PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS

Gülşah Mersin; Ünsal Açıkel

doi:10.23902/trkjnat.817451

Research Article

PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS

Year 2021, Volume: 22 Issue: 1, 67 - 76, 15.04.2021

Gülşah Mersin Ünsal Açıkel

https://doi.org/10.23902/trkjnat.817451

Cited By: 2

Abstract

Yeasts can accumulate heavy metals and grow in acidic media. In the present study, it was shown that Candida yeasts in an aqueous solution accumulate single Cu(II) and Ni(II) cations. The effect of heavy metal ions on the specific growth rate of biomasses and the uptake of metal ions during the growth phase was investigated in a batch system. Bioaccumulation efficiency decreased with increasing metal ion concentrations at constant sucrose concentrations. Both the specific growth rate and the biomass concentration were more inhibited in the bioaccumulation media containing Ni(II) ions singly as compared with the bioaccumulation media containing Cu(II) ions singly. The maximum specific growth rate and the saturation constant of yeasts were examined with a double-reciprocal form of Monod equation. Metal uptake performance decreased from 81.68% to 46.28% with increasing Ni(II) concentration from 25 mg/L to 250 mg/L for Candida lipolytica. Candida biomasses may be an alternative way of removal of heavy metals from wastewaters and may constitute a sample to produce new biomass. The study showed that Candida yeasts can be used as economical biomass due to their metal resistance and efficient production.

Keywords

Bioaccumulation, Heavy metal cations, wastewater, Candida, biomass

Supporting Institution

Cumhuriyet Üniversitesi

Project Number

Bilimsel Araştırma Projeleri M-354

Thanks

The financial support provided by the Cumhuriyet University Scientific Research Projects Unit (BAP- M-354 coded project) is gratefully acknowledged.

