Rheum Ribes L.'nin etanol ekstrelerinin malign melanoma hücreleri üzerine anti-kanser etkinliğinin araştırılması
Abstract
Amaç: Işkın bitkisi (Rheum ribes L.) Çin, Hindistan, İran ve Türkiye’de yabani olarak yetişmekte olup birçok tıbbi amaç için kullanılmaktadır. Bu çalışmanın amacı ışkın bitkisinin kök, gövde ve kabuklarının etanol ekstraktlarının malign melanoma hücrelerine karşı anti-kanser etkinliğini araştırmaktır. Gereç ve Yöntem: Bitkinin oda sıcaklığında kurutulan kök, gövde ve kabuk kısımları mikser ile toz haline getirilip %50 etanol ile 24 saat inkübe edildi. Organik faz rotary evaporatörde uçurulup, su fazı liyofilizatörde ayrıldıktan sonra elde edilen ekstraktlarda; öncelikle total fenol ve total flavanoid içerik düzeyi ile total antioksidan kapasite fotometrik yöntemlerle ölçüldü. Daha sonra kültürize edilen malign melanoma hücreleri (B16F10) bu ekstrelerin farklı konsantrasyonlarında 24 saat inkübe edildi. İnkübasyondan sonra sitotoksisite düzeyi; kolorometrik MTT [3-(4,5-diMetilTiyazol-2-il)-2,5-difenil Tetrazolyum bromür] metodu ile, apoptoz düzeyi ise; Akridin Oranj-Etidyum Bromit (AO-EB) ikili boyama yöntemi ile tespit edildi. Bulgular: Hem sitotoksisite hemde apoptoz sonuçları incelediğinde aynı konsantrasyonlarda en yüksek sitotoksik (%87,57±4,3) ve apoptotik (59,76±3,57) etkinin kök ekstreleri ile sağlandığı görüldü (p<0,001). MTT sonuçlarına göre kök ekstrelerinin IC50 değerinin 61 µg/mL ve 500 µg/mL’de hücre ölümünün %91,92±3,9 olduğu tespit edildi. Sonuç: Rheum ribes L. bitkisinin malign melanoma hücreleri üzerine antikanser etkinliğinin var olduğu ve bitkinin gövde ve kabuklarına kıyasla kök ekstraktının anti-kanser ilaç olma potansiyelinin daha yüksek olduğu kanaatine varılmıştır.
Keywords
Supporting Institution
YOK
Project Number
YOK
References
- 1. Bazzaz F, Khajehkaramadin M, Shokooheizadeh HR. In vitro antibacterial activity of Rheum ribes extract obtained from various plant parts against clinical isolates of Gram-negative pathogens. Iranian Journal of Pharmaceutical Research. 2010:87-91.
- 2. Tosun F, Kizilay ÇA. Anthraquinones and flavonoids from Rheum ribes. J Fac Pharm Ankara. 2003;32:31-5.
- 3. Cullen J. Flora of Turkey and the East Aegean Islands. Edinburgh: Edinburgh University Press; 1966.
- 4. Munzuroğlu Ö, Karataş F, Gür N. Işgın ( Rheum ribes L.) Bitkisindeki A, E ve C Vitaminleri ile Selenyum Düzeylerinin Araştırılması. Turk J Biol. 2000;24:397–404.
- 5. Öztürk M, Aydoğmuş-Öztürk F, Duru ME, Topçu G. Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chem. 2007;103:623-30.
- 6. Emen Tanrikut S, Çeken B, Altaş S, Pirinççioğlu M, Kizil G, Kizil M. DNA cleavage protecting activity and in vitro antioxidant potential of aqueous extract from fresh stems of Rheum ribes. Acta Aliment. 2013;42:461-72.
- 7. Abdulla K, Taha E, Rahim S. Phenolic profile, antioxidant and antibacterial effects of ethanol and aqueous extracts of Rheum ribes L. roots. Der Pharmacia Lett. 2014;6:201-5.
