Does broccoli (brassica oleracea var. italica) extract have a potential therapeutic targets on human breast cancer (MDA-MB-231) using AgNOR detection method?

Gözde Çobanoğlu; Mustafa Nisari; Mehtap Nisari; Sümeyye Uçar; Gülderen Kerek; Neriman İnanç

doi:10.7197/cmj.1297877

EN

Does broccoli (brassica oleracea var. italica) extract have a potential therapeutic targets on human breast cancer (MDA-MB-231) using AgNOR detection method?

Abstract

In this study, it was aimed to determine the in vitro effect of the extract obtained from mature broccoli on the breast cancer cell line. MDA-MB231 breast cancer cell line was used in the study. MDA-MB-231 cells were exposed to broccoli extract at 37°C and 5% CO2 for varying durations (24 and 48 hours) and doses (125 and 250 µl/ml).At the end of the incubation period, viability, apoptosis, cell cycle and AgNOR protein status of MDA-MB-231 cells were examined in the Muse Cell Analyzer. In the groups containing broccoli extract, a decrease in the percentage of viable cells and a significant increase in the percentage of early and total apoptosis were observed for both doses compared to the control. In the cell cycle test, the number of cells in the S phase increased in all groups.It was observed that the groups containing broccoli extract slowed down the cell cycle in the transition to the S checkpoint. AgNOR staining results also supported cell cycle and apopitosis, and AgNOR number and TAA/NA ratio decreased in the 125 µl/ml broccoli extract group after 24-48 hours and were found to be statistically significant compared to the control group. It was determined that broccoli increased apoptosis on breast cancer cells by various mechanisms and inhibited cell viability/cell growth. The results were similar to the results of AgNOR protein synthesis. The study showed that the regular and correct consumption of broccoli could be effective in preventing cancer formation and slowing its progression.

Keywords

Supporting Institution

Nuh Naci Yazgan University

Project Number

2021/SA-LTP-3

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Gözde Çobanoğlu
0000-0002-7040-6212
Türkiye

Mustafa Nisari ^*
0000-0001-7469-8921
Türkiye

Mehtap Nisari
0000-0002-1126-7478
Türkiye

Sümeyye Uçar
0000-0003-3378-3745
Türkiye

Gülderen Kerek
0000-0002-8582-0395
Türkiye

Neriman İnanç
0000-0001-5026-4133
Türkiye

Publication Date

September 30, 2023

Submission Date

May 17, 2023

Acceptance Date

September 27, 2023

Published in Issue

Year 2023 Volume: 45 Number: 3

DOI

https://doi.org/10.7197/cmj.1297877

IZ

https://izlik.org/JA25MY43LC

Cite

RIS / Bibtex

AMA

1.Çobanoğlu G, Nisari M, Nisari M, Uçar S, Kerek G, İnanç N. Does broccoli (brassica oleracea var. italica) extract have a potential therapeutic targets on human breast cancer (MDA-MB-231) using AgNOR detection method? CMJ. 2023;45(3):44-56. doi:10.7197/cmj.1297877

Cited By

ANTICANCER EFFECTS OF BROCCOLI EXTRACT ON BREAST CANCER CELL LINES: A PHYTOCHEMICAL PERSPECTIVE

Asian Journal of Pharmaceutical and Clinical Research

https://doi.org/10.22159/ajpcr.2025v18i6.53925

Öz

Does broccoli (brassica oleracea var. italica) extract have a potential therapeutic targets on human breast cancer (MDA-MB-231) using AgNOR detection method?

Abstract

Keywords

Supporting Institution

Project Number

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

ANTICANCER EFFECTS OF BROCCOLI EXTRACT ON BREAST CANCER CELL LINES: A PHYTOCHEMICAL PERSPECTIVE