Determination of the Effects of Helichrysum Plicatum DC. Subsp. Plicatum Ethanol Extract on Thallium Sulfate Induced Testicular Toxicity in Rat
Öz
In this study, we aimed to determine the effects of the ethanol extract of Helichrysum plicatum DC plant on Thallium Sulfate (TS)-induced testicular toxicity in rats. For this purpose, a total of 24 Sprague Dawley rats, 6 each in four groups, were used in the study. Rats were administered a single dose of TS via IP and HPE via oral gavage for 8 days. After the administrations, the rats were sacrificed and blood and testicular tissues were collected. Testicular tissues were preserved for use in biochemical, histopathological, immunohistochemistry and in situ hybridization analyzes. The cauda epididymis was trimmed by separating from the testis and the resulting liquid was used for semen analysis. The sperm motility decreased and the rate of dead and abnormal spermatozoa increased in parallel to the increase (P<0.001) in oxidative damage in the TS group. Histopathological examination revealed degenerative and necrotic changes in the TS group. Cleaved caspase 3 gene expression decreased in the control group and TS group (P<0.05). The mean testicular biopsy score (MTBS) was the lowest in the TS group (P<0.05). There was a statistical difference between the groups in terms of immunohistochemical (IHC) and in situ hybridization (IST) parameters. In conclusion, it was observed that HPE could protect against TS-induced damage in testicular tissues of rats.
Anahtar Kelimeler
Helichrysum plicatum oxidative stress rat sperm thallium sulphate
Kaynakça
- Akarsu SA, Türk G, Arkalı G, Çeribaşı AO, Yüce A. (2022). Changes in heavy metal levels, reproductive characteristics, oxidative stress markers and testicular apoptosis in rams raised around thermal power plant. Theriogenology, 179, 211-222.
- Akarsu SA, Gür C, İleritürk M, Akaras N, Küçükler S, Kandemir FM. (2023). Effect of syringic acid on oxidative stress, autophagy, apoptosis, inflammation pathways against testicular damage induced by lead acetate. Journal of Trace Elements in Medicine and Biology, 80, 127315.
- Aksu EH, Kandemir FM, Küçükler S. (2021). The effects of hesperidin on colistin‐induced reproductive damage, autophagy, and apoptosis by reducing oxidative stress. Andrologia, 53(2), e13900.
- Álvarez-Barrera L, Rodríguez-Mercado JJ, Mateos-Nava RA, Vázquez-Martínez Y, Altamirano-Lozano MA. (2019). Effect on the offspring of pregnant females CD-1 mice treated with a single thallium (I) application. Reproductive Toxicology, 90, 1-7.
- Apaydin Yildirim B, Kordali S, Terim Kapakin KA, Yildirim F, Aktas Senocak E, Altun S. (2017). Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats. Journal of Zhejiang University-Scıence B, 18(6), 501-11.
- Aslan M, Orhan DD, Orhan N, Sezik E, Yesilada E. (2007). In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats. Journal of Ethnopharmacology, 109(1), 54-9.
- Bayer R, Breitwieaser I, Ward J. (2007). Tribe Gnaphalieae (Cass.) Lecoq and Juillet (1831) In: Kadereit JW, Jeffrey C, editors. The Families and Genera of Vascular Plants, Asterales. In: Springer, Berlin.
- Belhan S, Özkaraca M, Kandemir FM, Gülyüz F, Yildirim S, Ömür AD, Yener Z. (2017). Effectiveness of hesperidin on methotrexate-induced testicular toxicity in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(5).
- Bhardwaj JK, Panchal H, Saraf P. (2021). Cadmium as a testicular toxicant: A Review. Journal of Applied Toxicology, 41(1), 105-17.
- Bigović DJ, Stević TR, Janković TR, Noveski NB, Radanović DS, Pljevljakušić DS, Djurić ZR. (2017). Antimicrobial activity of Helichrysum plicatum DC. Hemıjska Industrıja (Chemical Industry), 71(4), 337-42.
- Bramanti E, Onor M, Colombaioni L. (2018). Neurotoxicity induced by low thallium doses in living hippocampal neurons: evidence of early onset mitochondrial dysfunction and correlation with ethanol production. ACS Chemical Neuroscience, 10(1), 451-9.
- Chang Y, Tsai JF, Chen PJ, Huang YT, Liu BH. (2023). Thallium exposure interfered with heart development in embryonic zebrafish (Danio rerio): From phenotype to genotype. Science of The Total Environment, 878, 162901.
- Genchi G, Carocci A, Lauria G, Sinicropi MS, Catalano A. (2021). Thallium use, toxicity, and detoxification therapy: An overview. Applied Sciences, 11(18), 8322.
- Gibson JE, Becker BA. (1970). Placental transfer, embryotoxicity, and teratogenicity of thallium sulfate in normal and potassium-deficient rats. Toxicology and Applied Pharmacology, 16(1), 120-32.
- Gur C, Akarsu SA, Akaras N, Tuncer SC, Kandemir FM. (2023). Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite-induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats. Environmental Toxicology. 38(6),1265-76
- Gur C, Kandemir O, Kandemir FM. (2022). Investigation of the effects of hesperidin administration on abamectin‐induced testicular toxicity in rats through oxidative stress, endoplasmic reticulum stress, inflammation, apoptosis, autophagy, and JAK2/STAT3 pathways. Environmental Toxicology, 37(3), 401-12.
- Ileriturk M, Benzer F, Aksu EH, Yildirim S, Kandemir FM, Dogan T, Genc A. (2021). Chrysin protects against testicular toxicity caused by lead acetate in rats with its antioxidant, anti‐inflammatory, and antiapoptotic properties. Journal of Food Biochemistry 45(2), e13593.
- Ileriturk M, Kandemir O, Kandemir FM. (2022). Evaluation of protective effects of quercetin against cypermethrin‐induced lung toxicity in rats via oxidative stress, inflammation, apoptosis, autophagy, and endoplasmic reticulum stress pathway. Environmental Toxicology, 37(11), 2639-50.
- Khushboo M, Murthy MK, Devi MS, Sanjeev S, Ibrahim KS, Kumar NS, Gurusubramanian G. (2018). Testicular toxicity and sperm quality following copper exposure in Wistar albino rats: ameliorative potentials of L-carnitine. Environmental Science and Pollution Research, 25(2), 1837-62.
- Kulevanova S, Stefova M, Stafilov T. (2000). HPLC identification and determination of flavone aglycones in Helichrysum plicatum DC.(Asteraceae). Die Pharmazie, 55, 391–92.
- Kumar TR, Doreswamy K, Shrilatha B. (2002). Oxidative stress associated DNA damage in testis of mice: induction of abnormal sperms and effects on fertility. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 513(1-2), 103-11.
- Li D, Yao H, Du L, Zeng X, Xiao Q. (2022). Thallium (I and III) exposure leads to liver damage and disorders of fatty acid metabolism in mice. Chemosphere, 307, 135618.
- Metzstein MM, Stanfield GM, Horvitz HR. (1998). Genetics of programmed cell death in C. elegans: past, present and future. Trends in Genetics, 14(10), 410-6.
- Miloglu FD, Akpınar A, Güven L, Demirkaya AK, Gundogdu G, Nalcı KA, Hacımuftuoglu A. (2021). Evaluation the Effects of Helichrysum plicatum Subsp. pseudoplicatum on an In-Vitro Wound Model Using Human Dermal Fibroblast Cells. The International Journal of Lower Extremity Wounds, 15347346211016693.
- Sánchez-Chapul L, Santamaría A, Aschner M, Ke T, Tinkov AA, Túnez I, Rangel-López E. (2023). Thallium-induced DNA damage, genetic, and epigenetic alterations. Frontiers in Genetics, 14.
- Sevim Ç, Çomaklı S, Taghizadehghalehjoughi A, Özkaraca M, Mesnage R, Kovatsi L, Tsatsakis A. (2019). An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas. Toxicology Reports, 6, 42-50. Sindhi PI, Raval SH, Singh RD, Parmar RS, Patel JG, Patel BJ, Joshi DV. (2022). Thallium Induced Sub-chronic Toxicity Profile in Wistar Rats. Indian Journal of Veterinary Sciences and Biotechnology, 18(5), 93-9.
- Singh J, Kalamdhad AS. (2011). Effects of heavy metals on soil, plants, human health and aquatic life. Int J Res Chem Environ, 1(2), 15-21.
- Varão A, Silva J, Amaral L, Aleixo L, Onduras A, Santos C, Stiboller M. (2021). Toxic effects of thallium acetate by acute exposure to the nematode C. elegans. Journal of Trace Elements in Medicine and Biology, 68, 126848. Vermeulen K, Van Bockstaele DR, Berneman ZN. (2005). Apoptosis: mechanisms and relevance in cancer. Annals of Hematology, 84(10), 627-39.
