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The Effects of Medicinal Plants on Cancer Cell Lines and Efficacy of Experimental Animal Model

Yıl 2018, Cilt: 5 Sayı: 1, 49 - 59, 04.01.2018
https://doi.org/10.21448/ijsm.365065

Öz

The use of medicinal plants as an alternative treatment is a historical
process and has been known for a long time so that classical treatment can be
more effective in wound and cancer treatment. The purpose of this study
was to investigate the effects of plant extracts used for therapeutic purposes
in cancer cell lines in vitro wound model and in vivo experimental animal model
in order to obtain this information. The plants, olive oil (Oleocanthal),
mistletoe (Viscum album), Common
Centaury (Centaurium erythraea), Momordica charantia, Inula viscosa, Citrus
aurantium
, Thyme oil (Thymus vulgaris) and algae (Jania longifurca), were used. MCF-7, MB-MDA-231, 67NR and 4T1 for
breast, NB2a for neuron, L929 for fibroblast and normal somatic mesenchymal
stem cell for comparison were selected for in vitro wound models. As an in vivo
breast cancer model, female Balb/c mice were injected with 4T1 cells and skin
wound healing in rats was investigated. The effects of medicinal plants were
evaluated using MTT assay for viability and proliferation, immunocytochemistry
staining NOS for oxidative stress and TGFbeta1 for wound healing. It was found
that plant extracts reduced antioxidative damage and inhibited apoptosis. It
was observed that oxidative stress and apoptosis were increased in cancer
cells, but less effective in invasive cell lines. In vivo experiments showed
that wound healing was accelerated and that these rates were achieved with antioxidative
and antiapoptotic effects. It has been concluded that medicinal plants are
beneficial for treatment of difficult diseases in which patient quality of life
is very effective and they should be used as scientific-based medical
applications.

