Abstract
Objective: The aim of this study was to prepare different nanoparticles using chitosan and tripolyphosphate and to perform characterization studies of nanoparticles. In addition, FTIR spectrum measurements were aimed to determine specific chemical groups and bonds in the structures of nanoparticles and materials.
Method: In this study, different nanoparticles were prepared and their zeta potential, particle size and polydispersity index were measured. Moreover, specific chemical groups of nanoparticles and materials used in nanoparticles were determined by FTIR spectrum study. Morphological properties of nanoparticles were evaluated using scanning electron microscopy.
Results: According to the zeta potential measurement results, A3 (18.66 ± 0.22) and A2 (14.32 ± 0.14) formulations showed the highest and the lowest zeta potential value, respectively. It was also observed that the A3 (284.22 ± 1.60) formulation had the lowest particle size. According to FTIR absorption spectrum results, a wide aliphatic O-H tension band of chitosan was observed between 1500 cm-1 -1100 cm-1.
Conclusions: According to the results, it could be said that chitosan nanoparticles have the desired particle size, charge and morphological properties in terms of application. In FTIR spectrum results, bands showing specific chemical groups of chitosan, tripolyphosphate and A3 formulation were obtained.
Keywords
References
- Fan W, Yan W, Xu Z. Formation mechanism of monodisperse, low molecular weight chitosan nanoparticles by ionic gelation technique. Colloids Surf B 2012; 90:21–7.
- Lam TD, Hoang VD, Lien LN, Thinh NN, Dien PG. Synthesis and characterization of chitosan nanoparticles used as drug. J. Chemistry, 2006; 44:105–109.
- Jing ZW, Jia YY, Wan N. Design and evaluation of novel pH-sensitive ureido conjugated chitosan/TPP nanoparticles targeted to helicobacter pylori. Biomaterials 2016; 84:276–85.
- Sun Y, Wan A. Preparation of nanoparticles composed of chitosan and its derivatives as delivery systems for macromolecules. J Appl Polym Sci 2007; 105:552–61.
- Retamal Marin RR, Babick F, Hillemann L. Zeta potential measurements for non-spherical colloidal particles–practical issues of characterisation of interfacial properties of nanoparticles. Colloids Surf A 2017; 532:516–21.
- Csaba N, KopingHoggard M, Alonso M J. Ionically cross-linked chitosan/ tripolyphosphate nanoparticles for oligonucleotide and plasmid DNA delivery. Int J Pharmaceutics, 2009; 382:05-14.
- Koukaras KN, Papadimitriou SA, Bikiaris DN, Froudakis GE. Insight on the Formation of Chitosan Nanoparticles through Ionotropic Gelation with Tripolyphosphate Molecular J Pharmaceutics, 2012; 9:2856−2862.
- Yang W, Fu J, Wang T, He N. Chitosan/sodium tripolyphosphate nanoparticles: preparation, characterization and application as drug carrier. J. Biomedical Nanotechnology, 2009; 5: 591–595.
- Vino AB, Ramasamy P, Shanmugam V, Shanmugam A. Extraction, characterization and in vitro antioxidative potential of chitosan and sulfated chitosan from Cuttlebone of Sepia aculeata Orbigny, 1848. Asian. Pac. J. Trop. Biomed. 2012; 2: 334–S341.
- Song C, Yu H, Zhang M, Yang Y, Zhang G. Physicochemical properties and antioxidant activity of chitosan from the blowfly Chrysomya megacephala larvae. Int. J. Biol. Macromol. 2013; 60: 347–354.
- Melo-Silveira, R.F.; Fidelis, G.P.; Costa, M.S.S.P.; Telles, C.B.S.; Dantas-Santos, N.; Elias, S.O.; Ribeiro, V.B.; Barth, A.L.; Macedo, A.J.; Leite, E.L.; et al. In Vitro Antioxidant, Anticoagulant and Antimicrobial Activity and in Inhibition of Cancer Cell Proliferation by Xylan Extracted from Corn Cobs. Int. J. Mol. Sci. 2012, 13, 409–426.
Abstract
References
- Fan W, Yan W, Xu Z. Formation mechanism of monodisperse, low molecular weight chitosan nanoparticles by ionic gelation technique. Colloids Surf B 2012; 90:21–7.
- Lam TD, Hoang VD, Lien LN, Thinh NN, Dien PG. Synthesis and characterization of chitosan nanoparticles used as drug. J. Chemistry, 2006; 44:105–109.
- Jing ZW, Jia YY, Wan N. Design and evaluation of novel pH-sensitive ureido conjugated chitosan/TPP nanoparticles targeted to helicobacter pylori. Biomaterials 2016; 84:276–85.
- Sun Y, Wan A. Preparation of nanoparticles composed of chitosan and its derivatives as delivery systems for macromolecules. J Appl Polym Sci 2007; 105:552–61.
- Retamal Marin RR, Babick F, Hillemann L. Zeta potential measurements for non-spherical colloidal particles–practical issues of characterisation of interfacial properties of nanoparticles. Colloids Surf A 2017; 532:516–21.
- Csaba N, KopingHoggard M, Alonso M J. Ionically cross-linked chitosan/ tripolyphosphate nanoparticles for oligonucleotide and plasmid DNA delivery. Int J Pharmaceutics, 2009; 382:05-14.
- Koukaras KN, Papadimitriou SA, Bikiaris DN, Froudakis GE. Insight on the Formation of Chitosan Nanoparticles through Ionotropic Gelation with Tripolyphosphate Molecular J Pharmaceutics, 2012; 9:2856−2862.
- Yang W, Fu J, Wang T, He N. Chitosan/sodium tripolyphosphate nanoparticles: preparation, characterization and application as drug carrier. J. Biomedical Nanotechnology, 2009; 5: 591–595.
- Vino AB, Ramasamy P, Shanmugam V, Shanmugam A. Extraction, characterization and in vitro antioxidative potential of chitosan and sulfated chitosan from Cuttlebone of Sepia aculeata Orbigny, 1848. Asian. Pac. J. Trop. Biomed. 2012; 2: 334–S341.
- Song C, Yu H, Zhang M, Yang Y, Zhang G. Physicochemical properties and antioxidant activity of chitosan from the blowfly Chrysomya megacephala larvae. Int. J. Biol. Macromol. 2013; 60: 347–354.
- Melo-Silveira, R.F.; Fidelis, G.P.; Costa, M.S.S.P.; Telles, C.B.S.; Dantas-Santos, N.; Elias, S.O.; Ribeiro, V.B.; Barth, A.L.; Macedo, A.J.; Leite, E.L.; et al. In Vitro Antioxidant, Anticoagulant and Antimicrobial Activity and in Inhibition of Cancer Cell Proliferation by Xylan Extracted from Corn Cobs. Int. J. Mol. Sci. 2012, 13, 409–426.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Medical Science Research Articles |
| Authors | |
| Publication Date | October 27, 2020 |
| Acceptance Date | October 26, 2020 |
| Published in Issue | Year 2020 |