Araştırma Makalesi
BibTex RIS Kaynak Göster

Rosa canina özütü kullanılarak gümüş nanopartiküllerin biyosentezi ve insan kolon adenokarsinoma hücre hattı HT29 üzerindeki anti-kanser ve anti-metastatik aktivitesi

Yıl 2019, Cilt: 7 Sayı: 2, 124 - 131, 30.12.2019
https://doi.org/10.24998/maeusabed.653206

Öz

Kolon kanseri, geleneksel tedavi yöntemlerinde kaydedilen ilerlemelere rağmen, dünyada kansere bağlı ölüm nedenleri arasında dördüncü sırada yer almaktadır. Bu nedenle, kolon kanserinin tedavisi için daha etkili yeni tedavi stratejilerine ihtiyaç duyulmaktadır. Bitki özleri kullanılarak sentezlenen gümüş nanopartiküller (AgNP’ler), terapötik uygulamalar göstermiş ve bu durum onları iyi bir kanser önleyici ajan adayı haline getirmiştir. Bu çalışmada, kuşburnu özütü ile sentezlenmiş gümüş nanopartiküllerin insan kolon adenokarsinoma hücre hattı HT29 üzerindeki anti-metastatik ve anti-kanser aktivitesinin değerlendirilmesi amaçlanmıştır. Gümüş nanopartiküllerin biyolojik sentezi, kuşburnu bitkisinin sulu özütü kullanılarak gerçekleştirildi. R-AgNP'ler, UV-görünür spektrofotometre ve taramalı elektron mikroskobu (SEM) gibi çeşitli analitik tekniklerle karakterize edildi. HT29 hücreleri 48 saat süresince çeşitli konsantrasyonlarda R-AgNP'lerle (0-20 µg/mL) muamele edildi. Sentezlenen R-AgNP'lerin insan kolon kanseri hücrelerine karşı sitotoksik aktivitesi MTT assay ile değerlendirildi ve IC50 değerinin 48 saatlik inkübasyon sonucunda 7,89 µg/mL olduğu belirlendi. R-AgNP'lerin anti-metastatik potansiyeli, HT29 hücreleri üzerinde scratch assay kullanılarak çalışıldı. R-AgNP'lerin doza bağımlı olarak hücre hareketliliğinde önemli bir azalmaya neden olduğu belirlendi. Sonuç olarak, bu bulgular biyosentezlenen gümüş nanopartiküllerin insan kolon kanseri tedavisi için umut verici yeni terapötik ajanlar olabileceğini göstermektedir.

