Research Article
BibTex RIS Cite

Determination of Serum Lipid Profile and Lipoprotein Levels of Sheep with Naturally Acute Babesiosis

Year 2017, Volume: 28 Issue: 1, 1 - 4, 20.04.2017

Abstract

In this study, which was done to determine the
lipid profile and the variations about the levels of lipoprotein in the blood
of sheep naturally infected with Babesia
ovis
, 3-5 year old, 30 infected and 15 healthy, totally 45 Morkaraman breed
sheep raised in Erciş county of Van city were used as materials. The
measurement of triglyceride, total cholesterol, LDL and HDL were studied by
using enzymatic colorimetric method. VLDL levels were calculated by formula.
The percentages of free fatty acids, triglyceride, phospholipids, cholesterol
and cholesteryl esters were measured by using thin layer chromatography. In the
infected group it was determined that the levels of triglyceride (P<0.001)
and VLDL (P<0.001) statistically increased, but the HDL (P<0.05) decreased
compared to control group. After the chromatographic determination of lipid
amounts (%), in the infected group it was determined that triglyceride amount
(P<0.001) statistically increased, but phospholipids (p<0.05) and
cholesterol esters amounts (P<0.01) decreased compared to control group. As
a result, it is said to be important that lipid levels in sheep 
babesiosis and lipoprotein levels are important for
animals in terms of prognosis.

