Anti-Angiogenic and Oxidant Effects of Monosodium Glutamate at Different Concentrations in Chorioallantoic Membrane Model
Abstract
Monosodium glutamate (MSG) is a flavor-enhancing food additive. MSG exposure is rising day by day because of the high commercial food consumption. MSG exposure causes damage to various tissues and organs. The aim of this study is to investigate the effects of MSG on angiogenesis and oxidant-antioxidant balance. Three different concentrations of MSG (10-4 M, 10-5 M, and 10-6 M), control, and the bevacizumab (10-6 M) were prepared and placed on the chorioallantoic membrane (CAM) of the embryos. Albumen was taken from the embryos before and after the experiment. Angiogenesis was investigated through the window that was opened on the eggshell. Angiogenesis was found to be normal in the control and 10-6 M MSG group (average score: 0.3). Anti-angiogenic effects were moderate in the 10-5 M MSG group (average score: 0.5) and in the 10-4 M MSG group (average score: 0.7), and strong in the bevacizumab group (average score: 1.1). According to our results, MSG shows anti-angiogenic properties in higher doses. MSG increased oxidative stress. According to the results of our research, it is seen that MSG inhibits angiogenesis in a dose-dependent manner in the CAM model and may cause an increase in oxidative damage by disrupting the oxidant-antioxidant balance. Since no previous study has been found in the literature regarding the effects of MSG on angiogenesis and oxidant-antioxidant balance in the CAM model, we think our results will fill an important gap in the literature.
Supporting Institution
-
Project Number
-
Thanks
-
References
- 1. Chakraborty SP. Patho-physiological and toxicological aspects of monosodium glutamate. Toxicol Mech Methods. 2019;29(6):389-96.
- 2. Vorhees CV. A test of dietary monosodium glutamate developmental neurotoxicity in rats: a reappraisal. Ann Nutr Metab. 2018;73(5):36-42.
- 3. Olney JW. Brain lesions, obesity, and other disturbances in mice treated with monosodium glutamate. Science. 1969;164(3880):719-21.
- 4. Hajihasani MM, Soheili V, Zirak MR, et al. Natural products as safeguards against monosodium glutamate-induced toxicity. Iran J Basic Med Sci. 2020;23(4):416-30.
- 5. Gültekin F, Nazıroğlu M, Savaş HB, et al. Calorie restriction protects against apoptosis, mitochondrial oxidative stress and increased calcium signaling through inhibition of TRPV1 channel in the hippocampus and dorsal root ganglion of rats. Metab Brain Dis. 2018;33(5):1761-74.
- 6. Temelli B, Yetkin Ay Z, Savaş HB, et al. Circulation levels of acute phase proteins pentraxin 3 and serum amyloid A in atherosclerosis have correlations with periodontal inflamed surface area. J Appl Oral Sci. 2018;26:e20170322.
- 7. Savran M, Ozmen O, Erzurumlu Y, et al. The impact of prophylactic lacosamide on lps-induced neuroinflammation in aged rats. Inflammation. 2019;42(5):1913-24.
- 8. Ribatti D. Chicken chorioallantoic membrane angiogenesis model. Methods Mol Biol. 2012;843:47-57.
- 9. Ribatti D. The chick embryo chorioallantoic membrane (CAM) assay. Reprod Toxicol. 2017;70:97-101.
- 10. Yavuz C, Caliskan A, Karahan O, et al. Investigation of the antiangiogenic behaviors of rivaroxaban and low molecular weight heparins. Blood Coagul Fibrinolysis. 2014;25(4):303-8.
- 11. Amagase K, Ochi A, Kojo A, et al. New therapeutic strategy for amino acid medicine: prophylactic and healing promoting effect of monosodium glutamate against NSAID-induced enteropathy. J Pharmacol Sci. 2012;118(2):131-7.