Kaynakça

  • Ahmed, S., Ahmad, M., Swami, B.L., Ikram, S.A., 2016. Review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res 7(1), 17–28.
  • Alavi, M., Karimi, N., 2017. Characterization, antibacterial, total antioxidant, scavenging, reducing power and ion chelating activities of green synthesized silver, copper and titanium dioxide nanoparticles using Artemisia haussknechtii leaf extract. Artif Cells Nanomed Biotechnol 12, 1–16.
  • Albrecht, M.A., Evan, C.W., Raston, C.R., 2006. Green chemistry and the health implications of nanoparticles. Green Chem 8, 417-32.
  • Anandalakshmi, K., Venugobal, J., Ramasamy, V., 2016. Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Appl. Nanosci 6, 399–408.
  • Behboodi, S., Baghbani-Arani, F., Abdalan, S., Sadat Shandiz, S.A., 2018. Green Engineered Biomolecule-Capped Silver Nanoparticles Fabricated from Cichorium intybus Extract: In Vitro Assessment on Apoptosis Properties Toward Human Breast Cancer (MCF-7) Cells. Biol Trace Elem Res 187(2), 392-402.
  • Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R.L., Torre, L.A., Jemal, A., 2018. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 68(6), 394-424.
  • Buranasukhon, W., Athikomkulchai, S., Tadtong, S., and Chittasupho, C., 2017. Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract. Pharm Biol 55, 1767–1774.
  • Cheng, B.C., Fua, X.Q., Guo, H., 2016. The genus Rosa and arthritis: overview on pharmacological perspectives. Pharmacol Res 114, 219–234.
  • Chrubasik, J.E., Roufogalis, B.D., Chrubasik, S., 2007. Evidence of effectiveness of herbal antiinflammatory drugs in the treatment of painful osteoarthritis and chronic low back pain. Phytother Res 21(7), 675-83.
  • Clark, A.G., Vignjevic, D.M., 2015. Modes of cancer cell invasion and the role of the microenvironment. Curr Opin Cell Biol 36, 13-22.
  • Daphne, J., Francis, A., Mohanty, R., Ojha, N., Das, N., 2018. Green Synthesis of Antibacterial Silver Nanoparticles using Yeast Isolates and its Characterization. Res J Pharm Technol 11(1), 83–92.
  • Das, S., Das, J., Samadder, A., Bhattacharyya, S.S., Das, D., Khuda- Bukhsh, A.R., 2013. Biosynthesized silver nanoparticles byethanolic extracts of Phytolacca decandra, Gelsemium sempervirens, Hydrastis canadensis and Thuja occidentalis induce differential cytotoxicity through G2/M arrest in A375 cells. Colloids Surf B Biointerfaces 101, 325–336.
  • Dehghanizade, S., Arasteh, J., Mirzaie, A., 2018. Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities. Artif Cells Nanomed Biotechnol 46(1), 160-168.
  • Durai, P., Chinnasamy, A., Gajendran, B., Ramar, M., Pappu, S., Kasivelu, G., Thirunavukkarasu, A., 2014. Synthesis and characterization of silver nanoparticles using crystal compound of sodium para-hydroxybenzoate tetrahydrate isolated from Vitex negundo. L leaves and its apoptotic effect on human colon cancer cell lines. Eur J Med Chem 84, 90-9.
  • Gurunathan, S., Qasim, M., Park, C., Yoo, H., Kim, J.H., Hong, K., 2018. Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116. Int J Mol Sci 19(8). pii: E2269.
  • Hanahan, D., Weinberg, R.A., 2011. Hallmarks of cancer: the next generation. Cell 144, 646-674.
  • Hussain, A., Alajmi, M.F., Khan, M.A., Pervez, S.A., Ahmed, F., Amir, S., Husain, F.M., Khan, M.S., Shaik, G.M., Hassan, I., Khan, R.A., Rehman, M.T., 2019. Biosynthesized Silver Nanoparticle (AgNP) From Pandanus odorifer Leaf Extract Exhibits Anti-metastasis and Anti-biofilm Potentials. Front Microbiol 10, 8.
  • Iravani, S., 2011. Green synthesis of metal nanoparticles using plants. Green Chem 13, 2638.
  • Jiménez, S., Gascón, S., Luquin, A., Laguna, M., Ancin-Azpilicueta, C., Rodríguez-Yoldi, M.J., 2016. Rosa canina Extracts Have Antiproliferative and Antioxidant Effects on Caco-2 Human Colon Cancer. PLoS One 11(7), e0159136.
  • Kavaz, D., Umar, H., Shehu, S., 2018. Synthesis, characterization, antimicrobial and antimetastatic activity of silver nanoparticles synthesized from Ficus ingens leaf. Artif Cells Nanomed Biotechnol 46(sup3), 1193-1203.
  • Kilinc, K., Demir, S., Turan, I., Mentese, A., Orem, A., Sonmez, M., Aliyazicioglu, Y., 2019. Rosa canina Extract has Antiproliferative and Proapoptotic Effects on Human Lung and Prostate Cancer Cells. Nutr Cancer 11, 1-10.
  • Larsen, E., Kharazmi, A., Christensen, L.P., Christensen, S.B., 2003. An antiinflammatory galactolipid from rose hip (Rosa canina) that inhibits chemotaxis of human peripheral blood neutrophils in vitro. J Nat Prod 66(7), 994–5.
  • Liang, C.C., Park, A.Y., Guan, J.L., 2007. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature protocols 2(2), 329.
  • Mármol, I., Sánchez-de-Diego, C., Jiménez-Moreno, N., Ancín-Azpilicueta, C., Rodríguez-Yoldi, M.J., 2017. Therapeutic Applications of Rose Hips from Different Rosa Species. Int J Mol Sci 18(6), pii: E1137.
  • Mata, R., Nakkala, J.R., Sadras, S.R., 2015. Biogenic silver nanoparticles from Abutilon indicum: Their antioxidant, antibacterial and cytotoxic effects in vitro. Colloids Surf. B Biointerfaces 128, 276–286.
  • Mathur, P., Jha, S., Ramteke. S., Jain, N.K., 2017. Pharmaceutical aspects of silver nanoparticles. Artif Cells Nanomed Biotechnol 12, 1–12.
  • Panacek, A., Kvítek, L., Prucek, R., Kolar, M., 2006. Vecerova, R., Pizúrova, N., Sharma, V.K., Nevecna, T., Zboril, R. Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phys Chem B 110, 16248–53.
  • Pulit, J., Banach, M., 2014. Preparation of nanosilver and nanogold based on dog rose aqueous extract. Bioinorg Chem Appl 2014:658935
  • Raveendran, P., Fu, J., Wallen, S.L., 2003. Completely “green” synthesis and stabilization of metal nanoparticles. J Am Chem Soc 125(46), 13940–13941.
  • Sastry, M., Mayya, K.S. Bandyopadhyay, K., 1997. pH Dependent changes in the optical properties of carboxylic acid derivatized silver colloidal particles. Colloids and Surfaces A 127, 221. 27.
  • Sastry, M., Patil, V., Sainkar, S.R., 1998. Electrostatically controlled diffusion of carboxylic acid derivatized silver colloidal particles in thermally evaporated fatty amine films. Journal of Physical Chemistry B 102(8), 1404–1410.
  • Shruti, S., Jain, D. S., Athawale, R. B., Bajaj, A. N., Goel, P., Kamran, Z., et al. 2015. Evaluation of anti-metastatic potential of Cisplatin polymeric nanocarriers on B16F10 melanoma cells. Saudi Pharm J 23, 341–351.
  • Slattery, M.L., 2000. Diet, lifestyle, and colon cancer. Seminars in gastrointestinal disease 11, 142–6.
  • Thakkar, K.N., Mhatre, S.S., Parikh, R.Y., 2010. Biological synthesis of metallic nanoparticles. Nanomedicinee 6, 257-262.
  • Turan, I., Demir. S., Kilinc, K., Yaman, S.O., Misir, S., Kara, H., Genc, B., Mentese. A., Aliyazicioglu, Y., Deger, O., 2018. Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression. J Pharm Anal 8(6), 394-399.
  • Venugopal, K., Rather, H. A , Rajagopal, K., Shanthi, M.P., Sheriff. K., Illiyas, M., Rather, R.A., Manikandan, E., Uvarajan, S., Bhaskar, M., Maaza, M., 2017. The impact of anticancer activity upon Beta vulgaris extract mediated biosynthesized silver nanoparticles (Ag-NPs) against human breast (MCF-7), lung (A549) and pharynx (Hep-2) cancer cell lines. J Photochem Photobiol B 167, 282-289.
  • Yesilot, S., Aydin, C.A., 2019. Silver nanoparticles; a new hope in cancer therapy?. Eastern J Med 24(1), 111-116