References

1. Açikel, U. & Alp, T. 2009. A study on the inhibition kinetics of bioaccumulation of Cu(II) and Ni(II) ions using Rhizopus delemar. Journal of Hazardous Materials, 168(2-3): 1449-1458. https://doi.org/10.1016/j.jhazmat.2009.03.040
2. Aksu, Z. & Dönmez, G. 2000. The use of molasses in copper (II) containing wastewaters: effects on growth and copper (II) bioaccumulation properties of Kluyveromyces marxianus. Process Biochemistry, 36(5): 451-458. https://doi.org/10.1016/S0032-9592(00)00234-X
3. Baysal, Z., Çinar, E., Bulut, Y., Alkan, H. & Dogru, M. 2009. Equilibrium and thermodynamic studies on biosorption of Pb(II) onto Candida albicans biomass. Journal of Hazardous Materials, 161(1): 62-67. https://doi.org/10.1016/j.jhazmat.2008.02.122
4. Brady, D. & Duncan, J.R. 1994. Bioaccumulation of metal cations by Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 41: 149-154. https://doi.org/10.1007/BF00166098
5. Chen, S., Yin, H., Ye, J., Peng, H., Liu, Z., Dand, Z. & Chang, J. 2014. Influence of co-existed benzo[a]pyrene and copper on the cellular characteristics of Stenotrophomonas maltophilia during biodegradation and transformation. Bioresource Technology, 158: 181-187. https://doi.org/10.1016/j.biortech.2014.02.020
6. Cottet, C., Ramirez-Tapias, Y.A., Delgado, J.F., de la Osa, O., Salvay, A.G. & Peltzer, M.A. 2020. Biobased Materials from Microbial Biomass and Its Derivatives. Materials, 13(6): 1263. https://doi.org/10.3390/ma13061263
7. Dupont, C.L., Grass, G. & Rensing, C. 2011. Copper toxicity and the origin of bacterial resistance-New insights and applications. Metallomics, (3): 1109-1118. https://doi.org/10.1039/c1mt00107h
8. Evirgen, O.A. & Sag Acikel, Y. 2014. Simultaneous copper bioaccumulation, growth and lipase production of Rhizopus delemar in molasses medium: optimisation of environmental conditions using RSM. Chemistry and Ecology, 30(1): 39-51. https://doi.org/10.1080/02757540.2013.827670
9. Fadel M., Hassanein, N.M., Elshafei, M.M., Mostafa, A.H., Ahmed, M.A. & Khater, H.M. 2017. Biosorption of manganese from groundwater by biomass of Saccharomyces cerevisiae. HBRC Journal. 13(1): 106-113. https://doi.org/10.1016/j.hbrcj.2014.12.006
10. Fashola, M.O., Ngole-Jeme, V.M. & Babalola, O.O. 2016. Heavy metal pollution from gold mines: Environmental effects and bacterial strategies for resistance. International Journal of Environmental Research and Public Health, (13): 1047. https://doi.org/10.3390/ijerph13111047
11. Giner-Lamia, J., L´opez-Maury, L., Florencio, F.J. & Janssen, P.J. 2014. Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803. PLOS ONE, 9 (9): e108912. https://doi.org/10.1371/journal.pone.0108912
12. Gönen, F. & Aksu, Z. 2008. Use of response surface methodology (RSM) in the evaluation of growth and copper(II) bioaccumulation properties of Candida utilis in molasses medium. Journal of Hazardous Materials, 154(1-3): 731-738. https://doi.org/10.1016/j.jhazmat.2007.10.086
13. Gourdon, R., Bhende, S., Rus, E. & Sofer, S.S. 1990. Comparison of cadmium biosorption by Gram-positive and Gram-negative bacteria from activated sludge. Biotechnology Letters, 12: 839-842. https://doi.org/10.1007/BF01022606
14. Honfi, K., Tálos, K., Kőnig-Péter, A., Kilár, F. & Pernyeszi, T. 2016. Copper(II) and Phenol Adsorption by Cell Surface Treated Candida tropicalis Cells in Aqueous Suspension. Water, Air & Soil Pollution, 227: 1-14. https://doi.org/10.1007/s11270-016-2751-0
15. Javanbakht, V., Alavi, S.A. & Zilouei, H. 2013. Mechanisms of heavy metal removal using microorganisms as biosorbent. Water Science and Technology, 69(9): 1775-1787. https://doi.org/10.2166/wst.2013.718
16. Legorreta-Castañeda, A.J., Lucho-Constantino, C.A., Beltrán-Hernández, R.I., Coronel-Olivares, C. & Vázquez-Rodríguez, G.A. 2020. Biosorption of Water Pollutants by Fungal Pellets. Water, 12(4): 1155-1193. https://doi.org/10.3390/w12041155
17. Liu, Y. 2007. Overview of some theoretical approaches for derivation of the Monod equation. Applied Microbiology and Biotechnology, 73: 1241-1250 https://doi.org/10.1007/s00253-006-0717-7
18. Luk, C.H.J., Yip, J., Yuen, C.W.M., Pang, S.W., Lam, K.H. & Kan, C.W. 2017. Biosorption Performance of Encapsulated Candida krusei for the removal of Copper(II). Scientific Reports, 7: 1-9. https://doi.org/10.1038/s41598-017-02350-7
19. Luna, J.M., Rufino, R.D. & Sarubbo, L.A. 2016. Biosurfactant from Candida sphaerica UCP0995 exhibiting heavy metal remediation properties. Process Safety and Environmental Protection, 102: 558-566. https://doi.org/10.1016/j.psep.2016.05.010