- 8. Alaadin AM, Al-Khateeb EH, Jäger AK. Antibacterial activity of the Iraqi Rheum ribes. Root. Pharm Biol. 2007;45:688-90.
- 9. Naemi F, Asghari G, Yousofi H, Yousefi HA. Chemical composition of essential oil and anti trichomonas activity of leaf, stem, and flower of Rheum ribes L. extracts. Avicenna J Phytomed. 2014;4:191.
- 10. Hudson JB, Lee MK, Sener B, Erdemoglu N. Antiviral activities in extracts of Turkish medicinal plants. Pharm Biol. 2000;38:171-5.
- 11. Sardari S, Shokrgozar MA, Ghavami G. Cheminformatics based selection and cytotoxic effects of herbal extracts. Toxicol In Vitro. 2009;23:1412-21.
- 12. Kasabri V, Afifi FU, Hamdan I. In vitro and in vivo acute antihyperglycemic effects of five selected indigenous plants from Jordan used in traditional medicine. J Ethnopharmacol. 2011;133:888-96.
- 13. Khiveh A, Hashempur MH, Shakiba M, et al. Effects of rhubarb ( Rheum ribes L.) syrup on dysenteric diarrhea in children: a randomized, double-blind, placebo-controlled trial. J Integr Med. 2017;15:365-72.
- 14. Hadjzadeh MR, Jafary G. The effects of Rheum Ribes on serum lipids in hyperlipidemic Rabbit. koomesh. 2004;5:19-28.
- 15. Gholamhoseinian A, Moradi MN, Sharifi-Far F. Screening the methanol extracts of some Iranian plants for acetylcholinesterase inhibitory activity. Res Pharm Sci. 2009;4:105-12.
- 16. Esmaeilbeig M, Kouhpayeh SA, Amirghofran Z. An Investigation of the Growth Inhibitory Capacity of Several Medicinal Plants From Iran on Tumor Cell Lines. Iran J Cancer Prev. 2015;8:e4032.
- 17. Achakzai JK, Anwar Panezai M, Kakar MA, et al. In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR). Biomed Res Int. 2019;2019:3264846.
- 18. Aygun A, Gulbagca F, Nas MS, et al. Biological synthesis of silver nanoparticles using Rheum ribes and evaluation of their anticarcinogenic and antimicrobial potential: A novel approach in phytonanotechnology. J Pharm Biomed Anal. 2019:113012.
- 19. Jerant AF, Johnson JT, Sheridan CD, Caffrey TJ. Early detection and treatment of skin cancer. Am Fam Physician. 2000;62:357-68, 75-6, 81-2.
- 20. Slinkard K, Singleton VL. Total Phenol Analysis: Automation and Comparison with Manual Methods. Am J Enol Vitic. 1977;28:49-55.
- 21. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999;64:555-9.
- 22. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38:1103-11.
- 23. Khan MA, Rahman AA, Islam S, et al. A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae). BMC Res Notes. 2013;6:24.
- 24. Galati G, O'Brien PJ. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic Biol Med. 2004;37:287-303.
- 25. Robaszkiewicz A, Balcerczyk A, Bartosz G. Antioxidative and prooxidative effects of quercetin on A549 cells. Cell biology international. 2007;31:1245-50.
- 26. Kocyigit A, Guler EM, Dikilitas M. Role of Antioxidant Phytochemicals in Prevention, Formation and Treatment of Cancer. 2018.
- 27. Zare P, Shahbazfar AA, Alem M, Varmaghani S. Effects of coadministration of artemisinin and iron on histopathologicalalterations in the AGS gastric adenocarcinoma cell line. Turk J Med Sci. 2017;47:364-7.
- 28. Azam S, Hadi N, Khan NU, Hadi SM. Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties. Toxicol In Vitro. 2004;18:555-61.
