Effect of captopril on the oxidative damage caused by pentylenetetrazole in the SHSY-5Y human neuroblastoma cell line
Year 2020,
Volume: 42 Issue: 4, 479 - 483, 31.12.2020
Murat Doğan
,
Mustafa Karademir
Abstract
Objective: Epilepsy is a crucial brain disorder that seizures could cause a neuronal loss in the hippocampus. Oxidative stress has an important role in the pathology of this way. The aim of this study was to investigate the neuroprotective effect of captopril, on pentylenetetrazole (PTZ) induced epileptic seizures in SH-SY5Y cell line.
Method: In this XTT cell viability assay, captopril was performed in vitro SH-SY5Y cell culture to evaluate PTZ-induced neurotoxicity. Tissue TOS concentrations at the cell supernatants were quantified with the automated assay method. Hydrogen peroxide was used for the calibration of the assay.
Results: When XTT cell viability results were evaluated, captopril did not affect neuronal viability in SH-SY5Y cell line. Moreover, captopril did not have significant effect on TOS levels (***P>0. 001)
Conclusions: Results showed that, captopril did not have neuroprotective properties in SH-SY5Y cell line after PTZ-induced neurotoxicity.
Supporting Institution
Sivas Cumhuriyet University, School of Medicine, CUTFAM Research Center, Sivas, Turkey
Thanks
The authors would like to thank the Sivas Cumhuriyet University, School of Medicine, CUTFAM Research Center, Sivas, Turkey, for providing the necessary facilities to conduct this study.
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Year 2020,
Volume: 42 Issue: 4, 479 - 483, 31.12.2020
Murat Doğan
,
Mustafa Karademir
References
- REFERENCES
1. Karabulut S, Bayramov R, Korkmaz Bayramov K, Filiz AK, Taskıran AS, Ozdemir E. Effect of the allopregnanolone and allotetrahydrodeoxycortico steron on spike-wave discharges in the EEG of absence epilepsy rat models, General Physiology and Biophysics, 2018; 37: 205-211.
- 2. Erel O, A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem, 2004; 37: 112–119.
- 3. Taskıran AŞ, Ergül M, Güneş H, Ozturk A, Sahın B, Ozdemır E. The Effects of Proton Pump Inhibitors (Pantoprazole) on Pentylenetetrazole-Induced Epileptic Seizures in Rats and Neurotoxicity in the SH-SY5Y Human Neuroblastoma Cell Line. Cellular and Molecular Neurobıology, 2020.
- 4. Lamberty Y, Klitgaard H. Consequences of pentylenetetrazole kindling on spatial memory and emotional responding in the rat. Epilepsy Behav, 2000; 1: 256–261.
- 5. Bolterman RJ, Manriquez MC, Ruiz MCO, Juncos LA, Romero JC. Effects of captopril on the renin angiotensin system, oxidative stress, and endothelin in normal and hypertensive rats. Hypertension, 2005; 46: 943–947.
- 6. Ramos SF, Mendonça BP, Lefa DD et al. Effects of neuropeptide S on seizures and oxidative damage induced by pentylenetetrazole in mice. Pharmacol Biochem Behav, 2012; 103: 197–203.
- 7. Potschka H. Animal models of drug-resistant epilepsy. Epileptic Disorder, 2012; 14: 226–234.
- 8. Kalantaripour T, Esmaeili-Mahani S, Sheibani V et al (2017) Apelin-13 protects rat primary cortical glia-neuron co-culture against pentylenetetrazole-induced toxicity. Biomed Pharmacother, 2017; 87: 661–668.
- 9. Tastemur Y, Gumus E, Ergul M, Ulu M, Akkaya R, Ozturk A, Taskıran AŞ. Positive effects of angiotensin-converting enzyme (ACE) inhibitor, captopril, on pentylenetetrazole-induced epileptic seizures in mice. Trop J Pharm Res, 2020; 19: 637–643.
- 10. Whitlock JR., Learning induces long-term potentiation in the hippocampus. Science, 2006; 313: 1093–1097.
- 11. Curia G, Lucchi C, Vinet J, Gualtieri F, Marinelli C, Torsello A, Costantino L, Biagini G. Pathophysiogenesis of Mesial Temporal Lobe Epilepsy: Is Prevention of Damage Antiepileptogenic? Curr Med Chem, 2014; 21(6): 663-688.