Biosynthesis of silver nanoparticles using Rosa canina extract and its anti-cancer and anti-metastatic activity on human colon adenocarcinoma cell line HT29

Yıl 2019, Cilt: 7 Sayı: 2, 124 - 131, 30.12.2019
https://doi.org/10.24998/maeusabed.653206

Öz

Despite progress in conventional treatment methods for colon cancer, it remains the fourth leading cause of cancer-related deaths in the world. Therefore, more effective new treatment strategies for colon cancer are needed. Silver nanoparticles (AgNPs) synthesized using plant extracts have shown therapeutic applications and make it to be a good anti-cancer candidates. The aim of this study was to evaluate the anti-metastatic and anti-cancer activity of biosynthesized silver nanoparticles from Rosa canina extract on the human colon adenocarcinoma cell line HT29. The biosynthesis of AgNPs was carried out using Rosa canina extract. R-AgNPs were characterized by techniques such as UV-vis spectrophotometer and scaining electron microscopy (SEM). HT29 cells were incubated with different concentrations of AgNPs (0-20 µg/mL) for 48 h. The cytotoxic activity of the synthesized R-AgNPs against human colon adenocarcinoma cells HT29 was investigated by MTT assay and the IC50 value were found to be 7,89 µg/mL at 48 h incubation. Anti-metastatic potential of R-AgNPs were determined on HT29 cells using scratch assay. R-AgNPs induced a significant decrease of cell motility in dose-dependent manner. In conlusion, these findings suggest that the biosynthesized AgNPs may be promising new therapeutic agents for the treatment of human colon cancer.

Kaynakça

  • Ahmed, S., Ahmad, M., Swami, B.L., Ikram, S.A., 2016. Review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res 7(1), 17–28.
  • Alavi, M., Karimi, N., 2017. Characterization, antibacterial, total antioxidant, scavenging, reducing power and ion chelating activities of green synthesized silver, copper and titanium dioxide nanoparticles using Artemisia haussknechtii leaf extract. Artif Cells Nanomed Biotechnol 12, 1–16.
  • Albrecht, M.A., Evan, C.W., Raston, C.R., 2006. Green chemistry and the health implications of nanoparticles. Green Chem 8, 417-32.
  • Anandalakshmi, K., Venugobal, J., Ramasamy, V., 2016. Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Appl. Nanosci 6, 399–408.
  • Behboodi, S., Baghbani-Arani, F., Abdalan, S., Sadat Shandiz, S.A., 2018. Green Engineered Biomolecule-Capped Silver Nanoparticles Fabricated from Cichorium intybus Extract: In Vitro Assessment on Apoptosis Properties Toward Human Breast Cancer (MCF-7) Cells. Biol Trace Elem Res 187(2), 392-402.
  • Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R.L., Torre, L.A., Jemal, A., 2018. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 68(6), 394-424.
  • Buranasukhon, W., Athikomkulchai, S., Tadtong, S., and Chittasupho, C., 2017. Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract. Pharm Biol 55, 1767–1774.
  • Cheng, B.C., Fua, X.Q., Guo, H., 2016. The genus Rosa and arthritis: overview on pharmacological perspectives. Pharmacol Res 114, 219–234.
  • Chrubasik, J.E., Roufogalis, B.D., Chrubasik, S., 2007. Evidence of effectiveness of herbal antiinflammatory drugs in the treatment of painful osteoarthritis and chronic low back pain. Phytother Res 21(7), 675-83.
  • Clark, A.G., Vignjevic, D.M., 2015. Modes of cancer cell invasion and the role of the microenvironment. Curr Opin Cell Biol 36, 13-22.
  • Daphne, J., Francis, A., Mohanty, R., Ojha, N., Das, N., 2018. Green Synthesis of Antibacterial Silver Nanoparticles using Yeast Isolates and its Characterization. Res J Pharm Technol 11(1), 83–92.
  • Das, S., Das, J., Samadder, A., Bhattacharyya, S.S., Das, D., Khuda- Bukhsh, A.R., 2013. Biosynthesized silver nanoparticles byethanolic extracts of Phytolacca decandra, Gelsemium sempervirens, Hydrastis canadensis and Thuja occidentalis induce differential cytotoxicity through G2/M arrest in A375 cells. Colloids Surf B Biointerfaces 101, 325–336.
  • Dehghanizade, S., Arasteh, J., Mirzaie, A., 2018. Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities. Artif Cells Nanomed Biotechnol 46(1), 160-168.
  • Durai, P., Chinnasamy, A., Gajendran, B., Ramar, M., Pappu, S., Kasivelu, G., Thirunavukkarasu, A., 2014. Synthesis and characterization of silver nanoparticles using crystal compound of sodium para-hydroxybenzoate tetrahydrate isolated from Vitex negundo. L leaves and its apoptotic effect on human colon cancer cell lines. Eur J Med Chem 84, 90-9.
  • Gurunathan, S., Qasim, M., Park, C., Yoo, H., Kim, J.H., Hong, K., 2018. Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116. Int J Mol Sci 19(8). pii: E2269.
  • Hanahan, D., Weinberg, R.A., 2011. Hallmarks of cancer: the next generation. Cell 144, 646-674.
  • Hussain, A., Alajmi, M.F., Khan, M.A., Pervez, S.A., Ahmed, F., Amir, S., Husain, F.M., Khan, M.S., Shaik, G.M., Hassan, I., Khan, R.A., Rehman, M.T., 2019. Biosynthesized Silver Nanoparticle (AgNP) From Pandanus odorifer Leaf Extract Exhibits Anti-metastasis and Anti-biofilm Potentials. Front Microbiol 10, 8.
  • Iravani, S., 2011. Green synthesis of metal nanoparticles using plants. Green Chem 13, 2638.
