Araştırma Makalesi
Morphological and Molecular Identification of Cytotoxic Fungus Species; Hebeloma crustuliniforme and Hebeloma sinapizans
Öz
Taxonomic positions and phylogenetic relations of Hebeloma species have not yet been figured out molecularly in Turkey. Most species of the genus are mainly poisonous and a few of which are edible. Hebeloma crustuliniforme and H. sinapizans are two important species that contain deadly toxins so only morphological characters may not be enough for reliable identification. The DNA sequence of the nuclear ribosomal Internal Transcribed spacer (nrITS) region is used in addition to morphological characters in the present study. Phylogenetic analysis is conducted on the sequence dataset by using the Maximum Likelihood (ML) principle to see the taxonomic placement of two poisonous species. Studying morphological traits and molecular markers together is very useful to distinguish these two poisonous species from close relatives. The first sequence data of these species were added to the literature on behalf of our country with this study.
Anahtar Kelimeler
Kaynakça
- Beker, H.J., Eberhardt, U., Vesterholt, J., 2016. Fungi Europaei Vol. 14, Hebeloma (Fr.) P. Kumm. Edizioni Tecnografica.
- Benjamin, D.R., 1995. Mushrooms: poisons and panaceas—a handbook for naturalists, mycologists, and physicians. New York: W.H. Freeman. 416 p.
- Bresinsky, A., Besl, H., 1990. A colour atlas of poisonous fungi; a handbook for pharmacists, doctors, and biologists. London, UK: Wolfe. 251 p.
- Carrasco-Hernández, V., Pérez-Moreno, J., Quintero-Lizaola, R., Espinosa-Solares, T., Lorenzana-Fernández, A., Espinosa-Hernández, V., 2015. Edible species of the fungal genus Hebeloma and two neotropical pines. Pakistan Journal of Botany, 47:319-326.
- De Bernardi, M. Fronza, M.G., Gianottia, M.P., Mellerioa, G., Vidaria, G., Vita-Finzia, P., 1983. Fungal Metabolites Xiii: New Cytotoxic Triterpene from Hebeloma Species (Basidiomycetes). Tetrahedron Letters, 24:1635-1638.
- Dizkirici, A., Acar, A., Kalmer, A., Uzun, Y., 2019. Morphological and molecular characterization of Hebeloma subtortum (Hymenogastraceae), a new record macrofungus from Bingöl province, Turkey. Kastamonu Univ., Journal of Forestry Faculty. 19(1): 1-10.
- Eberhardt, U., Beker, H.J., Schütz, N., Pedersen, O.S., Sysouphanthong, P., Læssøe, T., 2020. Adventurous cuisine in Laos: Hebeloma parvisporum, a new species in Hebeloma section Porphyrospora. Mycologia, DOI: 10.1080/00275514.2019.1680220
- Grilli, E., Beker, H., Eberhardt, U., Schütz, N., Leonardi, M., Vizzini, A., 2016. Unexpected species diversity and contrasting evolutionary hypotheses in Hebeloma (Agaricales) sections Sinapizantia and Velutipes in Europe. Mycological Progress, 15: 5.
- Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W., Gascuel, O., 2010. New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Systematic Biology, 59(3):307-321.
- Index fungorum, CABI Bioscience Databases, http://www.indexfungorum.org (last accession: 03.11.2021).
- McDougall, W.B. 1925. A Handbook of Mushrooms, Boston and New York, Houghton Mifflin Co., p. 151. Montoya, A., Kong, A., Estrada-Torres, A., Cifuentes, J., Caballero, J., 2004. Useful wild fungi of La Malinche Nacional Park, Mexico. Fungal Diversity, 17:115-143.
- Montoya, A., Hernández, N., Mapes, C., Kong, A., Estrada-Torres, A., 2008. The collection and sale of wild mushrooms in a community of Tlaxcala, Mexico. Economic Botany, 62:413-424.
- O'Reilly, P., 2016. Fascinated by Fungi, First Nature Press, 2nd edition, Wales, UK. p. 450.
- Pamela, N., 1967. Poisonous Plants and Fungi in colour. Blandford Press & Pharmacological, Society of Great Britain.
- Pérez-Moreno, J., Martínez-Reyes, M., Yescas-Pérez, A., Delgado-Alvarado, A., Xoconostle-Cázares, B., 2008. Wild mushroom markets in central Mexico and a case study at Ozumba. Economic Botany, 62:425-436.
- Roger, P., 2010. Mushrooms and Other Fungi of North America. Buffalo, NY: Firefly Books. p. 206. ISBN 978-155407-651-2.
- Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A., Chen, W., 2012. Fungal Barcoding Consortium Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. PNAS, 109: 6241-6246.
- Shao, H.J., Qing, C., Wang, F., Zhang, Y.L., Luo, D.Q., Liu, J.K., 2005. A New Cytotoxic Lanostane Triterpenoid from the Basidiomycete Hebeloma versipelle. The Journal of Antibiotics, 58: 828-831.
- Tamura, K., Stecher, G., Peterson, D., Filipski, A., M., Kumar, S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30(12): 2725-2729.
- Thompson, J.D., Higgins, D.G., Gibson, T.J., 1994. Clustal W improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680.
