Research Article
Polikistik Over Sendromu Olan Adölesanlarda Hipoksiyle İndüklenen Faktör (HIF) 1-Alfanın Değerlendirilmesi ve Önemi
Abstract
Adölesan polikistik over sendromu (PCOS) tanısında, serum hipoksiyle indüklenen faktör (HIF)-1 alfanın yerinin, klinik öneminin ve bir biyobelirteç olarak kullanılıp kullanılamayacağının araştırılması amaçlanmıştır. Üçüncü Basamak Kadın Sağlığı Eğitim ve Araştırma Hastanesinde, yaşları 15 ve 22 arasında değişen 30 PCOS’lu hasta, 30 sağlıklı adölesan ve genç yetişkin kadın çalışmaya dahil edildi. Her hastada genel fizik muayene, pelvik muayene ve ultrasonografik değerlendirme yapıldı. Serum açlık glukoz ve insulin seviyesi, lipid profili, HIF-1 alfa seviyesi hastaların esas parametreleri olarak kayıt edildi. Gruplar arasında serum HIF-1 alfa seviyeleri arasında istatistiksel olarak fark bulunmadı. PCOS hasta grubunda ortalama HIF-1 alfa seviyesi, sağlıklı popülasyona göre daha yüksek bulunmasına ragmen bu fark istatistiksel olarak anlamlı değildi. Bel/kalça çevresi oranı, serum LH seviyesi, LH/FSH oranı ve serum androjen seviyeleri PCOS’lu hastalarda kontrollere göre anlamlı olarak yüksek bulundu. Çalışmaya az sayıda hasta dahil edilmesi ve hasta grubunun PCOS semptom ve bulgularının henüz netleşmediği bir dönem olan adölesan yaş aralığını kapsaması, çalışmamızın limitasyonlarını oluşturmaktadır ve olasılıkla çalışma bulgularımızı etkilemiştir. PCOS tanısının konulmasında HIF-1 alfanın yerinin değerlendirilmesi için daha geniş hasta grupları içeren çalışmalara ihtiyaç vardır.
Keywords
References
- 1. Fauser BC, Tarlatzis BC, Rebar RW, et al. Consensus on women's health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group. Fertil steril. 2012;97(1):28-38.e25.
- 2. Hart R, Doherty DA. The potential implications of a PCOS diagnosis on a woman's long-term health using data linkage. J Clin Endocrinol Metab. 2015;100(3):911-9.
- 3. Twig G, Yaniv G, Levine H, et al. Body-mass index in 2.3 million adolescents and cardiovascular death in adulthood. N Engl J Med. 2016;374(25):2430-40.
- 4. Macut D, Bjekic-Macut J, Savic-Radojevic A. Dyslipidemia and oxidative stress in PCOS. Front Horm Res. 2013;40:51-63.
- 5. Murri M, Luque-Ramirez M, Insenser M, et al. Circulating markers of oxidative stress and polycystic ovary syndrome (PCOS): a systematic review and meta-analysis. Hum Reprod Update. 2013;19(3):268-88.
- 6. Lai Q, Xiang W, Li Q, et al. Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with polycystic ovary syndrome. Front Med. 2018;12(5):518-24. 7. Avni R, Cohen B, Neeman M. Hypoxic stress and cancer: imaging the axis of evil in tumor metastasis. NMR Biomed. 2011;24(6):569-81.
- 8. Bahadori B, Uitz E, Mayer A, et al. Polymorphisms of the hypoxia-inducible factor 1 gene and peripheral artery disease. Vasc Med. 2010;15(5):371-4.
- 9. Agani FH, Pichiule P, Chavez JC, et al. The role of mitochondria in the regulation of hypoxia-inducible factor 1 expression during hypoxia. J Biol Chem. 2000;275(46):35863-7.
- 10. Chandel NS, McClintock DS, Feliciano CE, et al. Reactive oxygen species generated at mitochondrial complex III stabilize hypoxia-inducible factor-1alpha during hypoxia: a mechanism of O2 sensing. J Biol Chem. 2000;275(33):25130-8.
- 11. Guzy RD, Hoyos B, Robin E, et al. Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing. Cell Metab. 2005;1(6):401-8.
- 12. Schroedl C, McClintock DS, Budinger GR, et al. Hypoxic but not anoxic stabilization of HIF-1alpha requires mitochondrial reactive oxygen species. Am J Physiol Lung Cell Mol Physiol. 2002;283(5):L922-31.
- 13. Ma Z, Zhang Y, Li Q, et al. Resveratrol improves alcoholic fatty liver disease by downregulating HIF-1alpha expression and mitochondrial ROS production. PLoS One. 2017;12(8):e0183426. 14. Coimbra-Costa D, Alva N, Duran M, et al. Oxidative stress and apoptosis after acute respiratory hypoxia and reoxygenation in rat brain. Redox Biol. 2017;12:216-25.
- 15. Venturoli S, Porcu E, Fabbri R, et al. Longitudinal change of sonographic ovarian aspects and endocrine parameters in irregular cycles of adolescence. Pediatr Res. 1995;38(6):974-80.
- 16. Pasquali R, Casimirri F, Venturoli S, et al. Body fat distribution has weight-independent effects on clinical, hormonal, and metabolic features of women with polycystic ovary syndrome. Metabolism. 1994;43(6):706-13.
- 17. Ates S, Aydin S, Ozcan P, et al. Clinical and metabolic characteristics of Turkish adolescents with polycystic ovary syndrome. J Obstet Gynaecol. 2018;38(2):236-40.
- 18. Witchel SF, Oberfield S, Rosenfield RL, et al. The diagnosis of polycystic ovary syndrome during adolescence. Horm Res Paediatr. 2015;83(6):376-89.
