Abstract
Amaç: Bu çalışmanın amacı HIV enfeksiyonu pozitif
saptanan hastalarda HHV-6 DNA sıklığının, HHV-6 DNA’sı pozitif saptanan
olgularda ise HHV-6 varyant türünün (varyant A veya varyant B) belirlenmesiydi.
Gereç
ve Yöntem: Çalışmaya 20 HIV pozitif hasta
ile 57 HIV negatif sağlıklı birey dahil edildi. Hasta ve kontrol grubunda HHV-6
DNA’sı polimeraz zincir reaksiyonu (PZR) ile araştırıldı. HHV-6 DNA pozitif
saptanan olgularda HHV-6 varyant türü restriksiyon parça uzunluğu polimorfizmi (restriction
fragment lenght polymorphisim: RFLP) ile
araştırıldı. İstatistiksel değerlendirme SPSS programında yapıldı, p≤0.05
istatistiksel olarak anlamlı kabul edildi.
Bulgular: HIV pozitif hastalarda HHV-6 DNA pozitifliği %45
(9/20), HIV negatiflerde ise %33,3 (19/57) olarak belirlendi. Toplamda
pozitiflik oranı %36,3 (28/77) idi. HIV pozitif hastalarda HHV-6 varyant B
saptanırken, varyant A saptanmadı.
Sonuç: HIV pozitif hastalarda HHV-6 seropozitifliği ve
varyant tiplerinin öneminin belirlenmesi için daha fazla sayıda olgu içeren başka
klinik çalışmalara gereksinim olduğu görüşündeyiz.
Keywords
References
- 1. Braun DK, Dominguez G, Pellet PE. Human Herpesvirus-6. Clin Mic Rev 1997; 10: 521-67.
- 2. Dolcetti R, Luca DD, Mİrandola P, et al. Frequent detection of Human Herpesvirus- 6 DNA in HIV associated lymphoadenopathy. Lancet 1994; 344: 543.
- 3. Fox J, Briggs M, Tedder RS. Antibody to human herpesvirus 6 in HIV-1 positive and negative homosexual men. Lancet1988; ii, 396-7.
- 4. Spira TJ, Bozeman LH, Sanderlin KC, et al. Lack of correlation between human herpesvirus-6 infection and the course of human immunodeficiency virus infection. J Infect Dis 1989; 161: 567-70.
- 5. Corbellino M, Lusso P, Gallo RC. Disseminated human herpesvirus 6 infection in AIDS. Lancet 1993; 342: 1242.
- 6. Lindquester GJ, Inoue N, Allen RD, et al. Restriction endonuclease mapping and molecular cloning of the Human Herpesvirus 6 variant B strain Z29 genome. Arch Virol1996; 141: 367-79.
- 7. Aubin JT, Collandre H, Candotti D, et al. Several groups among human herpesvirus 6 strains can be distinguished by southernblotting and polymerase chain reaction. J Clin Microbiol 1991; 29, 367-72.
- 8. Kositanont U, Wasi C, Wanprapar N, et al. Primary infection of human herpesvirus 6 in children with infection of human immunodeficiency virus type 1. J Infect Dis 1990; 180: 50-5.
- 9. Munawwar A, Singh S. Human herpesviruses as copathogens of HIV infection, Their role in HIV transmission, and disease progression. J Lab Physicians 2016; 8: 5-18.
- 10. Contreras A, Mardirossian A, Slots J. Herpesviruses in HIV-periodontitis. J Clin Periodontol 2001; 28: 96-102.
- 11. Leach CT, Pollock BH, McClain KL, Parmley RT, Murphy SB, Jenson HB. Human herpesvirus 6 and cytomegalovirus infections in children with human immunodeficiency virus infection and cancer. Pediatr Infect Dis J 2002 ;21: 125-32.
- 12. Karlı B, Önel M, Eraksoy H, Ağaçfidan A. İstanbul'da anti-HIV-1 pozitif hastalarda HHV-8 prevalansının araştırılması. Mikrobiyol Bul 2013; 47: 493-9
Abstract
Objective: The aim of this
study was to determine the frequency of HHV-6 DNA in patients with HIV positive
infection and HHV-6 variant type (variant A or variant B) in patients with HHV-6
DNA positive
Material and Method: Twenty
HIV-positive patients and 57 HIV negative healthy subjects were included in the
study. HHV-6 DNA was investigated by polymerase chain reaction (PCR) in the
patient and control groups. In cases with HHV-6 DNA positive, HHV-6 variant was
investigated with restriction fragment length polymorphism (RFLP). Statistical
analysis was performed using SPSS program, p≤0.05 was considered statistically
significant.
Results: HHV-6 DNA positivity was found
to be 45% (9/20) in HIV positive patients and 33.3% (19/57) in HIV negative
patients. Overall, the positivity rate was 36.3% (28/77). HHV-6 variant B was
detected in HIV positive patients, but variant A was not.
Conclusion: We believe that
further clinical studies involving more cases are needed to determine the
importance of HHV-6 seropositivity and variant types in HIV positive patients.
Keywords
References
- 1. Braun DK, Dominguez G, Pellet PE. Human Herpesvirus-6. Clin Mic Rev 1997; 10: 521-67.
- 2. Dolcetti R, Luca DD, Mİrandola P, et al. Frequent detection of Human Herpesvirus- 6 DNA in HIV associated lymphoadenopathy. Lancet 1994; 344: 543.
- 3. Fox J, Briggs M, Tedder RS. Antibody to human herpesvirus 6 in HIV-1 positive and negative homosexual men. Lancet1988; ii, 396-7.
- 4. Spira TJ, Bozeman LH, Sanderlin KC, et al. Lack of correlation between human herpesvirus-6 infection and the course of human immunodeficiency virus infection. J Infect Dis 1989; 161: 567-70.
- 5. Corbellino M, Lusso P, Gallo RC. Disseminated human herpesvirus 6 infection in AIDS. Lancet 1993; 342: 1242.
- 6. Lindquester GJ, Inoue N, Allen RD, et al. Restriction endonuclease mapping and molecular cloning of the Human Herpesvirus 6 variant B strain Z29 genome. Arch Virol1996; 141: 367-79.
- 7. Aubin JT, Collandre H, Candotti D, et al. Several groups among human herpesvirus 6 strains can be distinguished by southernblotting and polymerase chain reaction. J Clin Microbiol 1991; 29, 367-72.
- 8. Kositanont U, Wasi C, Wanprapar N, et al. Primary infection of human herpesvirus 6 in children with infection of human immunodeficiency virus type 1. J Infect Dis 1990; 180: 50-5.
- 9. Munawwar A, Singh S. Human herpesviruses as copathogens of HIV infection, Their role in HIV transmission, and disease progression. J Lab Physicians 2016; 8: 5-18.
- 10. Contreras A, Mardirossian A, Slots J. Herpesviruses in HIV-periodontitis. J Clin Periodontol 2001; 28: 96-102.
- 11. Leach CT, Pollock BH, McClain KL, Parmley RT, Murphy SB, Jenson HB. Human herpesvirus 6 and cytomegalovirus infections in children with human immunodeficiency virus infection and cancer. Pediatr Infect Dis J 2002 ;21: 125-32.
- 12. Karlı B, Önel M, Eraksoy H, Ağaçfidan A. İstanbul'da anti-HIV-1 pozitif hastalarda HHV-8 prevalansının araştırılması. Mikrobiyol Bul 2013; 47: 493-9
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Original Article |
| Authors | |
| Publication Date | January 15, 2020 |
| Published in Issue | Year 2020 Volume: 3 Issue: 1 |
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