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Atmosferik basınç soğuk plazmanın bakteri izolatları üzerine etkilerinin araştırılması

Yıl 2023, Cilt: 2 Sayı: 3, 65 - 69, 30.12.2023

Ferhat Bozduman Yusuf Seçgin Şerife Yılmaz Zülal Öner Hasan Solmaz

https://doi.org/10.58651/jomtu.1390655

Öz

Amaç: Atmosferik basınç soğuk plazma sağlık, gıda ve tarım sektöründe antimikrobiyal etkinliği için kullanılan termal olmayan plazma türüdür. Bu çalışma farklı bakteri izolatları üzerine atmosferik basınç soğuk plazmanın etkinliğinin araştırılması amacıyla gerçekleştirildi.
Materyal ve Metot: Çalışma standart referans bakteri suşu olan; Escherichia coli (O157:H7), Bacillus subtilis (ATCC 6633), Enterobacter aerogenes (ATCC 13048), Rhodococcus equi (ATCC 6939), Salmonella typhimurium (ATCC 14028), Enterococcus feacalis (ATCC 2912) ve saha izolatı olan 12 adet Escherichia coli izolatı, 22 adet Staphylococcus spp., 18 adet Klebsiella spp., 2 adet Enterococcus spp., 3 adet Acinetobacter spp., 8 adet Candida spp., 1 adet Morgarella mongarii, 2 adet Corynebacterium spp., 1 adet Streptococcus pyogenes üzerine gerçekleştirildi. İzolatlara atmosferik basınç soğuk plazma jeti ve plazma ile aktive edilmiş ortam (PAM) uygulandı ve etkinliği araştırıldı.
Bulgular: Çalışma sonucunda standart bakteri suşlarında Escherichia coli (O157:H7), Enterobacter aerogenes (ATCC 13048), Salmonella typhimurium, Rhodococcus equi (ATCC 6939) ve Enterococcus feacalis (ATCC 2912)’e soğuk plazmanın etki ettiği, saha izolatlarından ise Streptococcus pyogenes (A grubuBet) ile Staphylococcus epidermidis’e etki gösterdiği bulundu.
Sonuç: Çalışmamız sonucunda atmosferik basınç soğuk plazmanın ve PAM’ın antimikrobiyal etkinliği ortaya konuldu ve bu yönüyle sağlık, gıda ve tarım sektörlerine katkı sağlayacağını düşünmekteyiz.

Anahtar Kelimeler

Atmosferik Basınç Soğuk Plazma Plazma ile Aktive Edilmiş Ortam Bakteri İzolatı Mikrobiyal Etkinlik

Proje Numarası

KBUBAP-22-DS-125

Kaynakça

  • Balcı, S. K., Kutlu, S.N., Temel, U. B. & Güleç, A. (2021). Atmosferik basınç soğuk plazmanın diş hekimliğindeki uygulamaları. Research Journal of Biomedical and Biotechnology, 2(1), 1-9.
  • Bernhardt, T., Semmler, M.L., Schäfer, M., Bekeschus, S., Emmert, S. & Boeckmann, L. (2019). Plasma medicine: applications of cold atmospheric pressure plasma in dermatology. Oxidative medicine and cellular longevity, 2019.
  • Branchu, P., Bawn, M. & Kingsley, R.A. (2018). Genome variation and molecular epidemiology of Salmonella enterica serovar typhimurium pathovariants. Infection and immunity, 86(8), 10.1128/iai. 00079-00018.
  • Brun, P., Brun, P., Vono, M., Venier, P., Tarricone, E., Deligianni, V., . . . Cavazzana, R. (2012). Disinfection of ocular cells and tissues by atmospheric-pressure cold plasma. Plos one, 7(3), e33245.
  • Cha, S. & Park, Y.-S. (2014). Plasma in dentistry. Clinical plasma medicine, 2(1), 4-10.
  • Cheng, Y.-J., Lin, C.-K., Chen, C.-Y., Chien, P.-C., Chuan, H.-H., Ho, C.-C. & Cheng, Y.-C. (2020). Plasma-activated medium as adjuvant therapy for lung cancer with malignant pleural effusion. Scientific Reports, 10(1), 18154.
  • Ermolaeva, S.A., Varfolomeev, A.F., Chernukha, M. Y., Yurov, D.S., Vasiliev, M.M., Kaminskaya, A. A., . . . & Selezneva, I.I. (2011). Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds. Journal of medical microbiology, 60(1), 75-83.
  • Foster, T. (1996). Staphylococcus. Medical Microbiology. 4th edition.
  • Gurol, C., Ekinci, F., Aslan, N. & Korachi, M. (2012). Low temperature plasma for decontamination of E. coli in milk. International journal of food microbiology, 157(1), 1-5.
  • Heinlin, J., Isbary, G., Stolz, W., Morfill, G., Landthaler, M., Shimizu, T., . . . & Karrer, S. (2011). Plasma applications in medicine with a special focus on dermatology. Journal of the European Academy of Dermatology and Venereology, 25(1), 1-11.
  • Hoffmann, C., Berganza, C. & Zhang, J. (2013). Cold atmospheric plasma: methods of production and application in dentistry and oncology. Medical gas research, 3(1), 1-15.
  • Hondalus, M. K. & Mosser, D.M. (1994). Survival and replication of rhodococcus equi in macrophages. Infection and immunity, 62(10), 4167-4175.
  • Hosseini, S.M., Rostami, S., Hosseinzadeh Samani, B. & Lorigooini, Z. (2020). The effect of atmospheric pressure cold plasma on the inactivation of Escherichia coli in sour cherry juice and its qualitative properties. Food Science & Nutrition, 8(2), 870-883.
  • Isbary, G., Shimizu, T., Li, Y.-F., Stolz, W., Thomas, H. M., Morfill, G. E. & Zimmermann, J.L. (2013). Cold atmospheric plasma devices for medical issues. Expert review of medical devices, 10(3), 367-377.
  • Kandemir, H., AYDIN, F., Güler, B. & Gürel, A. (2021). Soğuk plazma teknolojisi ve tarımdaki çeşitli uygulama alanları. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 35(1), 217-245.
  • Laroussi, M. (2002). Nonthermal decontamination of biological media by atmospheric-pressure plasmas: review, analysis, and prospects. IEEE Transactions on plasma science, 30(4), 1409-1415.
  • Li, Y., Sun, K., Ye, G., Liang, Y., Pan, H., Wang, G., . . . Fang, J. (2015). Evaluation of cold plasma treatment and safety in disinfecting 3-week root canal Enterococcus faecalis biofilm in vitro. Journal of endodontics, 41(8), 1325-1330.
  • Malléa, M., Chevalier, J., Eyraud, A. & Pagès, J.-M. (2002). Inhibitors of antibiotic efflux pump in resistant Enterobacter aerogenes strains. Biochemical and biophysical research communications, 293(5), 1370-1373. McBride, S.M., Fischetti, V.A., LeBlanc, D.J., Moellering Jr, R.C. & Gilmore, M.S. (2007). Genetic diversity among enterococcus faecalis. Plos one, 2(7), e582.
  • Mead, P.S. & Griffin, P.M. (1998). Escherichia coli O157: H7. The Lancet, 352(9135), 1207-1212. Munoz-Price, L.S. & Weinstein, R.A. (2008). Acinetobacter infection. New England Journal of Medicine, 358(12), 1271-1281.
  • Oggioni, M.R., Pozzi, G., Valensin, P.E., Galieni, P. & Bigazzi, C. (1998). Recurrent septicemia in an immunocompromised patient due to probiotic strains of Bacillus subtilis. Journal of clinical microbiology, 36(1), 325. Oztan, M. O., Ercan, U.K., Aksoy Gokmen, A., Simsek, F., Ozdemir, G.D. & Koyluoglu, G. (2022). Irrigation of peritoneal cavity with cold atmospheric plasma treated solution effectively reduces microbial load in rat acute peritonitis model. Scientific Reports, 12(1), 3646. Pennington, H. (2010). Escherichia coli O157. The Lancet, 376(9750), 1428-1435.
  • Ristuccia, P.A. & Cunha, B.A. (1984). Klebsiella. Infection Control & Hospital Epidemiology, 5(7), 343-347.
  • Usta, Y.H. & Ercan, U.K. (2017) Enfekte Yara Yönetimi İçin Portatif Soğuk Atmosferik Plazma Cihazının Geliştirilmesi. Medical Technologies National Congress (TIPTEKNO), 174-177.
  • Vecchio, D., Dai, T., Huang, L., Fantetti, L., Roncucci, G. & Hamblin, M.R. (2013). Antimicrobial photodynamic therapy with RLP068 kills methicillin‐resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion. Journal of biophotonics, 6(9), 733-742.
  • Yang, B., Chen, J., Yu, Q., Li, H., Lin, M., Mustapha, A., . . . Wang, Y. (2011). Oral bacterial deactivation using a low-temperature atmospheric argon plasma brush. Journal of dentistry, 39(1), 48-56. Yong, H.I., Kim, H.-J., Park, S., Alahakoon, A.U., Kim, K., Choe, W. & Jo, C. (2015). Evaluation of pathogen inactivation on sliced cheese induced by encapsulated atmospheric pressure dielectric barrier discharge plasma. Food Microbiology, 46, 46-50.
  • Yüksel, Ç.Y. & Karagözlü, N. (2017). Soğuk atmosferik plazma teknolojisi ve gıdalarda kullanımı. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 14(2), 81-86.

Investigation of the effects of atmospheric pressure cold plasma on bacterial isolates

Yıl 2023, Cilt: 2 Sayı: 3, 65 - 69, 30.12.2023

Ferhat Bozduman Yusuf Seçgin Şerife Yılmaz Zülal Öner Hasan Solmaz

https://doi.org/10.58651/jomtu.1390655

Öz

Background: Atmospheric pressure cold plasma is a type of non-thermal plasma used for antimicrobial activity in the health, food and agriculture sectors. This study was carried out to investigate the efficacy of atmospheric pressure cold plasma on different bacterial isolates.
Materials and Methods: The study was conducted on standard reference bacterial strains; Escherichia coli (O157:H7), Bacillus subtilis (ATCC 6633), Enterobacter aerogenes (ATCC 13048), Rhodococcus equi (ATCC 6939), Salmonella typhimurium (ATCC 14028), Enterococcus feacalis (ATCC 2912) and field isolates; 12 Escherichia coli isolates, 22 Staphylococcus spp, 18 Klebsiella spp., 2 Enterococcus spp., 3 Acinetobacter spp., 8 Candida spp., 1 Morgarella mongarii, 2 Corynebacterium spp., 1 Streptococcus pyogenes. Atmospheric pressure cold plasma jet and plasma activated medium (PAM) were applied to the isolates and their efficacy was investigated.
Results: As a result of the study, it was found that cold plasma was effective against Escherichia coli (O157:H7), Enterobacter aerogenes (ATCC 13048), Salmonella typhimurium, Rhodococcus equi (ATCC 6939) and Enterococcus feacalis (ATCC 2912) among standard bacterial strains, while it was effective against Streptococcus pyogenes (group A Bet) and Staphylococcus epidermidis among field isolates.
Conclusions: As a result of our study, the antimicrobial activity of atmospheric pressure cold plasma and PAM was demonstrated, and we believe that it will contribute to the health, food and agriculture sectors.

Anahtar Kelimeler

Atmospheric Pressure Cold Plasma Plasma Activated Media Bacterial Isolate Microbial Activity

Etik Beyan

This study was approved by Karabük University Interventional Ethics Committee with the decision number 2022/1068.

Destekleyen Kurum

This study was supported by Karabuk University Scientific Research Projects Coordination Office. Project Number: KBUBAP-22-DS-125.