20. Malik, A. 2004. Metal bioremediation through growing cells. Environment International, 30(2): 261-78. https://doi.org/10.1016/j.envint.2003.08.001
21. Modak, J.M. & Natarajan, K.A. 1995. Biosorption of metals using nonliving biomass-A review. Mining, Metallurgy & Exploration, 12: 189-196. https://doi.org/10.1007/BF03403102
22. Monod, J. 1949. The growth of bacterial cultures. Annual Reviews in Microbiology, 3(1): 371-394. https://mcb.berkeley.edu/labs/garcia/sites/mcb.berkeley.edu.labs.garcia/files/Teaching/2017-MCB137/Monod1949.pdf (Date accessed: 22.11.2020)
23. Pawan, K.R. & Devi, R. 2018. Heavy metal tolerance and adaptability assessment of indigenous filamentous fungi isolated from industrial wastewater and sludge samples. Beni-Suef University Journal of Basic and Applied Sciences, 7(4): 688-694. https://doi.org/10.1016/j.bjbas.2018.08.001
24. Podder, M.S. & Majumder, C.B. 2019. Bacteria immobilization on neem leaves/MnFe2O4 composite surface for removal of As(III) and As(V) from wastewater. Arabian Journal of Chemistry, 12: 3263-3288. https://doi.org/10.1016/j.arabjc.2015.08.025
25. Raspor P. & Zupan J. 2006. Yeasts in Extreme Environments. pp. 371-372. In: Péter G.& Rosa C.A. (eds). Biodiversity and Ecophysiology of Yeasts. Springer-Verlang, Berlin, 580 pp. https://doi.org/10.1007/3-540-30985-3_15
26. Razack, S.A., Velayutham, V. & Thangavelu, V. 2013. Medium optimization for the production of exopolysaccharide by Bacillus subtilis using synthetic sources and agro wastes. Turkish Journal of Biology, 37: 280-288. https://doi.org/10.3906/biy-1206-50
27. Redha, A.A. 2020. Removal of heavy metals from aqueous media by biosorption. Arab Journal of Basic and Applied Sciences, 27(1): 183-193. https://doi.org/10.1080/25765299.2020.1756177
28. Rehman, A. & Anjum, M.S. 2011. Multiple metal tolerance and biosorption of cadmium by Candida tropicalis isolated from industrial effluents: glutathione as detoxifying agent. Environmental Monitoring and Assessment, 174: 585-595. https://doi.org/10.1007/s10661-010-1480-x
29. Rehman, A. & Anjum, M.S. 2010. Cadmium Uptake by Yeast, Candida tropicalis, Isolated from Industrial Effluents and Its Potential Use in Wastewater Clean-Up Operations. Water, Air and Soil Pollution, 205: 149-159. https://doi.org/10.1007/s11270-009-0062-4
30. Şengör, S.S., Barua, S., Gikas, P., Ginn, T.R., Peyton, B., Sani, R.K., & Spycher, N.F. 2009. Influence Of Heavy Metals On Microbial Growth Kinetics Including Lag Time: Mathematical Modeling And Experimental Verification. Environmental Toxicology and Chemistry, 28: 2020-2029. https://doi.org/10.1897/08-273.1
31. Sandell, E.B. 1950. Colorimetric Determination of Traces of Metals Volume III. pp. 304-475. In: Clarke, B.L. & Kolthoff, I.M. (eds). Chemical Analysis A Series of Monographs on Analytical Chemistry And Its Applications. Interscience Publishers INC, London, 688 pp.
32. Tchounwou, P.B., Yedjou, C.G., Patlolla, A.K. & Sutton, D. J. 2012. Heavy metal toxicity and the environment. Experientia Supplementum, 101: 133-164. https://doi.org/10.1007/978-3-7643-8340-4_6
33. Tripathi, A, & Ranjan, M.R. 2015. Heavy Metal Removal from Wastewater Using Low Cost Adsorbents. Journal of Bioremediation & Biodegration, 6: 315-320. http://dx.doi.org/10.4172/2155-6199.1000315
34. Volland, S., Bayer, E., Baumgartner, V., Andosch, A., Lütz, C., Sima, E. & Lütz-Meindl, U. 2014. Rescue of heavy metal effects on cell physiology of the algal model system Micrasterias by divalent ions. Journal of Plant Physiology, 171(2): 154-163. https://doi.org/10.1016/j.jplph.2013.10.002
35. Waldron, K.J. & Robinson, N.J. 2009. How do bacterial cells ensure that metalloproteins get the correct metal? Nature Reviews Microbiology, 7: 25-35. https://doi.org/10.1038/nrmicro2057
36. Wołowiec, M., Komorowska-Kaufman, M., Pruss, A., Rzepa, G. & Bajda, T. 2019. Removal of Heavy Metals and Metalloids from Water Using Drinking Water Treatment Residuals as Adsorbents: A Review. Minerals, 9(8): 487-504. https://doi.org/10.3390/min9080487
37. Zha, F., Wang, H., Xu, L., Yang, C., Kang, B., Chu, C., Deng, Y. & Tan, X. 2020. Initial feasibility study in adsorption capacity and mechanism of soda residue on lead (II)-contaminated soil in solidification/stabilization technology. Environmental Earth Sciences, 79: 1-12. https://doi.org/10.1007/s12665-020-08990-9

