, Printed by İnformation Pres Ltd, Oxford, U.K., 1991.
- Ruddon RW. Cancer Biology. Oxford University Press. 236, Newyork, Oxford 1995.
- Vähäkangas KH, Bennett WP, Castrén K, Welsh JA, Khan MA, Blömeke B, Alavanja MC, Harris CC. p53 and K-ras mutations in lung cancers from former and never-smoking women. Cancer Res 2001; 61: 4350-6.
- DeMarini DM, Landi S, Tian D, Hanley NM, Li X, Hu F, Roop BC, Mass MJ, Keohavong P, Gao W, Olivier M, Hainaut P, Mumford JL Lung tumor KRAS and TP53 mutations in nonsmokers reflect exposure to PAH-rich coal combustion emissions. Cancer Res 2001; 61: 6679-81.
- Malory M, Nelkin B, Baylin S. Euolutionary Model of lung Cancer Lung Cancer: Principles and Practice, Philadelphia, 1996.
- Rollins LA, Leone-Kabler S, O'Sullivan MG, Miller MS. Role of tumor suppressor genes in transplacental lung carcinogenesis. Mol Carcinog 1998; 21: 177-84.
- Horio Y, Chen A, Rice P, Roth JA, Malkinson AM, Schrump DS. Ki-ras and p53 mutations are early and late events, respectively, in urethane-induced pulmonary carcinogenesis in A/J mice. Mol Carcinog 1996; 17: 217-23.
- Yuspa SH. Overview of carcinogenesis: past, present and future. Carcinogenesis 2000; 21: 341-4.
- Li ZH, Zheng J, Weiss LM, Shibata D. c-k-ras and p53 mutations occur very early in adenocarcinoma of the lung. Am J of Pethol 1994; 144: 303-9.
- Malkinson AM, Koski KM, Evans WA, Festing MF. Butylated hydroxytoluene exposure is necessary to induce lung tumors in BALB mice treated with 3- methylcholanthrene. Cancer Res 1997; 57: 2832-4.
- Balmain A, Harris CC. Carcinogenesis in mouse and human cells: parallels and paradoxes. Carcinogenesis 2000; 21: 371-7.
- Rihn BH, Bottin MC, Coulais C, Rouget R, Monhoven N, Baranowski W, Edorh A, Keith G. Genotoxicity of 3-methylcholanthrene in liver of transgenic big Blue mice. Environ Mol Mutagen 2000; 36: 266-73.
- Niwa O, Kamiya K, Furihata C, Nitta Y, Wang Z, Fan YJ, Ninomiya Y, Kotomura N, Numoto M, Kominami R. Association of minisatellite instability with c-myc amplification and K-ras mutation in methylcholanthrene-induced mouse sarcomas. Cancer Res 1995; 55: 5670-6.
- Gressani KM, Leone-Kabler S, O'Sullivan MG, Case LD, Malkinson AM, Miller MS. Strain-dependent lung tumor formation in mice transplacentally exposed to 3-methylcholanthrene and post-natally exposed to butylated hydroxytoluene. Carcinogenesis 1999; 20: 2159-65.
- Lee C, Riddick DS. Transcriptional suppression of cytochrome P450 2C11 gene expression by 3-methylcholanthrene. Biochem Pharmacol 2000; 59: 1417-23.
- Watanabe H, Shimokado K, Asahara T, Dohi K, Niwa O. Analysis of the c-myc, K-ras and p53 genes in methylcholanthrene-induced mouse sarcomas. Jpn J Cancer Res 1999; 90: 40-7.
- Williams GM, Iatropoulos MJ, Whysner J. Safety assessment of butylated hydroxyanisole and butylated hydroxytoluene as antioxidant food additives. Food Chem Toxicol 1999; 37: 1027-38.
- Witschi HP, Morse CC. Cell kinetics in mouse lung following administration of carcinogens and butylated hydroxytoluene. Toxicol Appl Pharmacol 1985; 78:
- Malkinson AM, Belinsky SA. Lung Canner, Lippincott-Raven Publishers, Philadelphia 1996; 273-83.
There are 28 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Basic Science Research Articles |
| Authors | |
| Publication Date | August 28, 2012 |
| Published in Issue | Year 2012Volume: 34 Issue: 4 |