Solanum nigrum L. (Köpek Üzümü)’un Kuraklık ve Tuz Stresine Fizyolojik Tepkilerinin Karşılaştırılması
Öz
Kuraklık ve tuzluluk, küresel iklim değişikliğinin bir sonucu olarak ortaya çıkan başlıca çevre sorunları arasında yer almaktadır. Bu çalışmada, istilacı bir yabancı ot olan köpek üzümünün (Solanum nigrum L.) değişen, gelişen kuraklık ve tuzluluk koşullarına tepkisinin antioksidan kapasite temelinde tepkisinin belirlenmesi amaçlanmıştır. Bu amaçla, Solanum nigrum tohumları petrilerde çimlendirilip perlit içeren viyollere aktarılmıştır. Fideler 42 gün boyunca Hoagland Besin Solüsyonu (%100) ile in vitro şartların sağlandığı bitki büyütme kabininde yetiştirilmiştir. Daha sonra fideler kontrol (C), kuraklık stresi (D) ve tuz stresi (100 mM NaCl) olarak üç gruba ayrılmıştır. Bir haftalık uygulamanın ardından 7. günde fidelerden örnekleme yapılmıştır. Örneklerde bağıl su içeriği (BSİ), kuru ağırlık (KA), spesifik yaprak alanı (SYA) ve hidrojen peroksit (H2O2) miktarı ölçülmüştür. Kuraklık ve tuzluluk stresi uygulamaları ile BSİ özellikle kuraklık stresiyle kontrole kıyasla %27 azalmıştır. Kuru ağırlık ise kuraklık stresiyle %54, tuz stresiyle %41 azalmıştır. Ayrıca H2O2miktarı da kuraklıkla %72, tuzlulukla %48 azalmıştır. Elde edilen bulgular, köpek üzümünün tuzluluğa kıyasla kuraklıktan daha çok etkilendiğini işaret etmektedir.
Anahtar Kelimeler
Köpek üzümü (Solanum nigrum L.) Yabancı ot Kuraklık stresi Tuz stresi
Kaynakça
- Abobatta WF. (2020). Plant responses and tolerance to combined salt and drought stress. Salt and Drought Stress Tolerance in Plants: Signaling Networks and Adaptive Mechanisms, 17-52.
- Al Khateeb W., Basahi RA., Al-Qwasemeh H. (2019). Effect of salt stress on in vitro grown Solanum nigrum L. Bulgarian Journal of Agricultural Science, 25(1).
- Alam A., Sahar A., Sameen A., Faisal MN. (2022). The effects of bioactive components in Solanum nigrum against oxidative stress in liver damage. Food Science and Technology, 42.
- Al-Qwasemeh HA. (2013). Effect of various abiotic stresses on in vitro grown Solanum nigrum L (Doctoral dissertation, Yarmouk University).
- Arnon DI. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant physiology, 24(1), 1.
- Barthakur S., Babu V., Bansal KC. (2001). Overexpression of osmotin induces proline accumulation and confers drought- and low-temperature stress tolerance in tobacco by gene transfer. Plant Cell Physiol. 45:346–350.
- Bradford M. (1976). A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
- Cheeseman JM. (2006). Hydrogen peroxide concentrations in leaves under natural conditions, J of Experimental Botany 57: 2435–44.
- Corti F., Festa M., Szabo I. (2023). Mitochondria–Chloroplast Cross Talk: A possible role for calcium and reactive oxygen species? Bioelectricity, 5(1), 39-46.
- Dong H., Ma Y., Wu H., Jiang W., Ma X. (2020). Germination of Solanum nigrum L. (black nightshade) in response to different abiotic factors. Planta Daninha, 38.
- Gagare SB., Chavan SL. (2021). Effect of Withania somnifera and Solanum nigrum on drought induced stress in wheat. International Journal of Research in Biosciences, Agriculture and Technology, 9(1):91-100.
- Heuer B. (2010). Role of Proline in Plant Response to Drought and Salinity, Handbook of Plant and Crop Stress, pp -213-238.
- Hoagland DR., Arnon DI. (1950). The water-culture method for growing plants without soil. In Circular. California Agricultural Experiment Station. 347: 32.
- Kanner J., Kinsella, JE. (1983). Lipid deterioration initiated by phagocytic cells in muscle foods: βcarotene destruction by a myeloperoxidasehydrogen peroxide halide system. Journal of Agricultural and Food Chemistry 31, 370-376.
- Khan MN., Fu C., Li J., Tao Y., Li Y., Hu J., Li Z. (2023). Seed nanopriming: How do nanomaterials improve seed tolerance to salinity and drought? Chemosphere, 310, 136911.
