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The Evaluation of ANA and dsDNA results

Yıl 2015, Cilt: 20 Sayı: 1, 7 - 10, 02.06.2015

Öz

Detection of auto-antibody by Enzyme Linked Immunosorbent Assay (ELISA) technique is used method for diagnosis of autoimmune disease.  This study was aimed to compare the measurement of antinuclear antibodies (ANA) and double-stranded DNA (ds DNA) by ELISA in serum samples.

Serum samples were obtained from 2 sources of: sent to the laboratory for ANA testing and sent to the laboratory for dsDNA. Each of these sera was tested for the presence of ANA and dsDNA by ELISA kits. This tests were performed by commercial ‘‘Aeskulisa dsDNA check” (AESKU DIAGNOSTICS, GERMANY) according to the manufacturer's instructions. ANA and ds DNA results were classified as positive or negative for each patient. Borderline results were arbitrarily classified as positive.

In patients, 2,96% (58/1975) were ANA positive and 4,52% (29/642) were ds DNA positive by ELISA. There is no significant correlation between ANA and ds DNA positivity. Only two patient both ANA and ds DNA positivity were found by ELISA kits. Agreement between assays is generally marginal.

There are a number of factors which may contribute to the variability between these assays. The ELISA ANA assay in our laboratory uses a commercial kit utilize an IgG-specifik second antibody. Finally, each of the assays provides different criteria for clinicians and ANA screen test should be followed up with a fluorescent tests to provide information on ANA pattern.

Kaynakça

  • Takeuchi Y, Ishikawa O, Miyachi Y. The comparative
  • study of anti-double stranded DNA antibody levels
  • measured by radioimmunoassay and enzyme-linked immunosorbent assay in systemic lupus erythematosus. J Dermatol 1997; 24: 297-300.
  • Krippner H, Merle S, Pirlet K. Enzyme immunoassay for IgG and IgM antibodies against dsDNA and ssDNA. Z Rheumatol 1983; 42: 256-260.
  • Ward MM. Laboratory testing for systemic rheumatic diseases. Postgrad Med 1998; 103: 93-100.
  • Şimşek B, Mısırlıoğlu H, Ören S, et al. Retrospektif olarak Antinükleer Antikor Pozitif olan hastalarda indirect immunofloresan (İİF) ve İmmünoblotting (İB) test sonuçlarının değerlendirilmesi. 21. Ulusal İmmünoloji Kongresi 2011; 21: 111.
  • İlhan F, Akbulut H, Gödekmerdan A. Laboratuvarımızda saptanan ANA paternlerinin retrospektif incelenmesi. 21. İmmünoloji Kongresi 2011; 21: 126.
  • Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch Pathol Lab Med 2000; 124: 71- 81.
Yıl 2015, Cilt: 20 Sayı: 1, 7 - 10, 02.06.2015

Öz

Kaynakça

  • Takeuchi Y, Ishikawa O, Miyachi Y. The comparative
  • study of anti-double stranded DNA antibody levels
  • measured by radioimmunoassay and enzyme-linked immunosorbent assay in systemic lupus erythematosus. J Dermatol 1997; 24: 297-300.
  • Krippner H, Merle S, Pirlet K. Enzyme immunoassay for IgG and IgM antibodies against dsDNA and ssDNA. Z Rheumatol 1983; 42: 256-260.
  • Ward MM. Laboratory testing for systemic rheumatic diseases. Postgrad Med 1998; 103: 93-100.
  • Şimşek B, Mısırlıoğlu H, Ören S, et al. Retrospektif olarak Antinükleer Antikor Pozitif olan hastalarda indirect immunofloresan (İİF) ve İmmünoblotting (İB) test sonuçlarının değerlendirilmesi. 21. Ulusal İmmünoloji Kongresi 2011; 21: 111.
  • İlhan F, Akbulut H, Gödekmerdan A. Laboratuvarımızda saptanan ANA paternlerinin retrospektif incelenmesi. 21. İmmünoloji Kongresi 2011; 21: 126.
  • Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch Pathol Lab Med 2000; 124: 71- 81.
Toplam 8 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Articles
Yazarlar

Engın Karakece Bu kişi benim

İhsan Cıftcı

Alı Atasoy

Yayımlanma Tarihi 2 Haziran 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 20 Sayı: 1

Kaynak Göster

APA Karakece, E., Cıftcı, İ., & Atasoy, A. (2015). The Evaluation of ANA and dsDNA results. EASTERN JOURNAL OF MEDICINE, 20(1), 7-10.
AMA Karakece E, Cıftcı İ, Atasoy A. The Evaluation of ANA and dsDNA results. EASTERN JOURNAL OF MEDICINE. Haziran 2015;20(1):7-10.
Chicago Karakece, Engın, İhsan Cıftcı, ve Alı Atasoy. “The Evaluation of ANA and DsDNA Results”. EASTERN JOURNAL OF MEDICINE 20, sy. 1 (Haziran 2015): 7-10.
EndNote Karakece E, Cıftcı İ, Atasoy A (01 Haziran 2015) The Evaluation of ANA and dsDNA results. EASTERN JOURNAL OF MEDICINE 20 1 7–10.
IEEE E. Karakece, İ. Cıftcı, ve A. Atasoy, “The Evaluation of ANA and dsDNA results”, EASTERN JOURNAL OF MEDICINE, c. 20, sy. 1, ss. 7–10, 2015.
ISNAD Karakece, Engın vd. “The Evaluation of ANA and DsDNA Results”. EASTERN JOURNAL OF MEDICINE 20/1 (Haziran 2015), 7-10.
JAMA Karakece E, Cıftcı İ, Atasoy A. The Evaluation of ANA and dsDNA results. EASTERN JOURNAL OF MEDICINE. 2015;20:7–10.
MLA Karakece, Engın vd. “The Evaluation of ANA and DsDNA Results”. EASTERN JOURNAL OF MEDICINE, c. 20, sy. 1, 2015, ss. 7-10.
Vancouver Karakece E, Cıftcı İ, Atasoy A. The Evaluation of ANA and dsDNA results. EASTERN JOURNAL OF MEDICINE. 2015;20(1):7-10.