Year 2021, Volume: 22 Issue: 1, 67 - 76, 15.04.2021

Gülşah Mersin Ünsal Açıkel

https://doi.org/10.23902/trkjnat.817451

Cited By: 2

Abstract

Mayalar, asidik ortamda büyüyebilir ve ağır metalleri biriktirebilir. Bu çalışma, Candida türü mayaların sulu çözeltilerden tekli Cu(II) ve Ni(II) katyonlarını biriktirdiğini göstermiştir. Ağır metal iyonlarının, biyokütlelerin spesifik büyüme hızı ve büyüme periyodu boyunca metal iyonlarını giderimi üzerindeki etkisi, bir kesikli sistemde araştırılmıştır. Sabit sakaroz derişiminde, metal iyonu derişimi arttıkça, biyobirikim verimi azalmıştır. Hem spesifik büyüme hızı hem de biyokütle konsantrasyonu, tek başına Cu(II) iyonları içeren biyoakümülasyon ortamına kıyasla Ni(II) iyonları içeren biyoakümülasyon ortamında daha fazla inhibe edilmiştir. Mayaların maksimum özgül büyüme hızı ve doygunluk sabiti, Monod denkleminin çift-karşılıklı formu ile incelenmiştir. Candida lipolytica’nın metal giderim performansı Ni(II) derişiminin 25 mg/L’den 250 mg/L'ye çıkmasıyla % 81,68'den % 46,28'e düşmüştür. Candida biyokütleleri, ağır metallerin atık sulardan gideriminde alternatif bir yol olabilir ve yeni biyokütle üretimi için bir örnek oluşturabilir. Bu çalışma, Candida mayalarının, metal direnci ve verimli üretimleri nedeniyle ekonomik biyokütle olarak kullanılabileceğini göstermektedir.

