Ateroskleroz ile il-1α (interleukin-1α -889 c/t) gen polimorfizmi arasındaki ilişkinin araştırılması

Hasan Basçil; Erhan Atahan; Müslim Gül; Serdal Arslan; Nil Ozbilum; Öcal Berkan

doi:10.7197/cmj.v36i2.5000012436

EN TR

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Abstract

Aim. Atherosclerosis is regarded as a chronic inflammatory disease developing in response to injury in the vessel wall. Inflammation plays a key role in the development of atherosclerosis. Interleukin- 1α (IL-1α) has a critical role on regulation of proinflammation. The aim the study was to assess the relationship between the IL-1α -889 C/T polymorphism and the atherosclerosis. Method. The study population was composed of 117 patients (Group I) and 117 healthy adults (Group II). Genomic DNA of case and control was extracted from blood leukocytes. IL-1α genotype were further confirmed by direct sequencing of randomly selected samples. Results. 78 male and 39 female individuals in the group I, 49 men and 68 women in Group II. The average age of individuals in the Group I 61.06, the average age of individuals in the Group II 59.47 years old. The Group I 43 and the Group II were detected in 28 individuals with high cholesterol(total cholesterol>200 mg/dL). 62 individuals in the Group I and 51 individuals in the Group II was smoking. The Group I 81 individuals with high blood pressure(systolic blood pressure>140 mmHg and / or diastolic blood pressure>90 mmHg), 42 individuals with diabetes, the Group II 32 individuals with high blood pressure, diabetes was found 41 individuals. In the atherosclerotic coronary artery patients of %54.70 individuals carrying the CC genotype, %35.04 individuals carrying the CT genotype and %10.25 individuals carrying the TT genotype constitutes. In the healty control group, %59.82 individuals carrying the CC genotype, %30.76 individuals carrying CT genotype and %9.40 individuals carrying TT genotype. IL-1α polymorphism of C allele frequency is %75.21 in the Group II and %72.22 in the Group I. T allele freguency distribution of %24.78 in the Group II and %27.77 in the Group I. Conclusion. Those with coronary artery disease group between the Group II age, sex distribution, hypertension and hypercholesterolemia were found statistically significant relationship. There was no statistically significant difference between groups according to the IL-1α-889 C/T gene polymorphism.

Keywords

Coronary atherosclerosis; IL-1α gene polymorphism; risk factors

Ateroskleroz ile il-1α (interleukin-1α -889 c/t) gen polimorfizmi arasındaki ilişkinin araştırılması

Öz

Amaç. Genetik ve çevresel faktörler arasındaki ilişki sonucu ortaya çıkan kardiyovasküler hastalıkların gelişiminde inflamasyon anahtar rol oynamaktadır. İnterlökin-1α (IL-1α) nın proinflamasyon regülasyonunda önemli rolü vardır. Bu çalışmada IL-1α-889 C/T polimorfizmi ile ateroskleroz arasındaki ilişkinin araştırılması amaçlanmıştır. Yöntem. Bu çalışma popülasyonu 117 hasta (Grup I) ve 117 sağlıklı (Grup II) bireyden oluşmuştur. Grup I ve Grup II deki bireylerin genomik DNA’sı izole edildi. IL-1α genotipleri rastgele seçilen örneklerden direkt dizi analizi yapılarak doğrulandı. Bulgular. Grup I 78 erkek ve 39 kadın bireyden, Grup II ise 49 erkek ve 68 kadın bireyden oluşmaktadır. Grup I bireylerin yaş ortalaması 61,06; Grup II bireylerin yaş ortalaması ise 59,47’dir. Grup I’de 43 bireyde, Grup II’de ise 28 bireyde yüksek kolesterol (total kolesterol > 200 mg/dL) bulundu. Grup I’de 62 bireyde, Grup II’de 51 bireyde sigara içiciliği bulundu. Grup I’de 81 bireyde yüksek tansiyon ( sistolik kan basıncı > 140 mmHg ve/veya diyastolik kan basıncı > 90 mmHg), 42 bireyde diyabet, Grup II’de 32 bireyde yüksek tansiyon, 41 bireyde diyabet bulundu. Aterosklerotik Grup I’de CC genotip taşıyan bireyler grubun %54,70’ini, CT genotip taşıyan bireyler %35,04’nü ve TT genotip taşıyan bireyler ise %10,25’ini oluşturmaktadır. Grup II’de ise bu oranlar CC genotip taşıyanlarda %59,82; CT genotip taşıyanlarda %30,76 ve TT genotip taşıyanlarda %9,40 olarak saptanmıştır. IL-1α polimorfizmi için C allel frekansı Grup II’de %75,21 ve Grup I’de %72,22’dir. T allel frekansı dağılımı Grup II’de %24,78 ve Grup I’de ise %27,77’dir. Sonuç. Koroner Arter hastalığı olan Grup I ile, kontrol grubu olan Grup II arasında yaş, cinsiyet dağılımı, hipertansiyon ve hiperkolesterolemi açısından istatistiksel olarak anlamlı bir ilişki saptanmıştır. Grup I ile Grup II arasında IL-1α-889 C/T gen polimorfizmi açısından istatistiksel olarak anlamlı bir ilişki saptanmamıştır.

Anahtar Kelimeler

Koroner ateroskleroz, IL-1α gen polimorfizmi, risk faktörleri

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Details

Primary Language

Turkish

Subjects

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Journal Section

-

Authors

Hasan Basçil

Erhan Atahan

Müslim Gül

Serdal Arslan

Nil Ozbilum

Öcal Berkan

Publication Date

March 29, 2014

Submission Date

March 29, 2014

Acceptance Date

-

Published in Issue

Year 2014 Volume: 36 Number: 2

DOI

https://doi.org/10.7197/cmj.v36i2.5000012436

IZ

https://izlik.org/JA58HA25UW

Cite

RIS / Bibtex

AMA

1.Basçil H, Atahan E, Gül M, Arslan S, Ozbilum N, Berkan Ö. Ateroskleroz ile il-1α (interleukin-1α -889 c/t) gen polimorfizmi arasındaki ilişkinin araştırılması. CMJ. 2014;36(2):231-240. doi:10.7197/cmj.v36i2.5000012436