Kaynakça

  • Abaza MS, Orabi KY, Al-Quattan E, Al-Attiyah RJ. Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer. Cancer Cell Int. 2015 Apr 24;15:46. doi: 10.1186/s12935-015-0194-0.
  • Ahmadi A, Shadboorestan A. Oxidative stress and cancer; the role of hesperidin, a citrus natural bioflavonoid, as a cancer chemoprotective agent. Nutr Cancer. 2016;68(1):29-39. doi: 10.1080/01635581.2015.1078822.
  • Alamger, Mazhar U, Mushtaq MN, Khan HU, Maheen S, Malik MN, Ahmad T, Latif F, Tabassum N, Khan AQ, Ahsan H, Khan W, Javed I, Ali H. Evaluation of anti-inflammatory, analgesic and antipyretic activities of Thymus serphyllum Linn. in mice. Acta Pol Pharm. 2015 Jan-Feb;72(1):113-8.
  • Al-Menhali A, Al-Rumaihi A, Al-Mohammed H, Al-Mazrooey H, Al-Shamlan M, AlJassim M, Al-Korbi N, Eid AH. Thymus vulgaris (thyme) inhibits proliferation, adhesion, migration, and invasion of human colorectal cancer cells. J Med Food. 2015 Jan;18(1):54-9. doi: 10.1089/jmf.2013.3121.
  • Czompa A, Gyongyosi A, Szoke K, Bak I, Csepanyi E, Haines DD, Tosaki A, Lekli I. Effects of Momordica charantia (Bitter Melon) on Ischemic Diabetic Myocardium. Molecules. 2017 Mar 20;22(3). pii: E488. doi: 10.3390/molecules22030488.
  • Deliloglu-Gurhan I, Tuglu I, Vatansever HS, Ozdal-Kurt F, Ekren H, Taylan M, Sen BH. The effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatite. Saudi Med J. 2006; 27(3):305-11.
  • DeSantis CE, Ma J, Goding Sauer A, Newman LA, Jemal A. Breast cancer statistics, 2017, racial disparity in mortality by state. CA Cancer J Clin. 2017 Oct 3. doi: 10.3322/caac.21412.
  • Đorđević M, Mihailović M, Arambašić Jovanović J, Grdović N, Uskoković A, Tolić A, Sinadinović M, Rajić J, Mišić D, Šiler B, Poznanović G, Vidaković M, Dinić S. Centaurium erythraea methanol extract protects red blood cells from oxidative damage in streptozotocin-induced diabetic rats. J Ethnopharmacol. 2017 Apr 18;202:172-183. doi: 10.1016/j.jep.2017.03.016.
  • Dutta SW, Showalter SL, Showalter TN, Libby B, Trifiletti DM. Intraoperative radiation therapy for breast cancer patients: current perspectives. Breast Cancer (Dove Med Press). 2017 Apr 18;9:257-263. doi: 10.2147/BCTT.S112516.
  • Fatma G, Mouna BF, Mondher M, Ahmed L. In-vitro assessment of antioxidant and antimicrobial activities of methanol extracts and essential oil of Thymus hirtus sp. algeriensis. Lipids Health Dis. 2014 Jul 14;13:114. doi:10.1186/1476-511X-13-114.
  • Fistonić I, Situm M, Bulat V, Harapin M, Fistonić N, Verbanac D. Olive oil biophenols and women's health. Med Glas (Zenica). 2012 Feb;9(1):1-9.
  • Fogli S, Arena C, Carpi S, Polini B, Bertini S, Digiacomo M, Gado F, Saba A, Saccomanni G, Breschi MC, Nieri P, Manera C, Macchia M. Cytotoxic Activity of Oleocanthal Isolated from Virgin Olive Oil on Human Melanoma Cells. Nutr Cancer. 2016 Jul;68(5):873-7. doi: 10.1080/01635581.2016.1180407.
  • Gu Y, Wang J, Peng L. (-)-Oleocanthal exerts anti-melanoma activities and inhibits STAT3 signaling pathway. Oncol Rep. 2017 Jan;37(1):483-491. doi:10.3892/or.2016.5270.
  • Hu B, Sakakibara H, Takebayashi Y, Peters FS, Schumacher J, Eiblmeier M, Arab L, Kreuzwieser J, Polle A, Rennenberg H. Mistletoe infestation mediates alteration of the phytohormone profile and anti-oxidative metabolism in bark and wood of its host Pinus sylvestris. Tree Physiol. 2017 Feb 9:1-16. doi:10.1093/treephys/tpx006.
  • Hu X, Huang W, Fan M. Emerging therapies for breast cancer. J Hematol Oncol. 2017 Apr 28;10(1):98. doi: 10.1186/s13045-017-0466-3.
  • Kawaguchi T, Foster BA, Young J, Takabe K. Current Update of Patient-Derived Xenograft Model for Translational Breast Cancer Research. J Mammary Gland Biol Neoplasia. 2017 Apr 27. doi: 10.1007/s10911-017-9378-7.
  • Kienle GS, Glockmann A, Schink M, Kiene H. Viscum album L. extracts in breast and gynaecological cancers: a systematic review of clinical and preclinical research. J Exp Clin Cancer Res. 2009 Jun 11;28:79. doi: 10.1186/1756-9966-28-79.
  • Kuonen R, Weissenstein U, Urech K, Kunz M, Hostanska K, Estko M, Heusser P, Baumgartner S. Effects of Lipophilic Extract of Viscum album L. and Oleanolic Acid on Migratory Activity of NIH/3T3 Fibroblasts and on HaCat Keratinocytes. Evid Based Complement Alternat Med. 2013;2013:718105. doi: 10.1155/2013/718105.
  • Kurt O, Ozdal-Kurt F, Tuğlu MI, Akçora CM. The cytotoxic, neurotoxic, apoptotic and antiproliferative activities of extracts of some marine algae on the MCF-7 cell line. Biotech Histochem. 2014 Nov;89(8):568-76. doi: 10.3109/10520295.2014.917199.
  • Ligi D, Croce L, Mosti G, Raffetto JD, Mannello F. Chronic Venous Insufficiency: Transforming Growth Factor-β Isoforms and Soluble Endoglin Concentration in Different States of Wound Healing. Int J Mol Sci. 2017 Oct 21;18(10). pii: E2206. doi: 10.3390/ijms18102206.
  • Moore N, Hamza N, Berke B, Umar A. News from Tartary: an ethnopharmacological approach to drug and therapeutic discovery. Br J Clin Pharmacol. 2017Jan;83(1):33-37. doi: 10.1111/bcp.13042.
  • Narang N, Jiraungkoorskul W. Anticancer Activity of Key Lime, Citrus aurantifolia. Pharmacogn Rev. 2016 Jul-Dec;10(20):118-122. doi:10.4103/0973-7847.194043.
  • Parkinson L, Cicerale S. The Health Benefiting Mechanisms of Virgin Olive Oil Phenolic Compounds. Molecules. 2016 Dec 16;21(12). pii: E1734. doi:10.3390/molecules21121734.
  • Saad DY, Soliman MM, Baiomy AA, Yassin MH, El-Sawy HB. Effects of Karela (Bitter Melon; Momordica charantia) on genes of lipids and carbohydrates metabolism in experimental hypercholesterolemia: biochemical, molecular and histopathological study. BMC Complement Altern Med. 2017 Jun 17;17(1):319. doi: 10.1186/s12906-017-1833-x.
  • Sarandy MM, Novaes RD, Xavier AA, Vital CE, Leite JPV, Melo FCSA, Gonçalves RV. Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes. Biomed Res Int. 2017;2017:9538351. doi: 10.1155/2017/9538351.
  • Seca AM, Grigore A, Pinto DC, Silva AM. The genus Inula and their metabolites: from ethnopharmacological to medicinal uses. J Ethnopharmacol. 2014 Jun 11;154(2):286-310. doi:10.1016/j.jep.2014.04.010.
  • You HS, Fadriquela A, Sajo MEJ, Bajgai J, Ara J, Kim CS, Kim SK, Oh JR, Shim KY, Lim HK, Lee KJ. Wound Healing Effect of Slightly Acidic Electrolyzed Water on Cutaneous Wounds in Hairless Mice via Immune-Redox Modulation. Biol Pharm Bull. 2017;40(9):1423-1431. doi: 10.1248/bpb.b17-00219.
  • Zhang SR, Xu YS, Jin E, Zhu LC, Xia B, Chen XF, Li FZ, Ma SL. Capilliposide from Lysimachia capillipes inhibits AKT activation and restores gefitinib sensitivity in human non-small cell lung cancer cells with acquired gefitinib resistance. Acta Pharmacol Sin., 2017; 38(1):100-109. doi:10.1038/aps.2016.116.

