Araştırma Makalesi
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Yıl 2019, Cilt: 4 Sayı: 3, 84 - 90, 30.09.2019

Ayşegül Aksoy Gökmen Aylin Babaoğlu Bayram Pektaş Pektaş Selçuk Kaya Derya Dirim Erdoğan Metin Korkmaz

Öz

Kaynakça

  • Azizi S, Oryan A, Sadjjadi SM, Zibaei M. Histopathologic changes and larval recovery of Toxocara cati in experimentally infected chickens. Parasitol Res. 2007; 102 (1): 47–52.
  • Roldán WH, Espinoza YA, Atúncar A, Ortega E, Martinez A, Saravia M. Frequency of eosinophilia and risk factors and their association with Toxocara infection in schoolchildren during a health survey in the north of Lima, Peru. Rev Inst Med Trop Sao Paulo. 2008; 50(5):273-278.
  • Magnaval JF, Glickman LT, Dorchies P, Morassin B. Highlights of human toxocariasis. Korean J Parasitol. 2001; 39(1):1-11.
  • Zibaei M, Sadjjadi SM. Trend of toxocariasis in Iran: a review on human and animal dimensions. Iran J vet Res. 2017; 18(4): 233-242.
  • Öge H, Öge S, Özbakış G, Gürcan S. Comparison of Toxocara eggs in hair and faecal samples from owned dogs and cats collected in Ankara, Turkey. Vet Parasitol. 2014; 206(3-4):227-31.
  • Cooper PJ, Alexander N, Moncayo AL, Benitez SM, Chico ME, Vaca MG, Griffin GE. Environmental determinants of total IgE among school children living in the rural Tropics: importance of geohelminth infections and effect of anthelmintic treatment. BMC Immunol. 2008; 27:9:33.
  • Dattoli VC, Freire SM, Mendonça LR, Santos PC, Meyer R, Alcantara-Neves NM. Toxocara canis infection is associated with eosinophilia and total IgE in blood donors from a large Brazilian centre. Trop Med Int Health. 2011; 16(4):514-7.
  • Iddawela D, Ehambaram K, Atapattu D, Pethiyagoda K, Bandara L. Frequency of Toxocariasis among Patients Clinically Suspected to Have Visceral Toxocariasis: A Retrospective Descriptive Study in Sri Lanka. J Parasitol Res. 2017; 4368659.
  • Mohamad S, Azmi NC, Noordin R. Development and evaluation of a sensitive and specific assay for diagnosis of human toxocariasis by use of three recombinant antigens (TES-26, TES-30USM, and TES-120). Clin Microbiol 2009; 47(6): 1712-7.
  • Yamasaki H, Araki K, Lim PK, et al. Development of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory antigen for immunodiagnosis of human toxocariasis. J Clin Microbiol 2000; 38(4): 1409-13.
  • Perteguer MJ, Cuéllar C, Guillén JL, et al. Cross-reactivity between Anisakis simplex sensitization and visceral larva migrans by Toxocara canis. Acta Trop 2003; 89(1): 85-9.
  • Rodero M, Chivato T, Muro A, Cuéllar C. Enzyme-linked immunosorbent assay and Western blot antibody determination in sera from patients diagnosed with different helminthic infections with Anisakis simplex antigen purified by affinity chromatography. Mem Inst Oswaldo Cruz 2005; 100(3): 293-301.
  • Jacquier P, Gottstein B, Stingelin Y, Eckert J. Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit. Clin Microbiol 1991; 29(9): 1831-5.
  • Korkmaz M. Visceral larva migrans. İkinci evre Toxocara canis larvalarının in vitro kültürü, ekskretuvar/sekretuvar antijenin elde edilmesi ve ELISA yöntemi ile tanısı (Uzmanlık tezi). Ege Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, İzmir. (1998).
  • Schägger H1, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987 Nov 1;166(2):368-79.
  • Magnaval JF, Fabre R, Maurières P, Charlet JP, de Larrard B. Application of the western blotting procedure for the immunodiagnosis of human toxocariasis. Parasitol Res. 1991;77(8):697-702.
  • Sözen H, Citil BE, Caylak S, Gokmen AA, Kaya S, Demirci M, Korkmaz M, Sahin C, Kirli I. Seroepidemiological Study of Toxocariasis among Volunteers Animal Husbandry Workers and Veterinary in Southern Anatolia in Turkey in 2014. Iran J Parasitol. 2015; 10(3):473-81.
  • Yazar S, Yaman O, Cetinkaya U, Hamamci B, Sahin I. Investigation of anti-Toxocara canis IgG antibodies in patients presenting at The Erciyes University Medical Faculty, Department of Parasitology. Turkiye Parazitol Derg. 2010; 34(1):24-26.
  • Doğan N, Dinleyici EC, Bor O, Töz SO, Ozbel Y. Seroepidemiological survey for Toxocara canis infection in the northwestern part of Turkey. Turkiye Parazitol Derg. 2007; 31(4):288-91.
  • Büyükbaba Ö, Özkan E, Büget E. Toxocara canis ve çocuklardaki prevalansının ELISA ile araştırılması. İnfeksiyon Derg. 1996; 10(1): 7-11.
  • Kwon NH, Oh MJ, Lee SP, Lee BJ, Choi DC. The prevalence and diagnostic value of toxocariasis in unknown eosinophilia. Ann Hematol. 2006; 85(4):233-8.
  • Demirci M, Kaya S, Cetin E, Arıdoğan B, Onal S, Korkmaz M. Seroepidemiological investigation of toxocariasis in the isparta region of Turkey. Iran J Parasitol. 2010; 5(2):52-59.
  • Kim HB, Seo JW2, Lee JH2, Choi BS3, Park SG2. Evaluation of the prevalence and clinical impact of toxocariasis in patients with eosinophilia of unknown origin. Korean J Intern Med. 2017; 32(3):523-529.
  • Maraghi S, Rafiei A, Hajihossein R, Sadjjadi SM. Seroprevalence of toxocariasis in hypereosinophilic individuals in Ahwaz, south-western Iran. J Helminthol. 2012; 86(2): 241-244.