- 12. Amagase K, Kimura Y, Wada A, et al. Prophylactic effect of monosodium glutamate on NSAID-induced enteropathy in rats. Curr Pharm Des. 2014;20(16):2783-90.
- 13. Wahyuni DE, Situmorang C, WisnuBarlianto C, et al. Combination of vitamin C and E modulated monosodium glutamate-induced endometrial toxicity in female Wistar rat. Asian Pac J Reprod. 2014;3:106-9.
- 14. Dal S, Arslan S, Nurol NK, et al. Monosodium glutamate below the neurotoxic doses has no cytotoxic effect on Mouse mesenchymal stem cells. Cumhur Medical J. 2017;39(3):525-30.
- 15. Castañeda-Cabral JL, Beas-Zarate C, Gudiño-Cabrera G, et al. Glutamate neonatal excitotoxicity modifies VEGF-A, VEGF-B, VEGFR-1 and VEGFR-2 protein expression profiles during postnatal development of the cerebral cortex and hippocampus of male rats. J Mol Neurosci. 2017;63(1):17-27.
- 16. Savas HB, Kara E. The oxidative stress and antioxidants in scientific research. Turk J Health S. 2021;2(3):28-30.
- 17. Cankara FN, Özmen Ö, Savaş HB, et al. Gastroprotective effect of tarantula cubensis extract in the indomethacin-induced peptic ulcer model in rats. Acta Med Alanya 2020;4(3):278-84.
- 18. Savas HB, Gultekin F, Ciris İM. Positive effects of meal frequency and calorie restriction on antioxidant systems in rats. North Clin Istanbul. 2017;4(2):109–16.
- 19. Cuce G, Canbaz HT, Sozen ME, et al. Vitamin E and selenium treatment of monocrotaline induced hepatotoxicity in rats. Biotech Histochem. 2017;92(1):59-67.
- 20. Seflek HN, Kalkan S, Cuce G, et al. Effects of nigella sativa oil on ovarian volume, oxidant systems, XIAP and NF-kB expression in an experimental model of diabetes. Biotech Histochem. 2019;94(5):325-33.
- 21. Erdem D, Savas HB, Erdem N, et al. Ischemia modified albumin as a new marker for diagnosis of early pregnancy losses. Int J Acad Med Pharm. 2020;2(3):222-7.
- 22. Savas HB, Sayar E. Oxidant antioxidant balance and trace elements in children with functional dyspepsia. Turkiye Klinikleri J Med Sci. 2021;41(1):70-9.
- 23. Savas HB, Sayar E, Kara T. Thiol disulfide balance oxidative stress and paraoxonase 1 activities in children and adolescents aged 6-16 years with specific learning disorders. Electron J Gen Med. 2021;18(3): em290.
- 24. Etli M, Savas HB. Ischemia modified albumin as a novel biochemical indicator in peripheral artery patients. J Clin Exp Invest. 2021;3(12):em00774.
- 25. Savas HB, Etli M. Paraoxonase 1 activity as a new biochemical marker in the diagnosis of peripheral arterial disease. Turk J Clin Lab. 2021;12(1):29-32.
- 26. Savas HB, Erdem D. Paraoxonase 1 activities in first trimester miscarriages. Jinekololoji Neonatoloji Tıp Derg. 2021;18(2):770-5.
- 27. Etli M, Karahan O, Akkaya Ö, et al. Cilostazol induces angiogenesis and regulates oxidative stress in a dose-dependent manner: A chorioallantoic membrane study. Turk Gogus Kalp Damar Cerrahisi Derg. 2021;29(4):449-56.