The Effects of Medicinal Plants on Cancer Cell Lines and Efficacy of Experimental Animal Model

Yıl 2018, Cilt: 5 Sayı: 1, 49 - 59, 04.01.2018
https://doi.org/10.21448/ijsm.365065

Öz

The use of medicinal plants as an alternative treatment is a historical process and has been known for a long time so that classical treatment can be more effective in wound and cancer treatment. The purpose of this study was to investigate the effects of plant extracts used for therapeutic purposes in cancer cell lines in vitro wound model and in vivo experimental animal model in order to obtain this information. The plants, olive oil (Oleocanthal), mistletoe (Viscum album), Common Centaury (Centaurium erythraea), Momordica charantia, Inula viscosa, Citrus aurantium, Thyme oil (Thymus vulgaris) and algae (Jania longifurca), were used. MCF-7, MB-MDA-231, 67NR and 4T1 for breast, NB2a for neuron, L929 for fibroblast and normal somatic mesenchymal stem cell for comparison were selected for in vitro wound models. As an in vivo breast cancer model, female Balb/c mice were injected with 4T1 cells and skin wound healing in rats was investigated. The effects of medicinal plants were evaluated using MTT assay for viability and proliferation, immunocytochemistry staining NOS for oxidative stress and TGFbeta1 for wound healing. It was found that plant extracts reduced antioxidative damage and inhibited apoptosis. It was observed that oxidative stress and apoptosis were increased in cancer cells, but less effective in invasive cell lines. In vivo experiments showed that wound healing was accelerated and that these rates were achieved with antioxidative and antiapoptotic effects. It has been concluded that medicinal plants are beneficial for treatment of difficult diseases in which patient quality of life is very effective and they should be used as scientific-based medical applications.

