Farklı Cihazlarda İyon Seçici Elektrot (ISE) Yöntemi ile Ölçülen Sodyum, Potasyum ve Klor Sonuçlarının Kıyaslanması
Öz
Amaç: Elektrolit ölçümleri, klinik biyokimya laboratuvarlarında yapılan rutin testlerin vazgeçilmez bir parçasıdır. Bu çalışmada, serum sodyum (Na), potasyum (K) ve klor (Cl) elektrolitleri iyon seçici elektrot (ISE) yöntemi ile ölçülmüş ve doğrusallık, kesinlik, tekrarlanabilirlik ve doğruluk seviyeleri Mindray BS-800M ve Beckman Coulter AU5800 cihazlarında değerlendirilmesi amaçlanmaktadır.
Gereç ve yöntem: Serum örnekleri, Ocak-Nisan 2021 tarihleri arasında Karapınar Devlet Hastanesi'ne kontrol amaçlı başvuran sağlıklı gönüllülerden alınmıştır. Örnek probu, ISE yöntemi için ayrı bir numune probu olup Mindray BS-800M ve Beckman Coulter AU5800 cihazlarında indirekt yöntem kullanılarak gerçekleştirilmiştir.
Bulgular: Karşılaştırmalı ölçümler farklı cihazlarda yapılırken elektrolitler arasındaki korelasyonlar Na [r=0,964, % 95 CI (0,52-0,90)], K [r=0,995, % 95 CI (0,09-) 0.12)] ve Cl [r=0.972, % 95 CI (0.69-1.12)] olarak bulundu. Ayrıca bu sonuçlar arasında da bir korelasyon belirlenmiştir.
Sonuç: Farklı analizörlerde ölçülen Na, K ve Cl elektrolitleri arasında bir tutarlılık belirlendi. Ayrıca, bu elektrolitler arasında pozitif bir korelasyon bulunmaktadır. Bu sonuçların açıklığa kavuşturulması için daha fazla ve kapsamlı çalışmalara ihtiyaç olduğu görülmektedir.
Anahtar Kelimeler
Destekleyen Kurum
Yok
Proje Numarası
-
Kaynakça
- 1- Arampatzis S, Funk GC, Leichtle AB, et al. Impact of diuretic therapy-associated electrolyte disorders present on admission to the emergency department: A cross-sectional analysis. BMC Med 2013;11:83.
- 2- Lee JW. Fluid and electrolyte disturbances in critically ill patients. Electrolyte Blood Press 2010;8(2):72-81.
- 3- Tietz, NW. editor. Fundamentals of Clinical Chemistry. 3rd Edition, W.B. Saunders, 1987.
- 4- Yilmaz S, Uysal HB, Avcil M, et al. Comparison of different methods for measurement of electrolytes in patients admitted to the intensive care unit. Saudi Med J 2016;37(3):262-7.
- 5- Morf WE, Pretsch E, de Rooij NF. Memory Effects of Ion-Selective Electrodes: Theory and Computer Simulation of the Time-Dependent Potential Response to Multiple Sample Changes. J Electroanal Chem (Lausanne) 2009;633(1):137-145.
- 6- Türkalp I, Erbiz E. İyon selektif elektrod ile serumda sodyum ve potasyum tayini-flame fotometrisi ile kıyaslanması, Kartal Eğitim ve Araştırma Klinikleri Dergisi 1990;1(3):162-66.
- 7- Ustundağ Y, Huysal K, Ozgunay ŞE, et al. Interchangeability of Sodium and Potassium Result Values of Arterial Blood Gas with Laboratory Analyzer: Narrative Review. Indian J Crit Care Med 2019;23(1):35-42.
- 8- Guenat OT, Generelli S, de Rooij NF, et al. Development of an array of ion-selective microelectrodes aimed for the monitoring of extracellular ionic activities. Anal Chem 2006;78(21):7453-60.
- 9- Banerjee A, Mehrotra G. Role of Correction Factor in Minimizing Errors While Calculating Electrolyte Values between Blood-gas Analyzer and Laboratory Autoanalyzer: A Comparative Study. Indian J Crit Care Med 2018;22(1):34-39.
- 10- Jia KK, Zhang J. Evaluation of five routine glucose methods on an Olympus AU5400 analyzer using the CDC hexokinase reference method. Clin Chem Lab Med 2010;48:361-4.
- 11- Gross P. Correction of hyponatremia. Semin Nephrol 2001;21:269-272.
- 12- Adrogue HJ. Consequences of inadequate management of hyponatremia. Am J Nephrol 2005;25:240-249.
- 13- Adrogué HJ, Madias NE. Hypernatremia. N Engl J Med 2000;342(20):1493-9.
- 14- Aygencel G. Potasyum Metabolizması Bozuklukları. Yoğun Bakım Dergisi 2018;12(1):31-42.
- 15- Morrison G. Serum Chloride. In: Walker HK, Hall WD, Hurst JW, editors. Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd edition. Boston: Butterworths, 1990. Chapter 197.
- 16- Hanberg JS, Rao V, Ter Maaten JM, et al. Hypochloremia and Diuretic Resistance in Heart Failure: Mechanistic Insights. Circ Heart Fail 2016;9(8):10.
- 17- Ustundag-Budak Y, Huysal K. Application of Sigma Metrics and Performance Comparison Between Two Biochemistry Analyser and a Blood Gas Analyser for the Determination of Electrolytes. J Clin Diagn Res 2017;11(2):BC06-BC09.
