Research Article

Glutathione improves the prognosis of intrauterine growth restriction via downregulated hepatic and renal TNFα expression in Wistar rats

Volume: 43 Number: 4 December 31, 2021
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Cumhuriyet Medical Journal
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Glutathione improves the prognosis of intrauterine growth restriction via downregulated hepatic and renal TNFα expression in Wistar rats

Abstract

Objective: Oxidative stress is requisite for the development of IUGR, with subsequent manifestation in the reduced levels of antioxidants enzymes. Glutathione protects against free radical-mediated injury, therefore we elucidated on its role in protecting against oxidative stress-induced consequences of IUGR.
In order to improve the prognosis of IUGR in affected infants, we investigated the protective role of glutathione in rats exposed to IUGR.
Method: Ten female Sprague-Dawley rats were mated overnight. The pregnant rats were divided into 2 groups of 5 rats each. From gestational day 9 until parturition, group A received normal saline while group B received 50 mg/kg daily of L-NAME. Pups from group A were allowed free access to food and water, while group B pups were randomly assigned into 3 groups; G1 pups were left untreated; 1.5g/kg of gluthatione was administered to G2 pups from PND 4-10 and G3 pups from PND 25-31. We measured the body weight of rats; immunolocalized and further quantified TNFα expression in the hepatic and renal tissues.
Results: Postnatal GSH administration increased body weight in treated groups exposed to IUGR more significantly from days 4-10 as opposed to days 25-31. IUGR resulted in a significant increase in the TNFα immunoreactivity in the hepatic and renal tissues of the untreated group of rats when compared with the control and treated groups. GSH significantly reduced TNFα immunoreactivity in the kidney and liver of the treated groups, especially the days 4-10.
Conclusions: Oral GSH administration regulates the inflammatory response in IUGR at the early neonatal period.

Keywords

Supporting Institution

International Brain Organization- African Regional Committee

Project Number

Not applicable

References

  1. Dr Ijomone KO Department of Anatomy, University of Medical Sciences, Ondo Olaibio27@gmail.com

Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Philemon Shallie
0000-0001-9910-3584
Nigeria

Olawande Bamisi
0000-0001-7625-2456
Nigeria

Publication Date

December 31, 2021

Submission Date

January 13, 2021

Acceptance Date

December 27, 2021

Published in Issue

Year 2021 Volume: 43 Number: 4

DOI

https://doi.org/10.7197/cmj.860101

IZ

https://izlik.org/JA54FT49LT

Cite

RIS / Bibtex
AMA
1.Alese M, Shallie P, Bamisi O. Glutathione improves the prognosis of intrauterine growth restriction via downregulated hepatic and renal TNFα expression in Wistar rats. CMJ. 2021;43(4):330-338. doi:10.7197/cmj.860101