Araştırma Makalesi
Yıl 2018,
Cilt: 12 Sayı: 3, 37 - 41, 26.12.2018
Öz
In our study we aimed to demonstrate Y microdeletions with using sY254 sequence tagged sites (STS) marker in primary infertile male patients by using Polymerase Chain Reaction (PCR) method and determine the correlation between cyctogenetic evaluation of patients. Twenty five male patients referred from Manisa and it’s regions, who were diagnosed with primary infertility in the urology department of Manisa Celal Bayar University were included in our study. Thirteen individuals had azoospermia, twelve individuals had oligozoospermia. Ethics committee approval of our patients were recieved from Manisa Celal Bayar University. Blood material was taken under sterile conditions from individuals. Total DNA was isolated from peripheral blood. DNA purity was checked. Target gene regions of total DNA were amplified with PCR method by using oligonucleotide primers. sY14 STS marker used as internal control and pure water used as negative control. Cytogenetic study was made with lymphocyte cultures, human metaphase chromosomes were analysed with light microscopy. According to our analysis results no microdeletion was determined and all of the patients had normal karyotype (46, XY)
Anahtar Kelimeler
Kaynakça
- [1] Mojtabanezhad Shariatpanahi A., Ahmadnia H., Torkamanzehi A., Mansouri Torshizi M., Kerachian M.A. 2018. Multiplex-Polymerase Chain Reaction for Detecting Microdeletions in The Azoospermia Factor Region of Y Chromosome in Iranian Couples with Non-Obstructive Infertility and Recurrent Pregnancy Loss. Int.J. Fertil Steril 11(4): 253-257. doi: 10.22074/ijfs.2018.5162 [2] Akınsal E.C., Baydilli N., Dündar M., Ekmekçioğlu O. 2018. The frequencies of Y chromosome microdeletions in infertile males. Turk J. Urol. DOI: 10.5152/tud.2018.73669. [3] Nailwal M. And Chauhan J.B. 2017. Azoospermia Factor C Subregion of the Y Chromosome. J Hum Reprod Sci. Oct-Dec; 10(4): 256–260.doi: 10.4103/jhrs.JHRS_16_17. [4] Kim S.Y., Kim H.J., Lee B.Y., Park S.Y., Lee H.S., Seo J.T. 2017. Y Chromosome Microdeletions in Infertile Men with Non-obstructive Azoospermia and Severe Oligozoospermia. J Reprod Infertil. 18(3):307-315. [5] Yun Zheng H., Li Y., Jin Shen F. 2014. A novel multiplex PCR improves detection of AZFc Y chromosome microdeletions. J Assist Reprod Genet 31:613–620 DOI 10.1007/s10815-014-0204-5 [6] Ambulkar P., Chuadhary A., Waghmare J., Tarnekar A., Pal A. 2015. Prevalence of Y chromosome microdeletions in idiopathic azoospermia cases in central Indian men. Journal of Clinical and Diagnostic Research Sep, Vol-9(9): GC01-GC04 [7] Pryor J.L., First M.K., Muallem A., Van Bergen A.H., Nolten W.E., Meisner L. and Roberts K.P. 1997. Microdeletions in the Y chromosome of infertile men. The New England Journal of Medicine February 20, Volume 336, Number 8; 534-539. [8] Atia T., Abbas M., Ahmed A.F. 2015. Azoospermia factor microdeletion in infertile men with idiopathic severe oligozoospermia or non-obstructive azoospermia. African Journal of Urology (2015) 21, 246–253. [9] Dada R., Gupta N.P. and Kucheria K. 2004. Yq microdeletions azoospermia factor candidate genes and spermatogenic arrest. Journal of Biomolecular Techniques, Volume 15, Issue 3, September. [10] Elfateh F., Rulin D., Xin Y., Linlin L., Haibo Z., Liu R.Z. 2014. Prevalence and patterns of Y chromosome microdeletion in infertile men with azoospermia and oligzoospermia in Northeast China Iran J Reprod Med Vol. 12. No. 6. pp: 383-388, June. [11] Behulova R., Varga I., Strhakova L., Bozikova A., Gabrikova D., Boronova I., Repiska V. 2011. Incidence of microdeletions in the AZF region of the Y chromosome in Slovak patients with azoospermia. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub.; 155:XX. DOI 10.5507/bp.2011.006. [12] Li-hua L.U., Fei-xue H.U., Song G.E. 2013. Investigation on Azoospermia Factor (AZF) Microdeletion and Sex-determining Region Y (SRY) of the Y Chromosome in Male Infertility. Journal of Reproduction & Contraception Jun.; 24(2):88-94 doi: 10.7669/j.issn.1001-7844.2013.02.0088. [13] Pan Y., Zhang H., Xi Q., Zhang H., Wang R., Li L. and Liu R. 2018. Molecular microdeletion analysis of infertile men with karyotypic Y chromosome abnormalities. Journal of International Medical Research Vol. 46(1) 307–315. DOI: 10.1177/0300060517719394. [14] Masoudi R., Asadi L.M., Khorasani S. 2016. Partial and complete microdeletions of Y chromosome in infertile males from South of Iran. Molecular Biology Research Communications; 5(4):247-255. [15] Rajneesh C.P., Kumar C.S., Manimaran A., Khan A.A., Dey J.,Yengkhom O., Vasudev G., Vijaya P.P., Sugumaran J. and Manivannan S. 2010. Sequence Tagged Site (STS) Analysis of Y-Chromosome Micro Deletions in Environmental Tobacco Smokers [ETS] in Tamilnadu, India. Advances in Biological Research 4 (6): 296-300. [16] Saliminejad K. and Khorram Khorshid H.R. 2012. Methodological errors in screening of Yq microdeletion in Iranian azoospermic men. Indian J Med Res 135, January, pp 137-139.