High Eosinophilia Strongly Associated with Human Toxocariasis

Yıl 2019, Cilt: 4 Sayı: 3, 84 - 90, 30.09.2019

Ayşegül Aksoy Gökmen Aylin Babaoğlu Bayram Pektaş Pektaş Selçuk Kaya Derya Dirim Erdoğan Metin Korkmaz

Öz

Backround: The aim of this study was to reveal the prevalence of toxocariasis in
patients with eosinophilia using Western blotting, and to show the importance
of eosinophilia for toxocariasis. Materials and
Methods: For this aim, 122 individuals, of whom 37 was without eosinophilia, were included to the study and in
their serum sample, presence of IgG against Toxocara
canis was investigated by Western
blotting. Patients with eosinophilia were divided into two groups. In the first group (n=52), eosinophilic cells constitute 3% to 10% of total
white cells (<10% ) whereas in the second group (n: 33) eosinophilic cells
constitute greater  than or equal to 10%
of total white cells (≥10%) . In addition, the cut-off value of the control group
(n=37) for eosinophilia was ≤3% of total white cells.



Results: According to the results that obtained
from the study, 62.3% (n=76/122) of all patients (including healthy people)
were found seropositive. Also, 97% of (n=32/33) patient serum samples with ≥10 eosinophilia
and 46.1% (n=24/52) of patient serum samples with <10% eosinophilia were found
to be positive when patients were categorized depending on eosinophilia levels.
Among control group serum samples with ≤3% eosinophilia (n=37), 54% of them
were detected to be positive.



Conclusion: Accordingly, the seropositivity
rate detected in patients with at least 10% eosinophilia was statistically
significant when compared with the other groups (P<0.05). Seropositivity values detected in patients with
eosinophilia are notable. Therefore, physicians should keep Toxocara spp. infection in mind in
patients with eosinophilia. 

