Evaluation of the Relationship of HBsAg Serum Plasma Values with HBV DNA and Other Serologic Markers in the Diagnosis of Hepatitis B

Hamdi Karahan; Cem Çelik

doi:10.7197/cmj.1524213

TR EN

Hepatit B Tanısında HBsAg Serum Plazma Değerlerinin HBV DNA ve Diğer Serolojik Belirteçlerle İlişkisinin Değerlendirilmesi

Öz

Amaç: Hepatit B virüsü (HBV) enfeksiyonları tüm dünyada çok önemli bir toplum sağlığı problemi oluşturmaktadır. HBV enfeksiyonunun tanı ve tedavisi önemli bir konudur. HBV DNA’nın polimeraz zincir reaksiyonu (PCR) yöntemi ile saptanarak viral replikasyonun gösterilmesi, serolojik ve biyokimyasal göstergelerle birlikte tanı ve tedavinin takibinde oldukça yararlıdır. Bu çalışmada, Hepatit B tanısında HBsAg serum konsantrasyon değerlerinin, HBV ile ilgili diğer serolojik parametreler ile karşılaştırılarak aralarındaki ilişkinin değerlendirilmesi amaçlanmıştır. Yöntem: Sivas Cumhuriyet Üniversitesi Uygulama ve Araştırma Hastanesi Mikrobiyoloji Laboratuvarına 2012–2020 yıllarında hastalardan alınan kan serumlarından elde edilen HBsAg, HBV DNA, Anti HBc IgG, Anti HBc IgM, HBeAg ve Anti HBe değerleri laboratuvar kayıtlarından geriye dönük olarak incelenmiştir. Bulgular: HBsAg serum titreleri ile HBV DNA arasında ve HBeAg ile HBV DNA arasında pozitif bir ilişki bulunmuştur. HBsAg titreleri arttıkça HBV DNA pozitiflik oranlarının arttığı görülmüştür. Aynı şekilde HBsAg titreleri arttıkça HBc IgG pozitifliklerinin arttığı tespit edilmiştir. Belirli bir noktaya kadar artan HBsAg titreleri ile HBeAg pozitifliği artarken, belirli bir titreden sonra HBeAg pozitifliği düşmüştür. HBV DNA sonuçları ile HBeAg sonuçları karşılaştırıldığında iki değer arasındaki ilişki önemli bulunmuştur. Sonuç: HBV enfeksiyonun doğru bir şekilde tanımlanmasında ve tedavinin izlenmesinde HBsAg’nin serum konsantrasyonlarının, HBV DNA ve diğer serolojik parametreler ile ilişkilerinin araştırılmasının yararlı olduğunu düşünmekteyiz.

Anahtar Kelimeler

Evaluation of the Relationship of HBsAg Serum Plasma Values with HBV DNA and Other Serologic Markers in the Diagnosis of Hepatitis B

Abstract

Objective: Hepatitis B virus (HBV) infections cause a major public health problem worldwide diagnosis and treatment of HBV infection is an important issue. Detection of HBV DNA by polymerase chain reaction (PCR) and demonstration of viral replication together with serologic and biochemical indicators are very useful in diagnosis and treatment. In this study, we aimed to evaluate the relationship between HBsAg serum concentration values and other serologic parameters related to HBV in the diagnosis of hepatitis B. Methods: HBsAg, HBV DNA, Anti HBc IgG, Anti HBc IgM, HBeAg, and Anti HBe values obtained from blood sera taken from patients in Sivas Cumhuriyet University Application and Research Hospital Microbiology Laboratory between 2012-2020 were retrospectively analyzed from laboratory records. Results: A positive correlation was found between HBsAg serum titers and HBV DNA and between HBeAg and HBV DNA. It was found that HBV DNA positivity rates increased as HBsAg titers increased. Similarly, it was found that HBc IgG positivity increased as HBsAg titers increased. HBeAg positivity increased with increasing HBsAg titers up to a certain point, while HBeAg positivity decreased after a certain titer. When HBV DNA results were compared with HBeAg results, the relationship between the two values was found to be significant. Conclusion: We think that it is useful to investigate the relationship between serum concentrations of HBsAg and HBV DNA and other serologic parameters in the accurate identification of HBV infection and monitoring of treatment.

Keywords

Ethical Statement

Each stage of the study was conducted with ethical principles. Before the application, the necessary permissions were obtained from the Sivas Cumhuriyet University Non-Interventional Clinical Research Ethics Committee (dated 25.05.2022, numbered 11/41)

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Details

Primary Language

English

Subjects

Health Services and Systems (Other)

Journal Section

Research Article

Authors

Hamdi Karahan
0000-0002-4801-2217
Türkiye

Cem Çelik ^*
0000-0002-7141-5874
Türkiye

Publication Date

September 30, 2024

Submission Date

July 29, 2024

Acceptance Date

September 10, 2024

Published in Issue

Year 2024 Volume: 46 Number: 3

DOI

https://doi.org/10.7197/cmj.1524213

IZ

https://izlik.org/JA63PP45PJ

Cite

RIS / Bibtex

AMA

1.Karahan H, Çelik C. Evaluation of the Relationship of HBsAg Serum Plasma Values with HBV DNA and Other Serologic Markers in the Diagnosis of Hepatitis B. CMJ. 2024;46(3):178-185. doi:10.7197/cmj.1524213