Farklı Konsantrasyonlardaki Monosodyum Glutamatın Koryoallantoik Membran Modelinde Anti-Anjiyojenik ve Oksidan Etkileri
Abstract
Monosodyum glutamat (MSG), lezzet artırıcı bir gıda katkı maddesidir. İşlenmiş gıda tüketimindeki yükselişe bağlı olarak MSG maruziyeti her geçen gün artmaktadır. MSG maruziyeti çeşitli doku ve organlara zarar vermektedir. Bu çalışmanın amacı, MSG'nin anjiyogenez ve oksidan-antioksidan dengesi üzerindeki etkilerini araştırmaktır. Üç farklı konsantrasyonda MSG (10-4 M, 10-5 M ve 10-6 M), kontrol ve bevasizumab (10-6 M) hazırlanıp, embriyoların korioallantoik membranına (CAM) yerleştirildi. Deneyden önce ve sonra tavuk yumurtalarından sıvı alındı. Yumurta kabuğu üzerinde açılan pencereden anjiyogenez araştırıldı. Kontrol ve 10-6 M MSG grubunda (ortalama skor: 0.3) anjiyogenez normal bulundu. Anti-anjiyojenik etkilerin, 10-5 M MSG grubunda (ortalama puan: 0.5) ve 10-4 M MSG grubunda (ortalama puan: 0.7) orta düzeyde ve bevasizumab grubunda (ortalama puan: 1.1) güçlü olduğu tespit edildi. Sonuçlarımıza göre MSG daha yüksek dozlarda antianjiyogenik özellikler göstermektedir. Araştırmamızın sonuçlarına göre MSG'nin CAM modelinde anjiyogenezi doza bağımlı bir şekilde inhibe ettiği ve oksidan-antioksidan dengesini bozarak oksidatif hasarda artışa neden olabileceği görülmektedir. MSG'nin CAM modelinde anjiyogenez ve oksidan-antioksidan denge üzerindeki etkilerine ilişkin literatürde daha önce yapılmış bir çalışma tespit edilemediği için araştırma sonuçlarımızın literatürdeki önemli bir eksikliği gidereceğini düşünmekteyiz.
Project Number
-
References
- 1. Chakraborty SP. Patho-physiological and toxicological aspects of monosodium glutamate. Toxicol Mech Methods. 2019;29(6):389-96.
- 2. Vorhees CV. A test of dietary monosodium glutamate developmental neurotoxicity in rats: a reappraisal. Ann Nutr Metab. 2018;73(5):36-42.
- 3. Olney JW. Brain lesions, obesity, and other disturbances in mice treated with monosodium glutamate. Science. 1969;164(3880):719-21.
- 4. Hajihasani MM, Soheili V, Zirak MR, et al. Natural products as safeguards against monosodium glutamate-induced toxicity. Iran J Basic Med Sci. 2020;23(4):416-30.
- 5. Gültekin F, Nazıroğlu M, Savaş HB, et al. Calorie restriction protects against apoptosis, mitochondrial oxidative stress and increased calcium signaling through inhibition of TRPV1 channel in the hippocampus and dorsal root ganglion of rats. Metab Brain Dis. 2018;33(5):1761-74.
- 6. Temelli B, Yetkin Ay Z, Savaş HB, et al. Circulation levels of acute phase proteins pentraxin 3 and serum amyloid A in atherosclerosis have correlations with periodontal inflamed surface area. J Appl Oral Sci. 2018;26:e20170322.
- 7. Savran M, Ozmen O, Erzurumlu Y, et al. The impact of prophylactic lacosamide on lps-induced neuroinflammation in aged rats. Inflammation. 2019;42(5):1913-24.
- 8. Ribatti D. Chicken chorioallantoic membrane angiogenesis model. Methods Mol Biol. 2012;843:47-57.
- 9. Ribatti D. The chick embryo chorioallantoic membrane (CAM) assay. Reprod Toxicol. 2017;70:97-101.
- 10. Yavuz C, Caliskan A, Karahan O, et al. Investigation of the antiangiogenic behaviors of rivaroxaban and low molecular weight heparins. Blood Coagul Fibrinolysis. 2014;25(4):303-8.
- 11. Amagase K, Ochi A, Kojo A, et al. New therapeutic strategy for amino acid medicine: prophylactic and healing promoting effect of monosodium glutamate against NSAID-induced enteropathy. J Pharmacol Sci. 2012;118(2):131-7.
