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T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells

Yıl 2023, Cilt: 44 Sayı: 2, 254 - 257, 30.06.2023

Mustafa Ergül

https://doi.org/10.17776/csj.1238418

Öz

Accumulating evidence reports that T-type calcium channels play crucial roles in tumor formation and development. However, the roles of inhibiting calcium channels in tumor cells with various inhibitors in tumor progression remain unclear. This study aimed to investigate the cytotoxic and apoptotic effects of NNC 55-0396, a T-type calcium channel inhibitor, against SNU-1 gastric cancer cells. Cytotoxic and apoptotic effects of NNC 55-0396 were evaluated by the XTT assay and flow cytometry. The results showed that NNC 55-0396 had concentration-dependent cytotoxicity in SNU-1 cells and its the half-maximal inhibitory concentration (IC50) value was calculated as 4.17 µM. The results of the Annexin V experiments also showed that this inhibitor significantly increased apoptosis in SNU-1 cells. In conclusion, these results demonstrated that NNC 55-0396 induces cytotoxic effects by increasing apoptosis in gastric cancer cells. However, further research is required for its use as a possible therapeutic agent in the treatment of gastric cancer.

Anahtar Kelimeler

Gastric cancer NNC 55-0396 Cytotoxicity Apoptosis.

Kaynakça

  • [1] Smyth E.C., Nilsson M., Grabsch H.I., van Grieken N.C., & Lordick F., Gastric cancer, Lancet (London, England), 396 (10251) (2020) 635–648.
  • [2] Karimi P., Islami F., Anandasabapathy S., Freedman N.D., & Kamangar F., Gastric cancer: descriptive epidemiology, risk factors, screening, and prevention, Cancer Epidemiol Biomarkers Prev., 23 (5) (2014) 700–713.
  • [3] Joshi S.S., Badgwell B.D., Current treatment and recent progress in gastric cancer, CA Cancer J Clin., 71 (3) (2021) 264-279.
  • [4] Harada K., Sakamoto N., Ukai S., Yamamoto Y., Pham Q.T., Taniyama D., Honma R., Maruyama R., Takashima T., Ota H., Takemoto Y., Tanabe K., Ohdan H., & Yasui, W., Establishment of oxaliplatin-resistant gastric cancer organoids: importance of myoferlin in the acquisition of oxaliplatin resistance, Gastric Cancer, 24(6) (2021) 1264–1277.
  • [5] Gonçalves J.C.R., Coulidiati T.H., Monteiro A.L., Carvalho-Gonçalves L.C.T., Valença W.O., Oliveira R.N., Câmara C.A., Araújo D.A.M., Antitumoral activity of novel 1,4-naphthoquinonederivative involves L-type calcium channel activation in human colorectal cancer cell line, Journal of Applied Biomedicine, 14(3) (2016) 229-234.
  • [6] Monteith G.R., Davis F.M., Roberts-Thomson S.J., Calcium channels and pumps in cancer: changes and consequences, J. Biol. Chem., 287(38) (2012) 31666-31673.