- 29. Raza H, John A. Green tea polyphenol epigallocatechin-3-gallate differentially modulates oxidative stress in PC12 cell compartments. Toxicol Appl Pharmacol. 2005;207:212-20.
Investigation of the anti-cancer effect of Rheum Ribes L.'s ethanol extracts on malign melanoma cells
Abstract
Objective: Işkın (Rheum ribes L.), China, India, Iran and Turkey are grow in the wild and are used for many medical purposes. The aim of this study is to investigate the anti-cancer effectiveness of ethanol extracts of the roots, stems and bark of the Rheum ribes L against malignant melanoma cells. Material and Method: Root, stem and bark parts of the plant dried at room temperature were powdered with a mixer and incubated with 50% ethanol for 24 hours. In the extracts obtained after the organic phase is evaporated in a rotary evaporator and the water phase is separated in the lyophilizer; firstly, total phenol and total flavanoid content level and total antioxidant capacity were measured by photometric methods. Cultured malignant melanoma cells (B16F10) were incubated at different concentrations of these extracts for 24 hours. After incubation; cytotoxicity levels was determined with the colorometric MTT method [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], and the apoptosis level by Acridine Orange-Ethidium Bromide (AO-EB) double staining method. Results: When examining the results of both cytotoxicity and apoptosis, it was observed that the highest cytotoxic (87.57 ± 4.3%) and apoptotic (59.76 ± 3.57) effects were achieved with root extracts at the same concentrations (p <0.001). According to MTT results, IC50 value of root extracts was determined to be 61 µg/mL and cell death at 500 µg/mL as 91.92 ± 3.9. Conclusion: It has been concluded that Rheum ribes L. plant has anticancer activity on malignant melanoma cells and the root extract has a higher anti-cancer drug potential compared to the stem and barks of the plant.
Keywords
Project Number
YOK
References
- 1. Bazzaz F, Khajehkaramadin M, Shokooheizadeh HR. In vitro antibacterial activity of Rheum ribes extract obtained from various plant parts against clinical isolates of Gram-negative pathogens. Iranian Journal of Pharmaceutical Research. 2010:87-91.
- 2. Tosun F, Kizilay ÇA. Anthraquinones and flavonoids from Rheum ribes. J Fac Pharm Ankara. 2003;32:31-5.
- 3. Cullen J. Flora of Turkey and the East Aegean Islands. Edinburgh: Edinburgh University Press; 1966.
- 4. Munzuroğlu Ö, Karataş F, Gür N. Işgın ( Rheum ribes L.) Bitkisindeki A, E ve C Vitaminleri ile Selenyum Düzeylerinin Araştırılması. Turk J Biol. 2000;24:397–404.
- 5. Öztürk M, Aydoğmuş-Öztürk F, Duru ME, Topçu G. Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chem. 2007;103:623-30.
- 6. Emen Tanrikut S, Çeken B, Altaş S, Pirinççioğlu M, Kizil G, Kizil M. DNA cleavage protecting activity and in vitro antioxidant potential of aqueous extract from fresh stems of Rheum ribes. Acta Aliment. 2013;42:461-72.
- 7. Abdulla K, Taha E, Rahim S. Phenolic profile, antioxidant and antibacterial effects of ethanol and aqueous extracts of Rheum ribes L. roots. Der Pharmacia Lett. 2014;6:201-5.
- 8. Alaadin AM, Al-Khateeb EH, Jäger AK. Antibacterial activity of the Iraqi Rheum ribes. Root. Pharm Biol. 2007;45:688-90.
- 9. Naemi F, Asghari G, Yousofi H, Yousefi HA. Chemical composition of essential oil and anti trichomonas activity of leaf, stem, and flower of Rheum ribes L. extracts. Avicenna J Phytomed. 2014;4:191.
- 10. Hudson JB, Lee MK, Sener B, Erdemoglu N. Antiviral activities in extracts of Turkish medicinal plants. Pharm Biol. 2000;38:171-5.