Helichrysum Plicatum DC Subsp Plicatum Etanol Ekstraktının Sıçanlarda Talyum Sülfatın Neden Olduğu Testis Toksisitesi Üzerine Etkilerinin Belirlenmesi
Öz
Bu çalışmada, Helichrysum plicatum DC bitkisinin ratlarda Talyum Sülfat (TS) ile indüklenen testis toksisitesi üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Bu amaçla çalışmada her grupta 6 adet olmak üzere toplam 24 adet Sprague Dawley rat kullanıldı. Tedavilerin ardından ratlar sakrifiye edilerek kan ve testis dokuları alındı. Testis dokuları, biyokimyasal ve histopatolojik analizlerde kullanılmak üzere saklandı. Kauda epididim testisten ayrılarak traşlandı ve elde edilen sıvı semen analizi için kullanıldı. Çalışma bulgularımıza göre TS grubunda sperm motilitesinin azaldığı, ölü ve anormal spermatozoa oranının arttığı buna karşılık oksidatif hasarın arttığı (P<0.001) saptanmıştır. Histopatolojik incelemede TS grubunda nekrotik ve dejeneratif değişiklikler saptandı. Bölünmüş kaspaz 3 gen ekspresyonu kontrol grubu ve TS grubunda azaldı (P<0.05). Ortalama testis biyopsi skoru (MTBS) TS grubunda en düşüktü (P<0.05). Gruplar arasında immünohistokimyasal (IHC) ve in situ hibridizasyon (IST) parametreleri açısından istatistiksel fark vardı. Sonuç olarak, HPE'nin sıçanların testis dokularında TS kaynaklı hasara karşı koruma sağlayabildiği görülmüştür.
Anahtar Kelimeler
Helichrysum plicatum oksidatif stres sıçan sperm talyum sülfat.
Kaynakça
- Akarsu SA, Türk G, Arkalı G, Çeribaşı AO, Yüce A. (2022). Changes in heavy metal levels, reproductive characteristics, oxidative stress markers and testicular apoptosis in rams raised around thermal power plant. Theriogenology, 179, 211-222.
- Akarsu SA, Gür C, İleritürk M, Akaras N, Küçükler S, Kandemir FM. (2023). Effect of syringic acid on oxidative stress, autophagy, apoptosis, inflammation pathways against testicular damage induced by lead acetate. Journal of Trace Elements in Medicine and Biology, 80, 127315.
- Aksu EH, Kandemir FM, Küçükler S. (2021). The effects of hesperidin on colistin‐induced reproductive damage, autophagy, and apoptosis by reducing oxidative stress. Andrologia, 53(2), e13900.
- Álvarez-Barrera L, Rodríguez-Mercado JJ, Mateos-Nava RA, Vázquez-Martínez Y, Altamirano-Lozano MA. (2019). Effect on the offspring of pregnant females CD-1 mice treated with a single thallium (I) application. Reproductive Toxicology, 90, 1-7.
- Apaydin Yildirim B, Kordali S, Terim Kapakin KA, Yildirim F, Aktas Senocak E, Altun S. (2017). Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats. Journal of Zhejiang University-Scıence B, 18(6), 501-11.
- Aslan M, Orhan DD, Orhan N, Sezik E, Yesilada E. (2007). In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats. Journal of Ethnopharmacology, 109(1), 54-9.
- Bayer R, Breitwieaser I, Ward J. (2007). Tribe Gnaphalieae (Cass.) Lecoq and Juillet (1831) In: Kadereit JW, Jeffrey C, editors. The Families and Genera of Vascular Plants, Asterales. In: Springer, Berlin.
- Belhan S, Özkaraca M, Kandemir FM, Gülyüz F, Yildirim S, Ömür AD, Yener Z. (2017). Effectiveness of hesperidin on methotrexate-induced testicular toxicity in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(5).
- Bhardwaj JK, Panchal H, Saraf P. (2021). Cadmium as a testicular toxicant: A Review. Journal of Applied Toxicology, 41(1), 105-17.
- Bigović DJ, Stević TR, Janković TR, Noveski NB, Radanović DS, Pljevljakušić DS, Djurić ZR. (2017). Antimicrobial activity of Helichrysum plicatum DC. Hemıjska Industrıja (Chemical Industry), 71(4), 337-42.
- Bramanti E, Onor M, Colombaioni L. (2018). Neurotoxicity induced by low thallium doses in living hippocampal neurons: evidence of early onset mitochondrial dysfunction and correlation with ethanol production. ACS Chemical Neuroscience, 10(1), 451-9.
- Chang Y, Tsai JF, Chen PJ, Huang YT, Liu BH. (2023). Thallium exposure interfered with heart development in embryonic zebrafish (Danio rerio): From phenotype to genotype. Science of The Total Environment, 878, 162901.
- Genchi G, Carocci A, Lauria G, Sinicropi MS, Catalano A. (2021). Thallium use, toxicity, and detoxification therapy: An overview. Applied Sciences, 11(18), 8322.
- Gibson JE, Becker BA. (1970). Placental transfer, embryotoxicity, and teratogenicity of thallium sulfate in normal and potassium-deficient rats. Toxicology and Applied Pharmacology, 16(1), 120-32.