- 19. Tosi F, Fiers T, Kaufman JM, et al. Implications of androgen assay accuracy in the phenotyping of women with polycystic ovary syndrome. The Journal of clinical endocrinology and metabolism. 2016;101(2):610-8.
Evaluation and Importance of Hypoxia Inducible Factor (HIF)-1-Alpha in Adolescents with Polycystic Ovary Syndrome
Abstract
It was aimed at investigating the place of serum hypoxia inducible factor (HIF)-1 alpha level in the diagnosis of adolescent polycystic ovary syndrome (PCOS), its clinical significance and whether or not it can be used as a biomarker. 30 patients with PCOS and 30 healthy adolescent and young adult women with ages ranging between 15 and 22 were recruited in a grade III Women’s Health Education and Research Hospital. A general physical examination, pelvic examination and ultrasonographic evaluation was carried out on each patient. Serum fasting glucose and insulin levels, lipid profile, HIF-1 alpha levels were recorded as the main parameters of patients. No statistically significant differences were found in serum HIF-1 alpha levels between groups. While the mean HIF-1 alpha levels in PCOS patients was higher as compared to the healthy population, this difference was not statistically significant. Waist/hip circumference ratio, serum LH level, LH/FSH ratio and serum androgen levels in patients with PCOS were significantly higher as compared to control cases. The small number of patients included in the study and the age range covering the adolescence, which is the period that PCOS symptoms and findings have not become clear yet constitute the limitations of our study, and it is possible that they have affected out study results. Place of HIF-1 alpha level in the diagnosis of PCOS must be supported with studies including larger patient groups.
Keywords
References
- 1. Fauser BC, Tarlatzis BC, Rebar RW, et al. Consensus on women's health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group. Fertil steril. 2012;97(1):28-38.e25.
- 2. Hart R, Doherty DA. The potential implications of a PCOS diagnosis on a woman's long-term health using data linkage. J Clin Endocrinol Metab. 2015;100(3):911-9.
- 3. Twig G, Yaniv G, Levine H, et al. Body-mass index in 2.3 million adolescents and cardiovascular death in adulthood. N Engl J Med. 2016;374(25):2430-40.
- 4. Macut D, Bjekic-Macut J, Savic-Radojevic A. Dyslipidemia and oxidative stress in PCOS. Front Horm Res. 2013;40:51-63.
- 5. Murri M, Luque-Ramirez M, Insenser M, et al. Circulating markers of oxidative stress and polycystic ovary syndrome (PCOS): a systematic review and meta-analysis. Hum Reprod Update. 2013;19(3):268-88.
- 6. Lai Q, Xiang W, Li Q, et al. Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with polycystic ovary syndrome. Front Med. 2018;12(5):518-24. 7. Avni R, Cohen B, Neeman M. Hypoxic stress and cancer: imaging the axis of evil in tumor metastasis. NMR Biomed. 2011;24(6):569-81.
- 8. Bahadori B, Uitz E, Mayer A, et al. Polymorphisms of the hypoxia-inducible factor 1 gene and peripheral artery disease. Vasc Med. 2010;15(5):371-4.
- 9. Agani FH, Pichiule P, Chavez JC, et al. The role of mitochondria in the regulation of hypoxia-inducible factor 1 expression during hypoxia. J Biol Chem. 2000;275(46):35863-7.
- 10. Chandel NS, McClintock DS, Feliciano CE, et al. Reactive oxygen species generated at mitochondrial complex III stabilize hypoxia-inducible factor-1alpha during hypoxia: a mechanism of O2 sensing. J Biol Chem. 2000;275(33):25130-8.
- 11. Guzy RD, Hoyos B, Robin E, et al. Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing. Cell Metab. 2005;1(6):401-8.
- 12. Schroedl C, McClintock DS, Budinger GR, et al. Hypoxic but not anoxic stabilization of HIF-1alpha requires mitochondrial reactive oxygen species. Am J Physiol Lung Cell Mol Physiol. 2002;283(5):L922-31.
- 13. Ma Z, Zhang Y, Li Q, et al. Resveratrol improves alcoholic fatty liver disease by downregulating HIF-1alpha expression and mitochondrial ROS production. PLoS One. 2017;12(8):e0183426. 14. Coimbra-Costa D, Alva N, Duran M, et al. Oxidative stress and apoptosis after acute respiratory hypoxia and reoxygenation in rat brain. Redox Biol. 2017;12:216-25.
- 15. Venturoli S, Porcu E, Fabbri R, et al. Longitudinal change of sonographic ovarian aspects and endocrine parameters in irregular cycles of adolescence. Pediatr Res. 1995;38(6):974-80.
- 16. Pasquali R, Casimirri F, Venturoli S, et al. Body fat distribution has weight-independent effects on clinical, hormonal, and metabolic features of women with polycystic ovary syndrome. Metabolism. 1994;43(6):706-13.
- 17. Ates S, Aydin S, Ozcan P, et al. Clinical and metabolic characteristics of Turkish adolescents with polycystic ovary syndrome. J Obstet Gynaecol. 2018;38(2):236-40.
- 18. Witchel SF, Oberfield S, Rosenfield RL, et al. The diagnosis of polycystic ovary syndrome during adolescence. Horm Res Paediatr. 2015;83(6):376-89.
- 19. Tosi F, Fiers T, Kaufman JM, et al. Implications of androgen assay accuracy in the phenotyping of women with polycystic ovary syndrome. The Journal of clinical endocrinology and metabolism. 2016;101(2):610-8.
There are 17 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Surgery |
| Journal Section | Original Article |
| Authors | |
| Publication Date | August 24, 2022 |
| Submission Date | June 3, 2021 |
| Published in Issue | Year 2022 Volume: 9 Issue: 2 |
