Effect of Wheat Germ Oil on Wound Healing: An In Vitro Study in Fibroblast Cells

Muhammed Gömeç; Gülnihal İpek; Ayşegül Öztürk; Deniz Şahin İnan

doi:10.51972/tfsd.1128533

Araştırma Makalesi

Effect of Wheat Germ Oil on Wound Healing: An In Vitro Study in Fibroblast Cells

Yıl 2022, Cilt: 3 Sayı: 3, 230 - 235, 30.09.2022

Muhammed Gömeç Gülnihal İpek Ayşegül Öztürk Deniz Şahin İnan

https://doi.org/10.51972/tfsd.1128533

Öz

Purpose: Wound healing is a set of mechanisms that are activated to restore structurally damaged tissue. There are many studies aimed at accelerating wound healing. In this context, products obtained from plants come to the fore. In this article, the effects of wheat germ oil (WGO), which is known to have antioxidant and anti-inflammatory properties, on wound healing were investigated by in vitro method.
Material and Methods: Wheat germ oil and α-tocopherol were applied to L929, a healthy fibroblast cell line, at different doses for 24, 48 and 72 hours. Cell viability was measured by XTT colorimetric method. In vitro wound healing model was applied at the dose where the oil was effective. Obtained results were analyzed statistically.
Results: As a result of the application of α-tocopherol to L929 cells at different doses, it was observed that there was no significant contribution to cell proliferation compared to the control group. However, WGO was observed to significantly increase proliferation at the 100 ng/ml concentration. In the wound healing model, cells treated with WGO at 48 hours were observed to proliferate faster and invade the wound site more rapidly. (p<0.05)
Conclusion: The data obtained in our study showed that WGO contributed positively to wound healing. It was determined that this effect was not related to the α-tocopherol in the content of WGO. Although the effect of WGO on wound healing was seen to be positive in our study, our study should be supported by in vivo and clinical studies.

Anahtar Kelimeler

Cell viability, Wheat germ oil, Wound healing, α-tokoferole.

Destekleyen Kurum

TÜBİTAK

Proje Numarası

1919B012102114

Teşekkür

We thank CUTFAM, the center where the experiment was conducted.