Kaynakça

  • Abaza MS, Orabi KY, Al-Quattan E, Al-Attiyah RJ. Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer. Cancer Cell Int. 2015 Apr 24;15:46. doi: 10.1186/s12935-015-0194-0.
  • Ahmadi A, Shadboorestan A. Oxidative stress and cancer; the role of hesperidin, a citrus natural bioflavonoid, as a cancer chemoprotective agent. Nutr Cancer. 2016;68(1):29-39. doi: 10.1080/01635581.2015.1078822.
  • Alamger, Mazhar U, Mushtaq MN, Khan HU, Maheen S, Malik MN, Ahmad T, Latif F, Tabassum N, Khan AQ, Ahsan H, Khan W, Javed I, Ali H. Evaluation of anti-inflammatory, analgesic and antipyretic activities of Thymus serphyllum Linn. in mice. Acta Pol Pharm. 2015 Jan-Feb;72(1):113-8.
  • Al-Menhali A, Al-Rumaihi A, Al-Mohammed H, Al-Mazrooey H, Al-Shamlan M, AlJassim M, Al-Korbi N, Eid AH. Thymus vulgaris (thyme) inhibits proliferation, adhesion, migration, and invasion of human colorectal cancer cells. J Med Food. 2015 Jan;18(1):54-9. doi: 10.1089/jmf.2013.3121.
  • Czompa A, Gyongyosi A, Szoke K, Bak I, Csepanyi E, Haines DD, Tosaki A, Lekli I. Effects of Momordica charantia (Bitter Melon) on Ischemic Diabetic Myocardium. Molecules. 2017 Mar 20;22(3). pii: E488. doi: 10.3390/molecules22030488.
  • Deliloglu-Gurhan I, Tuglu I, Vatansever HS, Ozdal-Kurt F, Ekren H, Taylan M, Sen BH. The effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatite. Saudi Med J. 2006; 27(3):305-11.
  • DeSantis CE, Ma J, Goding Sauer A, Newman LA, Jemal A. Breast cancer statistics, 2017, racial disparity in mortality by state. CA Cancer J Clin. 2017 Oct 3. doi: 10.3322/caac.21412.
  • Đorđević M, Mihailović M, Arambašić Jovanović J, Grdović N, Uskoković A, Tolić A, Sinadinović M, Rajić J, Mišić D, Šiler B, Poznanović G, Vidaković M, Dinić S. Centaurium erythraea methanol extract protects red blood cells from oxidative damage in streptozotocin-induced diabetic rats. J Ethnopharmacol. 2017 Apr 18;202:172-183. doi: 10.1016/j.jep.2017.03.016.
  • Dutta SW, Showalter SL, Showalter TN, Libby B, Trifiletti DM. Intraoperative radiation therapy for breast cancer patients: current perspectives. Breast Cancer (Dove Med Press). 2017 Apr 18;9:257-263. doi: 10.2147/BCTT.S112516.
  • Fatma G, Mouna BF, Mondher M, Ahmed L. In-vitro assessment of antioxidant and antimicrobial activities of methanol extracts and essential oil of Thymus hirtus sp. algeriensis. Lipids Health Dis. 2014 Jul 14;13:114. doi:10.1186/1476-511X-13-114.
  • Fistonić I, Situm M, Bulat V, Harapin M, Fistonić N, Verbanac D. Olive oil biophenols and women's health. Med Glas (Zenica). 2012 Feb;9(1):1-9.
  • Fogli S, Arena C, Carpi S, Polini B, Bertini S, Digiacomo M, Gado F, Saba A, Saccomanni G, Breschi MC, Nieri P, Manera C, Macchia M. Cytotoxic Activity of Oleocanthal Isolated from Virgin Olive Oil on Human Melanoma Cells. Nutr Cancer. 2016 Jul;68(5):873-7. doi: 10.1080/01635581.2016.1180407.
  • Gu Y, Wang J, Peng L. (-)-Oleocanthal exerts anti-melanoma activities and inhibits STAT3 signaling pathway. Oncol Rep. 2017 Jan;37(1):483-491. doi:10.3892/or.2016.5270.
  • Hu B, Sakakibara H, Takebayashi Y, Peters FS, Schumacher J, Eiblmeier M, Arab L, Kreuzwieser J, Polle A, Rennenberg H. Mistletoe infestation mediates alteration of the phytohormone profile and anti-oxidative metabolism in bark and wood of its host Pinus sylvestris. Tree Physiol. 2017 Feb 9:1-16. doi:10.1093/treephys/tpx006.
  • Hu X, Huang W, Fan M. Emerging therapies for breast cancer. J Hematol Oncol. 2017 Apr 28;10(1):98. doi: 10.1186/s13045-017-0466-3.
  • Kawaguchi T, Foster BA, Young J, Takabe K. Current Update of Patient-Derived Xenograft Model for Translational Breast Cancer Research. J Mammary Gland Biol Neoplasia. 2017 Apr 27. doi: 10.1007/s10911-017-9378-7.
