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Türkiye'den Melanoleuca cinereifolia Türünün Moleküler ve Morfolojik Teşhisi

Yıl 2022, Cilt: 13 Sayı: 2, 134 - 140, 31.10.2022

Ayşenur Kalmer Ayten Dizkırıcı İsmail Acar

Öz

Bu çalışmada Melanoleuca cinereifolia, Türkiye'nin Diyarbakır ilinden ilk kez rapor edilmiş, makro/mikro-morfolojik karakterleri ile nükleer ribozomal Internal Transcribed Spacer (nrITS) bölgesinin DNA dizileri baz alınarak tanımlanmıştır. Türün makro ve mikro morfolojik tanımı, doğal ortamdaki taze bazidyomların fotoğrafları ve mikroskobik yapılarının çizimleri ile birlikte verilmiştir. Bu tür esas olarak kısa saplı, grimsi kahverengi şapka, gri lamelli ve şişe biçiminde sistityumlu bazidiyom ile karakterize edilir. Filogramda, M. cinereifolia, Melanoleuca alt cinsindeki akrabaları ile birlikte kümelenmiştir. Türler ve yakın akrabaları arasındaki morfolojik ve moleküler benzerlikler ve farklılıklar ayrıntılı olarak tartışılmıştır.

Anahtar Kelimeler

DNA ITS Moleküler taksonomi Agaricales

Kaynakça

  • Acar, I., Dizkirici Tekpınar, A., Kalmer, A., Uzun, Y. (2017). Phylogenetic relationships and taxonomical positions of two new records Melanoleuca species from Hakkâri province, Turkey. Biodicon, 10(3): 85-93.
  • Antonín, V., Ďuriška, O., Gafforov, Y., Jančovičová, S., Para, R., Tomšovský, M. (2017). Molecular phylogenetics and taxonomy in Melanoleuca exscissa group, (Tricholomataceae, Basidiomycota) and the description of M. griseobrunnea sp. nov. Plant Syst Evol, 303:1181-1198. DOI 10.1007/s00606-017-1430-y
  • Antonín, V., Ďuriška, O., Jančovičová, S., Para, R., Kudláček, T., Tomšovský, M. (2021). Multilocus phylogeny and taxonomy of European Melanoleuca subgenus Melanoleuca. Mycologia, DOI: 10.1080/00275514.2021.1966246
  • Bahadori, M.B., Sarikurkcu, C., Yalcin, O., Cengiz, M., Gungor, H. (2019). Metal concentration, phenolics profiling, and antioxidant activity of two wild edible Melanoleuca mushrooms (M. cognata and M. stridula). Microchemical Journal, 150:104172. DOI: 10.1016/j.microc.2019.104172
  • Breitenbach, J. and Kränzlin, F. (1991). Fungi of Switzerland. Verlag Mykologia. 3rd ed. Lucerne, Switzerland.
  • Boekhout, T. (1999). Melanoleuca, in: C. Bas et al. (eds). Flora Agaricina Neerlandica 4.A.A. Balkema, Rotterdam, pp. 153-165.
  • Bon, M. (1991). Flore mycologiqued’Europe, 2 – Les Tricholomesetressemblants. Revue de Mycologie Mémóire Hors-Série, 2, 163.
  • Buczacki, S. (2012). Collins Fungi Guide: The Most Complete Field Guide to the Mushrooms and Toadstools of Britain & Ireland. Harper Collins Publishers, London, UK.
  • Doyle, J.J., Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19,11-15.
  • Ďuriška, O., Antonín, V., Para, R., Tomšovsky, M., Jančovičova, S. (2017). Taxonomy, ecology and distribution of Melanoleuca strictipes (Basidiomycota, Agaricales) in Europe. Czech Mycology, 69(1): 15-30.
  • Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W., Gascuel, O. (2010). New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Systematic Biology, 59(3):307-21. https://doi.org/10.1093/sysbio/syq010
  • Hoang, D.T., Chernomor, O., von Haeseler, A., Minh, B.Q., Vinh, L.S. (2018) UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35:518–522. https://doi.org/10.1093/molbev/msx281
  • Kalmer, A., Acar, I., Dizkirici Tekpınar, A. (2018). Phylogeny of Some Melanoleuca Species (Fungi: Basidiomycota) in Turkey and Identification of Melanoleuca angelesiana A.H. Sm. As a First Record. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 18(3):314-326. Doi:10.17475/kastorman.499076
  • Kuo, M. and Methven, S.A. (2014). Mushrooms of the Midwest, University of Illinois Press. Urbana, Chicago and Springfield.
  • Nawaz, F., Jabeen, S., Khalid, A.N. (2017). New and noteworthy Melanoleuca (Pluteaceae) from Pakistan. Phytotaxa, 311 (2): 175-184. DOI: https://doi.org/10.11646/phytotaxa.311.2.5
  • Nguyen, L.T., Schmidt, H.A., von Haeseler, A., Minh B.Q. (2015). IQ-TREE A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32: 268-274.
  • Saba, M. and Khalid, A.N. (2014). New record of Melanoleuca cinereifolia in Himalayan moist temperate forests of Pakistan. Mycotaxon, 129(2):317-327. DOI: https://doi.org/10.5248/129.317
  • Sánchez-García, M., Blanco, J.C., Matheny, P.B. (2013). Taxonomic Revision of the Genus Melanoleuca in Mexico and Description of New Species. Revista Mexicana de Biodiversidad, 111-127. https://doi.org/10.7550/rmb.31569
  • Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesqueb, C.A., Chen, W. and Fungal Barcoding Consortium. (2012). Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. PNAS, 109:6241-6246. https://doi.org/10.1073/pnas.1117018109
  • Sesli, E., Asan, A., Selçuk, F. (eds), Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G., Halikî Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu., and Yoltaş, A. (2020). Türkiye Mantarları Listesi (The Checklist of Fungi of Turkey), Ali Nihat Gökyiğit Vakfı Yayını. İstanbul.
  • Tamura, K., Stecher, G., Peterson, D., Filipski, A.M., Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology Evolution, 30(12):2725-2729.
  • Thompson, J.D., Higgins, D.G., Gibson, T.J. (1994). Clustal W improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680. doi: 10.1093/nar/22.22.4673
  • Vesterholt, J. (2008). Funga Nordica-Agaricoid, boletoid and cyphelloid genera, Nordsvamp, Copenhagen. Vizzini, A., Para, R., Fontenla, R., Ghignone, S., Ercole, E. (2011). A preliminary ITS phylogeny of Melanoleuca (Agaricales) with special reference to European taxa. Mycotaxon, 118: 361-381. DOI: 10.5248/118.361
  • Wen, J., Zimmer, E.A. (1996). Phylogeny and Biogeography of Panax L. (the Ginseng Genus, Araliaceae): Inferences from ITS Sequences of Nuclear Ribosomal DNA. Molecular Phylogenetics and Evolution, 6, 167–177.
  • Wu, F., Zhou, L., Yang, Z., Bau, T., Li, T.H., Dai, Y. (2019). Resource diversity of Chinese macrofungi: edible, medicinal and poisonous species. Fungal Diversity, 98:1–76. https://doi.org/10.1007/s13225-019-00432-7
  • Yu, X., Lv, S., Ma, D., Li, F., Lin, Y., Zhang, L. (2014). Two new species of Melanoleuca (Agaricales, Basidiomycota) from northeastern China, supported by morphological and molecular data. Mycoscience, 55: 456-461.

Molecular and Morphological Identification of Melanoleuca cinereifolia from Türkiye

Yıl 2022, Cilt: 13 Sayı: 2, 134 - 140, 31.10.2022

Ayşenur Kalmer Ayten Dizkırıcı İsmail Acar

Öz

Melanoleuca cinereifolia is identified based on macro/micro-morphological characters and DNA sequences of the nuclear ribosomal Internal Transcribed Spacer (nrITS) region and is reported here for the first time from Diyarbakır province of Türkiye. Macro- and micro-morphological description of the species is provided along with the photographs of fresh basidiomes in natural habitat and the line drawings. The species is mainly characterized by basidiome with a short stipe, grayish-brown pileus, grey lamellae and lageniform cystidia. In the phylogram, M. cinereifolia is clustered with its allies in the subgenus Melanoleuca. Morphological and molecular similarities and differences among the species and the close relatives were discussed in detail.

Anahtar Kelimeler

DNA ITS Molecular taxonomy Agaricales

Kaynakça

  • Acar, I., Dizkirici Tekpınar, A., Kalmer, A., Uzun, Y. (2017). Phylogenetic relationships and taxonomical positions of two new records Melanoleuca species from Hakkâri province, Turkey. Biodicon, 10(3): 85-93.
  • Antonín, V., Ďuriška, O., Gafforov, Y., Jančovičová, S., Para, R., Tomšovský, M. (2017). Molecular phylogenetics and taxonomy in Melanoleuca exscissa group, (Tricholomataceae, Basidiomycota) and the description of M. griseobrunnea sp. nov. Plant Syst Evol, 303:1181-1198. DOI 10.1007/s00606-017-1430-y
  • Antonín, V., Ďuriška, O., Jančovičová, S., Para, R., Kudláček, T., Tomšovský, M. (2021). Multilocus phylogeny and taxonomy of European Melanoleuca subgenus Melanoleuca. Mycologia, DOI: 10.1080/00275514.2021.1966246
  • Bahadori, M.B., Sarikurkcu, C., Yalcin, O., Cengiz, M., Gungor, H. (2019). Metal concentration, phenolics profiling, and antioxidant activity of two wild edible Melanoleuca mushrooms (M. cognata and M. stridula). Microchemical Journal, 150:104172. DOI: 10.1016/j.microc.2019.104172
  • Breitenbach, J. and Kränzlin, F. (1991). Fungi of Switzerland. Verlag Mykologia. 3rd ed. Lucerne, Switzerland.
  • Boekhout, T. (1999). Melanoleuca, in: C. Bas et al. (eds). Flora Agaricina Neerlandica 4.A.A. Balkema, Rotterdam, pp. 153-165.
  • Bon, M. (1991). Flore mycologiqued’Europe, 2 – Les Tricholomesetressemblants. Revue de Mycologie Mémóire Hors-Série, 2, 163.
  • Buczacki, S. (2012). Collins Fungi Guide: The Most Complete Field Guide to the Mushrooms and Toadstools of Britain & Ireland. Harper Collins Publishers, London, UK.
  • Doyle, J.J., Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19,11-15.
  • Ďuriška, O., Antonín, V., Para, R., Tomšovsky, M., Jančovičova, S. (2017). Taxonomy, ecology and distribution of Melanoleuca strictipes (Basidiomycota, Agaricales) in Europe. Czech Mycology, 69(1): 15-30.
  • Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W., Gascuel, O. (2010). New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Systematic Biology, 59(3):307-21. https://doi.org/10.1093/sysbio/syq010
  • Hoang, D.T., Chernomor, O., von Haeseler, A., Minh, B.Q., Vinh, L.S. (2018) UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35:518–522. https://doi.org/10.1093/molbev/msx281
  • Kalmer, A., Acar, I., Dizkirici Tekpınar, A. (2018). Phylogeny of Some Melanoleuca Species (Fungi: Basidiomycota) in Turkey and Identification of Melanoleuca angelesiana A.H. Sm. As a First Record. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 18(3):314-326. Doi:10.17475/kastorman.499076
  • Kuo, M. and Methven, S.A. (2014). Mushrooms of the Midwest, University of Illinois Press. Urbana, Chicago and Springfield.
  • Nawaz, F., Jabeen, S., Khalid, A.N. (2017). New and noteworthy Melanoleuca (Pluteaceae) from Pakistan. Phytotaxa, 311 (2): 175-184. DOI: https://doi.org/10.11646/phytotaxa.311.2.5
  • Nguyen, L.T., Schmidt, H.A., von Haeseler, A., Minh B.Q. (2015). IQ-TREE A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32: 268-274.
  • Saba, M. and Khalid, A.N. (2014). New record of Melanoleuca cinereifolia in Himalayan moist temperate forests of Pakistan. Mycotaxon, 129(2):317-327. DOI: https://doi.org/10.5248/129.317
  • Sánchez-García, M., Blanco, J.C., Matheny, P.B. (2013). Taxonomic Revision of the Genus Melanoleuca in Mexico and Description of New Species. Revista Mexicana de Biodiversidad, 111-127. https://doi.org/10.7550/rmb.31569
  • Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesqueb, C.A., Chen, W. and Fungal Barcoding Consortium. (2012). Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. PNAS, 109:6241-6246. https://doi.org/10.1073/pnas.1117018109
  • Sesli, E., Asan, A., Selçuk, F. (eds), Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G., Halikî Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu., and Yoltaş, A. (2020). Türkiye Mantarları Listesi (The Checklist of Fungi of Turkey), Ali Nihat Gökyiğit Vakfı Yayını. İstanbul.
  • Tamura, K., Stecher, G., Peterson, D., Filipski, A.M., Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology Evolution, 30(12):2725-2729.
  • Thompson, J.D., Higgins, D.G., Gibson, T.J. (1994). Clustal W improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680. doi: 10.1093/nar/22.22.4673
  • Vesterholt, J. (2008). Funga Nordica-Agaricoid, boletoid and cyphelloid genera, Nordsvamp, Copenhagen. Vizzini, A., Para, R., Fontenla, R., Ghignone, S., Ercole, E. (2011). A preliminary ITS phylogeny of Melanoleuca (Agaricales) with special reference to European taxa. Mycotaxon, 118: 361-381. DOI: 10.5248/118.361
  • Wen, J., Zimmer, E.A. (1996). Phylogeny and Biogeography of Panax L. (the Ginseng Genus, Araliaceae): Inferences from ITS Sequences of Nuclear Ribosomal DNA. Molecular Phylogenetics and Evolution, 6, 167–177.
  • Wu, F., Zhou, L., Yang, Z., Bau, T., Li, T.H., Dai, Y. (2019). Resource diversity of Chinese macrofungi: edible, medicinal and poisonous species. Fungal Diversity, 98:1–76. https://doi.org/10.1007/s13225-019-00432-7
  • Yu, X., Lv, S., Ma, D., Li, F., Lin, Y., Zhang, L. (2014). Two new species of Melanoleuca (Agaricales, Basidiomycota) from northeastern China, supported by morphological and molecular data. Mycoscience, 55: 456-461.
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Ayşenur Kalmer 0000-0001-6176-8812

Ayten Dizkırıcı 0000-0002-0578-5092

İsmail Acar 0000-0002-6049-4896

Yayımlanma Tarihi 31 Ekim 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 13 Sayı: 2

Kaynak Göster

APA Kalmer, A., Dizkırıcı, A., & Acar, İ. (2022). Molecular and Morphological Identification of Melanoleuca cinereifolia from Türkiye. Mantar Dergisi, 13(2), 134-140. https://doi.org/10.30708/mantar.1125768
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