Öz
Bisphenol-A, bugün epoksi reçine üretiminde ve diğer non-polimer plastiklerde katkı maddesi olarak kullanılan östrojenik bir kimyasaldır. BPA'nın bugünkü yaygın kullanımı nedeniyle, insanların maruz kalması kaçınılmazdır. Bu maruziyet, çeşitli vücut sistemlerinde zararlı etkilere neden olmaktadır. Bu çalışmanın amacı, gebelik ve emzirme döneminde BPA'ya maruz kalan anne sıçanların yavrularının böbreklerinin gelişimi üzerindeki etkilerini araştırmaktır, çünkü yavrular plasenta ve süt yoluyla BPA'ya maruz kalmaktadır. Bu çalışmada, 13 yetişkin Wistar albino dişi sıçan 3 gruba ayrıldı. Grup 1'de (Kontrol grubu) sıçanlara sadece 1 ml/kg/gün mısır yağı intraperitoneal olarak uygulandı. Grup 2'deki (25 mg BPA grubu) sıçanlara 25 mg/kg/gün BPA; Grup 3'teki (50 mg grup) sıçanlara 5 hafta boyunca intraperitoneal olarak 50 mg/kg/gün BPA uygulandı. Deneyin sonunda, yavru sıçanların intrakardiyak kanı ve böbrek dokuları alındı ve üre, total protein, kreatinin, TAS, TOS, MDA değerleri incelendi. Çalışmanın sonunda, BPA'nın serum üre, kreatinin ve total protein seviyelerini artırdığı, böbrek dokusunda oksidatif hasara neden olan reaktif oksijen türlerinin oluşumunu indüklediği ve yavru sıçanların böbreklerinde, Bowman'ın boşluğunda daralma gibi ciddi yapısal hasarlara neden olduğu belirlendi. böbrek korpuskülü, proksimal, distal tübüller ve toplayıcı kanallarda genişleme, tübüler epitelde hücre kaybı ve vaküolizasyon.
Anahtar Kelimeler
Destekleyen Kurum
Pamukkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi
Proje Numarası
2020SABE023
Teşekkür
Çalışmamıza sağladığı fon desteğinden dolayı Pamukkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi'ne teşekkür ederiz.
Kaynakça
- 1. Esplugas R, Llovet MI, Bellés M, et al. Renal and hepatic effects following neonatal exposure to low doses of Bisphenol-A and 137Cs. Food Chem Toxicol. 2018;114:270-7.
- 2. Çelik Y, Şahin S. Health effects of bisphenol a as an endocrine disrupting chemical. Sürekli Tıp Eğitimi Dergisi 2020;29:439-45.
- 3. Wang M, Rang O, Liu F, et al. A systematic review of metabolomics biomarkers for Bisphenol A exposure. Metabolomics. 2018;14:45.
- 4. Nakamura K, Itoh K, Sugimoto T, Fushiki S. Prenatal exposure to bisphenol A affects adult murine neocortical structure. Neurosci Lett. 2007;420:100-5.
- 5. Rezg R, El-Fazaa S, Gharbi N, Mornagui B. Bisphenol A and human chronic diseases: current evidences, possible mechanisms, and future perspectives. Environ Int. 2014;64:83-90.
- 6. Ola-Davies OE, Olukole SG. Gallic acid protects against bisphenol A-induced alterations in the cardio-renal system of Wistar rats through the antioxidant defense mechanism. Biomed Pharmacother. 2018;107:1786-94.
- 7. Poormoosavi SM, Najafzadehvarzi H, Behmanesh MA, Amirgholami R. Protective effects of Asparagus officinalis extract against Bisphenol A- induced toxicity in Wistar rats. Toxicol Rep. 2018;5:427-33.
- 8. Yaprak M, Bay F, Turgut FH. Endocrine disruptors and kidney. Turkiye Klinikleri J Endocrin-Special Topics. 2017;9:45-9.
- 9. Yuan J, Kong Y, Ommati MM, et al. Bisphenol A-induced apoptosis, oxidative stress and DNA damage in cultured rhesus monkey embryo renal epithelial Marc-145 cells. Chemosphere. 2019;234:682-9.
- 10. Ayazgök B, Küçükkilinç TT. big effects of low dose Bisphenol A. FABAD J Pharm Sci. 2017;42:139-50.
- 11. Kovacic P. How safe is bisphenol A? Fundamentals of toxicity: metabolism, electron transfer and oxidative stress. Med Hypotheses. 2010;75:1-4.
- 12. Kobroob A, Peerapanyasut W, Chattipakorn N, Wongmekiat O. Damaging effects of bisphenol A on the kidney and the protection by melatonin: emerging evidences from in vivo and in vitro studies. Oxid Med Cell Longev. 2018;2018:3082438.
- 13. Aydos Z, Boyacioğlu M. The Investigaton of the protective effect of folic acid on experimental Bisphenol A toxication in rats. Animal Health Production and Hygiene. 2019;8:642-6.
- 14. Edres HA, Taha NM, Mandour A, Lebda M. Impact of L-Carnitine on Bisphenol A-induced kidney damage in rats. Alexandria Journal of Veterinary Sciences. 2018;56:11-7.
- 15. Shin BS, Yoo SD, Cho CY, et al. Maternal-fetal disposition of bisphenol a in pregnant Sprague-Dawley rats. J Toxicol Environ Health A. 2002;65:395-406.
- 16. Kabuto H, Amakawa M, Shishibori T. Exposure to bisphenol A during embryonic/fetal life and infancy increases oxidative injury and causes underdevelopment of the brain and testis in mice. Life Sci. 2004;74:2931-40.
