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DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS

Year 2003, Volume: 16 Issue: 3, 195 - 200, 03.12.2016

Abstract

Objective: The aim of the present study was to analyze the area of glutamate immunoreactive (-ir) dendritic spines in CA3 and dentate gyrus hilar regions in Genetic Absence Epilepsy Rats from Strasbourg (GAERS) hippocampus by electron microscopy in order to investigate possible glutamate-induced morphological changes in dendritic spines.
Methods: GAERS and non-epileptic control (NEC) rats were used. The brains were removed after perfusion fixation and CA3 and dentate hilar regions of the hippocampus were studied using immunogold electron microscopical methods. The area of dendritic spines was measured with the program NIH Image Analysis.
Results: There was no significant difference in the mean number of glutamate-ir spines making asymmetric synapses per mossy terminal in CA3 and dentate hilar regions compared to the control group. No significant difference in the area of
glutamate-ir dendritic spines making asymmetric synapses with mossy fiber terminals between NEC and GAERS groups was found.
Conclusion: Dendritic spines in the
hippocampus seem not to be affected in absence epilepsy, as opposed to those in temporal lobe epilepsy (TLE). This may be due to differences in pathogenetic mechanisms between TLE and absence epilepsy.
Key Words: Hippocampus, GAERS,
Ultrastructure, Dendritic spine

References

  • Danober L, Deransart C, Depaulis A, Vergnes M, Marescaux C. Pathophysiological mechanisms of genetic absence epilepsy in the rat. Prog Heurobiol 1998;55:27-57.
  • Marescaux C, Vergnes M. Genetic absence epilepsy in rats from Strasbourg (GAERS). Ital J Meurol Sci 1995; 16:115-118.
  • Richards DA, Morrone LA, Bowery HG. Hippocampal extracellular amino acids and EEG spectral analysis in a genetic rat model of absence epilepsy. Heuropharmacology 2000;39:2435-2441.
  • Hehlig A, Vergnes M, Boyet S, Marescaux C. Local cerebral glucose utilization in adult and immature GAERS. Epilepsy Res 1998:32:206- 212.
  • SegaI M. Dendritic spines for neuroprotection: a hypothesis. Trends Heurosci ¡995,18:468- 471.
  • Harris RM, Rater SB. Dendritic spines: cellular specializations imparting both stability and flexibility to synaptic function. Annu Rev Heurosci 1994,17:341-371.
  • Choi DW. Glutamate receptors and the induction of excitotoxic neuronal death. Prog Brain Res 1994,100:47-51.
  • Segal M, Rorkotian E, Murphy DD. Dendritic spine formation and prunning: common cellular mechanisms? Trends Heurosci 2000;23:53-57.
  • Isokawa M. Preservation of dendrites with the presence of reorganized mossy fiber collaterals in hippocampal dentate granule cells in patients with temporal lobe epilepsy. Brain Res 1997;744:339-343.
  • Phelps S, Mitchell J, Wheal HV. Changes to synaptic ultrastructure in field CA1 of the rat hippocampus following intracerebroventricular injection of kainic acid. Heuroscience 1991; 40:687-699.
  • Pyapali GR, Turner DA. Denervation-induced dendritic alterations in CA1 pyramidal cells following kainic acid hippocampal lesions in rats. Brain Res 1994;652:279-290.
  • Isokawa M, Mello LEAM. HMDA receptor- mediated excitability in dendritically deformed dentate granule cells in pilocarpine-treated rats. Heurosci Lett 1991; 129:609-613.
  • Isokawa M. Decreased time constant in hippocampal dentate granule cells in pilocarpine-treated rafs with progressive seizure frequencies. Brain Res 1996;718:1901-1908.
  • Serap Şirvancı, et al.
  • Colling SB, Man WD-C, Draguhn A, Jefferys JOR. Dendritic shrinkage and dye-coupling between rat hippocampal CA1 pyramidal cells in the tetanus toxin model of epilepsy. Brain Res 1996;741:38-43.
  • Jiang M, Lee CL, Smith KL, Swann JW. Spine loss and other persistent alterations of hippocampal pyramidal cell dendrites in a model of early-onset epilepsy. J Fieurosci 1998,18:8356-8368.
  • Spigelman I, Yan X-X, Obenaus A, Lee EY-S, Wasterlain CO, Ribak CE. Dentate granule cells form novel basal dendrites in a rat model of temporal lobe epilepsy, neuroscience 1998;86:109-120.
  • Suzuki F, Junier M-P, Guilhem D, Sorensen J- C, Onteniente B. Morphogenetic effect of kainate on adult hippocampal neurons associated with a prolonged expression of brain-derived neurotrophic factor, neuroscience 1995;64:665-674.
  • Von Campe G, Spencer DD, De Lanorelle nc. Morphology of dentate granule cells in the human epileptogenic hippocampus, hippocampus 1997;7:472-488.
  • Şirvancı S, Meshul CR, Onat F, Şan T. Immunocytochemical analysis of glutamate and GABA in hippocampus of genetic absence epilepsy rats (GAERS). Brain Res 2003:988: 180-188.
  • lialpain S, flipolito A, Saffer L. Regulation of F- actin stability in dendritic spines by glutamate receptors and calcineurin. J Meurosci 1998; 18:9835-9844.
  • Blümcke I, Zuschratter W, Schewe J-C, et at. Cellular pathology of hilar neurons in Ammon's horn sclerosis. J Comp Meurol 1999;414:437-453.
  • Vaquero J, Oya S, Cabezudo JM, Bravo G. Morphological study of human epileptic dendrites, neurosurgery 1982;10:720-724.
Year 2003, Volume: 16 Issue: 3, 195 - 200, 03.12.2016

Abstract

References

  • Danober L, Deransart C, Depaulis A, Vergnes M, Marescaux C. Pathophysiological mechanisms of genetic absence epilepsy in the rat. Prog Heurobiol 1998;55:27-57.
  • Marescaux C, Vergnes M. Genetic absence epilepsy in rats from Strasbourg (GAERS). Ital J Meurol Sci 1995; 16:115-118.
  • Richards DA, Morrone LA, Bowery HG. Hippocampal extracellular amino acids and EEG spectral analysis in a genetic rat model of absence epilepsy. Heuropharmacology 2000;39:2435-2441.
  • Hehlig A, Vergnes M, Boyet S, Marescaux C. Local cerebral glucose utilization in adult and immature GAERS. Epilepsy Res 1998:32:206- 212.
  • SegaI M. Dendritic spines for neuroprotection: a hypothesis. Trends Heurosci ¡995,18:468- 471.
  • Harris RM, Rater SB. Dendritic spines: cellular specializations imparting both stability and flexibility to synaptic function. Annu Rev Heurosci 1994,17:341-371.
  • Choi DW. Glutamate receptors and the induction of excitotoxic neuronal death. Prog Brain Res 1994,100:47-51.
  • Segal M, Rorkotian E, Murphy DD. Dendritic spine formation and prunning: common cellular mechanisms? Trends Heurosci 2000;23:53-57.
  • Isokawa M. Preservation of dendrites with the presence of reorganized mossy fiber collaterals in hippocampal dentate granule cells in patients with temporal lobe epilepsy. Brain Res 1997;744:339-343.
  • Phelps S, Mitchell J, Wheal HV. Changes to synaptic ultrastructure in field CA1 of the rat hippocampus following intracerebroventricular injection of kainic acid. Heuroscience 1991; 40:687-699.
  • Pyapali GR, Turner DA. Denervation-induced dendritic alterations in CA1 pyramidal cells following kainic acid hippocampal lesions in rats. Brain Res 1994;652:279-290.
  • Isokawa M, Mello LEAM. HMDA receptor- mediated excitability in dendritically deformed dentate granule cells in pilocarpine-treated rats. Heurosci Lett 1991; 129:609-613.
  • Isokawa M. Decreased time constant in hippocampal dentate granule cells in pilocarpine-treated rafs with progressive seizure frequencies. Brain Res 1996;718:1901-1908.
  • Serap Şirvancı, et al.
  • Colling SB, Man WD-C, Draguhn A, Jefferys JOR. Dendritic shrinkage and dye-coupling between rat hippocampal CA1 pyramidal cells in the tetanus toxin model of epilepsy. Brain Res 1996;741:38-43.
  • Jiang M, Lee CL, Smith KL, Swann JW. Spine loss and other persistent alterations of hippocampal pyramidal cell dendrites in a model of early-onset epilepsy. J Fieurosci 1998,18:8356-8368.
  • Spigelman I, Yan X-X, Obenaus A, Lee EY-S, Wasterlain CO, Ribak CE. Dentate granule cells form novel basal dendrites in a rat model of temporal lobe epilepsy, neuroscience 1998;86:109-120.
  • Suzuki F, Junier M-P, Guilhem D, Sorensen J- C, Onteniente B. Morphogenetic effect of kainate on adult hippocampal neurons associated with a prolonged expression of brain-derived neurotrophic factor, neuroscience 1995;64:665-674.
  • Von Campe G, Spencer DD, De Lanorelle nc. Morphology of dentate granule cells in the human epileptogenic hippocampus, hippocampus 1997;7:472-488.
  • Şirvancı S, Meshul CR, Onat F, Şan T. Immunocytochemical analysis of glutamate and GABA in hippocampus of genetic absence epilepsy rats (GAERS). Brain Res 2003:988: 180-188.
  • lialpain S, flipolito A, Saffer L. Regulation of F- actin stability in dendritic spines by glutamate receptors and calcineurin. J Meurosci 1998; 18:9835-9844.
  • Blümcke I, Zuschratter W, Schewe J-C, et at. Cellular pathology of hilar neurons in Ammon's horn sclerosis. J Comp Meurol 1999;414:437-453.
  • Vaquero J, Oya S, Cabezudo JM, Bravo G. Morphological study of human epileptic dendrites, neurosurgery 1982;10:720-724.
There are 23 citations in total.

Details

Journal Section Original Research
Authors

Serap Şirvancı

Şükrü Midillioğlu This is me

Charles Meshul This is me

Filiz Onat This is me

Tangül Şan This is me

Publication Date December 3, 2016
Published in Issue Year 2003 Volume: 16 Issue: 3

Cite

APA Şirvancı, S., Midillioğlu, Ş., Meshul, C., Onat, F., et al. (2016). DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS. Marmara Medical Journal, 16(3), 195-200.
AMA Şirvancı S, Midillioğlu Ş, Meshul C, Onat F, Şan T. DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS. Marmara Med J. March 2016;16(3):195-200.
Chicago Şirvancı, Serap, Şükrü Midillioğlu, Charles Meshul, Filiz Onat, and Tangül Şan. “DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS”. Marmara Medical Journal 16, no. 3 (March 2016): 195-200.
EndNote Şirvancı S, Midillioğlu Ş, Meshul C, Onat F, Şan T (March 1, 2016) DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS. Marmara Medical Journal 16 3 195–200.
IEEE S. Şirvancı, Ş. Midillioğlu, C. Meshul, F. Onat, and T. Şan, “DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS”, Marmara Med J, vol. 16, no. 3, pp. 195–200, 2016.
ISNAD Şirvancı, Serap et al. “DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS”. Marmara Medical Journal 16/3 (March 2016), 195-200.
JAMA Şirvancı S, Midillioğlu Ş, Meshul C, Onat F, Şan T. DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS. Marmara Med J. 2016;16:195–200.
MLA Şirvancı, Serap et al. “DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS”. Marmara Medical Journal, vol. 16, no. 3, 2016, pp. 195-00.
Vancouver Şirvancı S, Midillioğlu Ş, Meshul C, Onat F, Şan T. DENDRITIC SPINES IN THE HIPPOCAMPUS OF GENETIC ABSENCE EPILEPSY RATS (GAERS): AN ULTRASTRUCTURAL OUANTITATIVE ANALYSIS. Marmara Med J. 2016;16(3):195-200.