Project Number

Bilimsel Araştırma Projeleri M-354

References

1. Açikel, U. & Alp, T. 2009. A study on the inhibition kinetics of bioaccumulation of Cu(II) and Ni(II) ions using Rhizopus delemar. Journal of Hazardous Materials, 168(2-3): 1449-1458. https://doi.org/10.1016/j.jhazmat.2009.03.040
2. Aksu, Z. & Dönmez, G. 2000. The use of molasses in copper (II) containing wastewaters: effects on growth and copper (II) bioaccumulation properties of Kluyveromyces marxianus. Process Biochemistry, 36(5): 451-458. https://doi.org/10.1016/S0032-9592(00)00234-X
3. Baysal, Z., Çinar, E., Bulut, Y., Alkan, H. & Dogru, M. 2009. Equilibrium and thermodynamic studies on biosorption of Pb(II) onto Candida albicans biomass. Journal of Hazardous Materials, 161(1): 62-67. https://doi.org/10.1016/j.jhazmat.2008.02.122
4. Brady, D. & Duncan, J.R. 1994. Bioaccumulation of metal cations by Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 41: 149-154. https://doi.org/10.1007/BF00166098
5. Chen, S., Yin, H., Ye, J., Peng, H., Liu, Z., Dand, Z. & Chang, J. 2014. Influence of co-existed benzo[a]pyrene and copper on the cellular characteristics of Stenotrophomonas maltophilia during biodegradation and transformation. Bioresource Technology, 158: 181-187. https://doi.org/10.1016/j.biortech.2014.02.020
6. Cottet, C., Ramirez-Tapias, Y.A., Delgado, J.F., de la Osa, O., Salvay, A.G. & Peltzer, M.A. 2020. Biobased Materials from Microbial Biomass and Its Derivatives. Materials, 13(6): 1263. https://doi.org/10.3390/ma13061263
7. Dupont, C.L., Grass, G. & Rensing, C. 2011. Copper toxicity and the origin of bacterial resistance-New insights and applications. Metallomics, (3): 1109-1118. https://doi.org/10.1039/c1mt00107h
8. Evirgen, O.A. & Sag Acikel, Y. 2014. Simultaneous copper bioaccumulation, growth and lipase production of Rhizopus delemar in molasses medium: optimisation of environmental conditions using RSM. Chemistry and Ecology, 30(1): 39-51. https://doi.org/10.1080/02757540.2013.827670
9. Fadel M., Hassanein, N.M., Elshafei, M.M., Mostafa, A.H., Ahmed, M.A. & Khater, H.M. 2017. Biosorption of manganese from groundwater by biomass of Saccharomyces cerevisiae. HBRC Journal. 13(1): 106-113. https://doi.org/10.1016/j.hbrcj.2014.12.006
10. Fashola, M.O., Ngole-Jeme, V.M. & Babalola, O.O. 2016. Heavy metal pollution from gold mines: Environmental effects and bacterial strategies for resistance. International Journal of Environmental Research and Public Health, (13): 1047. https://doi.org/10.3390/ijerph13111047
11. Giner-Lamia, J., L´opez-Maury, L., Florencio, F.J. & Janssen, P.J. 2014. Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803. PLOS ONE, 9 (9): e108912. https://doi.org/10.1371/journal.pone.0108912
12. Gönen, F. & Aksu, Z. 2008. Use of response surface methodology (RSM) in the evaluation of growth and copper(II) bioaccumulation properties of Candida utilis in molasses medium. Journal of Hazardous Materials, 154(1-3): 731-738. https://doi.org/10.1016/j.jhazmat.2007.10.086
13. Gourdon, R., Bhende, S., Rus, E. & Sofer, S.S. 1990. Comparison of cadmium biosorption by Gram-positive and Gram-negative bacteria from activated sludge. Biotechnology Letters, 12: 839-842. https://doi.org/10.1007/BF01022606
14. Honfi, K., Tálos, K., Kőnig-Péter, A., Kilár, F. & Pernyeszi, T. 2016. Copper(II) and Phenol Adsorption by Cell Surface Treated Candida tropicalis Cells in Aqueous Suspension. Water, Air & Soil Pollution, 227: 1-14. https://doi.org/10.1007/s11270-016-2751-0
15. Javanbakht, V., Alavi, S.A. & Zilouei, H. 2013. Mechanisms of heavy metal removal using microorganisms as biosorbent. Water Science and Technology, 69(9): 1775-1787. https://doi.org/10.2166/wst.2013.718
16. Legorreta-Castañeda, A.J., Lucho-Constantino, C.A., Beltrán-Hernández, R.I., Coronel-Olivares, C. & Vázquez-Rodríguez, G.A. 2020. Biosorption of Water Pollutants by Fungal Pellets. Water, 12(4): 1155-1193. https://doi.org/10.3390/w12041155
17. Liu, Y. 2007. Overview of some theoretical approaches for derivation of the Monod equation. Applied Microbiology and Biotechnology, 73: 1241-1250 https://doi.org/10.1007/s00253-006-0717-7
18. Luk, C.H.J., Yip, J., Yuen, C.W.M., Pang, S.W., Lam, K.H. & Kan, C.W. 2017. Biosorption Performance of Encapsulated Candida krusei for the removal of Copper(II). Scientific Reports, 7: 1-9. https://doi.org/10.1038/s41598-017-02350-7