- Vesterholt, J., 2005. The Genus Hebeloma. [Fungi of Northern Europe Volume 3] Danish Mycological Society, Copenhagen.
- Vesterholt, J., Eberhardt, U., Beker, H.J., 2014. Epitypification of Hebeloma crustuliniforme. Mycological Progress, 13: 553-562.
Yıl 2022,
Cilt: 5 Sayı: 1, 10 - 17, 30.06.2022
Öz
Kaynakça
- Beker, H.J., Eberhardt, U., Vesterholt, J., 2016. Fungi Europaei Vol. 14, Hebeloma (Fr.) P. Kumm. Edizioni Tecnografica.
- Benjamin, D.R., 1995. Mushrooms: poisons and panaceas—a handbook for naturalists, mycologists, and physicians. New York: W.H. Freeman. 416 p.
- Bresinsky, A., Besl, H., 1990. A colour atlas of poisonous fungi; a handbook for pharmacists, doctors, and biologists. London, UK: Wolfe. 251 p.
- Carrasco-Hernández, V., Pérez-Moreno, J., Quintero-Lizaola, R., Espinosa-Solares, T., Lorenzana-Fernández, A., Espinosa-Hernández, V., 2015. Edible species of the fungal genus Hebeloma and two neotropical pines. Pakistan Journal of Botany, 47:319-326.
- De Bernardi, M. Fronza, M.G., Gianottia, M.P., Mellerioa, G., Vidaria, G., Vita-Finzia, P., 1983. Fungal Metabolites Xiii: New Cytotoxic Triterpene from Hebeloma Species (Basidiomycetes). Tetrahedron Letters, 24:1635-1638.
- Dizkirici, A., Acar, A., Kalmer, A., Uzun, Y., 2019. Morphological and molecular characterization of Hebeloma subtortum (Hymenogastraceae), a new record macrofungus from Bingöl province, Turkey. Kastamonu Univ., Journal of Forestry Faculty. 19(1): 1-10.
- Eberhardt, U., Beker, H.J., Schütz, N., Pedersen, O.S., Sysouphanthong, P., Læssøe, T., 2020. Adventurous cuisine in Laos: Hebeloma parvisporum, a new species in Hebeloma section Porphyrospora. Mycologia, DOI: 10.1080/00275514.2019.1680220
- Grilli, E., Beker, H., Eberhardt, U., Schütz, N., Leonardi, M., Vizzini, A., 2016. Unexpected species diversity and contrasting evolutionary hypotheses in Hebeloma (Agaricales) sections Sinapizantia and Velutipes in Europe. Mycological Progress, 15: 5.
- Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W., Gascuel, O., 2010. New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Systematic Biology, 59(3):307-321.
- Index fungorum, CABI Bioscience Databases, http://www.indexfungorum.org (last accession: 03.11.2021).
- McDougall, W.B. 1925. A Handbook of Mushrooms, Boston and New York, Houghton Mifflin Co., p. 151. Montoya, A., Kong, A., Estrada-Torres, A., Cifuentes, J., Caballero, J., 2004. Useful wild fungi of La Malinche Nacional Park, Mexico. Fungal Diversity, 17:115-143.
- Montoya, A., Hernández, N., Mapes, C., Kong, A., Estrada-Torres, A., 2008. The collection and sale of wild mushrooms in a community of Tlaxcala, Mexico. Economic Botany, 62:413-424.
- O'Reilly, P., 2016. Fascinated by Fungi, First Nature Press, 2nd edition, Wales, UK. p. 450.
- Pamela, N., 1967. Poisonous Plants and Fungi in colour. Blandford Press & Pharmacological, Society of Great Britain.
- Pérez-Moreno, J., Martínez-Reyes, M., Yescas-Pérez, A., Delgado-Alvarado, A., Xoconostle-Cázares, B., 2008. Wild mushroom markets in central Mexico and a case study at Ozumba. Economic Botany, 62:425-436.
- Roger, P., 2010. Mushrooms and Other Fungi of North America. Buffalo, NY: Firefly Books. p. 206. ISBN 978-155407-651-2.
- Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A., Chen, W., 2012. Fungal Barcoding Consortium Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. PNAS, 109: 6241-6246.
- Shao, H.J., Qing, C., Wang, F., Zhang, Y.L., Luo, D.Q., Liu, J.K., 2005. A New Cytotoxic Lanostane Triterpenoid from the Basidiomycete Hebeloma versipelle. The Journal of Antibiotics, 58: 828-831.
- Tamura, K., Stecher, G., Peterson, D., Filipski, A., M., Kumar, S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30(12): 2725-2729.
- Thompson, J.D., Higgins, D.G., Gibson, T.J., 1994. Clustal W improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680.
- Vesterholt, J., 2005. The Genus Hebeloma. [Fungi of Northern Europe Volume 3] Danish Mycological Society, Copenhagen.
- Vesterholt, J., Eberhardt, U., Beker, H.J., 2014. Epitypification of Hebeloma crustuliniforme. Mycological Progress, 13: 553-562.
Toplam 22 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Ziraat Mühendisliği |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Haziran 2022 |
| Gönderilme Tarihi | 3 Aralık 2021 |
| Kabul Tarihi | 23 Ocak 2022 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 5 Sayı: 1 |
