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Research Article
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Year 2022, Volume: 44 Issue: 4, 338 - 342, 31.12.2022

Elif Eğilmez Cemile Zontul , Alakbar Huseynzada Gunel Aliyeva , Ulviyya Hasanova Ayça Taş , Yavuz Siliğ

https://doi.org/10.7197/cmj.1189765

Abstract

References

  • Bray, F., et al., Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. 2018. 68(6): p. 394-424.
  • Kasper, D., et al., Harrison's principles of internal medicine, 19e. Vol. 1. 2015: Mcgraw-hill New York, NY, USA.
  • Travis, W.D., E. Brambilla, and G.J.J.J.C.O. Riely, New pathologic classification of lung cancer: relevance for clinical practice and clinical trials. 2013. 31(8): p. 992-1001.
  • Bunn, P.A., Jr., Triplet combination chemotherapy and targeted therapy regimens. Oncology (Williston Park), 2001. 15(3 Suppl 6): p. 26-32.
  • Schabath, M.B., M.L.J.C.e. Cote, biomarkers, and prevention, Cancer progress and priorities: lung cancer. 2019. 28(10): p. 1563-1579.
  • Scagliotti, G., et al., Phase III study of carboplatin and paclitaxel alone or with sorafenib in advanced non-small-cell lung cancer. J Clin Oncol, 2010. 28(11): p. 1835-42.
  • Ciccia, A. and S.J. Elledge, The DNA damage response: making it safe to play with knives. Mol Cell, 2010. 40(2): p. 179-204.
  • Rosell, R., et al., Erlotinib versus standard chemotherapy as first-line treatment for European patients with advanced EGFR mutation-positive non-small-cell lung cancer (EURTAC): a multicentre, open-label, randomised phase 3 trial. 2012. 13(3): p. 239-246.
  • Abdel-Rahman, L.H., et al., Synthesis, physicochemical studies, embryos toxicity and DNA interaction of some new Iron (II) Schiff base amino acid complexes. 2013. 1040: p. 9-18.
  • Felip, E. and R.J.E.r.o.m.d. Rosell, Testing for excision repair cross-complementing 1 in patients with non-small-cell lung cancer for chemotherapy response. 2007. 7(3): p. 261-268.
  • Tomas, L. and D.W.J.S. Richard, Quality control by DNA repair. 1999. 286(5446): p. 1897-1905.
  • Fagbemi, A.F., B. Orelli, and O.D.J.D.r. Schärer, Regulation of endonuclease activity in human nucleotide excision repair. 2011. 10(7): p. 722-729.
  • Reed, E.J.C.t.r., Platinum-DNA adduct, nucleotide excision repair and platinum based anti-cancer chemotherapy. 1998. 24(5): p. 331-344.
  • Simon, G.R., et al., ERCC1 expression is a predictor of survival in resected patients with non-small cell lung cancer. 2005. 127(3): p. 978-983.
  • Cozzi, P.G.J.C.S.R., Metal–Salen Schiff base complexes in catalysis: practical aspects. 2004. 33(7): p. 410-421.
  • Martin, L.P., T.C. Hamilton, and R.J.J.C.c.r. Schilder, Platinum resistance: the role of DNA repair pathways. 2008. 14(5): p. 1291-1295.
  • Olaussen, K.A., et al., DNA repair by ERCC1 in non–small-cell lung cancer and cisplatin-based adjuvant chemotherapy. 2006. 355(10): p. 983-991.
  • Zhao, H., et al., Prognostic significance of BRCA1, ERCC1, RRM1, and RRM2 in patients with advanced non-small cell lung cancer receiving chemotherapy. 2014. 35(12): p. 12679-12688.
  • Olaussen, K.A., et al., DNA repair by ERCC1 in non-small-cell lung cancer and cisplatin-based adjuvant chemotherapy. 2006. 355 10: p. 983-91.

Investigation of The Effect of B-108 Contaınıng Azomethin Group On DNA Repair Gene

Year 2022, Volume: 44 Issue: 4, 338 - 342, 31.12.2022

Elif Eğilmez Cemile Zontul , Alakbar Huseynzada Gunel Aliyeva , Ulviyya Hasanova Ayça Taş , Yavuz Siliğ

https://doi.org/10.7197/cmj.1189765

Abstract

Objective: Cancer arises as a result of the failure of the mechanisms controlling normal division in a group of cells. It is known that some new synthesis compounds intended for use in cancer treatment have anti-fungal, anti-bacterial, anti-carcinogenic effects. In this study, it was aimed to apply the newly synthesized B-108 compound to the A-549 cell line and then to investigate the effect of this compound on the ERCC1 gene expression profile.
Materials and Methods: Firstly, compound B-108 was synthesized in our study. Afterwards, this synthesized molecule was applied in eight different concentrations (1-100 μg/ml) in A-549 cell line and 3-(4,5-dimethylthiazol-2-yl)-2,5-yl for 24 hours, 48 hours and 72 hours. Anticancer activities were determined using diphenyltetrazolium bromide (MTT) method. Expression level of DNA repair gene (ERCC1) was determined using RT-PCR method.
Results: As a result, it was determined that the molecule applied to the A-549 cell line showed the highest activity after 72 hours of incubation. It was observed that the ERCC1 gene expression of the molecule applied on lung cancer was lower than the control group.
Discussion: Considering the current study results, low expression of ERCC1 shows that compound B-108 correlates with overall survival on lung cancer cells.