  • Kienle GS, Glockmann A, Schink M, Kiene H. Viscum album L. extracts in breast and gynaecological cancers: a systematic review of clinical and preclinical research. J Exp Clin Cancer Res. 2009 Jun 11;28:79. doi: 10.1186/1756-9966-28-79.
  • Kuonen R, Weissenstein U, Urech K, Kunz M, Hostanska K, Estko M, Heusser P, Baumgartner S. Effects of Lipophilic Extract of Viscum album L. and Oleanolic Acid on Migratory Activity of NIH/3T3 Fibroblasts and on HaCat Keratinocytes. Evid Based Complement Alternat Med. 2013;2013:718105. doi: 10.1155/2013/718105.
  • Kurt O, Ozdal-Kurt F, Tuğlu MI, Akçora CM. The cytotoxic, neurotoxic, apoptotic and antiproliferative activities of extracts of some marine algae on the MCF-7 cell line. Biotech Histochem. 2014 Nov;89(8):568-76. doi: 10.3109/10520295.2014.917199.
  • Ligi D, Croce L, Mosti G, Raffetto JD, Mannello F. Chronic Venous Insufficiency: Transforming Growth Factor-β Isoforms and Soluble Endoglin Concentration in Different States of Wound Healing. Int J Mol Sci. 2017 Oct 21;18(10). pii: E2206. doi: 10.3390/ijms18102206.
  • Moore N, Hamza N, Berke B, Umar A. News from Tartary: an ethnopharmacological approach to drug and therapeutic discovery. Br J Clin Pharmacol. 2017Jan;83(1):33-37. doi: 10.1111/bcp.13042.
  • Narang N, Jiraungkoorskul W. Anticancer Activity of Key Lime, Citrus aurantifolia. Pharmacogn Rev. 2016 Jul-Dec;10(20):118-122. doi:10.4103/0973-7847.194043.
  • Parkinson L, Cicerale S. The Health Benefiting Mechanisms of Virgin Olive Oil Phenolic Compounds. Molecules. 2016 Dec 16;21(12). pii: E1734. doi:10.3390/molecules21121734.
  • Saad DY, Soliman MM, Baiomy AA, Yassin MH, El-Sawy HB. Effects of Karela (Bitter Melon; Momordica charantia) on genes of lipids and carbohydrates metabolism in experimental hypercholesterolemia: biochemical, molecular and histopathological study. BMC Complement Altern Med. 2017 Jun 17;17(1):319. doi: 10.1186/s12906-017-1833-x.
  • Sarandy MM, Novaes RD, Xavier AA, Vital CE, Leite JPV, Melo FCSA, Gonçalves RV. Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes. Biomed Res Int. 2017;2017:9538351. doi: 10.1155/2017/9538351.
  • Seca AM, Grigore A, Pinto DC, Silva AM. The genus Inula and their metabolites: from ethnopharmacological to medicinal uses. J Ethnopharmacol. 2014 Jun 11;154(2):286-310. doi:10.1016/j.jep.2014.04.010.
  • You HS, Fadriquela A, Sajo MEJ, Bajgai J, Ara J, Kim CS, Kim SK, Oh JR, Shim KY, Lim HK, Lee KJ. Wound Healing Effect of Slightly Acidic Electrolyzed Water on Cutaneous Wounds in Hairless Mice via Immune-Redox Modulation. Biol Pharm Bull. 2017;40(9):1423-1431. doi: 10.1248/bpb.b17-00219.
  • Zhang SR, Xu YS, Jin E, Zhu LC, Xia B, Chen XF, Li FZ, Ma SL. Capilliposide from Lysimachia capillipes inhibits AKT activation and restores gefitinib sensitivity in human non-small cell lung cancer cells with acquired gefitinib resistance. Acta Pharmacol Sin., 2017; 38(1):100-109. doi:10.1038/aps.2016.116.
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Makaleler
Yazarlar

Mehmet İbrahim Tuğlu

İşıl Aydemir Bu kişi benim

Pınar Kılıçarslan Sönmez Bu kişi benim

Tahir Buran

Mesut Mete

Yayımlanma Tarihi 4 Ocak 2018
Gönderilme Tarihi 23 Ekim 2017
Yayımlandığı Sayı Yıl 2018 Cilt: 5 Sayı: 1

Kaynak Göster

APA Tuğlu, M. İ., Aydemir, İ., Kılıçarslan Sönmez, P., Buran, T., vd. (2018). The Effects of Medicinal Plants on Cancer Cell Lines and Efficacy of Experimental Animal Model. International Journal of Secondary Metabolite, 5(1), 49-59. https://doi.org/10.21448/ijsm.365065
International Journal of Secondary Metabolite
e-ISSN: 2148-6905