  • Jiménez, S., Gascón, S., Luquin, A., Laguna, M., Ancin-Azpilicueta, C., Rodríguez-Yoldi, M.J., 2016. Rosa canina Extracts Have Antiproliferative and Antioxidant Effects on Caco-2 Human Colon Cancer. PLoS One 11(7), e0159136.
  • Kavaz, D., Umar, H., Shehu, S., 2018. Synthesis, characterization, antimicrobial and antimetastatic activity of silver nanoparticles synthesized from Ficus ingens leaf. Artif Cells Nanomed Biotechnol 46(sup3), 1193-1203.
  • Kilinc, K., Demir, S., Turan, I., Mentese, A., Orem, A., Sonmez, M., Aliyazicioglu, Y., 2019. Rosa canina Extract has Antiproliferative and Proapoptotic Effects on Human Lung and Prostate Cancer Cells. Nutr Cancer 11, 1-10.
  • Larsen, E., Kharazmi, A., Christensen, L.P., Christensen, S.B., 2003. An antiinflammatory galactolipid from rose hip (Rosa canina) that inhibits chemotaxis of human peripheral blood neutrophils in vitro. J Nat Prod 66(7), 994–5.
  • Liang, C.C., Park, A.Y., Guan, J.L., 2007. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature protocols 2(2), 329.
  • Mármol, I., Sánchez-de-Diego, C., Jiménez-Moreno, N., Ancín-Azpilicueta, C., Rodríguez-Yoldi, M.J., 2017. Therapeutic Applications of Rose Hips from Different Rosa Species. Int J Mol Sci 18(6), pii: E1137.
  • Mata, R., Nakkala, J.R., Sadras, S.R., 2015. Biogenic silver nanoparticles from Abutilon indicum: Their antioxidant, antibacterial and cytotoxic effects in vitro. Colloids Surf. B Biointerfaces 128, 276–286.
  • Mathur, P., Jha, S., Ramteke. S., Jain, N.K., 2017. Pharmaceutical aspects of silver nanoparticles. Artif Cells Nanomed Biotechnol 12, 1–12.
  • Panacek, A., Kvítek, L., Prucek, R., Kolar, M., 2006. Vecerova, R., Pizúrova, N., Sharma, V.K., Nevecna, T., Zboril, R. Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phys Chem B 110, 16248–53.
  • Pulit, J., Banach, M., 2014. Preparation of nanosilver and nanogold based on dog rose aqueous extract. Bioinorg Chem Appl 2014:658935
  • Raveendran, P., Fu, J., Wallen, S.L., 2003. Completely “green” synthesis and stabilization of metal nanoparticles. J Am Chem Soc 125(46), 13940–13941.
  • Sastry, M., Mayya, K.S. Bandyopadhyay, K., 1997. pH Dependent changes in the optical properties of carboxylic acid derivatized silver colloidal particles. Colloids and Surfaces A 127, 221. 27.
  • Sastry, M., Patil, V., Sainkar, S.R., 1998. Electrostatically controlled diffusion of carboxylic acid derivatized silver colloidal particles in thermally evaporated fatty amine films. Journal of Physical Chemistry B 102(8), 1404–1410.
  • Shruti, S., Jain, D. S., Athawale, R. B., Bajaj, A. N., Goel, P., Kamran, Z., et al. 2015. Evaluation of anti-metastatic potential of Cisplatin polymeric nanocarriers on B16F10 melanoma cells. Saudi Pharm J 23, 341–351.
  • Slattery, M.L., 2000. Diet, lifestyle, and colon cancer. Seminars in gastrointestinal disease 11, 142–6.
  • Thakkar, K.N., Mhatre, S.S., Parikh, R.Y., 2010. Biological synthesis of metallic nanoparticles. Nanomedicinee 6, 257-262.
  • Turan, I., Demir. S., Kilinc, K., Yaman, S.O., Misir, S., Kara, H., Genc, B., Mentese. A., Aliyazicioglu, Y., Deger, O., 2018. Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression. J Pharm Anal 8(6), 394-399.
  • Venugopal, K., Rather, H. A , Rajagopal, K., Shanthi, M.P., Sheriff. K., Illiyas, M., Rather, R.A., Manikandan, E., Uvarajan, S., Bhaskar, M., Maaza, M., 2017. The impact of anticancer activity upon Beta vulgaris extract mediated biosynthesized silver nanoparticles (Ag-NPs) against human breast (MCF-7), lung (A549) and pharynx (Hep-2) cancer cell lines. J Photochem Photobiol B 167, 282-289.
  • Yesilot, S., Aydin, C.A., 2019. Silver nanoparticles; a new hope in cancer therapy?. Eastern J Med 24(1), 111-116
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makalesi
Yazarlar

