Investigation of The Effect of Compound B-47/2 Containing Azomethine Group On Angiogenesis

Efe Taha Bucak; Zuhal Tunçbilek; Alakbar Huseynzada; Mirjavid Aghayev; Ulviyya Hasanova; Ayça Taş; Yavuz Siliğ

doi:10.7197/cmj.1189799

EN

Investigation of The Effect of Compound B-47/2 Containing Azomethine Group On Angiogenesis

Abstract

Objective: Lung cancer is one of the most common cancers in the world. It is known that angiogenesis plays a role in the development and metastasis of lung cancer. Azomethine derivatives known as Schiff bases have many biological activities. In this study, we aimed to determine the anticancer activity of the newly synthesized azomethine derivative compound B-47/2 on lung cancer and to determine the effect of this component on vascular endothelial growth factor B (VEGFB) gene expression. Material and Method: Compound B-47/2 was synthesized for the first time. B-47/2 compound was applied to lung cancer cell line (A549) at varying concentrations (1-100 µg/mL) and its anticancer activity was found after 24, 48 and 72 hours incubations using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) method. The half maximal inhibitory concentration (IC50) dose of B-47/2 was applied to the cells and ribonucleic acid (RNA) isolation followed by complementary deoxyribonucleic acid (cDNA) synthesis was performed. Then, reverse transcription-polymerase chain reaction (RT-PCR) method was used to determine the expression level of VEGFB gene. Results: As a result, it was determined that the B-47/2 compound applied to the A-549 cell line showed the highest cytotoxic activity after 72 hours of incubation. In addition, it was determined that the B-47/2 compound decreased the expression of the VEGFB gene. Discussion: There are studies in which the anticancer activity of azomethine derivatives has been observed. The topic of synthesizing new drugs to prevent cancer is popular. We suggested that the newly synthesized component may have anticancer activity and may be effective on angiogenesis.

Keywords

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Efe Taha Bucak
0000-0002-3702-3404
Türkiye

Zuhal Tunçbilek ^*
0000-0002-6510-0884
Türkiye

Alakbar Huseynzada
0000-0002-6342-4260
Azerbaijan

Mirjavid Aghayev
0000-0003-0588-4450
Azerbaijan

Ulviyya Hasanova
0000-0003-1502-4227
Azerbaijan

Ayça Taş
0000-0002-7132-1325
Türkiye

Yavuz Siliğ
0000-0002-0562-7457
Türkiye

Publication Date

December 31, 2022

Submission Date

October 15, 2022

Acceptance Date

December 19, 2022

Published in Issue

Year 2022 Volume: 44 Number: 4

DOI

https://doi.org/10.7197/cmj.1189799

IZ

https://izlik.org/JA74SE26ZR

Cite

RIS / Bibtex

AMA

1.Bucak ET, Tunçbilek Z, Huseynzada A, et al. Investigation of The Effect of Compound B-47/2 Containing Azomethine Group On Angiogenesis. CMJ. 2022;44(4):343-347. doi:10.7197/cmj.1189799

Öz

Investigation of The Effect of Compound B-47/2 Containing Azomethine Group On Angiogenesis

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite