Araştırma Makalesi
BibTex RIS Kaynak Göster

In-vitro cytotoxicity of white vinegar: Effects of different concentrations

Yıl 2022, Cilt: 4 Sayı: 1, 18 - 24, 30.04.2022

Arzu Zeynep Yıldırım Şükran Yılmaz Nuray Bağcı İlkay Peker

Öz

Aim: We compared the cytotoxic effects of different concentration of white vinegar, that easy to apply, unexpensive and effective on microorganisms.
Method: The cells L929 mouse skin fibroblasts were used for the experiments. Cytotoxicity was evaluated colourimetrically by MTT [4,5-dimethylthiazol-2-yl-2,5 diphenyltetrazolium bromide; thiazolyl blue] proliferation assay.
Results: White vinegar was found to be moderately cytotoxic at its all concentrations, in the 10-minutes incubation period. While the other concentrations were moderately cytotoxic, only 100% white vinegar concentration was found to be severely cytotoxic, in the 24-hours incubation period.
Conclusion: It was observed that white vinegar was moderately cytotoxic on L929 fibroblast at all concentrations, in the 10-minutes incubation period

Anahtar Kelimeler

white vinegar cytotoxicity cell viability

Kaynakça

  • Basson, N., A. Quick & C. Thomas. (1992). Household products as sanitising agents in denture cleansing. The Journal of the Dental Association of South Africa= Die Tydskrif van die Tandheelkundige Vereniging van Suid-afrika, 47(10), 437-439.
  • Berridge, M.V., P.M. Herst & A.S. Tan. (2005). Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnology Annual Review, 11, 127-152.
  • Coelho, C., Y. Sousa & A. Dare. (2004). Denture‐related oral mucosal lesions in a Brazilian school of dentistry. Journal of oral rehabilitation, 31(2), 135-139.
  • Davenport, J.C. (1972). The denture surface. British Dental Journal, 133(3), 101-105.
  • Da Silva, F. C., Kimpara, E. T., Mancini, M. N. G., Balducci, I., Jorge, A. O. C., & Koga‐Ito, C. Y. (2008). Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. Journal of Prosthodontics: Implant, Esthetic and Reconstructive Dentistry, 17(8), 627-633.
  • DeWald, J.P. (1997). The use of extracted teeth for in vitro bonding studies: a review of infection control considerations. Dental Materials, 13(2), 74-81.
  • Dominici, J. T., Eleazer, P. D., Clark, S. J., Staat, R. H., & Scheetz, J. P. (2001). Disinfection/sterilization of extracted teeth for dental student use. Journal of Dental Education, 65(11), 1278-1281.
  • Estrela, C., Holland, R., Bernabé, P. F. E., Souza, V. D., & Estrela, C. R. (2004). Antimicrobial potential of medicaments used in healing process in dogs' teeth with apical periodontitis. Brazilian Dental Journal, 15(3), 181-185.
  • Jagger, D. & A. Harrison. (1995). Denture cleansing--the best approach. British Dental Journal, 178(11), 413-417.
  • Kulak, Y., A. Arikan & E. Kazazoglu. (1997). Existence of Candida albicans and microorganisms in denture stomatitis patients. Journal of Oral Rehabilitation, 24(10), 788-790.
  • Le Bars, P., Kouadio, A. A., N’goran, J. K., Badran, Z., & Soueidan, A. (2015). Relationship between removable prosthesis and some systemics disorders. The Journal of the Indian Prosthodontic Society, 15(4), 292.
  • Lyon, J. P., da Costa, S. C., Totti, V. M. G., Munhoz, M. F. V., & de Resende, M. A. (2006). Predisposing conditions for Candida spp. carriage in the oral cavity of denture wearers and individuals with natural teeth. Canadian Journal of Microbiology, 52(5), 462-467.
  • Lefebvre, C.A., K.L. Knoernschild & G.S. Schuster. (1994). Cytotoxicity of eluates from light-polymerized denture base resins. The Journal of Prosthetic Dentistry, 72(6), 644-650.
  • Lefebvre, C.A. & G.S. Schuster. (1994). Biocompatibility of visible light-cured resin systems in prosthodontics. Journal of Prosthetic Dentistry, 71(2), 178-185.
  • Meriç, G., J.E. Dahl & I.E. Ruyter. (2008). Cytotoxicity of silica–glass fiber reinforced composites. Dental Materials, 24(9), 1201-1206.
  • Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63.
  • Nakamoto, K., M. Tamamoto & T. Hamada. (1991). Evaluation of denture cleansers with and without enzymes against Candida albicans. The Journal of Prosthetic Dentistry, 66(6), 792-795.
  • Nascimento, M. S., Silva, N., Catanozi, M. P. L. M., & Silva, K. C. (2003). Effects of different disinfection treatments on the natural microbiota of lettuce. Journal of Food Protection, 66(9), 1697-1700.
  • Palenik, C.J. & C.H. Miller. (1984). In vitro testing of three denture-cleaning systems. The Journal of Prosthetic Dentistry, 51(6), 751-754.
  • Preshaw, P. M., Walls, A. W. G., Jakubovics, N. S., Moynihan, P. J., Jepson, N. J. A., & Loewy, Z. (2011). Association of removable partial denture use with oral and systemic health. Journal of Dentistry, 39(11), 711-719.
  • Pinto, T. M. S., Neves, A. C. C., Leão, M. V. P., & Jorge, A. O. C. (2008). Vinegar as an antimicrobial agent for control of Candida spp. in complete denture wearers. Journal of Applied Oral Science, 16, 385-390.
  • Ribeiro, D. G., Pavarina, A. C., Dovigo, L. N., Machado, A. L., Giampaolo, E. T., & Vergani, C. E. (2012). Prevalence of Candida spp. associated with bacteria species on complete dentures. Gerodontology, 29(3), 203-208.
  • Riss, T. L., Moravec, R. A., Niles, A. L., Duellman, S., Benink, H. A., Worzella, T. J., & Minor, L. (2016). Cell viability assays. Assay Guidance Manual.
  • Salles, A. E. S., Macedo, L. D., Fernandes, R. A., Silva‐Lovato, C. H., & Paranhos, H. D. F. (2007). Comparative analysis of biofilm levels in complete upper and lower dentures after brushing associated with specific denture paste and neutral soap. Gerodontology, 24(4), 217-223.
  • Schou, L., C. Wight, and C. Cumming. (1987). Oral hygiene habits, denture plaque, presence of yeasts and stomatitis in institutionalized elderly in Lathian, Scotland. Community dentistry and oral epidemiology, 15(2), 85-89.
  • Suresan, V., Mantri, S., Deogade, S., Sumathi, K., Panday, P., Galav, A., & Mishra, K. (2016). Denture hygiene knowledge, attitudes, and practices toward patient education in denture care among dental practitioners of Jabalpur city, Madhya Pradesh, India. The Journal of the Indian Prosthodontic Society, 16(1), 30.
  • Tijare, M., Smitha, D., Kasetty, S., Kallianpur, S., Gupta, S., & Amith, H. V. (2014). Vinegar as a disinfectant of extracted human teeth for dental educational use. Journal of Oral and Maxillofacial Pathology, 18(1), 14.
  • Ünlü, A., O.T. Altay & S. Sahmali. (1996). The role of denture cleansers on the whitening of acrylic resins. International Journal of Prosthodontics, 9(3).
  • Vijayakumar, C. & C.E. Wolf-Hall. (2002a). Evaluation of household sanitizers for reducing levels of Escherichia coli on iceberg lettuce. Journal of Food Protection,. 65(10), 1646-1650.
  • Vijayakumar, C. & C. Wolf-Hall. (2002b). Minimum bacteriostatic and bactericidal concentrations of household sanitizers for Escherichia coli strains in tryptic soy broth. Food Microbiology, 19(4), 383-388.
  • Viana, P. S., Machado, A. L., Giampaolo, E. T., Pavarina, A. C., & Vergani, C. E. (2010). Disinfection of bovine enamel by microwave irradiation: effect on the surface microhardness and demineralization/remineralization processes. Caries Research, 44(4), 349-357.
  • Yang, Y., Zhang, H., Chai, Z., Chen, J., & Zhang, S. (2014). Multiple logistic regression analysis of risk factors associated with denture plaque and staining in chinese removable denture wearers over 40 years old in xi’an–a cross-sectional study. PLoS One, 9(2), e87749.
  • Yildirim-Bicer, A. Z., Peker, I., Akca, G., & Celik, I. (2014). In vitro antifungal evaluation of seven different disinfectants on acrylic resins. BioMed Research International