Öz
Aim: Bisphenol-A (BPA) is an estrogenic chemical used today in the production of epoxy resin and as an additive in other non-polymer plastics. Due to the widespread use of BPA today, human exposure is inevitable. This exposure causes harmful effects on various body systems. The aim of this study is to investigate the effects on the development of the kidneys of the offspring of mother rats exposed to BPA during pregnancy and lactation, as a result of the offspring being exposed to BPA through the placenta and milk.
Material and Methods: In this study, 13 adult Wistar albino female rats were divided into 3 groups. In Group 1 (Control group), rats were only administered 1 ml/kg/day corn oil intraperitoneally. Group 2 (25 mg BPA group) rats were administered 25 mg/kg/day BPA; Group 3 (50 mg group) rats were administered 50 mg/kg/day BPA intraperitoneally for 5 weeks. At the end of the experiment, the intracardiac blood and kidney tissues of the offspring rats were taken and examined for urea, total protein, creatinine, TAS, TOS, MDA values.
Results: At the end of the study, it was determined that BPA increased serum urea, creatinine and total protein levels, induced the formation of reactive oxygen species causing oxidative damage in kidney tissue, and caused serious structural damages
Conclusion: Only mother rats exposed to BPA. BPA transferred to pups via placenta and milk, causing structural damage: narrowing in Bowman's space of renal corpuscle, dilatation in proximal/distal tubules and collecting ducts, occasional cell loss, vacuolization in tubule epithelia.
Anahtar Kelimeler
Proje Numarası
2020SABE023
Kaynakça
- 1. Esplugas R, Llovet MI, Bellés M, et al. Renal and hepatic effects following neonatal exposure to low doses of Bisphenol-A and 137Cs. Food Chem Toxicol. 2018;114:270-7.
- 2. Çelik Y, Şahin S. Health effects of bisphenol a as an endocrine disrupting chemical. Sürekli Tıp Eğitimi Dergisi 2020;29:439-45.
- 3. Wang M, Rang O, Liu F, et al. A systematic review of metabolomics biomarkers for Bisphenol A exposure. Metabolomics. 2018;14:45.
- 4. Nakamura K, Itoh K, Sugimoto T, Fushiki S. Prenatal exposure to bisphenol A affects adult murine neocortical structure. Neurosci Lett. 2007;420:100-5.
- 5. Rezg R, El-Fazaa S, Gharbi N, Mornagui B. Bisphenol A and human chronic diseases: current evidences, possible mechanisms, and future perspectives. Environ Int. 2014;64:83-90.
- 6. Ola-Davies OE, Olukole SG. Gallic acid protects against bisphenol A-induced alterations in the cardio-renal system of Wistar rats through the antioxidant defense mechanism. Biomed Pharmacother. 2018;107:1786-94.
- 7. Poormoosavi SM, Najafzadehvarzi H, Behmanesh MA, Amirgholami R. Protective effects of Asparagus officinalis extract against Bisphenol A- induced toxicity in Wistar rats. Toxicol Rep. 2018;5:427-33.
- 8. Yaprak M, Bay F, Turgut FH. Endocrine disruptors and kidney. Turkiye Klinikleri J Endocrin-Special Topics. 2017;9:45-9.
- 9. Yuan J, Kong Y, Ommati MM, et al. Bisphenol A-induced apoptosis, oxidative stress and DNA damage in cultured rhesus monkey embryo renal epithelial Marc-145 cells. Chemosphere. 2019;234:682-9.
- 10. Ayazgök B, Küçükkilinç TT. big effects of low dose Bisphenol A. FABAD J Pharm Sci. 2017;42:139-50.
- 11. Kovacic P. How safe is bisphenol A? Fundamentals of toxicity: metabolism, electron transfer and oxidative stress. Med Hypotheses. 2010;75:1-4.
- 12. Kobroob A, Peerapanyasut W, Chattipakorn N, Wongmekiat O. Damaging effects of bisphenol A on the kidney and the protection by melatonin: emerging evidences from in vivo and in vitro studies. Oxid Med Cell Longev. 2018;2018:3082438.
- 13. Aydos Z, Boyacioğlu M. The Investigaton of the protective effect of folic acid on experimental Bisphenol A toxication in rats. Animal Health Production and Hygiene. 2019;8:642-6.
- 14. Edres HA, Taha NM, Mandour A, Lebda M. Impact of L-Carnitine on Bisphenol A-induced kidney damage in rats. Alexandria Journal of Veterinary Sciences. 2018;56:11-7.
- 15. Shin BS, Yoo SD, Cho CY, et al. Maternal-fetal disposition of bisphenol a in pregnant Sprague-Dawley rats. J Toxicol Environ Health A. 2002;65:395-406.
- 16. Kabuto H, Amakawa M, Shishibori T. Exposure to bisphenol A during embryonic/fetal life and infancy increases oxidative injury and causes underdevelopment of the brain and testis in mice. Life Sci. 2004;74:2931-40.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Histoloji ve Embriyoloji |
| Bölüm | Özgün Makaleler |
| Yazarlar | |
| Proje Numarası | 2020SABE023 |
| Erken Görünüm Tarihi | 23 Ağustos 2023 |
| Yayımlanma Tarihi | 18 Eylül 2023 |
| Kabul Tarihi | 29 Temmuz 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 5 Sayı: 3 |
Chief Editors
Assoc. Prof. Zülal Öner
Address: İzmir Bakırçay University, Department of Anatomy, İzmir, Turkey
Assoc. Prof. Deniz Şenol
Address: Düzce University, Department of Anatomy, Düzce, Turkey
E-mail: medrecsjournal@gmail.com
Publisher:
Medical Records Association (Tıbbi Kayıtlar Derneği)
Address: Düzce / Türkiye
Publication Support:
Effect Publishing & Agency
Phone: + 90 (553) 610 67 80
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