Protective Effects of Lactobacillus plantarum Exopolysaccharides from Home Made Dairy Products on Obesity

Ahmet Turan Demir; Bilge Hilal Çadırcı; Ahmet Altun

doi:10.7197/cmj.1156620

EN

Protective Effects of Lactobacillus plantarum Exopolysaccharides from Home Made Dairy Products on Obesity

Abstract

As it is known, obesity is one of the most important health problems of developed and developing countries today. Obesity; endocrine, cardiovascular, respiratory, gastrointestinal (GI), genitourinary, musculoskeletal system such as body systems and psychosocial condition causes many health problems due to the negative effects. The ability of probiotics to support GI health has been recognized for years. Since alternative approaches against obesity can be developed by intervention in the GI system, this study aimed to evaluate the effects of exopolysaccharides (EPS) as an anti-obesity biomaterial isolated from natural products with probiotic characteristics. First, the one with the highest EPS amount among the 10 microorganisms with known probiotic properties was identified by MALDI-TOF and 16s rRNA sequence analysis and named as Lactobacillus plantrum BHC007. Then, the monosaccharide composition of the extracted EPS was examined, and its average molecular weight was determined as 221.860±0.006. The effect of EPS on the adipogenesis process was examined qualitatively with Oil Red O dye on the 3T3 cell line and cellular triacylglycerol (TAG) levels. It was concluded that the highest EPS concentration (2.25 mM) inhibited adipogenesis by 75%. Also, 17% inhibition of adipogenesis was achieved at the lowest EPS concentration (0.45 mM). In conclusion, TAG analysis revealed that EPS was suppressed depending on the lipid droplet formation concentration.

Keywords

Thanks

This study was supported with 100/2000 Ph.D Scholarship within the scope of Turkey Council of Higher Education 100/2000 Biomaterials and Tissue Engineering. In this regard, I would like to thank the Turkey Council of Higher Education.

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Ahmet Turan Demir ^*
0000-0002-3464-0466
Türkiye

Bilge Hilal Çadırcı
0000-0003-1525-9608
Türkiye

Ahmet Altun
0000-0003-2056-8683
Türkiye

Publication Date

October 1, 2022

Submission Date

August 5, 2022

Acceptance Date

September 28, 2022

Published in Issue

Year 2022 Volume: 44 Number: 3

DOI

https://doi.org/10.7197/cmj.1156620

IZ

https://izlik.org/JA79KB57JP

Cite

RIS / Bibtex

AMA

1.Demir AT, Çadırcı BH, Altun A. Protective Effects of Lactobacillus plantarum Exopolysaccharides from Home Made Dairy Products on Obesity. CMJ. 2022;44(3):273-281. doi:10.7197/cmj.1156620

Protective Effects of Lactobacillus plantarum Exopolysaccharides from Home Made Dairy Products on Obesity

Abstract

Keywords

Öz

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite