Öz
Kaynakça
- 1. Ingels C, Gunst J, Van den Berghe G.Endocrine and Metabolic Alterations in Sepsis and Implications for Treatment. Crit Care Clin 2018;34:81-96.
- 2. SepNet Critical Care Trials Group. Incidence of severe sepsis and septic shock in German intensive care units: the prospective, multicentre INSEP study. Intensive Care Med 2016;42:1980-9.
- 3. Jaber S, Paugam C, Futier E, Lefrant JY, et al. BICAR-ICU Study Group. Sodium bicarbonate therapy for patients with severe metabolic acidaemia in the intensive care unit (BICAR-ICU): a multicentre, open-label, randomised controlled, phase 3 trial. Lancet 2018 7;392:31-40.
- 4. Reitsema VA, Star BS, de Jager VD, van Meurs M, et al. Metabolic Resuscitation Strategies to Prevent Organ Dysfunction in Sepsis. Antioxid Redox Signal 2019 10;31:134-52.
- 5. Vishnupriya K, Falade O, Workneh A, Chandolu S, et al. Does sepsis treatment differ between primary and overflow intensive care units? J Hosp Med 2012;7:600-5.
- 6. Polat G, Ugan RA, Cadirci E, Halici Z. Sepsis and Septic Shock: Current Treatment Strategies and New Approaches. Eurasian J Med 2017;49:53-8.
- 7. Thompson KD, Welykyj S, Massa MC. Antibacterial activity of lidocaine in combination with a bicarbonate buffer. J Dermatol Surg Oncol 1993;19:216-20.
- 8. Farha MA, French S, Stokes JM, Brown ED. Bicarbonate Alters Bacterial Susceptibility to Antibiotics by Targeting the Proton Motive Force. ACS Infect Dis 2018 9;4:382-90.
- 9. Begec Z, Gulhas N, Toprak HI, Yetkin G, et al. Comparison of the antibacterial activity of lidocaine 1% versus alkalinized lidocaine in vitro. Curr Ther Res Clin Exp 2007;68:242-8.
Bicarbonate may alters bacterial susceptibility to antibiotics by targeting Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus
Öz
Backgrounds/aims: Acute acidemia is a common clinical
condition in critical diseases. Acidemia is associated with poor prognosis in
case of persistence. In the case of metabolic acidosis, it is beneficial to
increase the pH by administering sodium bicarbonate (NaHCO3) since cell
functions are impaired. The aim of this study was to investigate the
antibacterial efficacy of NaHCO3 used in metabolic acidosis, especially in
critically ill patients in intensive care units, and to reveal its contribution
to antimicrobial therapy for possible concomitant sepsis.
Method: S.aureus ATCC 29213, P. aeruginosa ATCC
27853 and E. coli ATCC 25922 strains were seeded into liquid Müller Hinton
medium (Oxoid, UK) and the in-vitro effect of Group C (Control - 1 mL sterile
saline) and Group B (Sodium Bicarbonate - NaHCO3) on these bacteria following
24 hours of incubation at 37 degreesC was investigated. Following
the use of Epoch spectrophotometer (BioTek Inst. Inc. Vermont, USA) for the 0.
and 24. hours, the growth in wells was analyzed in CFU / mL and log10 CFU/mL by
comparison with the standard curve.
Results:From the start to the 24. hour, there was
a significant decrease in bacterial colony numbers of S.aureus ATCC 29213, P.
aeruginosa ATCC 27853 and E. coli ATCC 25922 strains in Group B when compared
to the control group (p <0.01).
The intra-group antibacterial efficacy comparison revealed a significant
decrease in bacterial colony numbers in Group B between 0-24 hours (p<0.01). There was a significant
increase in all bacterial colony numbers in the control group (p = 0.04).
Conclusion: In our study, NaHCO3 was found to show strong
antibacterial efficacy against P. aeruginosa, E. coli and S. aureus. Taking
these results into consideration, it should be kept in mind that the use of
NaHCO3 in the treatment of severe metabolic acidosis especially seen in septic
patients in intensive care units will also contribute to sepsis treatment
because of its antibacterial effect potential. We believe that the results of
this study, if supported by clinical studies, may contribute to the improvement
of treatment efficacy and lower treatment costs in critical patients in the
intensive care unit in the case of metabolic acidosis and sepsis.
Anahtar Kelimeler
Antibacterial sodium bicarbonate Pseudomonas Aeruginosa Staphylococcus aureus Escherichia Coli
Kaynakça
- 1. Ingels C, Gunst J, Van den Berghe G.Endocrine and Metabolic Alterations in Sepsis and Implications for Treatment. Crit Care Clin 2018;34:81-96.
- 2. SepNet Critical Care Trials Group. Incidence of severe sepsis and septic shock in German intensive care units: the prospective, multicentre INSEP study. Intensive Care Med 2016;42:1980-9.
- 3. Jaber S, Paugam C, Futier E, Lefrant JY, et al. BICAR-ICU Study Group. Sodium bicarbonate therapy for patients with severe metabolic acidaemia in the intensive care unit (BICAR-ICU): a multicentre, open-label, randomised controlled, phase 3 trial. Lancet 2018 7;392:31-40.
- 4. Reitsema VA, Star BS, de Jager VD, van Meurs M, et al. Metabolic Resuscitation Strategies to Prevent Organ Dysfunction in Sepsis. Antioxid Redox Signal 2019 10;31:134-52.
- 5. Vishnupriya K, Falade O, Workneh A, Chandolu S, et al. Does sepsis treatment differ between primary and overflow intensive care units? J Hosp Med 2012;7:600-5.
- 6. Polat G, Ugan RA, Cadirci E, Halici Z. Sepsis and Septic Shock: Current Treatment Strategies and New Approaches. Eurasian J Med 2017;49:53-8.
- 7. Thompson KD, Welykyj S, Massa MC. Antibacterial activity of lidocaine in combination with a bicarbonate buffer. J Dermatol Surg Oncol 1993;19:216-20.
- 8. Farha MA, French S, Stokes JM, Brown ED. Bicarbonate Alters Bacterial Susceptibility to Antibiotics by Targeting the Proton Motive Force. ACS Infect Dis 2018 9;4:382-90.
- 9. Begec Z, Gulhas N, Toprak HI, Yetkin G, et al. Comparison of the antibacterial activity of lidocaine 1% versus alkalinized lidocaine in vitro. Curr Ther Res Clin Exp 2007;68:242-8.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Orjinal Araştırma |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Eylül 2019 |
| Kabul Tarihi | 11 Eylül 2019 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 9 Sayı: 3 |