Öz
Aim. DOG1 is a calcium-dependent chlorid channel, that shows specific expression in Gastrointestinal stromal tumors (GIST) can be used in differential diagnosis of these tumors with other soft tissue sarcomas. In several studies, DOG1 expression has been reported in varying proportions in Non-GIST mesenchymal tumors. In this study, we aimed to find the DOG1 expression profile in Nodular fasciit, Neurofibroma, Desmoid tumors and Dermatofibrosarcoma protuberans. Method. Nodular fasciit (n=8), Neurofibroma (n=8), Desmoid tumors (n=8) and Dermatofibrosarcoma protuberans (n=8) cases, diagnosed in Ankara University, Faculty of Medicine, Department of Pathology between 2005-2012, were included in this study. Results. Focal and pale DOG1 reactivity was seen only in one case of Nodular fasciit (1/8) whereas the remainig cases were negative with DOG1. Conclusion. DOG1 has no diagnostic value in benign and locally aggressive fibroblastic/myofibroblastic neoplasms and neurofibromas.
Anahtar Kelimeler
DOG1 neurofibroma nodular fasciitis desmoid tumor dermatofibrosarcoma protuberans
Kaynakça
- Miettinen M. Gastrointestinal stromal tumors: Pathology and prognosis at different sites. Semin Diagn Pathol 2006; 23: 70-83. Lasota J. KIT and PDGFRA mutations in gastrointestinal stromal tumors (GISTs). Semin Diagn Pathol 2006; 23: 91-102.
- Joensuu H. Risk stratification of patients diagnosed with gastrointestinal stromal tumor. Hum Pathol 2008; 39: 1411-9.
- Katoh M. FLJ10261 gene, located within the CCND1- EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products. Int. J. Oncol 2003; 22; 1375
- West RB. The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGFRA mutation status. Am. J of Pathol 2004; 165: 107-13.
- Lopes LF. DOG1 for the diagnosis of gastrointestinal stromal tumor (GIST): Comparison between 2 different antibodies. Appl Immunohistochem Mol Morphol 2010; 18: 333-7.
- Liegl B. Monoclonal antibody DOG1.1 shows higher sensitivity than KIT in the diagnosis of gastrointestinal stromal tumors including unusual subtypes. Am. J. Surg. Pathol 2009; 33; 437-46.
- Hemminger J. Discovered on gastrointestinal stromal tumours 1 (DOG1) expression in non-gastrointestinal stromal tumour (GIST) neoplasms. Histopathology 2012; 61; 170-7.
- Wong NA. Specificity of DOG1 (K9 clone) and protein kinase theta (clone 27) as immunohistochemical markers of gastrointestinal stromal tumor. Histopathology 2010; 57: 250-8.
- Espinosa I. A novel monoclonal antibody against DOG1 is a sensitive and specific marker for gastrointestinal stromal tumors. Am J Surg Pathol 2008; 32: 210
Fibroblastik/myofibroblastik benign ve lokal agresif neoplaziler ve nörofibromalarda DOG1 ekspresyon profili
Öz
Özet
Amaç. Gastrointestinal stromal tümörlerde (GİST) spesifik olarak ekspresyon gösteren ve kalsiyum bağımlı klorid kanalı olarak bilinen DOG1, gastrointestinal stromal tümörlerin diğer sarkomlardan ayrımında kullanılan tanısal bir belirteçtir. Çeşitli araştırmalarda Gastrointestinal stromal tümor dışı mezenşimal tümörlerde değişen oranda DOG1 ekspresyonu bildirilmiştir. Bu çalışmada Nodüler fasiit, Nörofibroma, Desmoid tümör ve Dermatofibrosarkoma Protuberans olgularından oluşan bir vaka serisinde DOG1 ekspresyonu araştırılmıştır. Yöntem. Bu çalışmaya, Ankara Üniversitesi Tıp Fakültesi Tıbbi Patoloji Anabilim Dalı’nda 2005-2012 yılları arasında tanı almış Nodüler Fasiit (n=8), Desmoid tümör (n=8), Dermatofibrosarkoma Protuberans (n=8) ve Nörofibroma (n=8) vakaları dahil edilmiştir. Parafin bloklardan elde edilen kesitlerde otomatik immün boyama cihazı ile DOG1 çalışılmıştır. Bulgular. Yalnızca bir Nodüler fasiit olgusunda (1/8) fokal soluk sitoplazmik boyanma izlenirken, diğer olgularda DOG1 ekspresyonu saptanmamıştır. Sonuç. Fibroblastik /myofibroblastik benign ve lokal agresif neoplaziler ve nörofibromalarda DOG1’in tanısal değeri yoktur.