Kaynakça

Abdel-Gawad, S. K. (2015). Therapeutic and protective effect of wheat germ oil on l-arginine induced acute pancreatitis in adult Albino rats. Journal of Cell Science & Therapy(S8), 1.
Anwar, M., & Mohamed, N. (2015). Amelioration of liver and kidney functions disorders induced by sodium nitrate in rats using wheat germ oil. Journal of Radiation Research and Applied Sciences, 8(1), 77-83.
Anwar, M. M., & Mohamed, N. E. (2015). Amelioration of liver and kidney functions disorders induced by sodium nitrate in rats using wheat germ oil. Journal of Radiation Research and Applied Sciences, 8(1), 77-83. doi:https://doi.org/10.1016/j.jrras.2014.11.004
Atwell, W., & Finnie, S. (2016). Wheat flour: Elsevier.
Barrientos, S., Stojadinovic, O., Golinko, M. S., Brem, H., & Tomic-Canic, M. (2008). PERSPECTIVE ARTICLE: Growth factors and cytokines in wound healing. Wound repair and regeneration, 16(5), 585-601. doi:https://doi.org/10.1111/j.1524-475X.2008.00410.x
Borges, G. Á., Elias, S. T., da Silva, S. M. M., Magalhães, P. O., Macedo, S. B., Ribeiro, A. P. D., & Guerra, E. N. S. (2017). In vitro evaluation of wound healing and antimicrobial potential of ozone therapy. Journal of Cranio-Maxillofacial Surgery, 45(3), 364-370. doi:https://doi.org/10.1016/j.jcms.2017.01.005
Eisenmenger, M., & Dunford, N. T. (2008). Bioactive Components of Commercial and Supercritical Carbon Dioxide Processed Wheat Germ Oil. Journal of the American Oil Chemists' Society, 85(1), 55-61. doi:10.1007/s11746-007-1163-0
Falanga, V. (2005). Wound healing and its impairment in the diabetic foot. The Lancet, 366(9498), 1736-1743.
Frykberg, R., & Banks, J. (2015). Challenges in the Treatment of Chronic Wounds. Advances in Wound Care. 2015: 4 (9): 560–582.
Gurtner, G. C., Werner, S., Barrandon, Y., & Longaker, M. T. (2008). Wound repair and regeneration. Nature, 453(7193), 314-321. doi:10.1038/nature07039
Hamdi, H. (2019). The preventive role of wheat germ oil against sertraline-induced testicular damage in male albino rats. Andrologia, 51(10), e13369. doi:https://doi.org/10.1111/and.13369
Jacob, R. A. (1995). The integrated antioxidant system. Nutrition research, 15(5), 755-766.
Leenhardt, F., Fardet, A., Lyan, B., Gueux, E., Rock, E., Mazur, A., . . . Rémésy, C. (2008). Wheat germ supplementation of a low vitamin E diet in rats affords effective antioxidant protection in tissues. Journal of the American College of Nutrition, 27(2), 222-228.
Liang, C.-C., Park, A. Y., & Guan, J.-L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333. doi:10.1038/nprot.2007.30
Liu, R. H. (2007). Whole grain phytochemicals and health. Journal of Cereal Science, 46(3), 207-219. doi:https://doi.org/10.1016/j.jcs.2007.06.010
Mahmoud, A. A., Mohdaly, A. A. A., & Elneairy, N. A. A. (2015). Wheat Germ: An Overview on Nutritional Value, Antioxidant Potential and Antibacterial Characteristics. Food and Nutrition Sciences, Vol.06No.02, 13. doi:10.4236/fns.2015.62027
Nicolaus, C., Junghanns, S., Hartmann, A., Murillo, R., Ganzera, M., & Merfort, I. (2017). In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts. Journal of Ethnopharmacology, 196, 94-103. doi:https://doi.org/10.1016/j.jep.2016.12.006
Özcan, M., Rosa, A., Dessi, M. A., Marongıu, B., Pıras, A., & Juhaimi, F. A. (2013). Quality of wheat germ oil obtained by cold pressing and supercritical carbon dioxide extraction. Czech Journal of Food Sciences, 31(3), 236-240.
Sendi, P., & McNally, M. A. (2016). Wound Irrigation in Initial Management of Open Fractures. New England journal of medicine NEJM, 374(18), 1788.
Türkoğlu, G. C., Sarıışık, M., Karavana, S. Y., & Aydın Köse, F. (2021). Production of wheat germ oil containing multilayer hydrogel dressing. Carbohydrate Polymers, 269, 118287. doi:https://doi.org/10.1016/j.carbpol.2021.118287
Van Zanten, M. C., Mistry, R. M., Suami, H., Campbell-Lloyd, A., Finkemeyer, J. P., Piller, N. B., & Caplash, Y. (2017). The lymphatic response to injury with soft-tissue reconstruction in high-energy open tibial fractures of the lower extremity. Plastic and reconstructive surgery, 139(2), 483-491.
Walter, M. N. M., Wright, K. T., Fuller, H. R., MacNeil, S., & Johnson, W. E. B. (2010). Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays. Experimental Cell Research, 316(7), 1271-1281. doi:https://doi.org/10.1016/j.yexcr.2010.02.026
Wang, T., & Johnson, L. A. (2001). Refining high-free fatty acid wheat germ oil. Journal of the American Oil Chemists' Society, 78(1), 71-76.
Yuldasheva, N. K., Ul’chenko, N. T., & Glushenkova, A. I. (2010). Wheat germ oil. Chemistry of Natural Compounds, 46(1), 97-98. doi:10.1007/s10600-010-9536-2
Zakaria, R., J Musa, R., Faraj, J., H Mahmoud Abudayeh, Z., & A Mohammed, H. (2021). Evaluation of the Wheat Germ Oil Topical Formulations for Wound Healing Activity in Rats. Pakistan journal of biological sciences : PJBS, 24(6), 706-715. doi:10.3923/pjbs.2021.706.715

