Öz
Mantarlar, binlerce yıldır besin ve tıbbi değerleri nedeniyle yaygın olarak kullanılmaktadır. Bu çalışma iki yenilebilir mantar olan Armillaria mellea (Vahl) P.Kumm ve Macrolepiota procera (Scop.) antioksidan ve antimikrobiyal potansiyelinin belirlenmesi için tasarlanmıştır Antioksidan aktivitesi DPHH serbest radikal süpürme yöntemi ile tespit edildi. M.procera methanol ekstresi A.mellea methanol ekstresinden daha güçlü serbest radikal süpürücü aktivitesine sahiptir (IC50: 0.191, 1.19 mg/mL). Toplam fenol, β-karoten ve likopen gibi antioksidan özelliklere sahip bileşenlerin konsantrasyonu spektrofotometrik yöntemlerle belirlendi. Son olarak, antimikrobiyal aktivitesi 14 mikroorganizma üzerinde denenmiş olup agar difüzyon yöntemi ile kullanılmıştır. A. mellea metanol ekstraktı Klebsiella pneumaniae ATCC 13883, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923 mikroorganizmlarına karşı 10 ± 1 mm inhibisyon zon çapı oluşturdu. M.procera metanol ekstresi ise Enterococcus faecalis ATCC 29212, Klebsiella pneumaniae ATCC 13883 mikroorganizmlarına karşı 9 ± 1 mm inhibisyon zon çapı oluşturdu Bu araştırma, iki yenilebilir mantarın doğal antioksidanlar olarak potansiyele sahip olduğunu göstermiştir.
Anahtar Kelimeler
Antimikrobiyal aktivite mantar Macrolepiota procera Antioksidan aktivite Armillaria mellea
Kaynakça
- Arteaga,V.,Lamas, A.,Regal,P.,Vazquez,B.,Miranda, J.M.,Cepeda, A.,Franco,C.M.(2019).Antimicrobial activity of apitoxin from Apis mellifera in Salmonella enterica strains isolated from poultry and its effects on motility , biofilm formation and gene expresiion.Microbial Pathognesis, 137:103771.
- Akata, I.,Ergönül, B. and Kalyoncu, F. (2012).Chemical compositions and antioxidant activities of 16 wild edible mushroom species grown in Anatolia. International Journal of Pharmacology, 8(2):134-138.
- Akata, I. and Zengin, G. (2019). Enzyme inhibitory and antioxidant properties of six mushroom species from the Agaricaceae family. South African Journal of Botany, 120: 95–99.
- Arora, S., Shivhare, U.S., Ahmed, J. & Raghavan, G.S.V. (2003). Drying kinetics of Agaricus bisporus and Pleurotus florida mushrooms. American Society of Agricultural Engineers, 46: 721–724.
- Braca, A., De Tommasi, N., Di Bari, L., Pizza, C., Politi, M., Morelli, I. (2001). Antioxidant principles from bauhinia tarapotensis. Journal of Natural Products ,64(7):892–895.
- Barros, L., Baptista, P., Ferreira, I.C.F.R. (2007). Effect of Lactarius piperatus fruiting body maturity stage on antioxidant activity measured by several biochemical assays. Food and Chemical Toxicology, 45: 1731-1737.
- Becker, E.M., Nissen, L.R. and Skibsted, L.H. (2004). Antioxidant Evaluation Protocols: Food Quality or Health Effects. European Food Research and Technology,219: 561-571.
- Bobek, P., Galbavy, S. (2001). Effect of pleuran (b-glucan from Pleurotus ostreatus) on the antioxidant status of the organism and on dimethylhydrazine-induced precancerous lesions in rat colon. British Journal of Biomedical Science, 58:164-171.
- Borchers, A.T., Krishnamurthy, A., Keen, C.L., Meyers, F.J., Gershwin, M.E. (2008). The immunobiology of mushrooms. Experimental Biology and Medicine, 233:259-276.
- Droge, W. (2002).Free radicals in the physiological control of cellfunction, Physiological Reviews, 82: 47–95.