  • [7] Wu L., Lin W., Liao Q., Wang H., Lin C., Tang L., Lian W., Chen Z., Li K., Xu L., Zhou R., Ding Y., & Zhao, L., Calcium Channel Blocker Nifedipine Suppresses Colorectal Cancer Progression and Immune Escape by Preventing NFAT2 Nuclear Translocation, Cell Reports, 33(4) (2020) 108327.
  • [8] Phan N.N., Wang C.Y., Chen C.F., Sun Z., Lai M.D., & Lin Y.C., Voltage-gated calcium channels: Novel targets for cancer therapy, Oncology Letters, 14(2) (2017) 2059–2074.
  • [9] Mason R.P., Calcium channel blockers, apoptosis and cancer: is there a biologic relationship?, Journal of the American College of Cardiology, 34(7) (1999) 1857–1866.
  • [10] Huang W., Lu C., Wu Y., Ouyang S., & Chen Y., T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines, J. Exp. Clin. Cancer Res., 34(1) (2015) 54.
  • [11] Ergul M., Bakar-Ates F., A specific inhibitor of polo-like kinase 1, GSK461364A, suppresses proliferation of Raji Burkitt's lymphoma cells through mediating cell cycle arrest, DNA damage, and apoptosis, Chem Biol Interact., 332 (2020) 109288.
  • [12] Dziegielewska B., Gray L.S., Dziegielewski J., T-type calcium channels blockers as new tools in cancer therapies, Pflugers Arch., 466(4) (2014) 801-810.
  • [13] Nam Y., Ryu K.D., Jang C., Moon Y.H., Kim M., Ko D., Chung K.S., Gandini M.A., Lee K. T., Zamponi G.W., & Lee J.Y., Synthesis and cytotoxic effects of 2-thio-3,4-dihydroquinazoline derivatives as novel T-type calcium channel blockers, Bioorganic & Medicinal Chemistry, 28(11) (2020) 115491.
  • [14] Panner A., & Wurster R.D., T-type calcium channels and tumor proliferation, Cell Calcium, 40(2) (2006) 253–259.
  • [15] Das A., Pushparaj C., Herreros J., Nager M., Vilella R., Portero M., Pamplona R., Matias-Guiu X., Martí R.M., & Cantí C., T-type calcium channel blockers inhibit autophagy and promote apoptosis of malignant melanoma cells, Pigment cell & Melanoma Research, 26(6) (2013) 874–885.
  • [16] Valerie N.C., Dziegielewska B., Hosing A.S., Augustin E., Gray L.S., Brautigan D.L., Larner J.M., & Dziegielewski J., Inhibition of T-type calcium channels disrupts Akt signaling and promotes apoptosis in glioblastoma cells, Biochemical Pharmacology, 85(7) (2013) 888–897.
  • [17] Ohkubo T., & Yamazaki J., T-type voltage-activated calcium channel Cav3.1, but not Cav3.2, is involved in the inhibition of proliferation and apoptosis in MCF-7 human breast cancer cells, International Journal of Oncology, 41(1) (2012) 267–275.
  • [18] Granados K., Hüser L., Federico A., Sachindra S., Wolff G., Hielscher T., Novak D., Madrigal-Gambo, V., Sun Q., Vierthaler M., Larribère L., Umansky V., & Utikal J., T-type calcium channel inhibition restores sensitivity to MAPK inhibitors in de-differentiated and adaptive melanoma cells, British Journal of Cancer, 122(7) (2020) 1023–1036.

