Evaluation of vaginal flora and susceptibility test of microorganisms in reproductive-age women with or without vaginitis in primary care settings

Aims: The treatment modalities of patients with vaginal discharge are generally related to their symptoms. The aim of this study was to evaluate vaginal flora and antibiogram analysis in reproductive-age women with or without vaginitis in primary care settings. Methods: Vaginal swabs were taken from 311 women who have vaginitis, and tested for the causative agents of vaginal discharge. The control group was 89 healthy women without vaginal discharge. Vaginal swaps were used in a commercial tests system. The tests were used for count and presumptive identification of the micro-organisms and for the susceptibility testing of the urogenital mycoplasmas in the sample were interpreted by assessing the change in color of the various wells and performing a microscope examination.

Results: Most isolated microorganisms were; Candida sp. from 39 (12.5%) cultures, Gardnerella vaginalis from 32 (10.2%) cultures, Staphylococcus aureus from 32 (10.2%) cultures, Ureaplasma urealyticum from 29 (9.3%) cultures, Trichomonas vaginalis was found in 21 (6.7%) of the vaginal fluid specimens, there was 27 (8.6%) cultures which has no isolation. In control group Candida sp. was isolated from 3 (3.3%) cultures, Gardnerella vaginalis from 3 (3.3%) cultures, Trichomonas vaginalis was found in 1 (1.1%) of the vaginal fluid specimens.

Conclusions: The results of this study confirm that culture and antibiogram are very important in the management of the vaginitis.

Abstract

Aims. The treatment modalities of patients with vaginal discharge are generally related to their symptoms. The aim of this study was to evaluate vaginal flora and antibiogram analysis in reproductive-age women with or without vaginitis in primary care settings. Methods. Vaginal swabs were taken from 311 women who have vaginitis, and tested for the causative agents of vaginal discharge. The control group was 89 healthy women without vaginal discharge. Vaginal swaps were used in a commercial tests system. The tests were used for count and presumptive identification of the microorganisms and for the susceptibility testing of the urogenital mycoplasmas in the sample were interpreted by assessing the change in color of the various wells and performing a microscope examination. Results. Most isolated microorganisms were Candida sp. from 39 (12.5%)cultures, Gardnerella vaginalis from 32 (10.2%) cultures, Staphylococcus aureus from 32 (10.2%) cultures, Ureaplasma urealyticum from 29 (9.3%) cultures, and Trichomonas vaginalis from 21 (6.7%) cultures of the vaginal fluid specimens, and in 27 (8.6%) cultures, there was no isolation. In control group, Candida sp. was isolated from 3 (3.3%) cultures, Gardnerella vaginalis from 3 (3.3%) cultures, Trichomonas vaginalis was found in 1 (1.1%) of the vaginal fluid specimens. Conclusion. The results of this study confirm that culture and antibiogram are very important in the management of the vaginitis.

Keywords: Vaginal flora, antibiogram, vaginitis, reproductive age

Özet

Amaç. Vajinal akıntısı olan hastalarda tedavi modaliteleri genellikle hastaların semptomları ile ilişkilidir. Bu çalışmanın amacı, birinci basamak sağlık kuruluşlarına başvuran, vajiniti olan ve olmayan doğurganlık çağındaki kadınlarda vajinal flora ve antibiyogram analizi sonuçlarını değerlendirmektir. Yöntem. Vajiniti olan 311 kadından vajinal sürüntü alındı ve vajinal akıntıya neden olan ajanlar araştırıldı. Kontrol grubu vajinal akıntısı olmayan 89 sağlıklı kadından oluşturuldu. Vajinal sürüntüler ticari test sisteminde değerlendirildi. Testler mikroorganizmaların sayısı ve olası identifikasyonları için kullanıldı, örnekteki ürogenital mikoplazmaların duyarlılık analizi çeşitli kuyucuklardaki renk değişikliği ve mikroskobik değerlendirme ile yapıldı. Bulgular. Vajinal sıvı spesmenlerinden en çok izole edilen mikroorganizmalar sırasıyla şunlardı: 39 kültürden (%12,5) Candida sp., 32 (%10,2) kültürden Gardnerella vaginalis, 32 (%10,2) kültürden Staphylococcus aureus, 29 (9,3%) kültürden Ureaplasma urealyticum ve 21 (%6,7) Trichomonas vaginalis. Yirmi yedi kültürde (%8,6) mikroorganizma izole edilmedi. Kontrol grubunda vajinal sıvı spesimenlerinden 3'ünde (%3,3) Candida sp., 3'ünde (%3,3) Gardnerella vaginalis, 1 (%1,1) Trichomonas vaginalis izole edildi. Sonuç. Bu çalışmanın sonuçları vajinit tedeavisinde kültür ve antibiyogramın çok önemli olduğunu doğrulamaktadır.

Anahtar sözcükler: Vajinal flora, antibiyogram, vaginit, üreme dönemi