- Dong, Q., Jia, L. M .& Fang, J. N .(2006). A beta-D-glucan isolated from the fruiting bodies of Hericium erinaceus and its aqueous conformation. Carbohydrate Research, 341(6): 791–795.
- Falandysz, J., Kunito, T., Kubota, R., Gucia, M., Mazur, A., Falandysz, J.J., Tanabe, S. (2008). Some mineral constituents of Parasol Mushroom (Macrolepiota procera). Journal of Environmental Science and Health, 43(2): 187-192.
- Fernandes, Â., Barreira, J.C.M., Antonio, A.L., Oliveira, M.B.P.P., Martins, A., Ferreira, I.C.F.R. (2014). Effects of gamma irradition on chemical composition and antioxidant potential of processed samples of the wild mushroom Macrolepiota procera. Food Chemistry, 149: 91-98.
- Ferreira, I.C.F.R., Barros, L., Abreu, R.M. (2009). Antioxidants in wild mushrooms. Current Medical Chemistry, 16 (12): 1543-1560.
- Ji, Z., Tang, Q., Zhang, J., Yang, Y., Jia ,W. & Pan, Y. (2007). Immunomodulation of RAW264.7 macrophages by GLIS, a proteopolysaccharide from Ganoderma lucidum. Journal of Ethnopharmacology, 112(3): 445–450.
- Kalyoncu F, Oskay M, Saglam H, Erdogan TF, Tamer AU (2010). Antimicrobial and Antioxidant Activities of Mycelia of 10 Wild Mushroom Species. Journal of Medicinal Food 13 (2): 415-9.
- Kosanic, M., Rankovic, B., Rancic, A., Stanojkovij, T. (2016). Evaluation of metal concentration and antioxidant, antimicrobial, and anticancer potentials of two edible mushrooms Lactarius deliciosus and Macrolepiota procera. Journal of Food and Frug Analysis, 24 :477 -84.
- Kumari, B., Atri, N.S. (2014). Nutritional and nutraceutical potential of wild edible macrolepiotoid mushrooms of North India. International Journal of Pharmacy and Pharmaceutical Sciences ,6(2): 200-204.
- Lai, M.N.,Ng, L.T. (2013). Antioxidant and antiedema properties of solid-state cultured honey mushroom, Armillaria mellea (higher Basidiomycetes), extracts and their polysaccharide and polyphenol contents. International Journal of Medicinal Mushrooms, 15:1-8.
- Lindshield, B.L., Canene-Adams, K., Erdman, J.J.W. (2007). Lycopenoids. Journal of Nutrition, 122 (11): 2161-2166.
- Lung, M.Y. and Chang, Y.C. (2011). Antioxidant Properties of the Edible Basidiomycete Armillaria mellea in Submerged Cultures. International Journal of Molecular Sciences, 12: 6367-6384.
- Miura, Y., Kondo, K., Saito, T., Shimada, H., Fraser, P. and Misawa, N. (1998).Production of the carotenoids lycopene, carotene, and astaxanhin in the food yeast candida utilis. Applıed Environmental Microbiology, 64: 1226-1229.
- Muszyńska, B., Grzywacz-Kisielewska, A., Kała, K., Gdula-Argasińska, J. (2017). Antiinflammatory properties of edible mushrooms. Food Chemistry, 243:373–381.
- Nagata, M. and Yamashita, I. (1992). Simplemethod for simultaneous determination of chlorophyll and carotenoids in tomato fruit. Nippon Shokuhin Kogyo Gakkaish, 39: 925–928.
- Nowacka, N., Nowak, R., Drozd, M., Olech, M., Los, R. &Malm, A. (2015). Antibacterial, antiradical potential and phenolic compounds of thirty-one polish mushrooms. Public Library Science, 10(10):140-355.
- Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., Bitto, A. (2017).Oxidative stress: harms and Benefits for human health. Oxidative Medicine and Cellular Longevity 84:1–13.
- Popescu, M.L., Costea, T.,Nencu, I., Dutu, L.E. (2016). Polyphenols contents and antıoxıdant actıvıty of some Romanıan wıld mushrooms. Farmacıa, 64: 2.
- Sarikurkcu, C.,Tepe, B.,Kocak, M.S., Uren, M.C .(2015). Metal concentration and antioxidant activity of edible mushrooms from Turkey. Food Chemistry, 175: 549-555.
- Siddhuraju, P. and Becker, K. (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. Journal of Aricultural and Food Chemistry, 51(8):2144-2155.
- Son, S.M. (2012). Reactive oxygen and nitrogen species in pathogenesis of vascular complication of diabetes. Diabetes and Metabolısm, 36: 190–198.
- Sullivan, R., Smith, J.E., Rowan, N.J. (2006). Medicinal mushrooms and cancer therapy: translating a traditional practice into Western medicine. Perspectives in Biology and Medicine, 49:159-170.
- Turpaev, K.T. (2002). Reactive oxygen species and regulation of gene expression. Biochemistry (Moscow), 67(3):281-92.
- Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M., Telser, J (2007). Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology, 39: 44-84.
- Willis,K.J.(2018).State of the World’s Fungi 2018.Royal Botanic Gardens, 80:92-93.
- Yamac, M., Bilgili, F. (2006). Antimicrobial activities of fruit bodies and/or mycelia cultures of some mushroom isolates. Pharmaceutical Biology, 44: 660-667.
- Yang, J.S., Chen, Y.W., Feng, X.Z., Yu, .D.Q., He, C.H., Zheng, Q.T., Yang, J., Liang, X.T. (1989). Isolation and structure elucidation of Armillaricin. Planta Medica, 55: 564–565.
- Yang, L.H., Huang, Q.R., Feng, P.Y., Jiang, H., Cai, D.H., Liu, J. (2007).Isolation and identification of polysaccharides from Armillaria mellea and their effects on scavenging oxygen free radicals. Food Science, 28: 309–313.
- Yaping, Z., Suping, Q., Wenli, Y., Zheng, X., Hong, S., Side, Y., Dapu, W. (2002). Antioxidant activity of lycopene extracted from tomato paste towards tri chloro methyl peroxyl radical CCl3O2. Food Chemistry, 77: 209-212.
- Young, I. and Woodside, J. (2001).Antioxidants in health anddisease. Journal of Clinical Pathology, 54: 176–186.
Öz
Mushrooms have been used extensively, owing to their nutritional and medicinal value, for thousands of years. This study designed for the determine of antioxidant and antimicrobial potential of two edible mushrooms Armillaria mellea (Vahl) P.Kumm. and Macrolepiota procera (Scop.) Singer. Antioxidant activity was detected method by DPHH free radical scavenging. M.procera extract had more potent free radical scavenging activity than A.mellea extract (IC50: 0.191, 1.19 mg/mL). The concent of the components with antioxidant properties, such as total phenols,β-caratone and lycopene were determined by spectrophotometric methods. Finally, the antimicrobial potential was determined with a agar well diffusion method on 14 microorganisms. A. mellea methanol extract formed against to Klebsiella pneumaniae ATCC 13883, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923,10±1 mm inhibition zone diameter. M.procera methanol extract formed against to Enterococcus faecalis ATCC 29212, Klebsiella pneumaniae ATCC 13883, 9±1 mm inhibition zone diameter. This research has shown that these two edible mushrooms have potential as natural antioxidants.
Anahtar Kelimeler
Armillea mellea Macrolepiota procera mushroom Antimicrobial activity Antioxidant activity
Kaynakça
- Arteaga,V.,Lamas, A.,Regal,P.,Vazquez,B.,Miranda, J.M.,Cepeda, A.,Franco,C.M.(2019).Antimicrobial activity of apitoxin from Apis mellifera in Salmonella enterica strains isolated from poultry and its effects on motility , biofilm formation and gene expresiion.Microbial Pathognesis, 137:103771.
- Akata, I.,Ergönül, B. and Kalyoncu, F. (2012).Chemical compositions and antioxidant activities of 16 wild edible mushroom species grown in Anatolia. International Journal of Pharmacology, 8(2):134-138.
- Akata, I. and Zengin, G. (2019). Enzyme inhibitory and antioxidant properties of six mushroom species from the Agaricaceae family. South African Journal of Botany, 120: 95–99.
- Arora, S., Shivhare, U.S., Ahmed, J. & Raghavan, G.S.V. (2003). Drying kinetics of Agaricus bisporus and Pleurotus florida mushrooms. American Society of Agricultural Engineers, 46: 721–724.
- Braca, A., De Tommasi, N., Di Bari, L., Pizza, C., Politi, M., Morelli, I. (2001). Antioxidant principles from bauhinia tarapotensis. Journal of Natural Products ,64(7):892–895.
- Barros, L., Baptista, P., Ferreira, I.C.F.R. (2007). Effect of Lactarius piperatus fruiting body maturity stage on antioxidant activity measured by several biochemical assays. Food and Chemical Toxicology, 45: 1731-1737.
- Becker, E.M., Nissen, L.R. and Skibsted, L.H. (2004). Antioxidant Evaluation Protocols: Food Quality or Health Effects. European Food Research and Technology,219: 561-571.
- Bobek, P., Galbavy, S. (2001). Effect of pleuran (b-glucan from Pleurotus ostreatus) on the antioxidant status of the organism and on dimethylhydrazine-induced precancerous lesions in rat colon. British Journal of Biomedical Science, 58:164-171.
- Borchers, A.T., Krishnamurthy, A., Keen, C.L., Meyers, F.J., Gershwin, M.E. (2008). The immunobiology of mushrooms. Experimental Biology and Medicine, 233:259-276.
- Droge, W. (2002).Free radicals in the physiological control of cellfunction, Physiological Reviews, 82: 47–95.
- Dong, Q., Jia, L. M .& Fang, J. N .(2006). A beta-D-glucan isolated from the fruiting bodies of Hericium erinaceus and its aqueous conformation. Carbohydrate Research, 341(6): 791–795.
- Falandysz, J., Kunito, T., Kubota, R., Gucia, M., Mazur, A., Falandysz, J.J., Tanabe, S. (2008). Some mineral constituents of Parasol Mushroom (Macrolepiota procera). Journal of Environmental Science and Health, 43(2): 187-192.
- Fernandes, Â., Barreira, J.C.M., Antonio, A.L., Oliveira, M.B.P.P., Martins, A., Ferreira, I.C.F.R. (2014). Effects of gamma irradition on chemical composition and antioxidant potential of processed samples of the wild mushroom Macrolepiota procera. Food Chemistry, 149: 91-98.
- Ferreira, I.C.F.R., Barros, L., Abreu, R.M. (2009). Antioxidants in wild mushrooms. Current Medical Chemistry, 16 (12): 1543-1560.
- Ji, Z., Tang, Q., Zhang, J., Yang, Y., Jia ,W. & Pan, Y. (2007). Immunomodulation of RAW264.7 macrophages by GLIS, a proteopolysaccharide from Ganoderma lucidum. Journal of Ethnopharmacology, 112(3): 445–450.
- Kalyoncu F, Oskay M, Saglam H, Erdogan TF, Tamer AU (2010). Antimicrobial and Antioxidant Activities of Mycelia of 10 Wild Mushroom Species. Journal of Medicinal Food 13 (2): 415-9.
- Kosanic, M., Rankovic, B., Rancic, A., Stanojkovij, T. (2016). Evaluation of metal concentration and antioxidant, antimicrobial, and anticancer potentials of two edible mushrooms Lactarius deliciosus and Macrolepiota procera. Journal of Food and Frug Analysis, 24 :477 -84.
- Kumari, B., Atri, N.S. (2014). Nutritional and nutraceutical potential of wild edible macrolepiotoid mushrooms of North India. International Journal of Pharmacy and Pharmaceutical Sciences ,6(2): 200-204.
- Lai, M.N.,Ng, L.T. (2013). Antioxidant and antiedema properties of solid-state cultured honey mushroom, Armillaria mellea (higher Basidiomycetes), extracts and their polysaccharide and polyphenol contents. International Journal of Medicinal Mushrooms, 15:1-8.
- Lindshield, B.L., Canene-Adams, K., Erdman, J.J.W. (2007). Lycopenoids. Journal of Nutrition, 122 (11): 2161-2166.