Keywords

Azomethine Grup Lung cancer DNA Repair Gene Expression

References

  • Bray, F., et al., Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. 2018. 68(6): p. 394-424.
  • Kasper, D., et al., Harrison's principles of internal medicine, 19e. Vol. 1. 2015: Mcgraw-hill New York, NY, USA.
  • Travis, W.D., E. Brambilla, and G.J.J.J.C.O. Riely, New pathologic classification of lung cancer: relevance for clinical practice and clinical trials. 2013. 31(8): p. 992-1001.
  • Bunn, P.A., Jr., Triplet combination chemotherapy and targeted therapy regimens. Oncology (Williston Park), 2001. 15(3 Suppl 6): p. 26-32.
  • Schabath, M.B., M.L.J.C.e. Cote, biomarkers, and prevention, Cancer progress and priorities: lung cancer. 2019. 28(10): p. 1563-1579.
  • Scagliotti, G., et al., Phase III study of carboplatin and paclitaxel alone or with sorafenib in advanced non-small-cell lung cancer. J Clin Oncol, 2010. 28(11): p. 1835-42.
  • Ciccia, A. and S.J. Elledge, The DNA damage response: making it safe to play with knives. Mol Cell, 2010. 40(2): p. 179-204.
  • Rosell, R., et al., Erlotinib versus standard chemotherapy as first-line treatment for European patients with advanced EGFR mutation-positive non-small-cell lung cancer (EURTAC): a multicentre, open-label, randomised phase 3 trial. 2012. 13(3): p. 239-246.
  • Abdel-Rahman, L.H., et al., Synthesis, physicochemical studies, embryos toxicity and DNA interaction of some new Iron (II) Schiff base amino acid complexes. 2013. 1040: p. 9-18.
  • Felip, E. and R.J.E.r.o.m.d. Rosell, Testing for excision repair cross-complementing 1 in patients with non-small-cell lung cancer for chemotherapy response. 2007. 7(3): p. 261-268.
  • Tomas, L. and D.W.J.S. Richard, Quality control by DNA repair. 1999. 286(5446): p. 1897-1905.
  • Fagbemi, A.F., B. Orelli, and O.D.J.D.r. Schärer, Regulation of endonuclease activity in human nucleotide excision repair. 2011. 10(7): p. 722-729.
  • Reed, E.J.C.t.r., Platinum-DNA adduct, nucleotide excision repair and platinum based anti-cancer chemotherapy. 1998. 24(5): p. 331-344.
  • Simon, G.R., et al., ERCC1 expression is a predictor of survival in resected patients with non-small cell lung cancer. 2005. 127(3): p. 978-983.
  • Cozzi, P.G.J.C.S.R., Metal–Salen Schiff base complexes in catalysis: practical aspects. 2004. 33(7): p. 410-421.
  • Martin, L.P., T.C. Hamilton, and R.J.J.C.c.r. Schilder, Platinum resistance: the role of DNA repair pathways. 2008. 14(5): p. 1291-1295.
  • Olaussen, K.A., et al., DNA repair by ERCC1 in non–small-cell lung cancer and cisplatin-based adjuvant chemotherapy. 2006. 355(10): p. 983-991.
  • Zhao, H., et al., Prognostic significance of BRCA1, ERCC1, RRM1, and RRM2 in patients with advanced non-small cell lung cancer receiving chemotherapy. 2014. 35(12): p. 12679-12688.
  • Olaussen, K.A., et al., DNA repair by ERCC1 in non-small-cell lung cancer and cisplatin-based adjuvant chemotherapy. 2006. 355 10: p. 983-91.
There are 19 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Basic Science Research Articles
Authors

Elif Eğilmez 0000-0003-1125-7939

Cemile Zontul 0000-0002-1436-5145

Alakbar Huseynzada 0000-0002-6342-4260

Gunel Aliyeva 0000-0002-6995-8589

Ulviyya Hasanova 0000-0003-1502-4227

Ayça Taş 0000-0002-7132-1325

Yavuz Siliğ 0000-0002-0562-7457

Publication Date December 31, 2022
Acceptance Date December 19, 2022
Published in Issue Year 2022 Volume: 44 Issue: 4

Cite

AMA Eğilmez E, Zontul C, Huseynzada A, et al. Investigation of The Effect of B-108 Contaınıng Azomethin Group On DNA Repair Gene. CMJ. December 2022;44(4):338-342. doi:10.7197/cmj.1189765