- Kumral N., Çobanoğlu S. (2015). Köpek üzümü bitkilerinin (Solanaceae) zararlı veya avcı akarlar için barınak bitki olma potansiyelleri. Turkish Journal of Entomology, 39(1), 91-108.
- Liao R., Zhang L. (2021). Physiological response of Solanum nigrum to salt stress. In E3S Web of Conferences (Vol. 233, p. 01140). EDP Sciences.
- Machado J., Vasconcelos MW., Soares C., Fidalgo F., Heuvelink E., Carvalho SM. (2023). Young tomato plants respond differently under single or combined mild nitrogen and water deficit: an insight into morphophysiological responses and primary metabolism. Plants, 12(5), 1181.
- Nakano Y., Asada K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and cell physiology, 22(5): 867-880.
- Sahu PK., Jayalakshmi K., Tilgam J., Gupta A., Nagaraju Y., Kumar A., Rajawat, MVS. (2022). ROS generated from biotic stress: Effects on plants and alleviation by endophytic microbes. Frontiers in Plant Science, 13, 1042936.
- Sekmen Cetinel, AH., Gokce A., Erdik E., Cetinel B., Cetinkaya N. (2021). The effect of trichoderma citrinoviride treatment under salinity combined to rhizoctonia solani infection in strawberry (Fragaria x ananassa Duch.). Agronomy. 11(8), 1589.
- Smart RE., Bingham GE. (1974). Rapid estimates of relative water content. Plant Physiology. 53(2), 258-260. Spormann S., Nadais P., Sousa F., Pinto M., Martins M., Sousa B., Soares C. (2023). Accumulation of proline in plants under contaminated soils—Are We on the Same Page? Antioxidants, 12(3), 666.
- Torun H. (2022). Adana ve Mersin ili örtüaltı domates ve biber üretim alanlarında sorun olan yabancı ot türleri, yoğunlukları ve rastlama sıklıklarının belirlenmesi. Ankara/Turkey, 329.
- Uygur FN., Koch W., Walter H. (1986). Çukurova bölgesi buğday-pamuk ekim sistemindeki önemli yabancı otların tanımı. PLTS 4(1). Josef Margraf, Aichtal.
- Wilson PJ, Thompson KEN, Hodgson JG. (1999). Specific leaf area and leaf dry matter content as alternative predictors of plant strategies. The New Phytologist, 143(1): 155-162.
- Zulfiqar F., Ashraf M. (2023). Proline alleviates abiotic stress induced oxidative stress in plants. Journal of Plant Growth Regulation, 42(8), 4629-4651.
Comparison of Physiological Responses of Solanum nigrum L. (Blackberry Nightshade) to Drought and Salt Stress
Öz
Drought and salinity are among the main environmental problems because of global climate change. Our aim of this study to determine the physiological and biochemical responses of black nightshade (Solanum nigrum L.) under drought and salt stress conditions based on antioxidant capacity. For this purpose, the seeds of Solanum nigrum were germinated in petri dishes and then transferred to viols containing perlite. The seedlings were grown in a plant growth cabinet with Hoagland Nutrient Solution (100%) for 42 days in vitro conditions. The seedlings were then divided into three groups: Control group (C), drought stress group (D) and salt stress group (100 mM NaCl). Relative water content (RWC), dry weight (DW), specific leaf area (SLA) and hydrogen peroxide (H2O2) amount were measured from the seedlings on the 7th day after one week of application. With drought and salinity stress applications, RWC decreased by 27% with drought stress compared to control. The decrease in dry weight was determined as 54% with drought stress and 41% with salt stress. In addition, the amount of H2O2decreased by 72% at drought and 48% at salinity condition. Results of the study indicated that blackberry nightshade is more affected by drought compared to salinity.
Anahtar Kelimeler
Black nightshade (Solanum nigrum L.) Weed Drought stress Salt stress
Teşekkür
Araştırma için tohumları temin eden Zir. Müh. Dr. Figen EFİL'e teşekkür ederiz.
Kaynakça
- Abobatta WF. (2020). Plant responses and tolerance to combined salt and drought stress. Salt and Drought Stress Tolerance in Plants: Signaling Networks and Adaptive Mechanisms, 17-52.
- Al Khateeb W., Basahi RA., Al-Qwasemeh H. (2019). Effect of salt stress on in vitro grown Solanum nigrum L. Bulgarian Journal of Agricultural Science, 25(1).
- Alam A., Sahar A., Sameen A., Faisal MN. (2022). The effects of bioactive components in Solanum nigrum against oxidative stress in liver damage. Food Science and Technology, 42.
- Al-Qwasemeh HA. (2013). Effect of various abiotic stresses on in vitro grown Solanum nigrum L (Doctoral dissertation, Yarmouk University).