Anahtar Kelimeler

Toxocara spp. human toxocariasis

Kaynakça

  • Azizi S, Oryan A, Sadjjadi SM, Zibaei M. Histopathologic changes and larval recovery of Toxocara cati in experimentally infected chickens. Parasitol Res. 2007; 102 (1): 47–52.
  • Roldán WH, Espinoza YA, Atúncar A, Ortega E, Martinez A, Saravia M. Frequency of eosinophilia and risk factors and their association with Toxocara infection in schoolchildren during a health survey in the north of Lima, Peru. Rev Inst Med Trop Sao Paulo. 2008; 50(5):273-278.
  • Magnaval JF, Glickman LT, Dorchies P, Morassin B. Highlights of human toxocariasis. Korean J Parasitol. 2001; 39(1):1-11.
  • Zibaei M, Sadjjadi SM. Trend of toxocariasis in Iran: a review on human and animal dimensions. Iran J vet Res. 2017; 18(4): 233-242.
  • Öge H, Öge S, Özbakış G, Gürcan S. Comparison of Toxocara eggs in hair and faecal samples from owned dogs and cats collected in Ankara, Turkey. Vet Parasitol. 2014; 206(3-4):227-31.
  • Cooper PJ, Alexander N, Moncayo AL, Benitez SM, Chico ME, Vaca MG, Griffin GE. Environmental determinants of total IgE among school children living in the rural Tropics: importance of geohelminth infections and effect of anthelmintic treatment. BMC Immunol. 2008; 27:9:33.
  • Dattoli VC, Freire SM, Mendonça LR, Santos PC, Meyer R, Alcantara-Neves NM. Toxocara canis infection is associated with eosinophilia and total IgE in blood donors from a large Brazilian centre. Trop Med Int Health. 2011; 16(4):514-7.
  • Iddawela D, Ehambaram K, Atapattu D, Pethiyagoda K, Bandara L. Frequency of Toxocariasis among Patients Clinically Suspected to Have Visceral Toxocariasis: A Retrospective Descriptive Study in Sri Lanka. J Parasitol Res. 2017; 4368659.
  • Mohamad S, Azmi NC, Noordin R. Development and evaluation of a sensitive and specific assay for diagnosis of human toxocariasis by use of three recombinant antigens (TES-26, TES-30USM, and TES-120). Clin Microbiol 2009; 47(6): 1712-7.
  • Yamasaki H, Araki K, Lim PK, et al. Development of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory antigen for immunodiagnosis of human toxocariasis. J Clin Microbiol 2000; 38(4): 1409-13.
  • Perteguer MJ, Cuéllar C, Guillén JL, et al. Cross-reactivity between Anisakis simplex sensitization and visceral larva migrans by Toxocara canis. Acta Trop 2003; 89(1): 85-9.
  • Rodero M, Chivato T, Muro A, Cuéllar C. Enzyme-linked immunosorbent assay and Western blot antibody determination in sera from patients diagnosed with different helminthic infections with Anisakis simplex antigen purified by affinity chromatography. Mem Inst Oswaldo Cruz 2005; 100(3): 293-301.
  • Jacquier P, Gottstein B, Stingelin Y, Eckert J. Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit. Clin Microbiol 1991; 29(9): 1831-5.
  • Korkmaz M. Visceral larva migrans. İkinci evre Toxocara canis larvalarının in vitro kültürü, ekskretuvar/sekretuvar antijenin elde edilmesi ve ELISA yöntemi ile tanısı (Uzmanlık tezi). Ege Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, İzmir. (1998).
  • Schägger H1, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987 Nov 1;166(2):368-79.
  • Magnaval JF, Fabre R, Maurières P, Charlet JP, de Larrard B. Application of the western blotting procedure for the immunodiagnosis of human toxocariasis. Parasitol Res. 1991;77(8):697-702.
  • Sözen H, Citil BE, Caylak S, Gokmen AA, Kaya S, Demirci M, Korkmaz M, Sahin C, Kirli I. Seroepidemiological Study of Toxocariasis among Volunteers Animal Husbandry Workers and Veterinary in Southern Anatolia in Turkey in 2014. Iran J Parasitol. 2015; 10(3):473-81.
  • Yazar S, Yaman O, Cetinkaya U, Hamamci B, Sahin I. Investigation of anti-Toxocara canis IgG antibodies in patients presenting at The Erciyes University Medical Faculty, Department of Parasitology. Turkiye Parazitol Derg. 2010; 34(1):24-26.
  • Doğan N, Dinleyici EC, Bor O, Töz SO, Ozbel Y. Seroepidemiological survey for Toxocara canis infection in the northwestern part of Turkey. Turkiye Parazitol Derg. 2007; 31(4):288-91.
  • Büyükbaba Ö, Özkan E, Büget E. Toxocara canis ve çocuklardaki prevalansının ELISA ile araştırılması. İnfeksiyon Derg. 1996; 10(1): 7-11.
  • Kwon NH, Oh MJ, Lee SP, Lee BJ, Choi DC. The prevalence and diagnostic value of toxocariasis in unknown eosinophilia. Ann Hematol. 2006; 85(4):233-8.
  • Demirci M, Kaya S, Cetin E, Arıdoğan B, Onal S, Korkmaz M. Seroepidemiological investigation of toxocariasis in the isparta region of Turkey. Iran J Parasitol. 2010; 5(2):52-59.
  • Kim HB, Seo JW2, Lee JH2, Choi BS3, Park SG2. Evaluation of the prevalence and clinical impact of toxocariasis in patients with eosinophilia of unknown origin. Korean J Intern Med. 2017; 32(3):523-529.
  • Maraghi S, Rafiei A, Hajihossein R, Sadjjadi SM. Seroprevalence of toxocariasis in hypereosinophilic individuals in Ahwaz, south-western Iran. J Helminthol. 2012; 86(2): 241-244.
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Tıp Bilimleri
Bölüm Araştırma Makaleleri
Yazarlar

Ayşegül Aksoy Gökmen

Aylin Babaoğlu Bu kişi benim

Bayram Pektaş Pektaş

Selçuk Kaya

Derya Dirim Erdoğan Bu kişi benim

Metin Korkmaz Bu kişi benim

Yayımlanma Tarihi 30 Eylül 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 4 Sayı: 3

Kaynak Göster

APA Aksoy Gökmen, A., Babaoğlu, A., Pektaş, B. P., Kaya, S., vd. (2019). High Eosinophilia Strongly Associated with Human Toxocariasis. Journal of Immunology and Clinical Microbiology, 4(3), 84-90.
AMA Aksoy Gökmen A, Babaoğlu A, Pektaş BP, Kaya S, Dirim Erdoğan D, Korkmaz M. High Eosinophilia Strongly Associated with Human Toxocariasis. J Immunol Clin Microbiol. Eylül 2019;4(3):84-90.
Chicago Aksoy Gökmen, Ayşegül, Aylin Babaoğlu, Bayram Pektaş Pektaş, Selçuk Kaya, Derya Dirim Erdoğan, ve Metin Korkmaz. “High Eosinophilia Strongly Associated With Human Toxocariasis”. Journal of Immunology and Clinical Microbiology 4, sy. 3 (Eylül 2019): 84-90.
EndNote Aksoy Gökmen A, Babaoğlu A, Pektaş BP, Kaya S, Dirim Erdoğan D, Korkmaz M (01 Eylül 2019) High Eosinophilia Strongly Associated with Human Toxocariasis. Journal of Immunology and Clinical Microbiology 4 3 84–90.
IEEE A. Aksoy Gökmen, A. Babaoğlu, B. P. Pektaş, S. Kaya, D. Dirim Erdoğan, ve M. Korkmaz, “High Eosinophilia Strongly Associated with Human Toxocariasis”, J Immunol Clin Microbiol, c. 4, sy. 3, ss. 84–90, 2019.
ISNAD Aksoy Gökmen, Ayşegül vd. “High Eosinophilia Strongly Associated With Human Toxocariasis”. Journal of Immunology and Clinical Microbiology 4/3 (Eylül 2019), 84-90.
JAMA Aksoy Gökmen A, Babaoğlu A, Pektaş BP, Kaya S, Dirim Erdoğan D, Korkmaz M. High Eosinophilia Strongly Associated with Human Toxocariasis. J Immunol Clin Microbiol. 2019;4:84–90.
MLA Aksoy Gökmen, Ayşegül vd. “High Eosinophilia Strongly Associated With Human Toxocariasis”. Journal of Immunology and Clinical Microbiology, c. 4, sy. 3, 2019, ss. 84-90.
Vancouver Aksoy Gökmen A, Babaoğlu A, Pektaş BP, Kaya S, Dirim Erdoğan D, Korkmaz M. High Eosinophilia Strongly Associated with Human Toxocariasis. J Immunol Clin Microbiol. 2019;4(3):84-90.

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