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Kronik Stres ve Böbrek: Sıçan Modeli Üzerinde Morfometrik ve Histopatolojik Çalışma

Yıl 2017, Cilt: 7 Sayı: 4, 168 - 175, 04.01.2018
https://doi.org/10.31832/smj.366673

Öz















Amaç: Günlük yaşamın bir parçası haline gelen stres, vücudun dengesini bozarak
pek çok hastalığa neden olur. Biz de bu çalışmada, kronik strese sebep olan
faktörlere maruz kalındığında böbrekte meydana gelebilecek olası yapısal
değişiklikleri araştırmayı amaçladık.




Gereç ve Yöntem: Bu çalışmada 12 adet Sprague Dawley cinsi yetişkin erkek sıçan
kullanıldı. Sıçanlar rastgele kontrol (n=6) ve stres (n=6) grubu olarak ikiye
ayrıldıktan sonra, stres grubundakilere 4 hafta boyunca “beklenmedik kronik
hafif stres” protokolü uygulandı. Deneyin sonunda anestezisi altında feda
edilen bütün sıçanların böbrekleri çıkarıldı ve derhal “suya daldırma yöntemi”
ile hacimleri ölçüldü. Böbrekler rutin histolojik işlemleri takiben,
histopatolojik değerlendirme için ışık mikroskobunda incelendi.




Bulgular: Stres grubundaki sıçanların böbrek hacimlerinin (0,90 ± 0,096 ml)
kontrol grubundakilerin böbrek hacimlerine (1,075 ± 0,106 ml) kıyasla önemli
düzeyde azalmış olduğu
(p<0,05;
independent samples t test)  tespit
edildi. Stres grubu böbreklerinin ışık mikroskobik değerlendirmesinde, nefron
tübüllerinin epitelinde nekrotik dejenerasyon ve dökülmeler saptandı. Proksimal
ve distal tübüllerin epitelinde, yer yer hücresel parçalanma ve tübül duvar
bütünlüğünün bozulması ile sonuçlanan sitoplazmik şişme ve hücresel
vakuolleşmeler belirgindi. Henle kulpunda da lümende bariz dilatasyonlar ve
epitelde nekrotik değişiklikler tespit edildi. İlaveten glomerüllerde büzüşme
ve Bowman boşluğunda genişlemeler gözlendi.




Sonuç: Elde edilen bulgulara dayanarak, kronik stresin böbrekte büyük olasılıkla
fonksiyonel yetersizliğe yol açacak glomerüler hasara ve tübül epitel
hücrelerinde zedelenmeye sebep olduğu düşünüldü.








Kaynakça

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Toplam 57 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm Makaleler
Yazarlar

Elvan Şahin Bu kişi benim

Tuba Demirci

Semin Gedikli

Yayımlanma Tarihi 4 Ocak 2018
Gönderilme Tarihi 15 Aralık 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 7 Sayı: 4

Kaynak Göster

AMA Şahin E, Demirci T, Gedikli S. Kronik Stres ve Böbrek: Sıçan Modeli Üzerinde Morfometrik ve Histopatolojik Çalışma. Sakarya Tıp Dergisi. Ocak 2018;7(4):168-175. doi:10.31832/smj.366673

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