Proje Numarası

KBUBAP-22-DS-125

Teşekkür

This study was supported by Karabuk University Scientific Research Projects Coordination Office. Project Number: KBUBAP-22-DS-125. We express our thanks.

Kaynakça

  • Balcı, S. K., Kutlu, S.N., Temel, U. B. & Güleç, A. (2021). Atmosferik basınç soğuk plazmanın diş hekimliğindeki uygulamaları. Research Journal of Biomedical and Biotechnology, 2(1), 1-9.
  • Bernhardt, T., Semmler, M.L., Schäfer, M., Bekeschus, S., Emmert, S. & Boeckmann, L. (2019). Plasma medicine: applications of cold atmospheric pressure plasma in dermatology. Oxidative medicine and cellular longevity, 2019.
  • Branchu, P., Bawn, M. & Kingsley, R.A. (2018). Genome variation and molecular epidemiology of Salmonella enterica serovar typhimurium pathovariants. Infection and immunity, 86(8), 10.1128/iai. 00079-00018.
  • Brun, P., Brun, P., Vono, M., Venier, P., Tarricone, E., Deligianni, V., . . . Cavazzana, R. (2012). Disinfection of ocular cells and tissues by atmospheric-pressure cold plasma. Plos one, 7(3), e33245.
  • Cha, S. & Park, Y.-S. (2014). Plasma in dentistry. Clinical plasma medicine, 2(1), 4-10.
  • Cheng, Y.-J., Lin, C.-K., Chen, C.-Y., Chien, P.-C., Chuan, H.-H., Ho, C.-C. & Cheng, Y.-C. (2020). Plasma-activated medium as adjuvant therapy for lung cancer with malignant pleural effusion. Scientific Reports, 10(1), 18154.
  • Ermolaeva, S.A., Varfolomeev, A.F., Chernukha, M. Y., Yurov, D.S., Vasiliev, M.M., Kaminskaya, A. A., . . . & Selezneva, I.I. (2011). Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds. Journal of medical microbiology, 60(1), 75-83.
  • Foster, T. (1996). Staphylococcus. Medical Microbiology. 4th edition.
  • Gurol, C., Ekinci, F., Aslan, N. & Korachi, M. (2012). Low temperature plasma for decontamination of E. coli in milk. International journal of food microbiology, 157(1), 1-5.
  • Heinlin, J., Isbary, G., Stolz, W., Morfill, G., Landthaler, M., Shimizu, T., . . . & Karrer, S. (2011). Plasma applications in medicine with a special focus on dermatology. Journal of the European Academy of Dermatology and Venereology, 25(1), 1-11.
  • Hoffmann, C., Berganza, C. & Zhang, J. (2013). Cold atmospheric plasma: methods of production and application in dentistry and oncology. Medical gas research, 3(1), 1-15.
  • Hondalus, M. K. & Mosser, D.M. (1994). Survival and replication of rhodococcus equi in macrophages. Infection and immunity, 62(10), 4167-4175.
  • Hosseini, S.M., Rostami, S., Hosseinzadeh Samani, B. & Lorigooini, Z. (2020). The effect of atmospheric pressure cold plasma on the inactivation of Escherichia coli in sour cherry juice and its qualitative properties. Food Science & Nutrition, 8(2), 870-883.
  • Isbary, G., Shimizu, T., Li, Y.-F., Stolz, W., Thomas, H. M., Morfill, G. E. & Zimmermann, J.L. (2013). Cold atmospheric plasma devices for medical issues. Expert review of medical devices, 10(3), 367-377.
  • Kandemir, H., AYDIN, F., Güler, B. & Gürel, A. (2021). Soğuk plazma teknolojisi ve tarımdaki çeşitli uygulama alanları. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 35(1), 217-245.
  • Laroussi, M. (2002). Nonthermal decontamination of biological media by atmospheric-pressure plasmas: review, analysis, and prospects. IEEE Transactions on plasma science, 30(4), 1409-1415.