- Lung, M.Y. and Chang, Y.C. (2011). Antioxidant Properties of the Edible Basidiomycete Armillaria mellea in Submerged Cultures. International Journal of Molecular Sciences, 12: 6367-6384.
- Miura, Y., Kondo, K., Saito, T., Shimada, H., Fraser, P. and Misawa, N. (1998).Production of the carotenoids lycopene, carotene, and astaxanhin in the food yeast candida utilis. Applıed Environmental Microbiology, 64: 1226-1229.
- Muszyńska, B., Grzywacz-Kisielewska, A., Kała, K., Gdula-Argasińska, J. (2017). Antiinflammatory properties of edible mushrooms. Food Chemistry, 243:373–381.
- Nagata, M. and Yamashita, I. (1992). Simplemethod for simultaneous determination of chlorophyll and carotenoids in tomato fruit. Nippon Shokuhin Kogyo Gakkaish, 39: 925–928.
- Nowacka, N., Nowak, R., Drozd, M., Olech, M., Los, R. &Malm, A. (2015). Antibacterial, antiradical potential and phenolic compounds of thirty-one polish mushrooms. Public Library Science, 10(10):140-355.
- Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., Bitto, A. (2017).Oxidative stress: harms and Benefits for human health. Oxidative Medicine and Cellular Longevity 84:1–13.
- Popescu, M.L., Costea, T.,Nencu, I., Dutu, L.E. (2016). Polyphenols contents and antıoxıdant actıvıty of some Romanıan wıld mushrooms. Farmacıa, 64: 2.
- Sarikurkcu, C.,Tepe, B.,Kocak, M.S., Uren, M.C .(2015). Metal concentration and antioxidant activity of edible mushrooms from Turkey. Food Chemistry, 175: 549-555.
- Siddhuraju, P. and Becker, K. (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. Journal of Aricultural and Food Chemistry, 51(8):2144-2155.
- Son, S.M. (2012). Reactive oxygen and nitrogen species in pathogenesis of vascular complication of diabetes. Diabetes and Metabolısm, 36: 190–198.
- Sullivan, R., Smith, J.E., Rowan, N.J. (2006). Medicinal mushrooms and cancer therapy: translating a traditional practice into Western medicine. Perspectives in Biology and Medicine, 49:159-170.
- Turpaev, K.T. (2002). Reactive oxygen species and regulation of gene expression. Biochemistry (Moscow), 67(3):281-92.
- Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M., Telser, J (2007). Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology, 39: 44-84.
- Willis,K.J.(2018).State of the World’s Fungi 2018.Royal Botanic Gardens, 80:92-93.
- Yamac, M., Bilgili, F. (2006). Antimicrobial activities of fruit bodies and/or mycelia cultures of some mushroom isolates. Pharmaceutical Biology, 44: 660-667.
- Yang, J.S., Chen, Y.W., Feng, X.Z., Yu, .D.Q., He, C.H., Zheng, Q.T., Yang, J., Liang, X.T. (1989). Isolation and structure elucidation of Armillaricin. Planta Medica, 55: 564–565.
- Yang, L.H., Huang, Q.R., Feng, P.Y., Jiang, H., Cai, D.H., Liu, J. (2007).Isolation and identification of polysaccharides from Armillaria mellea and their effects on scavenging oxygen free radicals. Food Science, 28: 309–313.
- Yaping, Z., Suping, Q., Wenli, Y., Zheng, X., Hong, S., Side, Y., Dapu, W. (2002). Antioxidant activity of lycopene extracted from tomato paste towards tri chloro methyl peroxyl radical CCl3O2. Food Chemistry, 77: 209-212.
- Young, I. and Woodside, J. (2001).Antioxidants in health anddisease. Journal of Clinical Pathology, 54: 176–186.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | ARAŞTIRMA MAKALESİ |
| Yazarlar | |
| Yayımlanma Tarihi | 28 Ekim 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 11 Sayı: 2 |
Uluslararası Hakemli Dergi
Dergimiz, herhangi bir başvuru veya yayımlama ücreti almamaktadır