Yıl 2023, Cilt: 44 Sayı: 2, 254 - 257, 30.06.2023

Mustafa Ergül

https://doi.org/10.17776/csj.1238418

Öz

Anahtar Kelimeler

Gastric cancer NNC 55-0396 Cytotoxicity Apoptosis.

Kaynakça

  • [1] Smyth E.C., Nilsson M., Grabsch H.I., van Grieken N.C., & Lordick F., Gastric cancer, Lancet (London, England), 396 (10251) (2020) 635–648.
  • [2] Karimi P., Islami F., Anandasabapathy S., Freedman N.D., & Kamangar F., Gastric cancer: descriptive epidemiology, risk factors, screening, and prevention, Cancer Epidemiol Biomarkers Prev., 23 (5) (2014) 700–713.
  • [3] Joshi S.S., Badgwell B.D., Current treatment and recent progress in gastric cancer, CA Cancer J Clin., 71 (3) (2021) 264-279.
  • [4] Harada K., Sakamoto N., Ukai S., Yamamoto Y., Pham Q.T., Taniyama D., Honma R., Maruyama R., Takashima T., Ota H., Takemoto Y., Tanabe K., Ohdan H., & Yasui, W., Establishment of oxaliplatin-resistant gastric cancer organoids: importance of myoferlin in the acquisition of oxaliplatin resistance, Gastric Cancer, 24(6) (2021) 1264–1277.
  • [5] Gonçalves J.C.R., Coulidiati T.H., Monteiro A.L., Carvalho-Gonçalves L.C.T., Valença W.O., Oliveira R.N., Câmara C.A., Araújo D.A.M., Antitumoral activity of novel 1,4-naphthoquinonederivative involves L-type calcium channel activation in human colorectal cancer cell line, Journal of Applied Biomedicine, 14(3) (2016) 229-234.
  • [6] Monteith G.R., Davis F.M., Roberts-Thomson S.J., Calcium channels and pumps in cancer: changes and consequences, J. Biol. Chem., 287(38) (2012) 31666-31673.
  • [7] Wu L., Lin W., Liao Q., Wang H., Lin C., Tang L., Lian W., Chen Z., Li K., Xu L., Zhou R., Ding Y., & Zhao, L., Calcium Channel Blocker Nifedipine Suppresses Colorectal Cancer Progression and Immune Escape by Preventing NFAT2 Nuclear Translocation, Cell Reports, 33(4) (2020) 108327.
  • [8] Phan N.N., Wang C.Y., Chen C.F., Sun Z., Lai M.D., & Lin Y.C., Voltage-gated calcium channels: Novel targets for cancer therapy, Oncology Letters, 14(2) (2017) 2059–2074.
  • [9] Mason R.P., Calcium channel blockers, apoptosis and cancer: is there a biologic relationship?, Journal of the American College of Cardiology, 34(7) (1999) 1857–1866.
  • [10] Huang W., Lu C., Wu Y., Ouyang S., & Chen Y., T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines, J. Exp. Clin. Cancer Res., 34(1) (2015) 54.
  • [11] Ergul M., Bakar-Ates F., A specific inhibitor of polo-like kinase 1, GSK461364A, suppresses proliferation of Raji Burkitt's lymphoma cells through mediating cell cycle arrest, DNA damage, and apoptosis, Chem Biol Interact., 332 (2020) 109288.
  • [12] Dziegielewska B., Gray L.S., Dziegielewski J., T-type calcium channels blockers as new tools in cancer therapies, Pflugers Arch., 466(4) (2014) 801-810.
  • [13] Nam Y., Ryu K.D., Jang C., Moon Y.H., Kim M., Ko D., Chung K.S., Gandini M.A., Lee K. T., Zamponi G.W., & Lee J.Y., Synthesis and cytotoxic effects of 2-thio-3,4-dihydroquinazoline derivatives as novel T-type calcium channel blockers, Bioorganic & Medicinal Chemistry, 28(11) (2020) 115491.
  • [14] Panner A., & Wurster R.D., T-type calcium channels and tumor proliferation, Cell Calcium, 40(2) (2006) 253–259.
  • [15] Das A., Pushparaj C., Herreros J., Nager M., Vilella R., Portero M., Pamplona R., Matias-Guiu X., Martí R.M., & Cantí C., T-type calcium channel blockers inhibit autophagy and promote apoptosis of malignant melanoma cells, Pigment cell & Melanoma Research, 26(6) (2013) 874–885.
  • [16] Valerie N.C., Dziegielewska B., Hosing A.S., Augustin E., Gray L.S., Brautigan D.L., Larner J.M., & Dziegielewski J., Inhibition of T-type calcium channels disrupts Akt signaling and promotes apoptosis in glioblastoma cells, Biochemical Pharmacology, 85(7) (2013) 888–897.
  • [17] Ohkubo T., & Yamazaki J., T-type voltage-activated calcium channel Cav3.1, but not Cav3.2, is involved in the inhibition of proliferation and apoptosis in MCF-7 human breast cancer cells, International Journal of Oncology, 41(1) (2012) 267–275.
  • [18] Granados K., Hüser L., Federico A., Sachindra S., Wolff G., Hielscher T., Novak D., Madrigal-Gambo, V., Sun Q., Vierthaler M., Larribère L., Umansky V., & Utikal J., T-type calcium channel inhibition restores sensitivity to MAPK inhibitors in de-differentiated and adaptive melanoma cells, British Journal of Cancer, 122(7) (2020) 1023–1036.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri
Bölüm Natural Sciences
Yazarlar

Mustafa Ergül 0000-0003-4303-2996

Yayımlanma Tarihi 30 Haziran 2023
Gönderilme Tarihi 18 Ocak 2023
Kabul Tarihi 7 Haziran 2023
Yayımlandığı Sayı Yıl 2023Cilt: 44 Sayı: 2

Kaynak Göster

APA Ergül, M. (2023). T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells. Cumhuriyet Science Journal, 44(2), 254-257. https://doi.org/10.17776/csj.1238418