  • Li, Y., Sun, K., Ye, G., Liang, Y., Pan, H., Wang, G., . . . Fang, J. (2015). Evaluation of cold plasma treatment and safety in disinfecting 3-week root canal Enterococcus faecalis biofilm in vitro. Journal of endodontics, 41(8), 1325-1330.
  • Malléa, M., Chevalier, J., Eyraud, A. & Pagès, J.-M. (2002). Inhibitors of antibiotic efflux pump in resistant Enterobacter aerogenes strains. Biochemical and biophysical research communications, 293(5), 1370-1373. McBride, S.M., Fischetti, V.A., LeBlanc, D.J., Moellering Jr, R.C. & Gilmore, M.S. (2007). Genetic diversity among enterococcus faecalis. Plos one, 2(7), e582.
  • Mead, P.S. & Griffin, P.M. (1998). Escherichia coli O157: H7. The Lancet, 352(9135), 1207-1212. Munoz-Price, L.S. & Weinstein, R.A. (2008). Acinetobacter infection. New England Journal of Medicine, 358(12), 1271-1281.
  • Oggioni, M.R., Pozzi, G., Valensin, P.E., Galieni, P. & Bigazzi, C. (1998). Recurrent septicemia in an immunocompromised patient due to probiotic strains of Bacillus subtilis. Journal of clinical microbiology, 36(1), 325. Oztan, M. O., Ercan, U.K., Aksoy Gokmen, A., Simsek, F., Ozdemir, G.D. & Koyluoglu, G. (2022). Irrigation of peritoneal cavity with cold atmospheric plasma treated solution effectively reduces microbial load in rat acute peritonitis model. Scientific Reports, 12(1), 3646. Pennington, H. (2010). Escherichia coli O157. The Lancet, 376(9750), 1428-1435.
  • Ristuccia, P.A. & Cunha, B.A. (1984). Klebsiella. Infection Control & Hospital Epidemiology, 5(7), 343-347.
  • Usta, Y.H. & Ercan, U.K. (2017) Enfekte Yara Yönetimi İçin Portatif Soğuk Atmosferik Plazma Cihazının Geliştirilmesi. Medical Technologies National Congress (TIPTEKNO), 174-177.
  • Vecchio, D., Dai, T., Huang, L., Fantetti, L., Roncucci, G. & Hamblin, M.R. (2013). Antimicrobial photodynamic therapy with RLP068 kills methicillin‐resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion. Journal of biophotonics, 6(9), 733-742.
  • Yang, B., Chen, J., Yu, Q., Li, H., Lin, M., Mustapha, A., . . . Wang, Y. (2011). Oral bacterial deactivation using a low-temperature atmospheric argon plasma brush. Journal of dentistry, 39(1), 48-56. Yong, H.I., Kim, H.-J., Park, S., Alahakoon, A.U., Kim, K., Choe, W. & Jo, C. (2015). Evaluation of pathogen inactivation on sliced cheese induced by encapsulated atmospheric pressure dielectric barrier discharge plasma. Food Microbiology, 46, 46-50.
  • Yüksel, Ç.Y. & Karagözlü, N. (2017). Soğuk atmosferik plazma teknolojisi ve gıdalarda kullanımı. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 14(2), 81-86.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Tıbbi Bakteriyoloji
Bölüm Araştırma Makaleleri
Yazarlar

Ferhat Bozduman 0000-0002-2669-021X

Yusuf Seçgin 0000-0002-0118-6711

Şerife Yılmaz 0000-0001-5310-3933

Zülal Öner 0000-0003-0459-1015

Hasan Solmaz 0000-0002-6225-7579

Proje Numarası KBUBAP-22-DS-125
Yayımlanma Tarihi 30 Aralık 2023
Gönderilme Tarihi 14 Kasım 2023
Kabul Tarihi 5 Aralık 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 2 Sayı: 3

Kaynak Göster

APA Bozduman, F., Seçgin, Y., Yılmaz, Ş., Öner, Z., vd. (2023). Investigation of the effects of atmospheric pressure cold plasma on bacterial isolates. Journal of Medical Topics and Updates, 2(3), 65-69. https://doi.org/10.58651/jomtu.1390655
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