EN
Synthesis of crosslinked PVA/Chitosan hydrogels loaded EGF and evaluation in vitro characterization
Abstract
Objective: The aim of this study was to prepare different hydrogels using chitosan and PVA polymers and to evaluate the cytotoxicity of hydrogels through cell culture studies. In addition, FTIR spectrum measurements were aimed to determine the specific chemical groups and bonds in the structures of hydrogels and materials.
Method: In this study, different hydrogels were prepared and 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl tetrazolium bromide (MTT) test was performed to evaluate the toxicity of hydrogels on mouse fibroblast cell line (L-929). In addition, specific chemical groups of materials and hydrogels used in hydrogels were determined via FTIR spectrum study.
Results: According to the MTT cytotoxicity results B1 (94.20±1.30 %) and D2 (82.45±1.05 %) formulations showed the greatest and lowest L-929 cell viability respectively. According to the results of the FTIR absorption spectrum, the aliphatic C = O tension band of rh-EGF was observed at 1634 cm-1.
Conclusions: Results showed that chitosan-PVA hydrogels containing r-EGF types were observed to increase mouse fibroblast cell viability. In the FTIR spectrum results, spectral bands showing specific chemical groups of chitosan, PVA, PEGylation agents, rh-EGF and C2 formulation were obtained.
Keywords
Supporting Institution
Marmara Üniversitesi Bilimsel Araştırmalar Projesi
Project Number
BAPKO, SAG-C-DRP-131216-0537
References
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Details
Primary Language
English
Subjects
Health Care Administration
Journal Section
Research Article
Publication Date
May 20, 2020
Submission Date
March 9, 2020
Acceptance Date
May 11, 2020
Published in Issue
Year 1970 Volume: 42 Number: 1