Yıl 2022, Cilt: 4 Sayı: 1, 18 - 24, 30.04.2022

Arzu Zeynep Yıldırım Şükran Yılmaz Nuray Bağcı İlkay Peker

Öz

Kaynakça

  • Basson, N., A. Quick & C. Thomas. (1992). Household products as sanitising agents in denture cleansing. The Journal of the Dental Association of South Africa= Die Tydskrif van die Tandheelkundige Vereniging van Suid-afrika, 47(10), 437-439.
  • Berridge, M.V., P.M. Herst & A.S. Tan. (2005). Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnology Annual Review, 11, 127-152.
  • Coelho, C., Y. Sousa & A. Dare. (2004). Denture‐related oral mucosal lesions in a Brazilian school of dentistry. Journal of oral rehabilitation, 31(2), 135-139.
  • Davenport, J.C. (1972). The denture surface. British Dental Journal, 133(3), 101-105.
  • Da Silva, F. C., Kimpara, E. T., Mancini, M. N. G., Balducci, I., Jorge, A. O. C., & Koga‐Ito, C. Y. (2008). Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. Journal of Prosthodontics: Implant, Esthetic and Reconstructive Dentistry, 17(8), 627-633.
  • DeWald, J.P. (1997). The use of extracted teeth for in vitro bonding studies: a review of infection control considerations. Dental Materials, 13(2), 74-81.
  • Dominici, J. T., Eleazer, P. D., Clark, S. J., Staat, R. H., & Scheetz, J. P. (2001). Disinfection/sterilization of extracted teeth for dental student use. Journal of Dental Education, 65(11), 1278-1281.
  • Estrela, C., Holland, R., Bernabé, P. F. E., Souza, V. D., & Estrela, C. R. (2004). Antimicrobial potential of medicaments used in healing process in dogs' teeth with apical periodontitis. Brazilian Dental Journal, 15(3), 181-185.
  • Jagger, D. & A. Harrison. (1995). Denture cleansing--the best approach. British Dental Journal, 178(11), 413-417.
  • Kulak, Y., A. Arikan & E. Kazazoglu. (1997). Existence of Candida albicans and microorganisms in denture stomatitis patients. Journal of Oral Rehabilitation, 24(10), 788-790.
  • Le Bars, P., Kouadio, A. A., N’goran, J. K., Badran, Z., & Soueidan, A. (2015). Relationship between removable prosthesis and some systemics disorders. The Journal of the Indian Prosthodontic Society, 15(4), 292.
  • Lyon, J. P., da Costa, S. C., Totti, V. M. G., Munhoz, M. F. V., & de Resende, M. A. (2006). Predisposing conditions for Candida spp. carriage in the oral cavity of denture wearers and individuals with natural teeth. Canadian Journal of Microbiology, 52(5), 462-467.
  • Lefebvre, C.A., K.L. Knoernschild & G.S. Schuster. (1994). Cytotoxicity of eluates from light-polymerized denture base resins. The Journal of Prosthetic Dentistry, 72(6), 644-650.
  • Lefebvre, C.A. & G.S. Schuster. (1994). Biocompatibility of visible light-cured resin systems in prosthodontics. Journal of Prosthetic Dentistry, 71(2), 178-185.
  • Meriç, G., J.E. Dahl & I.E. Ruyter. (2008). Cytotoxicity of silica–glass fiber reinforced composites. Dental Materials, 24(9), 1201-1206.
  • Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63.
  • Nakamoto, K., M. Tamamoto & T. Hamada. (1991). Evaluation of denture cleansers with and without enzymes against Candida albicans. The Journal of Prosthetic Dentistry, 66(6), 792-795.
  • Nascimento, M. S., Silva, N., Catanozi, M. P. L. M., & Silva, K. C. (2003). Effects of different disinfection treatments on the natural microbiota of lettuce. Journal of Food Protection, 66(9), 1697-1700.
  • Palenik, C.J. & C.H. Miller. (1984). In vitro testing of three denture-cleaning systems. The Journal of Prosthetic Dentistry, 51(6), 751-754.
  • Preshaw, P. M., Walls, A. W. G., Jakubovics, N. S., Moynihan, P. J., Jepson, N. J. A., & Loewy, Z. (2011). Association of removable partial denture use with oral and systemic health. Journal of Dentistry, 39(11), 711-719.