- 12. Amagase K, Kimura Y, Wada A, et al. Prophylactic effect of monosodium glutamate on NSAID-induced enteropathy in rats. Curr Pharm Des. 2014;20(16):2783-90.
- 13. Wahyuni DE, Situmorang C, WisnuBarlianto C, et al. Combination of vitamin C and E modulated monosodium glutamate-induced endometrial toxicity in female Wistar rat. Asian Pac J Reprod. 2014;3:106-9.
- 14. Dal S, Arslan S, Nurol NK, et al. Monosodium glutamate below the neurotoxic doses has no cytotoxic effect on Mouse mesenchymal stem cells. Cumhur Medical J. 2017;39(3):525-30.
- 15. Castañeda-Cabral JL, Beas-Zarate C, Gudiño-Cabrera G, et al. Glutamate neonatal excitotoxicity modifies VEGF-A, VEGF-B, VEGFR-1 and VEGFR-2 protein expression profiles during postnatal development of the cerebral cortex and hippocampus of male rats. J Mol Neurosci. 2017;63(1):17-27.
- 16. Savas HB, Kara E. The oxidative stress and antioxidants in scientific research. Turk J Health S. 2021;2(3):28-30.
- 17. Cankara FN, Özmen Ö, Savaş HB, et al. Gastroprotective effect of tarantula cubensis extract in the indomethacin-induced peptic ulcer model in rats. Acta Med Alanya 2020;4(3):278-84.
- 18. Savas HB, Gultekin F, Ciris İM. Positive effects of meal frequency and calorie restriction on antioxidant systems in rats. North Clin Istanbul. 2017;4(2):109–16.
- 19. Cuce G, Canbaz HT, Sozen ME, et al. Vitamin E and selenium treatment of monocrotaline induced hepatotoxicity in rats. Biotech Histochem. 2017;92(1):59-67.
- 20. Seflek HN, Kalkan S, Cuce G, et al. Effects of nigella sativa oil on ovarian volume, oxidant systems, XIAP and NF-kB expression in an experimental model of diabetes. Biotech Histochem. 2019;94(5):325-33.
- 21. Erdem D, Savas HB, Erdem N, et al. Ischemia modified albumin as a new marker for diagnosis of early pregnancy losses. Int J Acad Med Pharm. 2020;2(3):222-7.
- 22. Savas HB, Sayar E. Oxidant antioxidant balance and trace elements in children with functional dyspepsia. Turkiye Klinikleri J Med Sci. 2021;41(1):70-9.
- 23. Savas HB, Sayar E, Kara T. Thiol disulfide balance oxidative stress and paraoxonase 1 activities in children and adolescents aged 6-16 years with specific learning disorders. Electron J Gen Med. 2021;18(3): em290.
- 24. Etli M, Savas HB. Ischemia modified albumin as a novel biochemical indicator in peripheral artery patients. J Clin Exp Invest. 2021;3(12):em00774.
- 25. Savas HB, Etli M. Paraoxonase 1 activity as a new biochemical marker in the diagnosis of peripheral arterial disease. Turk J Clin Lab. 2021;12(1):29-32.
- 26. Savas HB, Erdem D. Paraoxonase 1 activities in first trimester miscarriages. Jinekololoji Neonatoloji Tıp Derg. 2021;18(2):770-5.
- 27. Etli M, Karahan O, Akkaya Ö, et al. Cilostazol induces angiogenesis and regulates oxidative stress in a dose-dependent manner: A chorioallantoic membrane study. Turk Gogus Kalp Damar Cerrahisi Derg. 2021;29(4):449-56.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Original Article |
| Authors | |
| Project Number | - |
| Publication Date | August 21, 2023 |
| Submission Date | June 29, 2022 |
| Published in Issue | Year 2023 Volume: 10 Issue: 2 |