References

  • Adamu S, Ige AA, Jatau ID, Neils JS, Nicodemus M, Useh NM, Bisalla M, Ibrahim NDG, Nok AJ, Esievo KAN (2008). Changes in the serum profiles of lipids and cholesterol in sheep experimental model of acute African trypanosomosis. Afr J Biotechnol, 7, 2090-2098. Aksakal M, Özer E (1987). Investigations on hematological values and vitamin E levels of sera before and after treatment with antihelmentic drugs in Akkaraman sheep. AÜ Vet Fak Derg, 34, 72-84. Asadian A, Mirhadi SA, Mezes M (1995). Seasonal variation in the concentration of vitamins A and E in the blood plasma of fat-tailed sheep. Acta Vet Hung, 43 (4), 453-461. Astrup HN, Nedkvitne JJ (1987). Magnesium, glucose and cholesterol in serum of pregnant ewes fed silage and bam dried hay. Norw J Agric Sci, 1, 75-80. Champe PC, Harvey RA (1997). Biyokimya, Lippincott’s Illustrated Review Serisinden, Nobel Tıp Kitabevleri Ltd Şti, İstanbul s, 180-206-207-216-221. Dinçer Ş, Sayın F, Karaer Z, Çakmak A, Friedhoff KT, Muller I, İnci A, Yukarı BA, Eren H (1991). Karadeniz bölgesi sığırlarında bulunan kan parazitlerinin sero-insidensi üzerine araştırmalar. Ankara Üniv Vet Fak Derg, 38, 206-226. Durrington P, Sniderman A (2001). Hiperlipidemi, Fast Facts, Health Pres. Ginsburg H, Atamina H (1994). The redox status of malaria infected erythrocytes, an overview with an emphasis on unresolved problems. Parasite, 18, 5-13. Goodger BV, Wright IG, Mahoney DF (1981). Babesia bovis (Argentina), studies of plasma lipids and lipoproteins during acute infections in cattle. Z Parasitenkd, 65(3), 271-276. Gökçe E, Kırmızıgül AH, Taşçı GT, Uzlu E, Gündüz N, Vatansever Z (2013). Türkiye’de köpeklerde Babesia canis’in klinik ve parazitolojik olarak ilk tespiti. Kafkas Univ Vet Fak Derg, 19 (4), 717-720. Gündüz H, Mert N (1997). Farklı ırklardaki ithal etçi koyunlarda serum lipoprotein düzeyleri. YYÜ Vet Fak Derg, 8, 25-27. Hallford DM, Galyean ML (1982). Serum profiles in fine-wool sheep. Bovine Pr, 3(4), 26-32. Hoffmann G, Horchner F, Schein E, Gerber H (1971). Saisoneles auftreten von zecken und Piroplasmen bei haustieren in den Siatischen Provinzen der Türkei. Ber, Münch, Tierärztl, Wschr, 94 (8), 152-156. İnci A (1992). Ankara'nın Çubuk ilçesinde sığırlarda babesiosis'in seroinsidensi üzerine araştırmalar. Ankara Üniv Vet Fak Derg, 39, 153-167. Kaneko JJ, Harvey JW, Bruss ML (2008). Clinical Biochemistry of Domestic Animals. 6th Ed. Academic Press, Amsterdam. Kontaş T, Salmanoğlu B, Çakmak A, Beşkaya A (2008). Relative changes in serum lipid-lipoprotein and trace element levels in cattle babesiosis. Medycyna Wet, 64, 1104-1106. Kurt D, Yokuş B, Çakır DÜ, Denli O (2008). Investigation levels of certain serum biochemistry components and minerals of pasturing Akkaraman Sheeps in Adiyaman province. Dicle Univ Vet Fak Derg, 1, 34-37. Kutsal A, Alpan O, Arpacık R (1990). İstatistik Uygulamalar. Dizgi-Baskı Basımevi. Ankara. Lacetera N, Bernabucci U, Ronchi B, Nardone A (1999). The effects of injectable sodium selenite on immune function and milk production in Sardinian sheep receiving adequate dietary selenium. Vet Res, 30, 363-370. Man EB, Kartin BL, Durlacher SH, Peters JP (1945). The lipids of serum and liver in patients with hepatic diseases. J Clin Invest, 24 (5), 623-643. Mimioğlu MM, Ulutaş M, Güler S (1973). Yurdumuz Sığırlarında Theileriosis Etkenleri ve Diğer Kan Parazitleri. Ajans-Türk Matbaacılık Sanayii, Ankara. Nizamlıoglu M, Tiftik A M, Turgut Tras B (1991). Kuzuların Beyaz kas hastalığında vitamin E, glutamik okzalasetik transaminaz (GOT), kreatinkinaz (CK) ve Laktat Dehidrogenaz (LDH) aktivitelerinin araştırılması. Doga Tr J Vet and Anim Sci, 15, 59-64. Özer E, Yılmaz K, Erkal N, Şaki E C, Turan T, Angın M, Öztürk G (1995). Bazı Eimeria türleri ile deneysel olarak enfekte edilen erkek akkaraman kuzularda demir ve bakır bağlama kapasitesi. FÜ Sağ Bil Derg, 9 (2), 245-257. Özpınar H, Schweigert FJ, Özpınar A, Wierich M, Senel HS (1988). Aenderung der verteilung der fettlöslichen vitamine auf die lipoproteinfraktionen bei saugkaelbern und kühen in abhaengigkeit von der geburt. Berl, Münch, Tieraerztl, Wschr, 101, 383-387. Philips GB (1960). The lipid composition of serum in patients with liver disease. J Clin Invest, 39, 1639-1650. Rommel M (2000). Protozooenenfektionen der Wiederkaeuer. Eds. M, Rommel J, Eckert E, Kutzer W, Körting T, Schnieder. Veterinarmedizinische Parasitologie. 5, Vollstaending neubearbeitete Auflage. Berlin, Blackwell Wissenschafst-Verlag, p, 121-191. Russel L, Mc Dowell J (1989). Vitamins in Animal Nutrition, Academic Press Inc, San Diego, California. Sato T, Liang K, Vaziri ND (2002). Downregulation of lipoprotein lipase and VLDL receptor in rats with focal glomerulosclerosis. Kidney Int, 61, 157. Singh PP, Barjatiya MK, Dhing S, Bhatnagar R, Kothari S, Dhar V (2001). Evidence suggesting that high intake of fluoride provokes nephrolithiasis in tribal populations. Urol Res, 29 (4), 238- 44. Tanyüksel M, Sayal A, Aydın A (1995). Paraziter hastalıklarda eser elementlerin düzeyleri. Türk Parazitol Derg, 19 (2), 315-321. Valentin A, Rigomier D, Précigout E, Carcy B, Gorenflot A, Schrével (1991). Lipid trafficking between high density lipoproteins and Babesia divergens-infected human erythrocytes. J Biol Cell, 73(1), 63-70.

Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili ve Lipoprotein Düzeylerinin İncelenmesi

Year 2017, Volume: 28 Issue: 1, 1 - 4, 20.04.2017

Abstract

Babesia
ovis
ile doğal enfekte olan koyunlarda kandaki lipit profilini ve
lipoprotein düzeylerindeki değişimi saptamak için yapılan bu çalışmada canlı
materyal olarak Van ili Erciş ilçesinde yetiştirilen 3-5 yaşları arasındaki,
toplam 45 Morkaraman ırkı koyun kullanıldı (30 enfekte ve 15 sağlıklı koyun).
Serum örneklerinde trigliserit, total kolesterol, LDL ve HDL ölçümü enzimatik
kolorimetrik yöntem kullanılarak çalışıldı. VLDL düzeyleri formülle hesaplandı.
Serbest yağ asitleri, trigliserit, fosfolipit, kolesterol ve kolesterol esterlerinin
% miktarları ise ince tabaka kromatografisi kullanılarak ölçüldü. Enfekte
hayvanlarda sağlıklı kontrollere göre trigliserit (P<0.001) ve VLDL
düzeylerinin (P<0.001) istatistik olarak önemle arttığı, HDL düzeyinin
(P<0.05) ise azaldığı tespit edildi. Lipit miktarlarının (%) kromatografik
olarak incelenmesi sonucunda enfekte hayvanlarda sağlıklı kontrollere göre
trigliserit miktarının (P<0.001) istatistik olarak önemle arttığı,
fosfolipit (P<0.05) ve kolesterol esterlerinin miktarının (P<0.01) ise
azaldığı saptandı. Sonuç olarak, koyun babesiosisinde lipit profilinin ve
lipoprotein düzeylerinin bilinmesinin hayvanlarda prognoz açısından önemli
olduğu söylenebilir.