  • Pinto, T. M. S., Neves, A. C. C., Leão, M. V. P., & Jorge, A. O. C. (2008). Vinegar as an antimicrobial agent for control of Candida spp. in complete denture wearers. Journal of Applied Oral Science, 16, 385-390.
  • Ribeiro, D. G., Pavarina, A. C., Dovigo, L. N., Machado, A. L., Giampaolo, E. T., & Vergani, C. E. (2012). Prevalence of Candida spp. associated with bacteria species on complete dentures. Gerodontology, 29(3), 203-208.
  • Riss, T. L., Moravec, R. A., Niles, A. L., Duellman, S., Benink, H. A., Worzella, T. J., & Minor, L. (2016). Cell viability assays. Assay Guidance Manual.
  • Salles, A. E. S., Macedo, L. D., Fernandes, R. A., Silva‐Lovato, C. H., & Paranhos, H. D. F. (2007). Comparative analysis of biofilm levels in complete upper and lower dentures after brushing associated with specific denture paste and neutral soap. Gerodontology, 24(4), 217-223.
  • Schou, L., C. Wight, and C. Cumming. (1987). Oral hygiene habits, denture plaque, presence of yeasts and stomatitis in institutionalized elderly in Lathian, Scotland. Community dentistry and oral epidemiology, 15(2), 85-89.
  • Suresan, V., Mantri, S., Deogade, S., Sumathi, K., Panday, P., Galav, A., & Mishra, K. (2016). Denture hygiene knowledge, attitudes, and practices toward patient education in denture care among dental practitioners of Jabalpur city, Madhya Pradesh, India. The Journal of the Indian Prosthodontic Society, 16(1), 30.
  • Tijare, M., Smitha, D., Kasetty, S., Kallianpur, S., Gupta, S., & Amith, H. V. (2014). Vinegar as a disinfectant of extracted human teeth for dental educational use. Journal of Oral and Maxillofacial Pathology, 18(1), 14.
  • Ünlü, A., O.T. Altay & S. Sahmali. (1996). The role of denture cleansers on the whitening of acrylic resins. International Journal of Prosthodontics, 9(3).
  • Vijayakumar, C. & C.E. Wolf-Hall. (2002a). Evaluation of household sanitizers for reducing levels of Escherichia coli on iceberg lettuce. Journal of Food Protection,. 65(10), 1646-1650.
  • Vijayakumar, C. & C. Wolf-Hall. (2002b). Minimum bacteriostatic and bactericidal concentrations of household sanitizers for Escherichia coli strains in tryptic soy broth. Food Microbiology, 19(4), 383-388.
  • Viana, P. S., Machado, A. L., Giampaolo, E. T., Pavarina, A. C., & Vergani, C. E. (2010). Disinfection of bovine enamel by microwave irradiation: effect on the surface microhardness and demineralization/remineralization processes. Caries Research, 44(4), 349-357.
  • Yang, Y., Zhang, H., Chai, Z., Chen, J., & Zhang, S. (2014). Multiple logistic regression analysis of risk factors associated with denture plaque and staining in chinese removable denture wearers over 40 years old in xi’an–a cross-sectional study. PLoS One, 9(2), e87749.
  • Yildirim-Bicer, A. Z., Peker, I., Akca, G., & Celik, I. (2014). In vitro antifungal evaluation of seven different disinfectants on acrylic resins. BioMed Research International
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Diş Hekimliği
Bölüm Makaleler
Yazarlar

Arzu Zeynep Yıldırım 0000-0002-9332-8982

Şükran Yılmaz 0000-0002-7945-1124

Nuray Bağcı 0000-0001-9362-723X

İlkay Peker 0000-0002-2888-2979

Yayımlanma Tarihi 30 Nisan 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 4 Sayı: 1

Kaynak Göster

APA Yıldırım, A. Z., Yılmaz, Ş., Bağcı, N., Peker, İ. (2022). In-vitro cytotoxicity of white vinegar: Effects of different concentrations. Journal of Gazi University Health Sciences Institute, 4(1), 18-24.
 Kapak Resmi İndir

22691
https://www.atifdizini.com/