19. Luna, J.M., Rufino, R.D. & Sarubbo, L.A. 2016. Biosurfactant from Candida sphaerica UCP0995 exhibiting heavy metal remediation properties. Process Safety and Environmental Protection, 102: 558-566. https://doi.org/10.1016/j.psep.2016.05.010
20. Malik, A. 2004. Metal bioremediation through growing cells. Environment International, 30(2): 261-78. https://doi.org/10.1016/j.envint.2003.08.001
21. Modak, J.M. & Natarajan, K.A. 1995. Biosorption of metals using nonliving biomass-A review. Mining, Metallurgy & Exploration, 12: 189-196. https://doi.org/10.1007/BF03403102
22. Monod, J. 1949. The growth of bacterial cultures. Annual Reviews in Microbiology, 3(1): 371-394. https://mcb.berkeley.edu/labs/garcia/sites/mcb.berkeley.edu.labs.garcia/files/Teaching/2017-MCB137/Monod1949.pdf (Date accessed: 22.11.2020)
23. Pawan, K.R. & Devi, R. 2018. Heavy metal tolerance and adaptability assessment of indigenous filamentous fungi isolated from industrial wastewater and sludge samples. Beni-Suef University Journal of Basic and Applied Sciences, 7(4): 688-694. https://doi.org/10.1016/j.bjbas.2018.08.001
24. Podder, M.S. & Majumder, C.B. 2019. Bacteria immobilization on neem leaves/MnFe2O4 composite surface for removal of As(III) and As(V) from wastewater. Arabian Journal of Chemistry, 12: 3263-3288. https://doi.org/10.1016/j.arabjc.2015.08.025
25. Raspor P. & Zupan J. 2006. Yeasts in Extreme Environments. pp. 371-372. In: Péter G.& Rosa C.A. (eds). Biodiversity and Ecophysiology of Yeasts. Springer-Verlang, Berlin, 580 pp. https://doi.org/10.1007/3-540-30985-3_15
26. Razack, S.A., Velayutham, V. & Thangavelu, V. 2013. Medium optimization for the production of exopolysaccharide by Bacillus subtilis using synthetic sources and agro wastes. Turkish Journal of Biology, 37: 280-288. https://doi.org/10.3906/biy-1206-50
27. Redha, A.A. 2020. Removal of heavy metals from aqueous media by biosorption. Arab Journal of Basic and Applied Sciences, 27(1): 183-193. https://doi.org/10.1080/25765299.2020.1756177
28. Rehman, A. & Anjum, M.S. 2011. Multiple metal tolerance and biosorption of cadmium by Candida tropicalis isolated from industrial effluents: glutathione as detoxifying agent. Environmental Monitoring and Assessment, 174: 585-595. https://doi.org/10.1007/s10661-010-1480-x
29. Rehman, A. & Anjum, M.S. 2010. Cadmium Uptake by Yeast, Candida tropicalis, Isolated from Industrial Effluents and Its Potential Use in Wastewater Clean-Up Operations. Water, Air and Soil Pollution, 205: 149-159. https://doi.org/10.1007/s11270-009-0062-4
30. Şengör, S.S., Barua, S., Gikas, P., Ginn, T.R., Peyton, B., Sani, R.K., & Spycher, N.F. 2009. Influence Of Heavy Metals On Microbial Growth Kinetics Including Lag Time: Mathematical Modeling And Experimental Verification. Environmental Toxicology and Chemistry, 28: 2020-2029. https://doi.org/10.1897/08-273.1
31. Sandell, E.B. 1950. Colorimetric Determination of Traces of Metals Volume III. pp. 304-475. In: Clarke, B.L. & Kolthoff, I.M. (eds). Chemical Analysis A Series of Monographs on Analytical Chemistry And Its Applications. Interscience Publishers INC, London, 688 pp.
32. Tchounwou, P.B., Yedjou, C.G., Patlolla, A.K. & Sutton, D. J. 2012. Heavy metal toxicity and the environment. Experientia Supplementum, 101: 133-164. https://doi.org/10.1007/978-3-7643-8340-4_6
33. Tripathi, A, & Ranjan, M.R. 2015. Heavy Metal Removal from Wastewater Using Low Cost Adsorbents. Journal of Bioremediation & Biodegration, 6: 315-320. http://dx.doi.org/10.4172/2155-6199.1000315
34. Volland, S., Bayer, E., Baumgartner, V., Andosch, A., Lütz, C., Sima, E. & Lütz-Meindl, U. 2014. Rescue of heavy metal effects on cell physiology of the algal model system Micrasterias by divalent ions. Journal of Plant Physiology, 171(2): 154-163. https://doi.org/10.1016/j.jplph.2013.10.002
35. Waldron, K.J. & Robinson, N.J. 2009. How do bacterial cells ensure that metalloproteins get the correct metal? Nature Reviews Microbiology, 7: 25-35. https://doi.org/10.1038/nrmicro2057
36. Wołowiec, M., Komorowska-Kaufman, M., Pruss, A., Rzepa, G. & Bajda, T. 2019. Removal of Heavy Metals and Metalloids from Water Using Drinking Water Treatment Residuals as Adsorbents: A Review. Minerals, 9(8): 487-504. https://doi.org/10.3390/min9080487
37. Zha, F., Wang, H., Xu, L., Yang, C., Kang, B., Chu, C., Deng, Y. & Tan, X. 2020. Initial feasibility study in adsorption capacity and mechanism of soda residue on lead (II)-contaminated soil in solidification/stabilization technology. Environmental Earth Sciences, 79: 1-12. https://doi.org/10.1007/s12665-020-08990-9