References

  • Adamu S, Ige AA, Jatau ID, Neils JS, Nicodemus M, Useh NM, Bisalla M, Ibrahim NDG, Nok AJ, Esievo KAN (2008). Changes in the serum profiles of lipids and cholesterol in sheep experimental model of acute African trypanosomosis. Afr J Biotechnol, 7, 2090-2098. Aksakal M, Özer E (1987). Investigations on hematological values and vitamin E levels of sera before and after treatment with antihelmentic drugs in Akkaraman sheep. AÜ Vet Fak Derg, 34, 72-84. Asadian A, Mirhadi SA, Mezes M (1995). Seasonal variation in the concentration of vitamins A and E in the blood plasma of fat-tailed sheep. Acta Vet Hung, 43 (4), 453-461. Astrup HN, Nedkvitne JJ (1987). Magnesium, glucose and cholesterol in serum of pregnant ewes fed silage and bam dried hay. Norw J Agric Sci, 1, 75-80. Champe PC, Harvey RA (1997). Biyokimya, Lippincott’s Illustrated Review Serisinden, Nobel Tıp Kitabevleri Ltd Şti, İstanbul s, 180-206-207-216-221. Dinçer Ş, Sayın F, Karaer Z, Çakmak A, Friedhoff KT, Muller I, İnci A, Yukarı BA, Eren H (1991). Karadeniz bölgesi sığırlarında bulunan kan parazitlerinin sero-insidensi üzerine araştırmalar. Ankara Üniv Vet Fak Derg, 38, 206-226. Durrington P, Sniderman A (2001). Hiperlipidemi, Fast Facts, Health Pres. Ginsburg H, Atamina H (1994). The redox status of malaria infected erythrocytes, an overview with an emphasis on unresolved problems. Parasite, 18, 5-13. Goodger BV, Wright IG, Mahoney DF (1981). Babesia bovis (Argentina), studies of plasma lipids and lipoproteins during acute infections in cattle. Z Parasitenkd, 65(3), 271-276. Gökçe E, Kırmızıgül AH, Taşçı GT, Uzlu E, Gündüz N, Vatansever Z (2013). Türkiye’de köpeklerde Babesia canis’in klinik ve parazitolojik olarak ilk tespiti. Kafkas Univ Vet Fak Derg, 19 (4), 717-720. Gündüz H, Mert N (1997). Farklı ırklardaki ithal etçi koyunlarda serum lipoprotein düzeyleri. YYÜ Vet Fak Derg, 8, 25-27. Hallford DM, Galyean ML (1982). Serum profiles in fine-wool sheep. Bovine Pr, 3(4), 26-32. Hoffmann G, Horchner F, Schein E, Gerber H (1971). Saisoneles auftreten von zecken und Piroplasmen bei haustieren in den Siatischen Provinzen der Türkei. Ber, Münch, Tierärztl, Wschr, 94 (8), 152-156. İnci A (1992). Ankara'nın Çubuk ilçesinde sığırlarda babesiosis'in seroinsidensi üzerine araştırmalar. Ankara Üniv Vet Fak Derg, 39, 153-167. Kaneko JJ, Harvey JW, Bruss ML (2008). Clinical Biochemistry of Domestic Animals. 6th Ed. Academic Press, Amsterdam. Kontaş T, Salmanoğlu B, Çakmak A, Beşkaya A (2008). Relative changes in serum lipid-lipoprotein and trace element levels in cattle babesiosis. Medycyna Wet, 64, 1104-1106. Kurt D, Yokuş B, Çakır DÜ, Denli O (2008). Investigation levels of certain serum biochemistry components and minerals of pasturing Akkaraman Sheeps in Adiyaman province. Dicle Univ Vet Fak Derg, 1, 34-37. Kutsal A, Alpan O, Arpacık R (1990). İstatistik Uygulamalar. Dizgi-Baskı Basımevi. Ankara. Lacetera N, Bernabucci U, Ronchi B, Nardone A (1999). The effects of injectable sodium selenite on immune function and milk production in Sardinian sheep receiving adequate dietary selenium. Vet Res, 30, 363-370. Man EB, Kartin BL, Durlacher SH, Peters JP (1945). The lipids of serum and liver in patients with hepatic diseases. J Clin Invest, 24 (5), 623-643. Mimioğlu MM, Ulutaş M, Güler S (1973). Yurdumuz Sığırlarında Theileriosis Etkenleri ve Diğer Kan Parazitleri. Ajans-Türk Matbaacılık Sanayii, Ankara. Nizamlıoglu M, Tiftik A M, Turgut Tras B (1991). Kuzuların Beyaz kas hastalığında vitamin E, glutamik okzalasetik transaminaz (GOT), kreatinkinaz (CK) ve Laktat Dehidrogenaz (LDH) aktivitelerinin araştırılması. Doga Tr J Vet and Anim Sci, 15, 59-64. Özer E, Yılmaz K, Erkal N, Şaki E C, Turan T, Angın M, Öztürk G (1995). Bazı Eimeria türleri ile deneysel olarak enfekte edilen erkek akkaraman kuzularda demir ve bakır bağlama kapasitesi. FÜ Sağ Bil Derg, 9 (2), 245-257. Özpınar H, Schweigert FJ, Özpınar A, Wierich M, Senel HS (1988). Aenderung der verteilung der fettlöslichen vitamine auf die lipoproteinfraktionen bei saugkaelbern und kühen in abhaengigkeit von der geburt. Berl, Münch, Tieraerztl, Wschr, 101, 383-387. Philips GB (1960). The lipid composition of serum in patients with liver disease. J Clin Invest, 39, 1639-1650. Rommel M (2000). Protozooenenfektionen der Wiederkaeuer. Eds. M, Rommel J, Eckert E, Kutzer W, Körting T, Schnieder. Veterinarmedizinische Parasitologie. 5, Vollstaending neubearbeitete Auflage. Berlin, Blackwell Wissenschafst-Verlag, p, 121-191. Russel L, Mc Dowell J (1989). Vitamins in Animal Nutrition, Academic Press Inc, San Diego, California. Sato T, Liang K, Vaziri ND (2002). Downregulation of lipoprotein lipase and VLDL receptor in rats with focal glomerulosclerosis. Kidney Int, 61, 157. Singh PP, Barjatiya MK, Dhing S, Bhatnagar R, Kothari S, Dhar V (2001). Evidence suggesting that high intake of fluoride provokes nephrolithiasis in tribal populations. Urol Res, 29 (4), 238- 44. Tanyüksel M, Sayal A, Aydın A (1995). Paraziter hastalıklarda eser elementlerin düzeyleri. Türk Parazitol Derg, 19 (2), 315-321. Valentin A, Rigomier D, Précigout E, Carcy B, Gorenflot A, Schrével (1991). Lipid trafficking between high density lipoproteins and Babesia divergens-infected human erythrocytes. J Biol Cell, 73(1), 63-70.
There are 1 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Yuksel Sengul

