Öz
Objective: The treatment of Candida infections differs according to Candida species. The identification by routine and conventional methods can take several days. For this reason, rapid diagnosis of Candida species is important for initiating an effective antifungal therapy.
Material and Method: Sixty one Candida strains included in the study were evaluated at 24th and 48th hours after they were cultivated on CHROMagar Candida medium and incubated under appropriate conditions in accordance with the manufacturer's recommendations.
Results: When CHROMagar Candida medium and reference methods were compared in the study, it was found that CHROMagar Candida and referance method were detected the same species in 36 of the 61 samples (40.9%). Candida species were produced in CHROMagar Candida medium in 25 samples (59.1%) but the identification of species could not be made.
Conclusion: The CHROMagar Candida medium allows the discrimination of Candida parapsilosis species as well as Candida albicans according to the germe tube test, but does not allow the discrimination of other Candida species. It also has disadvantages of having problems due to different colors and high cost.
Anahtar Kelimeler
Kaynakça
- 1. Pfaller MA, Huston A, Coffmn S. Application of CHROMagar candida medium for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei, and Candida (Torulopsis) glabrata. J Clin Microbiol 1996; 34: 56-61.
- 2. Odds FC, Bernaerts R. CHROMagar Candida medium, a new differential isolation medium for presumptive identification of clinically important Candida species J Clin Microbiol 1994; 32: 1923-9.
- 3. Beighton D, Ludford R, Clark DT,et al. Use of CHROMagar Candida medium for isolation of yeasts from dental samples. J Clin Microbiol 1995; 32: 3025-7.
- 4. Kadri O, Ilkit M, Ates A, Bicer AT, Demirhindi H. Performance of Chromogenic Candida Agar and CHROMagar Candida in recovery and presumptive identifi cation of monofungal and polyfungal vaginal isolates. Medical Mycology February 2010, 48, 29–34.
- 5. Bishop JA, Chase N, Lee R, Kurtzman CP, Merz WG. Production of white colonies on CHROMagar Candida Medium by members of the Candida glabrata clade and other species with overlapping phenotypic traits. J Clin Microbiol 2008: 3498–500.
- 6. Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH. (ed.). Manual of Clinical Microbiology, 8th ed. American Society for Microbiology, Washington, D.C. 2003.
- 7. Schoofs A, Odds FC, Coleblunders R, Ieven M, Goosens H. Use of specialised isolation media for recognition and identification of Candida dubliniensis isolates from HIV-infected patients. Eur J Clin Microbiol Infect Dis 1997; 16: 296-300.
- 8. Kirkpatrick WR, Revankar SG, McAtee RK, et al. Detection of Candida dubliensis in oropharyngeal samples from Human Immunodeficiency Virusinfected patients in North America by primary CHROMagar Candida Medium screening and susceptibility testing of isolates. J Clin Microbiol 1998; 36: 3007-12.
- 9. Sahand IH, Moragues MD, Eraso E, Villar-Vidal M, Quindós G, Pontón J. Supplementation of CHROMagar Candida medium with Pal's medium for rapid identification of Candida dubliniensis. J Clin Microbiol 2005; 43: 5768-70.
- 10. Sivakumar VG, Shankar P, Nalina K, Menon T. Use of CHROMagar in the differentiation of common species of Candida. Mycopathologia 2009 Jan; 167: 47-9.
Öz
Amaç: Candida enfeksiyonlarının
tedavisi Candida türlerine göre
farklılık göstermektedir. Rutin ve klasik yöntemlerle identifikasyon birkaç
günü alabilmektedir. Bu nedenle, etkili antifungal tedavinin başlanabilmesi
için Candida türlerinin hızlı tanısı
önemlidir.
Gereç ve Yöntem: Çalışmaya dahil edilen 61 Candida
suşu CHROMagar Candida besiyerine ekilip üreticinin önerileri doğrultusunda uygun
şartlarda inkübe edildikten sonra, 24.
ve 48. saatlerde değerlendirildi.