Yıl 2018,
Cilt: 12 Sayı: 3, 37 - 41, 26.12.2018
Öz
Kaynakça
- [1] Mojtabanezhad Shariatpanahi A., Ahmadnia H., Torkamanzehi A., Mansouri Torshizi M., Kerachian M.A. 2018. Multiplex-Polymerase Chain Reaction for Detecting Microdeletions in The Azoospermia Factor Region of Y Chromosome in Iranian Couples with Non-Obstructive Infertility and Recurrent Pregnancy Loss. Int.J. Fertil Steril 11(4): 253-257. doi: 10.22074/ijfs.2018.5162 [2] Akınsal E.C., Baydilli N., Dündar M., Ekmekçioğlu O. 2018. The frequencies of Y chromosome microdeletions in infertile males. Turk J. Urol. DOI: 10.5152/tud.2018.73669. [3] Nailwal M. And Chauhan J.B. 2017. Azoospermia Factor C Subregion of the Y Chromosome. J Hum Reprod Sci. Oct-Dec; 10(4): 256–260.doi: 10.4103/jhrs.JHRS_16_17. [4] Kim S.Y., Kim H.J., Lee B.Y., Park S.Y., Lee H.S., Seo J.T. 2017. Y Chromosome Microdeletions in Infertile Men with Non-obstructive Azoospermia and Severe Oligozoospermia. J Reprod Infertil. 18(3):307-315. [5] Yun Zheng H., Li Y., Jin Shen F. 2014. A novel multiplex PCR improves detection of AZFc Y chromosome microdeletions. J Assist Reprod Genet 31:613–620 DOI 10.1007/s10815-014-0204-5 [6] Ambulkar P., Chuadhary A., Waghmare J., Tarnekar A., Pal A. 2015. Prevalence of Y chromosome microdeletions in idiopathic azoospermia cases in central Indian men. Journal of Clinical and Diagnostic Research Sep, Vol-9(9): GC01-GC04 [7] Pryor J.L., First M.K., Muallem A., Van Bergen A.H., Nolten W.E., Meisner L. and Roberts K.P. 1997. Microdeletions in the Y chromosome of infertile men. The New England Journal of Medicine February 20, Volume 336, Number 8; 534-539. [8] Atia T., Abbas M., Ahmed A.F. 2015. Azoospermia factor microdeletion in infertile men with idiopathic severe oligozoospermia or non-obstructive azoospermia. African Journal of Urology (2015) 21, 246–253. [9] Dada R., Gupta N.P. and Kucheria K. 2004. Yq microdeletions azoospermia factor candidate genes and spermatogenic arrest. Journal of Biomolecular Techniques, Volume 15, Issue 3, September. [10] Elfateh F., Rulin D., Xin Y., Linlin L., Haibo Z., Liu R.Z. 2014. Prevalence and patterns of Y chromosome microdeletion in infertile men with azoospermia and oligzoospermia in Northeast China Iran J Reprod Med Vol. 12. No. 6. pp: 383-388, June. [11] Behulova R., Varga I., Strhakova L., Bozikova A., Gabrikova D., Boronova I., Repiska V. 2011. Incidence of microdeletions in the AZF region of the Y chromosome in Slovak patients with azoospermia. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub.; 155:XX. DOI 10.5507/bp.2011.006. [12] Li-hua L.U., Fei-xue H.U., Song G.E. 2013. Investigation on Azoospermia Factor (AZF) Microdeletion and Sex-determining Region Y (SRY) of the Y Chromosome in Male Infertility. Journal of Reproduction & Contraception Jun.; 24(2):88-94 doi: 10.7669/j.issn.1001-7844.2013.02.0088. [13] Pan Y., Zhang H., Xi Q., Zhang H., Wang R., Li L. and Liu R. 2018. Molecular microdeletion analysis of infertile men with karyotypic Y chromosome abnormalities. Journal of International Medical Research Vol. 46(1) 307–315. DOI: 10.1177/0300060517719394. [14] Masoudi R., Asadi L.M., Khorasani S. 2016. Partial and complete microdeletions of Y chromosome in infertile males from South of Iran. Molecular Biology Research Communications; 5(4):247-255. [15] Rajneesh C.P., Kumar C.S., Manimaran A., Khan A.A., Dey J.,Yengkhom O., Vasudev G., Vijaya P.P., Sugumaran J. and Manivannan S. 2010. Sequence Tagged Site (STS) Analysis of Y-Chromosome Micro Deletions in Environmental Tobacco Smokers [ETS] in Tamilnadu, India. Advances in Biological Research 4 (6): 296-300. [16] Saliminejad K. and Khorram Khorshid H.R. 2012. Methodological errors in screening of Yq microdeletion in Iranian azoospermic men. Indian J Med Res 135, January, pp 137-139.
Toplam 1 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 26 Aralık 2018 |
| Yayımlandığı Sayı | Yıl 2018 Cilt: 12 Sayı: 3 |