There are 37 citations in total.

Details

Primary Language	English
Subjects	Environmental Sciences
Journal Section	Research Article/Araştırma Makalesi
Authors	Gülşah Mersin This is me 0000-0002-2852-6114 Ünsal Açıkel 0000-0003-4969-8502
Project Number	Bilimsel Araştırma Projeleri M-354
Publication Date	April 15, 2021
Submission Date	October 28, 2020
Acceptance Date	April 6, 2021
Published in Issue	Year 2021 Volume: 22 Issue: 1

Cite

APA	Mersin, G., & Açıkel, Ü. (2021). PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS. Trakya University Journal of Natural Sciences, 22(1), 67-76. https://doi.org/10.23902/trkjnat.817451
AMA	Mersin G, Açıkel Ü. PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS. Trakya Univ J Nat Sci. April 2021;22(1):67-76. doi:10.23902/trkjnat.817451
Chicago	Mersin, Gülşah, and Ünsal Açıkel. “PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS”. Trakya University Journal of Natural Sciences 22, no. 1 (April 2021): 67-76. https://doi.org/10.23902/trkjnat.817451.
EndNote	Mersin G, Açıkel Ü (April 1, 2021) PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS. Trakya University Journal of Natural Sciences 22 1 67–76.
IEEE	G. Mersin and Ü. Açıkel, “PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS”, Trakya Univ J Nat Sci, vol. 22, no. 1, pp. 67–76, 2021, doi: 10.23902/trkjnat.817451.
ISNAD	Mersin, Gülşah - Açıkel, Ünsal. “PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS”. Trakya University Journal of Natural Sciences 22/1 (April 2021), 67-76. https://doi.org/10.23902/trkjnat.817451.
JAMA	Mersin G, Açıkel Ü. PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS. Trakya Univ J Nat Sci. 2021;22:67–76.
MLA	Mersin, Gülşah and Ünsal Açıkel. “PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS”. Trakya University Journal of Natural Sciences, vol. 22, no. 1, 2021, pp. 67-76, doi:10.23902/trkjnat.817451.
Vancouver	Mersin G, Açıkel Ü. PRODUCTION OF Candida BIOMASSES FOR HEAVY METAL REMOVAL FROM WASTEWATERS. Trakya Univ J Nat Sci. 2021;22(1):67-76.

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The Effects of Heavy Metals and Molasses on Enzyme Activity of Candida Yeast

Cumhuriyet Science Journal

https://doi.org/10.17776/csj.1127921

Yeast-driven valorization of agro-industrial wastewater: an overview

Environmental Monitoring and Assessment

https://doi.org/10.1007/s10661-023-11863-w

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