Anahtar sözcükler: DOG1, nörofibroma, nodüler fasiit, desmoid tümör, dermatofibrosarkoma protuberans
Abstract
Aim. DOG1 is a calcium-dependent chlorid channel, that shows specific expression in Gastrointestinal stromal tumors (GIST) can be used in differential diagnosis of these tumors with other soft tissue sarcomas. In several studies, DOG1 expression has been reported in varying proportions in Non-GIST mesenchymal tumors. In this study, we aimed to find the DOG1 expression profile in Nodular fasciit, Neurofibroma, Desmoid tumors and Dermatofibrosarcoma protuberans. Method. Nodular fasciit (n=8), Neurofibroma (n=8), Desmoid tumors (n=8) and Dermatofibrosarcoma protuberans (n=8) cases, diagnosed in Ankara University, Faculty of Medicine, Department of Pathology between 2005-2012, were included in this study. Results. Focal and pale DOG1 reactivity was seen only in one case of Nodular fasciit (1/8) whereas the remainig cases were negative with DOG1. Conclusion. DOG1 has no diagnostic value in benign and locally aggressive fibroblastic/myofibroblastic neoplasms and neurofibromas.
Keywords: DOG1, neurofibroma, nodular fasciitis, desmoid tumor, dermatofibrosarcoma protuberans
Anahtar Kelimeler
DOG1 nörofibroma nodüler fasiit desmoid tümör dermatofibrosarkoma protuberans
Kaynakça
- Miettinen M. Gastrointestinal stromal tumors: Pathology and prognosis at different sites. Semin Diagn Pathol 2006; 23: 70-83. Lasota J. KIT and PDGFRA mutations in gastrointestinal stromal tumors (GISTs). Semin Diagn Pathol 2006; 23: 91-102.
- Joensuu H. Risk stratification of patients diagnosed with gastrointestinal stromal tumor. Hum Pathol 2008; 39: 1411-9.
- Katoh M. FLJ10261 gene, located within the CCND1- EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products. Int. J. Oncol 2003; 22; 1375
- West RB. The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGFRA mutation status. Am. J of Pathol 2004; 165: 107-13.
- Lopes LF. DOG1 for the diagnosis of gastrointestinal stromal tumor (GIST): Comparison between 2 different antibodies. Appl Immunohistochem Mol Morphol 2010; 18: 333-7.
- Liegl B. Monoclonal antibody DOG1.1 shows higher sensitivity than KIT in the diagnosis of gastrointestinal stromal tumors including unusual subtypes. Am. J. Surg. Pathol 2009; 33; 437-46.
- Hemminger J. Discovered on gastrointestinal stromal tumours 1 (DOG1) expression in non-gastrointestinal stromal tumour (GIST) neoplasms. Histopathology 2012; 61; 170-7.
- Wong NA. Specificity of DOG1 (K9 clone) and protein kinase theta (clone 27) as immunohistochemical markers of gastrointestinal stromal tumor. Histopathology 2010; 57: 250-8.
- Espinosa I. A novel monoclonal antibody against DOG1 is a sensitive and specific marker for gastrointestinal stromal tumors. Am J Surg Pathol 2008; 32: 210
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Cerrahi Tıp Bilimleri Araştırma Yazıları |
| Yazarlar | |
| Yayımlanma Tarihi | 8 Şubat 2014 |
| Yayımlandığı Sayı | Yıl 2014Cilt: 36 Sayı: 2 |