Investigation of the effects of St. John’s wort (Hypericum perforatum) oil on the methotrexate-induced gastrointestinal mucosa injury and hepatotoxicity in mice
Abstract
St. John's wort oil is one of the herbal product which used for therapeutic purposes. To investigate the effects of St. John's Wort oil on irritated gastrointestinal mucosa and liver. The study is based on experimental animal trials. 21 healthy swiss albino mice were randomly divided into three groups: control group, group-2, group-3. The methotrexate (20 mg/kg) was given intraperitoneally as a single dose on the 1st day to the group-2 and group-3. St. John's Wort oil (4 ml/kg) was administered by gavages into the stomach daily for 7 days to the group-3. Following the necropsy, the gastrointestinal mucosa samples and the liver tissue samples were collected for histopathological evaluation. No pathology was found in the gastrointestinal mucosa and liver of control group. No pathology was found in the mice of group-3 in histopathological examination of the gastric mucosa. Significant hyperplasia and mononuclear cell infiltrations in lamina propria were observed in the mice of the group-2’s intestinal mucosa. The group-3 mice's intestinal mucosa was similar to control group's intestinal mucosa. In liver histopathology, mononuclear cell infiltrations and locally degenerative changes in the periportal area, vascular congestion and sinusoidal enlargement, thrombotic changes in the vessels, bile duct hyperplasia, megalocytosis in hepatocytes in some areas were observed in the livers of group-2 mice. Only a mild sinusoidal enlargement was noted in group-3's liver. According to the data we obtained in our study, St. John's Wort oil has protective and restorative effects especially on liver and intestinal mucosa if it is used consciously.
Thanks
We would like to thank Adana Veterinary Control Institute Directorate and staff for supporting our work.
References
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- 11. Sarris J. St. John's wort for the treatment of psychiatric disorders. Psychiatr Clin North Am. 2013;36(1):65-72.
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- 13. Albrecht K, Müller-Ladner U. Side effects and management of side effects of methotrexate in rheumatoid arthritis. Clin Exp Rheumatol. 2010;28:95-101.
- 14. Aksu Ö, Altınterim B. St. John’s Worth (Hypericum perforatum) and hypericin. Bilim ve Gençlik Derg. 2015;3(1):58-64.
- 15. Kurt H, Özbayer C, Değirmenci İ, Saadat SM, Üstüner MC, Köroğlu ZÖ, et al. Protective effects of Hypericum perforatum oil on indomethacin-induced gastric mucosal damage. Bozok Med J. 2016;6(3):62-8.
- 16. Yalçın S, Boğa Ö, Pekmezekmek AB. The role of St John's Wort (Hypericum perforatum) and orphanin interaction in depression treatment. Arşiv Kaynak Tarama Dergisi. 2015;24(4):531-541.
- 17. Teke HÜ, Özen M. Low-dose methotrexate induces pancytopenia in rheumatoid arthritis patients: are the risk factors predictors for physicians?. Turk J Phys Med Rehab. 2014;60:54-7.
Abstract
References
- 1. Kocaman N, Çolakoğlu N. Effects of repeated doses administration of methotrexate on rat liver tissue. Fırat Med J. 2013;18(3):141-145.
- 2. Kocaman N, Dabak DÖ. Investigation of the protective effects of silymarin on the methotrexate-induced hepatotoxicity in rats. Fırat Med J. 2019;33(2):67-72.
- 3. Armağan İ. The role of oxidative stress in methotrexate-induced damage in liver and kidney of rats. SDU Medical Faculty J. 2015;22(4):151-155.
- 4. Gaies E, Jebabli N, Trabelsi S, Salouage I, Charfi R, Lakhal M, et al. Methotrexate side effects. J Drug Metab Toxicol. 2012;3:125.
- 5. Wade SD, Yoshida EM, Carruthers MN, Weinblatt ME. Transient elastography for monitoring for hepatotoxicity in rheumatoid arthritis patients on long-term methotrexate. J Clin Rheumatol. 2018;10:1097.
- 6. West SG. Methotrexate hepatoxicity. Rheumatic diseases clinics of North America. 1997;23:883-915.
- 7. Olsen EA. The pharmacology of methotrexate. J Am Acad Dermatol. 1991;25:306-318.
- 8. Tsukada T, Nakano T, Miyata T, Sasaki S. Life-threatening gastrointestinal mucosal necrosis during methotrexate treatment for rheumatoid arthritis. Case Rep Gastroenterol. 2013;7:470-475.
- 9. Greeson JM, Sanford B, Monti DA. St. John's wort (Hypericum perforatum): a review of the current pharmacological, toxicological, and clinical literatüre. Psychopharmacology. 2001;153(4):402-14.
- 10. Hışıl Y, Şahin F, Omay SB. Composition of Hypericum perforatum L. and its medical importance. UHOD. 2005;15(4):212-218.
- 11. Sarris J. St. John's wort for the treatment of psychiatric disorders. Psychiatr Clin North Am. 2013;36(1):65-72.
- 12. Güven E, Erişgin Z, Tekelioğlu Y. Histopathological effects of shilajit on methotrexate-induced testicular damage in rats. Kocatepe Med J. 2017;18:1-6.
- 13. Albrecht K, Müller-Ladner U. Side effects and management of side effects of methotrexate in rheumatoid arthritis. Clin Exp Rheumatol. 2010;28:95-101.
- 14. Aksu Ö, Altınterim B. St. John’s Worth (Hypericum perforatum) and hypericin. Bilim ve Gençlik Derg. 2015;3(1):58-64.
- 15. Kurt H, Özbayer C, Değirmenci İ, Saadat SM, Üstüner MC, Köroğlu ZÖ, et al. Protective effects of Hypericum perforatum oil on indomethacin-induced gastric mucosal damage. Bozok Med J. 2016;6(3):62-8.
- 16. Yalçın S, Boğa Ö, Pekmezekmek AB. The role of St John's Wort (Hypericum perforatum) and orphanin interaction in depression treatment. Arşiv Kaynak Tarama Dergisi. 2015;24(4):531-541.
- 17. Teke HÜ, Özen M. Low-dose methotrexate induces pancytopenia in rheumatoid arthritis patients: are the risk factors predictors for physicians?. Turk J Phys Med Rehab. 2014;60:54-7.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | August 31, 2021 |
| Submission Date | May 24, 2021 |
| Published in Issue | Year 2021 Volume: 6 Issue: 2 |