- 18- Lippi G, Dipalo M, Musa R, et al. Evaluation of the analytical performances of the novel Beckman Coulter AU5800. Clin Biochem 2012;45(6):502-4.
Comparison of the Results of Sodium, Potassium, and Chlorine Measured Through Ion Selective Electrode (ISE) Method in Different Devices
Öz
Objective: The measurements of electrolytes are an indispensable part of the routine tests performed in clinical biochemistry laboratories. In the present study, we aimed to measure serum sodium (Na), potassium (K), and chlorine (Cl) electrolytes via the ion-selective electrode (ISE) method and evaluated in terms of linearity, precision, repeatability, and accuracy levels in the Mindray BS-800M and Beckman Coulter AU5800 devices.
Material and methods: The serum samples were obtained from the healthy volunteers admitted to the Karapınar State Hospital for control purposes between January and April 2021. The sample probe was conducted using the same indirect method in the Mindray BS-800M and Beckman Coulter AU5800 devices, which is an individual sample probe for the ISE method.
Results: While the comparative measurements were performed on different devices, the correlations between the electrolytes were found as follows: Na [r=0.964, 95% CI (0.52-0.90)], K [r=0.995, 95% CI (0.09-0.12)], and Cl [r=0.972, 95% CI (0.69-1.12)]. Moreover, a correlation was also found between these results.
Conclusion: A consistency between the measured Na, K, and Cl electrolytes was determined on the different analyzers. Also, a positive correlation was determined between those electrolytes. We consider that further and comprehensive studies are needed to elucidate these results.
Anahtar Kelimeler
Proje Numarası
-
Kaynakça
- 1- Arampatzis S, Funk GC, Leichtle AB, et al. Impact of diuretic therapy-associated electrolyte disorders present on admission to the emergency department: A cross-sectional analysis. BMC Med 2013;11:83.
- 2- Lee JW. Fluid and electrolyte disturbances in critically ill patients. Electrolyte Blood Press 2010;8(2):72-81.
- 3- Tietz, NW. editor. Fundamentals of Clinical Chemistry. 3rd Edition, W.B. Saunders, 1987.
- 4- Yilmaz S, Uysal HB, Avcil M, et al. Comparison of different methods for measurement of electrolytes in patients admitted to the intensive care unit. Saudi Med J 2016;37(3):262-7.
- 5- Morf WE, Pretsch E, de Rooij NF. Memory Effects of Ion-Selective Electrodes: Theory and Computer Simulation of the Time-Dependent Potential Response to Multiple Sample Changes. J Electroanal Chem (Lausanne) 2009;633(1):137-145.
- 6- Türkalp I, Erbiz E. İyon selektif elektrod ile serumda sodyum ve potasyum tayini-flame fotometrisi ile kıyaslanması, Kartal Eğitim ve Araştırma Klinikleri Dergisi 1990;1(3):162-66.
- 7- Ustundağ Y, Huysal K, Ozgunay ŞE, et al. Interchangeability of Sodium and Potassium Result Values of Arterial Blood Gas with Laboratory Analyzer: Narrative Review. Indian J Crit Care Med 2019;23(1):35-42.
- 8- Guenat OT, Generelli S, de Rooij NF, et al. Development of an array of ion-selective microelectrodes aimed for the monitoring of extracellular ionic activities. Anal Chem 2006;78(21):7453-60.
- 9- Banerjee A, Mehrotra G. Role of Correction Factor in Minimizing Errors While Calculating Electrolyte Values between Blood-gas Analyzer and Laboratory Autoanalyzer: A Comparative Study. Indian J Crit Care Med 2018;22(1):34-39.
- 10- Jia KK, Zhang J. Evaluation of five routine glucose methods on an Olympus AU5400 analyzer using the CDC hexokinase reference method. Clin Chem Lab Med 2010;48:361-4.
- 11- Gross P. Correction of hyponatremia. Semin Nephrol 2001;21:269-272.
- 12- Adrogue HJ. Consequences of inadequate management of hyponatremia. Am J Nephrol 2005;25:240-249.
- 13- Adrogué HJ, Madias NE. Hypernatremia. N Engl J Med 2000;342(20):1493-9.
- 14- Aygencel G. Potasyum Metabolizması Bozuklukları. Yoğun Bakım Dergisi 2018;12(1):31-42.
- 15- Morrison G. Serum Chloride. In: Walker HK, Hall WD, Hurst JW, editors. Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd edition. Boston: Butterworths, 1990. Chapter 197.
- 16- Hanberg JS, Rao V, Ter Maaten JM, et al. Hypochloremia and Diuretic Resistance in Heart Failure: Mechanistic Insights. Circ Heart Fail 2016;9(8):10.
- 17- Ustundag-Budak Y, Huysal K. Application of Sigma Metrics and Performance Comparison Between Two Biochemistry Analyser and a Blood Gas Analyser for the Determination of Electrolytes. J Clin Diagn Res 2017;11(2):BC06-BC09.
- 18- Lippi G, Dipalo M, Musa R, et al. Evaluation of the analytical performances of the novel Beckman Coulter AU5800. Clin Biochem 2012;45(6):502-4.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Klinik Tıp Bilimleri |
| Bölüm | Original Article |
| Yazarlar | |
| Proje Numarası | - |
| Yayımlanma Tarihi | 29 Nisan 2022 |
| Gönderilme Tarihi | 17 Aralık 2021 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 32 Sayı: 2 |
Genel Tıp Dergisi Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.