Investigation of anti-epileptic mechanisms of 5HT1A receptor with pentylenetetrazole induced epilepsy model in rats

Bilal Şahin; Ercan Özdemir; Ahmet Şevki Taşkıran; Erkan Gümüş; Mustafa Ergül

doi:10.7197/cmj.vi.623515

EN

Investigation of anti-epileptic mechanisms of 5HT1A receptor with pentylenetetrazole induced epilepsy model in rats

Abstract

Objective: According to current neurophysiological evidence, the role of 5-hydroxytryptamine (5-HT) receptors in epileptic seizure formation is still not fully elucidated. The aim of this study was to investigate the effects of 5-HT_1A receptor on epileptic seizure with pentylenetetrazole induced epilepsy in rats.

Method: In this study, 28 male Wistar Albino rats weighing 240-260 g were used. Pentylenetetrazole (PTZ, 35 mg/kg, i.p.) was injected to the rats to induce epilepsy and seizure stages were determined according to the Racine scale. Electrodes were placed in the skulls of the animals under stereotaxis for ECoG recording. All the experimental animals were sacrificed by decapitation after ECoG and video recordings. GABA level was measured using the Elisa kit from brain tissues, and c-Fos expression was shown immunohistochemically.

Results: According to the results, it was shown that 8-OH-DPAT increased the time of initial myoclonic jerk (FMJ (p<0.05). However, the number of epileptic spikes was reduced by 8-OH-DPAT (p<0.05). GABA levels decreased in PTZ group (p<0.05). 8-OH-DPAT and WAY-100135 decreased c-Fos expression in all hippocampal areas (p<0.05).

Conclusions: In conclusion, 5-HT_1A receptor agonist 8-OH-DPAT, showed an anti-epileptic effect. The anti-epileptic effects of 5-HT_1Areceptor were found to be inconsistent with changes in GABA level. c-Fos expression is a marker of neuronal activation and may be related to the anti-convulsive effect of 5-HT_1A receptor.

Keywords

Epilepsy,pentylenetetrazole,serotonin,GABA,c-Fos,serotonin receptor agonist

Supporting Institution

Sivas Cumhuriyet Üniversitesi

Project Number

T-739

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Bilal Şahin ^*
0000-0002-4419-1385
Türkiye

Ercan Özdemir
0000-0001-8231-1053

Ahmet Şevki Taşkıran
0000-0002-5810-8415

Erkan Gümüş
0000-0001-6432-7457

Mustafa Ergül
0000-0003-4303-2996

Publication Date

September 30, 2019

Submission Date

September 23, 2019

Acceptance Date

September 26, 2019

Published in Issue

Year 2019 Volume: 41 Number: 3

DOI

https://doi.org/10.7197/cmj.vi.623515

IZ

https://izlik.org/JA52LU97HU

Cite

RIS / Bibtex

AMA

1.Şahin B, Özdemir E, Taşkıran AŞ, Gümüş E, Ergül M. Investigation of anti-epileptic mechanisms of 5HT1A receptor with pentylenetetrazole induced epilepsy model in rats. CMJ. 2019;41(3):490-499. doi:10.7197/cmj.vi.623515