- Arnon DI. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant physiology, 24(1), 1.
- Barthakur S., Babu V., Bansal KC. (2001). Overexpression of osmotin induces proline accumulation and confers drought- and low-temperature stress tolerance in tobacco by gene transfer. Plant Cell Physiol. 45:346–350.
- Bradford M. (1976). A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
- Cheeseman JM. (2006). Hydrogen peroxide concentrations in leaves under natural conditions, J of Experimental Botany 57: 2435–44.
- Corti F., Festa M., Szabo I. (2023). Mitochondria–Chloroplast Cross Talk: A possible role for calcium and reactive oxygen species? Bioelectricity, 5(1), 39-46.
- Dong H., Ma Y., Wu H., Jiang W., Ma X. (2020). Germination of Solanum nigrum L. (black nightshade) in response to different abiotic factors. Planta Daninha, 38.
- Gagare SB., Chavan SL. (2021). Effect of Withania somnifera and Solanum nigrum on drought induced stress in wheat. International Journal of Research in Biosciences, Agriculture and Technology, 9(1):91-100.
- Heuer B. (2010). Role of Proline in Plant Response to Drought and Salinity, Handbook of Plant and Crop Stress, pp -213-238.
- Hoagland DR., Arnon DI. (1950). The water-culture method for growing plants without soil. In Circular. California Agricultural Experiment Station. 347: 32.
- Kanner J., Kinsella, JE. (1983). Lipid deterioration initiated by phagocytic cells in muscle foods: βcarotene destruction by a myeloperoxidasehydrogen peroxide halide system. Journal of Agricultural and Food Chemistry 31, 370-376.
- Khan MN., Fu C., Li J., Tao Y., Li Y., Hu J., Li Z. (2023). Seed nanopriming: How do nanomaterials improve seed tolerance to salinity and drought? Chemosphere, 310, 136911.
- Kumral N., Çobanoğlu S. (2015). Köpek üzümü bitkilerinin (Solanaceae) zararlı veya avcı akarlar için barınak bitki olma potansiyelleri. Turkish Journal of Entomology, 39(1), 91-108.
- Liao R., Zhang L. (2021). Physiological response of Solanum nigrum to salt stress. In E3S Web of Conferences (Vol. 233, p. 01140). EDP Sciences.
- Machado J., Vasconcelos MW., Soares C., Fidalgo F., Heuvelink E., Carvalho SM. (2023). Young tomato plants respond differently under single or combined mild nitrogen and water deficit: an insight into morphophysiological responses and primary metabolism. Plants, 12(5), 1181.
- Nakano Y., Asada K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and cell physiology, 22(5): 867-880.
- Sahu PK., Jayalakshmi K., Tilgam J., Gupta A., Nagaraju Y., Kumar A., Rajawat, MVS. (2022). ROS generated from biotic stress: Effects on plants and alleviation by endophytic microbes. Frontiers in Plant Science, 13, 1042936.
- Sekmen Cetinel, AH., Gokce A., Erdik E., Cetinel B., Cetinkaya N. (2021). The effect of trichoderma citrinoviride treatment under salinity combined to rhizoctonia solani infection in strawberry (Fragaria x ananassa Duch.). Agronomy. 11(8), 1589.
- Smart RE., Bingham GE. (1974). Rapid estimates of relative water content. Plant Physiology. 53(2), 258-260. Spormann S., Nadais P., Sousa F., Pinto M., Martins M., Sousa B., Soares C. (2023). Accumulation of proline in plants under contaminated soils—Are We on the Same Page? Antioxidants, 12(3), 666.
- Torun H. (2022). Adana ve Mersin ili örtüaltı domates ve biber üretim alanlarında sorun olan yabancı ot türleri, yoğunlukları ve rastlama sıklıklarının belirlenmesi. Ankara/Turkey, 329.
- Uygur FN., Koch W., Walter H. (1986). Çukurova bölgesi buğday-pamuk ekim sistemindeki önemli yabancı otların tanımı. PLTS 4(1). Josef Margraf, Aichtal.
- Wilson PJ, Thompson KEN, Hodgson JG. (1999). Specific leaf area and leaf dry matter content as alternative predictors of plant strategies. The New Phytologist, 143(1): 155-162.
- Zulfiqar F., Ashraf M. (2023). Proline alleviates abiotic stress induced oxidative stress in plants. Journal of Plant Growth Regulation, 42(8), 4629-4651.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Yapısal Biyoloji |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Erken Görünüm Tarihi | 30 Aralık 2023 |
| Yayımlanma Tarihi | 31 Aralık 2023 |
| Gönderilme Tarihi | 27 Kasım 2023 |
| Kabul Tarihi | 26 Aralık 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 26 Sayı: 3 |