Bulgular: Çalışmada CHROMagar Candida
besiyeri ile referans yöntemler karşılaştırıldığında, CHROMagar Candida
ile 61 örneğin 36’sında (%40,9)
referans yöntemle aynı tür tayini saptanmıştır. Yirmi beş örnekte
(%59,1) ise CHROMagar Candida besiyerinde Candida türleri üremiş ancak tür tayini yapılamamıştır.
Sonuç: CHROMagar Candida besiyeri germ tüp testine göre Candida albicans‘ın
yanısıra, Candida parapsilosis türlerinin ayrımına imkan
sağlamasına rağmen, diğer Candida türlerin
ayrımına imkan sağlamaması, farklı renkler nedeniyle sorunlar yaşanması ve
maliyetinin yüksek olması dezavantajlarıdır.
Anahtar Kelimeler
Kaynakça
- 1. Pfaller MA, Huston A, Coffmn S. Application of CHROMagar candida medium for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei, and Candida (Torulopsis) glabrata. J Clin Microbiol 1996; 34: 56-61.
- 2. Odds FC, Bernaerts R. CHROMagar Candida medium, a new differential isolation medium for presumptive identification of clinically important Candida species J Clin Microbiol 1994; 32: 1923-9.
- 3. Beighton D, Ludford R, Clark DT,et al. Use of CHROMagar Candida medium for isolation of yeasts from dental samples. J Clin Microbiol 1995; 32: 3025-7.
- 4. Kadri O, Ilkit M, Ates A, Bicer AT, Demirhindi H. Performance of Chromogenic Candida Agar and CHROMagar Candida in recovery and presumptive identifi cation of monofungal and polyfungal vaginal isolates. Medical Mycology February 2010, 48, 29–34.
- 5. Bishop JA, Chase N, Lee R, Kurtzman CP, Merz WG. Production of white colonies on CHROMagar Candida Medium by members of the Candida glabrata clade and other species with overlapping phenotypic traits. J Clin Microbiol 2008: 3498–500.
- 6. Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH. (ed.). Manual of Clinical Microbiology, 8th ed. American Society for Microbiology, Washington, D.C. 2003.
- 7. Schoofs A, Odds FC, Coleblunders R, Ieven M, Goosens H. Use of specialised isolation media for recognition and identification of Candida dubliniensis isolates from HIV-infected patients. Eur J Clin Microbiol Infect Dis 1997; 16: 296-300.
- 8. Kirkpatrick WR, Revankar SG, McAtee RK, et al. Detection of Candida dubliensis in oropharyngeal samples from Human Immunodeficiency Virusinfected patients in North America by primary CHROMagar Candida Medium screening and susceptibility testing of isolates. J Clin Microbiol 1998; 36: 3007-12.
- 9. Sahand IH, Moragues MD, Eraso E, Villar-Vidal M, Quindós G, Pontón J. Supplementation of CHROMagar Candida medium with Pal's medium for rapid identification of Candida dubliniensis. J Clin Microbiol 2005; 43: 5768-70.
- 10. Sivakumar VG, Shankar P, Nalina K, Menon T. Use of CHROMagar in the differentiation of common species of Candida. Mycopathologia 2009 Jan; 167: 47-9.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Orijinal Makale |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Eylül 2018 |
| Yayımlandığı Sayı | Yıl 2018 Cilt: 1 Sayı: 3 |
Üniversitelerarası Kurul (ÜAK) Eşdeğerliği: Ulakbim TR Dizin'de olan dergilerde yayımlanan makale [10 PUAN] ve 1a, b, c hariç uluslararası indekslerde (1d) olan dergilerde yayımlanan makale [5 PUAN]
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Not: Dergimiz WOS indeksli değildir ve bu nedenle Q olarak sınıflandırılmamıştır.
Yüksek Öğretim Kurumu (YÖK) kriterlerine göre yağmacı/şüpheli dergiler hakkındaki kararları ile yazar aydınlatma metni ve dergi ücretlendirme politikasını tarayıcınızdan indirebilirsiniz. https://dergipark.org.tr/tr/journal/2316/file/4905/show
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