- 11. Sardari S, Shokrgozar MA, Ghavami G. Cheminformatics based selection and cytotoxic effects of herbal extracts. Toxicol In Vitro. 2009;23:1412-21.
- 12. Kasabri V, Afifi FU, Hamdan I. In vitro and in vivo acute antihyperglycemic effects of five selected indigenous plants from Jordan used in traditional medicine. J Ethnopharmacol. 2011;133:888-96.
- 13. Khiveh A, Hashempur MH, Shakiba M, et al. Effects of rhubarb ( Rheum ribes L.) syrup on dysenteric diarrhea in children: a randomized, double-blind, placebo-controlled trial. J Integr Med. 2017;15:365-72.
- 14. Hadjzadeh MR, Jafary G. The effects of Rheum Ribes on serum lipids in hyperlipidemic Rabbit. koomesh. 2004;5:19-28.
- 15. Gholamhoseinian A, Moradi MN, Sharifi-Far F. Screening the methanol extracts of some Iranian plants for acetylcholinesterase inhibitory activity. Res Pharm Sci. 2009;4:105-12.
- 16. Esmaeilbeig M, Kouhpayeh SA, Amirghofran Z. An Investigation of the Growth Inhibitory Capacity of Several Medicinal Plants From Iran on Tumor Cell Lines. Iran J Cancer Prev. 2015;8:e4032.
- 17. Achakzai JK, Anwar Panezai M, Kakar MA, et al. In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR). Biomed Res Int. 2019;2019:3264846.
- 18. Aygun A, Gulbagca F, Nas MS, et al. Biological synthesis of silver nanoparticles using Rheum ribes and evaluation of their anticarcinogenic and antimicrobial potential: A novel approach in phytonanotechnology. J Pharm Biomed Anal. 2019:113012.
- 19. Jerant AF, Johnson JT, Sheridan CD, Caffrey TJ. Early detection and treatment of skin cancer. Am Fam Physician. 2000;62:357-68, 75-6, 81-2.
- 20. Slinkard K, Singleton VL. Total Phenol Analysis: Automation and Comparison with Manual Methods. Am J Enol Vitic. 1977;28:49-55.
- 21. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999;64:555-9.
- 22. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38:1103-11.
- 23. Khan MA, Rahman AA, Islam S, et al. A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae). BMC Res Notes. 2013;6:24.
- 24. Galati G, O'Brien PJ. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic Biol Med. 2004;37:287-303.
- 25. Robaszkiewicz A, Balcerczyk A, Bartosz G. Antioxidative and prooxidative effects of quercetin on A549 cells. Cell biology international. 2007;31:1245-50.
- 26. Kocyigit A, Guler EM, Dikilitas M. Role of Antioxidant Phytochemicals in Prevention, Formation and Treatment of Cancer. 2018.
- 27. Zare P, Shahbazfar AA, Alem M, Varmaghani S. Effects of coadministration of artemisinin and iron on histopathologicalalterations in the AGS gastric adenocarcinoma cell line. Turk J Med Sci. 2017;47:364-7.
- 28. Azam S, Hadi N, Khan NU, Hadi SM. Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties. Toxicol In Vitro. 2004;18:555-61.
- 29. Raza H, John A. Green tea polyphenol epigallocatechin-3-gallate differentially modulates oxidative stress in PC12 cell compartments. Toxicol Appl Pharmacol. 2005;207:212-20.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Original Article |
| Authors | |
| Project Number | YOK |
| Publication Date | March 19, 2020 |
| Published in Issue | Year 2020 Volume: 3 Issue: 2 |
Cited By
The Effect of Rheum ribes Extract Origin of Elazig Province on Ventilator-Associated Pneumonia and Antioxidant Capacity
Gazi Üniversitesi Fen Bilimleri Dergisi Part C: Tasarım ve Teknoloji
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