Çiğdem Aydın Acar 0000-0002-1311-2314

Süray Pehlivanoğlu 0000-0001-7422-2974

Yayımlanma Tarihi 30 Aralık 2019
Gönderilme Tarihi 29 Kasım 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 7 Sayı: 2

Kaynak Göster

APA Aydın Acar, Ç., & Pehlivanoğlu, S. (2019). Biosynthesis of silver nanoparticles using Rosa canina extract and its anti-cancer and anti-metastatic activity on human colon adenocarcinoma cell line HT29. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 7(2), 124-131. https://doi.org/10.24998/maeusabed.653206
AMA Aydın Acar Ç, Pehlivanoğlu S. Biosynthesis of silver nanoparticles using Rosa canina extract and its anti-cancer and anti-metastatic activity on human colon adenocarcinoma cell line HT29. Mehmet Akif Ersoy University Journal of Health Sciences Institute. Aralık 2019;7(2):124-131. doi:10.24998/maeusabed.653206
Chicago Aydın Acar, Çiğdem, ve Süray Pehlivanoğlu. “Biosynthesis of Silver Nanoparticles Using Rosa Canina Extract and Its Anti-Cancer and Anti-Metastatic Activity on Human Colon Adenocarcinoma Cell Line HT29”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 7, sy. 2 (Aralık 2019): 124-31. https://doi.org/10.24998/maeusabed.653206.
EndNote Aydın Acar Ç, Pehlivanoğlu S (01 Aralık 2019) Biosynthesis of silver nanoparticles using Rosa canina extract and its anti-cancer and anti-metastatic activity on human colon adenocarcinoma cell line HT29. Mehmet Akif Ersoy University Journal of Health Sciences Institute 7 2 124–131.
IEEE Ç. Aydın Acar ve S. Pehlivanoğlu, “Biosynthesis of silver nanoparticles using Rosa canina extract and its anti-cancer and anti-metastatic activity on human colon adenocarcinoma cell line HT29”, Mehmet Akif Ersoy University Journal of Health Sciences Institute, c. 7, sy. 2, ss. 124–131, 2019, doi: 10.24998/maeusabed.653206.
ISNAD Aydın Acar, Çiğdem - Pehlivanoğlu, Süray. “Biosynthesis of Silver Nanoparticles Using Rosa Canina Extract and Its Anti-Cancer and Anti-Metastatic Activity on Human Colon Adenocarcinoma Cell Line HT29”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 7/2 (Aralık 2019), 124-131. https://doi.org/10.24998/maeusabed.653206.
JAMA Aydın Acar Ç, Pehlivanoğlu S. Biosynthesis of silver nanoparticles using Rosa canina extract and its anti-cancer and anti-metastatic activity on human colon adenocarcinoma cell line HT29. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2019;7:124–131.
MLA Aydın Acar, Çiğdem ve Süray Pehlivanoğlu. “Biosynthesis of Silver Nanoparticles Using Rosa Canina Extract and Its Anti-Cancer and Anti-Metastatic Activity on Human Colon Adenocarcinoma Cell Line HT29”. Mehmet Akif Ersoy University Journal of Health Sciences Institute, c. 7, sy. 2, 2019, ss. 124-31, doi:10.24998/maeusabed.653206.
Vancouver Aydın Acar Ç, Pehlivanoğlu S. Biosynthesis of silver nanoparticles using Rosa canina extract and its anti-cancer and anti-metastatic activity on human colon adenocarcinoma cell line HT29. Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2019;7(2):124-31.