- Gur C, Akarsu SA, Akaras N, Tuncer SC, Kandemir FM. (2023). Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite-induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats. Environmental Toxicology. 38(6),1265-76
- Gur C, Kandemir O, Kandemir FM. (2022). Investigation of the effects of hesperidin administration on abamectin‐induced testicular toxicity in rats through oxidative stress, endoplasmic reticulum stress, inflammation, apoptosis, autophagy, and JAK2/STAT3 pathways. Environmental Toxicology, 37(3), 401-12.
- Ileriturk M, Benzer F, Aksu EH, Yildirim S, Kandemir FM, Dogan T, Genc A. (2021). Chrysin protects against testicular toxicity caused by lead acetate in rats with its antioxidant, anti‐inflammatory, and antiapoptotic properties. Journal of Food Biochemistry 45(2), e13593.
- Ileriturk M, Kandemir O, Kandemir FM. (2022). Evaluation of protective effects of quercetin against cypermethrin‐induced lung toxicity in rats via oxidative stress, inflammation, apoptosis, autophagy, and endoplasmic reticulum stress pathway. Environmental Toxicology, 37(11), 2639-50.
- Khushboo M, Murthy MK, Devi MS, Sanjeev S, Ibrahim KS, Kumar NS, Gurusubramanian G. (2018). Testicular toxicity and sperm quality following copper exposure in Wistar albino rats: ameliorative potentials of L-carnitine. Environmental Science and Pollution Research, 25(2), 1837-62.
- Kulevanova S, Stefova M, Stafilov T. (2000). HPLC identification and determination of flavone aglycones in Helichrysum plicatum DC.(Asteraceae). Die Pharmazie, 55, 391–92.
- Kumar TR, Doreswamy K, Shrilatha B. (2002). Oxidative stress associated DNA damage in testis of mice: induction of abnormal sperms and effects on fertility. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 513(1-2), 103-11.
- Li D, Yao H, Du L, Zeng X, Xiao Q. (2022). Thallium (I and III) exposure leads to liver damage and disorders of fatty acid metabolism in mice. Chemosphere, 307, 135618.
- Metzstein MM, Stanfield GM, Horvitz HR. (1998). Genetics of programmed cell death in C. elegans: past, present and future. Trends in Genetics, 14(10), 410-6.
- Miloglu FD, Akpınar A, Güven L, Demirkaya AK, Gundogdu G, Nalcı KA, Hacımuftuoglu A. (2021). Evaluation the Effects of Helichrysum plicatum Subsp. pseudoplicatum on an In-Vitro Wound Model Using Human Dermal Fibroblast Cells. The International Journal of Lower Extremity Wounds, 15347346211016693.
- Sánchez-Chapul L, Santamaría A, Aschner M, Ke T, Tinkov AA, Túnez I, Rangel-López E. (2023). Thallium-induced DNA damage, genetic, and epigenetic alterations. Frontiers in Genetics, 14.
- Sevim Ç, Çomaklı S, Taghizadehghalehjoughi A, Özkaraca M, Mesnage R, Kovatsi L, Tsatsakis A. (2019). An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas. Toxicology Reports, 6, 42-50. Sindhi PI, Raval SH, Singh RD, Parmar RS, Patel JG, Patel BJ, Joshi DV. (2022). Thallium Induced Sub-chronic Toxicity Profile in Wistar Rats. Indian Journal of Veterinary Sciences and Biotechnology, 18(5), 93-9.
- Singh J, Kalamdhad AS. (2011). Effects of heavy metals on soil, plants, human health and aquatic life. Int J Res Chem Environ, 1(2), 15-21.
- Varão A, Silva J, Amaral L, Aleixo L, Onduras A, Santos C, Stiboller M. (2021). Toxic effects of thallium acetate by acute exposure to the nematode C. elegans. Journal of Trace Elements in Medicine and Biology, 68, 126848. Vermeulen K, Van Bockstaele DR, Berneman ZN. (2005). Apoptosis: mechanisms and relevance in cancer. Annals of Hematology, 84(10), 627-39.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Hekimlik |
| Bölüm | Orijinal Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Nisan 2024 |
| Gönderilme Tarihi | 2 Kasım 2023 |
| Kabul Tarihi | 5 Şubat 2024 |
| Yayımlandığı Sayı | Yıl 2024 Cilt: 17 Sayı: 1 |
Kaynak Göster
Van Health Sciences Journal (Van Sağlık Bilimleri Dergisi) başlıklı eser bu Creative Commons Atıf-Gayri Ticari 4.0 Uluslararası Lisansı ile lisanslanmıştır.