Handan Mert

Nihat Mert This is me

Publication Date April 20, 2017
Submission Date July 13, 2016
Acceptance Date August 1, 2016
Published in Issue Year 2017 Volume: 28 Issue: 1

Cite

APA Sengul, Y., Mert, H., & Mert, N. (2017). Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili ve Lipoprotein Düzeylerinin İncelenmesi. Van Veterinary Journal, 28(1), 1-4.
AMA Sengul Y, Mert H, Mert N. Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili ve Lipoprotein Düzeylerinin İncelenmesi. Van Vet J. April 2017;28(1):1-4.
Chicago Sengul, Yuksel, Handan Mert, and Nihat Mert. “Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili Ve Lipoprotein Düzeylerinin İncelenmesi”. Van Veterinary Journal 28, no. 1 (April 2017): 1-4.
EndNote Sengul Y, Mert H, Mert N (April 1, 2017) Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili ve Lipoprotein Düzeylerinin İncelenmesi. Van Veterinary Journal 28 1 1–4.
IEEE Y. Sengul, H. Mert, and N. Mert, “Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili ve Lipoprotein Düzeylerinin İncelenmesi”, Van Vet J, vol. 28, no. 1, pp. 1–4, 2017.
ISNAD Sengul, Yuksel et al. “Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili Ve Lipoprotein Düzeylerinin İncelenmesi”. Van Veterinary Journal 28/1 (April 2017), 1-4.
JAMA Sengul Y, Mert H, Mert N. Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili ve Lipoprotein Düzeylerinin İncelenmesi. Van Vet J. 2017;28:1–4.
MLA Sengul, Yuksel et al. “Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili Ve Lipoprotein Düzeylerinin İncelenmesi”. Van Veterinary Journal, vol. 28, no. 1, 2017, pp. 1-4.
Vancouver Sengul Y, Mert H, Mert N. Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili ve Lipoprotein Düzeylerinin İncelenmesi. Van Vet J. 2017;28(1):1-4.

88x31.png

Accepted papers are licensed under Creative Commons Attribution-NonCommercial 4.0 International License