Yıl 2022, Cilt: 3 Sayı: 3, 230 - 235, 30.09.2022

Muhammed Gömeç Gülnihal İpek Ayşegül Öztürk Deniz Şahin İnan

https://doi.org/10.51972/tfsd.1128533

Öz

Proje Numarası

1919B012102114

Kaynakça

Abdel-Gawad, S. K. (2015). Therapeutic and protective effect of wheat germ oil on l-arginine induced acute pancreatitis in adult Albino rats. Journal of Cell Science & Therapy(S8), 1.
Anwar, M., & Mohamed, N. (2015). Amelioration of liver and kidney functions disorders induced by sodium nitrate in rats using wheat germ oil. Journal of Radiation Research and Applied Sciences, 8(1), 77-83.
Anwar, M. M., & Mohamed, N. E. (2015). Amelioration of liver and kidney functions disorders induced by sodium nitrate in rats using wheat germ oil. Journal of Radiation Research and Applied Sciences, 8(1), 77-83. doi:https://doi.org/10.1016/j.jrras.2014.11.004
Atwell, W., & Finnie, S. (2016). Wheat flour: Elsevier.
Barrientos, S., Stojadinovic, O., Golinko, M. S., Brem, H., & Tomic-Canic, M. (2008). PERSPECTIVE ARTICLE: Growth factors and cytokines in wound healing. Wound repair and regeneration, 16(5), 585-601. doi:https://doi.org/10.1111/j.1524-475X.2008.00410.x
Borges, G. Á., Elias, S. T., da Silva, S. M. M., Magalhães, P. O., Macedo, S. B., Ribeiro, A. P. D., & Guerra, E. N. S. (2017). In vitro evaluation of wound healing and antimicrobial potential of ozone therapy. Journal of Cranio-Maxillofacial Surgery, 45(3), 364-370. doi:https://doi.org/10.1016/j.jcms.2017.01.005
Eisenmenger, M., & Dunford, N. T. (2008). Bioactive Components of Commercial and Supercritical Carbon Dioxide Processed Wheat Germ Oil. Journal of the American Oil Chemists' Society, 85(1), 55-61. doi:10.1007/s11746-007-1163-0
Falanga, V. (2005). Wound healing and its impairment in the diabetic foot. The Lancet, 366(9498), 1736-1743.
Frykberg, R., & Banks, J. (2015). Challenges in the Treatment of Chronic Wounds. Advances in Wound Care. 2015: 4 (9): 560–582.
Gurtner, G. C., Werner, S., Barrandon, Y., & Longaker, M. T. (2008). Wound repair and regeneration. Nature, 453(7193), 314-321. doi:10.1038/nature07039
Hamdi, H. (2019). The preventive role of wheat germ oil against sertraline-induced testicular damage in male albino rats. Andrologia, 51(10), e13369. doi:https://doi.org/10.1111/and.13369
Jacob, R. A. (1995). The integrated antioxidant system. Nutrition research, 15(5), 755-766.
Leenhardt, F., Fardet, A., Lyan, B., Gueux, E., Rock, E., Mazur, A., . . . Rémésy, C. (2008). Wheat germ supplementation of a low vitamin E diet in rats affords effective antioxidant protection in tissues. Journal of the American College of Nutrition, 27(2), 222-228.
Liang, C.-C., Park, A. Y., & Guan, J.-L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333. doi:10.1038/nprot.2007.30
Liu, R. H. (2007). Whole grain phytochemicals and health. Journal of Cereal Science, 46(3), 207-219. doi:https://doi.org/10.1016/j.jcs.2007.06.010
Mahmoud, A. A., Mohdaly, A. A. A., & Elneairy, N. A. A. (2015). Wheat Germ: An Overview on Nutritional Value, Antioxidant Potential and Antibacterial Characteristics. Food and Nutrition Sciences, Vol.06No.02, 13. doi:10.4236/fns.2015.62027
Nicolaus, C., Junghanns, S., Hartmann, A., Murillo, R., Ganzera, M., & Merfort, I. (2017). In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts. Journal of Ethnopharmacology, 196, 94-103. doi:https://doi.org/10.1016/j.jep.2016.12.006
Özcan, M., Rosa, A., Dessi, M. A., Marongıu, B., Pıras, A., & Juhaimi, F. A. (2013). Quality of wheat germ oil obtained by cold pressing and supercritical carbon dioxide extraction. Czech Journal of Food Sciences, 31(3), 236-240.
Sendi, P., & McNally, M. A. (2016). Wound Irrigation in Initial Management of Open Fractures. New England journal of medicine NEJM, 374(18), 1788.
Türkoğlu, G. C., Sarıışık, M., Karavana, S. Y., & Aydın Köse, F. (2021). Production of wheat germ oil containing multilayer hydrogel dressing. Carbohydrate Polymers, 269, 118287. doi:https://doi.org/10.1016/j.carbpol.2021.118287
Van Zanten, M. C., Mistry, R. M., Suami, H., Campbell-Lloyd, A., Finkemeyer, J. P., Piller, N. B., & Caplash, Y. (2017). The lymphatic response to injury with soft-tissue reconstruction in high-energy open tibial fractures of the lower extremity. Plastic and reconstructive surgery, 139(2), 483-491.
Walter, M. N. M., Wright, K. T., Fuller, H. R., MacNeil, S., & Johnson, W. E. B. (2010). Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays. Experimental Cell Research, 316(7), 1271-1281. doi:https://doi.org/10.1016/j.yexcr.2010.02.026
Wang, T., & Johnson, L. A. (2001). Refining high-free fatty acid wheat germ oil. Journal of the American Oil Chemists' Society, 78(1), 71-76.
Yuldasheva, N. K., Ul’chenko, N. T., & Glushenkova, A. I. (2010). Wheat germ oil. Chemistry of Natural Compounds, 46(1), 97-98. doi:10.1007/s10600-010-9536-2
Zakaria, R., J Musa, R., Faraj, J., H Mahmoud Abudayeh, Z., & A Mohammed, H. (2021). Evaluation of the Wheat Germ Oil Topical Formulations for Wound Healing Activity in Rats. Pakistan journal of biological sciences : PJBS, 24(6), 706-715. doi:10.3923/pjbs.2021.706.715

Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Klinik Tıp Bilimleri
Bölüm	Makaleler
Yazarlar	Muhammed Gömeç 0000-0002-9127-3201 Gülnihal İpek 0000-0002-3271-0125 Ayşegül Öztürk 0000-0001-8130-7968 Deniz Şahin İnan 0000-0002-0292-4448
Proje Numarası	1919B012102114
Yayımlanma Tarihi	30 Eylül 2022
Gönderilme Tarihi	9 Haziran 2022
Kabul Tarihi	17 Eylül 2022
Yayımlandığı Sayı	Yıl 2022 Cilt: 3 Sayı: 3

Kaynak Göster

APA	Gömeç, M., İpek, G., Öztürk, A., Şahin İnan, D. (2022). Effect of Wheat Germ Oil on Wound Healing: An In Vitro Study in Fibroblast Cells. Turkish Journal of Science and Health, 3(3), 230-235. https://doi.org/10.51972/tfsd.1128533